Profiling the effects of microwave-assisted and soxhlet extraction techniques on the physicochemical attributes of Moringa oleifera seed oil and proteins

There is a constant search for biomaterials from natural products like plants for food and industrial applications.The work embodied in this report aimed at investigating the effects of microwave-assisted and soxhlet extraction(MAE and SE) techniques on the functional physicochemical quality characteristics of Moringa oleifera seed oil and proteins extracts. M. oleifera seeds were ground to fine powders and oil was extracted by microwave-assisted and soxhlet extraction techniques using petroleum ether. Quality attributes including yield percent, moisture content,iodine, saponification, specific gravity, viscosity, p H, thiobarbituric acid, acid and peroxide values were measured. Mineral and vitamin contents, chemical/functional groups, fatty acid(FA) composition, and reducing power of the oil were evaluated. Metabolomics of protein extracted from the defatted powders were analyzed by nuclear magnetic resonance(NMR). M. oleifera oil from MAE and SE methods had good yield(34.25 ± 0.0%,28.75 ± 0.0%), low moisture content(0.008 ± 0.0%, 0.011 ± 0.0%), non-drying and unsaturated, moderately saponified, less dense(0.91 ± 0.01, 0.92 ± 0.02 g m L^(-1)), had Newtonian flow, were weakly acidic, showed good content of FAs, recorded strong potential for long shelf-life, showed stability against oxidative rancidity and enzymatic hydrolysis, had very rich deposits of micro-and macro-nutrients as well as water-soluble and lipidsoluble vitamins, and functional groups in the oil were reflective of its content of long-and medium-chain triglycerides(LCT and MCT). Monounsaturated and saturated fatty acids(MUFA and SFA) were detected and the oil has excellent ferric ion reducing power. NMR metabolomic assay revealed the presence of nine essential amino acids(EAAs) in the protein extract. MAE technique is a feasible and acceptable alternative for high throughput extraction of M. oleifera oil with high yield and excellent quality attributes. The study revealed that MAE did not impart any remarkable advantage(s) on the physicochemical properties of M. oleifera seed oil and protein compared to SE technique.

Effects of a Cocktail Supplement of Ginkgo Biloba and Acai Extract on Cognitive Symptoms of Parkinson’s Disease

Parkinson’s Disease (PD) is a neurodegenerative disorder characterized by motor and non-motor symptoms, including cognitive impairment. Current treatments often involve synthetic drugs with significant side effects and potential for dependency. This study investigates the effects of a natural supplement combination of Ginkgo Biloba and Acai Extract on cognitive symptoms in a 77-year-old male with PD. The participant underwent a three-month supplementation regimen, with cognitive function assessed using the Montreal Cognitive Assessment (MoCA) test before and after the intervention. The results indicated an improvement in cognitive scores, suggesting that the combination of Ginkgo Biloba and Acai Extract may offer a promising alternative or adjunct to conventional PD treatments. This study highlights the potential of natural supplements in managing PD symptoms and calls for further research with larger sample sizes to confirm these findings. Human data was performed in accordance with the Declaration of Helsinki by the Roxbury District IRB Board (IRB Number: IRB00011767).

Effect of Temperature on Extraction of Castor Oil from Castor Seeds Using Potential Green Solvents

Extraction of castor oil from castor seeds was investigated using different green solvents which include d-limonene, p-cymene, α-pinene, ethanol, and furfural at the temperature range of (323 - 413) K. The Soxhlet extraction method was employed to investigate the effect of temperature at atmospheric pressure. The focus of the study was to investigate a potential green solvent that can produce the high yields compared to the traditional solvent (hexane). The results show that at the average time of 3 hours and 30 minutes, the castor oil yield for green solvents were ranked as furfural (47.13%) > ethanol (45.37%) > p-cymene (39.15%) > d-limonene (39.13%) > α-pinene (38.11%). These castor oil yields were obtained at optimum temperatures for each green solvent. The castor oil yields were compared to the yield of hexane (31.36%) at same average time. The green solvents were recovered by using simple distillation, except furfural which was difficult to be recovered.

Melt extraction and mineralization: A case study from the Shuangjianzishan supergiant Ag-Pb-Zn deposit(208 Mt), Inner Mongolia, NE China

The supergiant Shuangjianzishan(SJZ) Ag-Pb-Zn deposit is in the southern segment of the Great Hinggan Range(SGHR), northeast China. Previous studies suggest the ore-forming material and fluid originated from the magmatic system, and the mineralization age was consistent with the diagenetic age. However,the relationship between granitic magmatism and mineralization is still unclear in the SJZ. In this study, CH-O-He-Ar and in-situ S-Pb isotope analyses were conducted to determine the sources of ore-forming fluids and metals, which were combined with geochemistry data of SJZ granitoids from previous studies to constrain the relationship between the magmatism and the mineralization. The C-H-O-He-Ar-S-Pb isotopic compositions suggested the SJZ ore-forming material and fluids were derived from a magmatic source, which has mixed a small amount of mantle-derived materials. In addition, the disseminated sulfide from the syenogranite has comparable S-Pb isotopic composition with the sulfide minerals from ore veins,suggesting that the generation of the SJZ ore-forming fluids has a close relationship with the syenogranite magmatism. Combining with the geochemical characters of the syenogranite, the authors proposed that the mantle-derived fingerprint of the SJZ ore-forming fluid might be caused by the parent magma of the syenogranite, which was derived from partial melting of the juvenile lower crust, and underwent the residual melts segregated from a crystal mush in the shallow magma reservoir. The extraction of the syenogranite parent magma further concentrated the fertilized fluids, which was crucial to mineralization of the SJZ Ag-Pb-Zn deposit.

Discoursing on Soxhlet extraction of ginseng using association analysis and scanning electron microscopy

Soxhlet extraction is a common method of sample preparation. However, there has been no discussion about the efficiency of Soxhlet extraction from different batches and the factors that cause content fluctuation. In this study, Panax ginseng was selected as a model sample. Soxhlet extraction by means of a water bath, which has always been neglected, was identified as a novel key factor in the poor repeat- ability in different batches of Soxhlet extraction, as it can affect the siphon times and reflux time, which have been positively correlated with the ginsenoside contents. By substituting round bottom flasks in the same column, the relative standard deviation of the most fluctuated compound, ginsenoside Rbl, was decreased from 24.6% to 5.02%. Scanning electron microscopy analysis confirmed that the breakdown of the surface of the ginseng powder in the Soxhlet extraction led to a better dissolution of ginsenosides, indicating that chloroform may promote the extraction of ginsenosides by disrupting the cell structure. Moreover, 70% methanol was regarded as the better solvent for extracting the ginsenosides. Overall, this work offers a practical and effective protocol for improving the accuracy and repeatability of Soxhlet extraction methodology for ginsenosides and other analytes.

Extraction of Organochlorine Pesticides in Sediments Using Soxhlet,Ultrasonic and Accelerated Solvent Extraction Techniques

The application of soxhlet, ultrasonic and accelerated solvent extractiontechniques to the analysis of six organochlorine pesticides (α-HCH, β-HCH, γ-HCH, o, p'-DDT,p,p'-DDT and p,p'-DDE) in Taihu Lake sediment samples is described. It was found that the limits ofquantification ranged from 0.002 μg g^(-1) to 0.004 μg g^(-1), and the recoveries oforganochlorine pesticides with the three extraction techniques were acceptable ( 】80.7%). With amass selective detector, better results were obtained by accelerated solvent extraction usinghexane-acetone (1:1) as compared with soxhlet and ultrasonic extraction. It was shown that theaccelerated solvent extraction was the optimum technique for the analysis of organochlorinepesticides in sediments. The general features of the three extraction techniques are also presented.

Extraction of the Residual Polycyclic Aromatic Hydrocarbons in the Long-term Contaminated Soil in the Field

[Objective] The aim of this study was to find a better method to exactly quantify the residual polycyclic aromatic hydrocarbons（PAHs）in the long-term contaminated soil in the field so as to provide basis for the remediation for PAHs contaminated soil.[Method]The comparisons of soxhlet extraction,ultrasonic extraction and shaking extraction were studied in the agricultural soil irrigated with oil sewage over a long period of time.[Result]The total PAHs extracted by soxhlet extraction were the highest（6 873.7 μg/kg）,followed by shaking extraction（6 698.8 μg/kg）and ultrasonic extraction（5 737.6 μg/kg）.Among these methods,the highest extracted 4 and 6 rings PAHs were in the soxhlet extraction,the highest extracted 2 and 5 rings PAHs were in the shaking extraction,and the highest extracted 3 rings PAHs were in the ultrasonic extraction.[Conclusion]This study had provided basis for the selection of the assessment method of residual PAHs in the field soil.

Applicability of Static Supercritical Carbon Dioxide Extraction in Biogeochemical Characterization of Oil Shales

This study aimed at the assessment of applicability of static supercritical carbon dioxide extraction method (SFE) in biogeochemical characterization of oil shales as an alternative to the standard Soxhlet extration. A comparative investigation on yields and compositions of the solvent soluble bitumoids and their constituents extracted from Estonian Kukersite and Dictyonema oil shales by using Soxhlet extraction method (SEM) and static CO2 supercritical fluid extraction in an autoclave at varied subpyrolysis temperatures was carried out. Resulting from TLC- and GC-MS-analyses, aliphatic and aromatic hydrocarbons and neutral oxygen compounds were separated and identified. For the first time, in the composition of the Kukersite bitumoid, homologous series of n-alkanones-3 to n-alkanones-7 were detected. The extracts obtained were similar in both group and individual composition, and geochemical parametres calculated on the basis of aliphatic hydrocarbons including that made static SFE applicable to geochemical investigation of oil shales.

Zinc extraction from sewage sludge with [S,S]-EDDS

An environmentally friendly biodegradable chelant,[S,S]-ethylenediaminedisuccinic acid(EDDS),was used to separate heavy metals from sewage sludge based on chemical extraction technology.The extraction of zinc from sewage sludge with an aqueous solution of EDDS was studied under various conditions.It is found that the extraction efficiency of zinc using EDDS is equivalent to that using ethylenediaminetetraacetic acid(EDTA)at the molar ratio of chelant to total heavy metals of 1:1 over pH range of 5-9.EDDS is capable of extracting zinc from the sludge.The extraction efficiency is improved obviously and reaches a higher value within pH range from 3 to 10.The extraction efficiency maintains at about 65% when pH≥4.5 and the ratio of 10:1.By comparing the species contents of the zinc in sewage sludge before and after the extraction,it is found that the extracted zinc mainly comes from the acid soluble,reducible and oxidizable fractions.

Anti-inflammation Activities of Essential Oil and Anti-microbial Activities Of Ethanol Extraction from China's Rosemary

Rosemary(Rosmarius officinalis L.), an endemic plant species in south region of China, is traditionally used as a spice. In this research, the anti-inflammatory activities of essential oil and the antibacterial activities of ethanol extraction were determined, respectively. Results showed that based on the GC-MS analysis there were 35 kinds of active ingredients in the essential oil in totally, mainly including D-limonene(24.158 ml/L), α-Pinene(23.325 ml/L), Camphor(9.855 ml/L),Camphene(7.076 ml/L), Verbenone(6.685 ml/L), Borneol(5.580 ml/L), etc. The LCUV determination indicated that the main components in the ethanol extractionwere rosmarinic acid(3 910 mg/kg) and carnosic acid(2 970 mg/kg). By mice peritoneal macrophage phagocytosis of chicken erythrocytes experiment, the essential oil of rosemary was shown having a significant role in anti-inflammation. And the ethanol extraction had broad-spectrum antibacterial effects, but had no effect on mold by the agar diffusion method of 8 bacteria. As a result, both rosemary essential oil and ethanol extraction had good potential medicinal values.

Content Feature Extraction-based Hybrid Recommendation for Mobile Application Services

The number of mobile application services is showing an explosive growth trend,which makes it difficult for users to determine which ones are of interest.Especially,the new mobile application services are emerge continuously,most of them have not be rated when they need to be recommended to users.This is the typical problem of cold start in the field of collaborative filtering recommendation.This problem may makes it difficult for users to locate and acquire the services that they actually want,and the accuracy and novelty of service recommendations are also difficult to satisfy users.To solve this problem,a hybrid recommendation method for mobile application services based on content feature extraction is proposed in this paper.First,the proposed method in this paper extracts service content features through Natural Language Processing technologies such as word segmentation,part-of-speech tagging,and dependency parsing.It improves the accuracy of describing service attributes and the rationality of the method of calculating service similarity.Then,a language representation model called Bidirectional Encoder Representation from Transformers(BERT)is used to vectorize the content feature text,and an improved weighted word mover’s distance algorithm based on Term Frequency-Inverse Document Frequency(TFIDF-WMD)is used to calculate the similarity of mobile application services.Finally,the recommendation process is completed by combining the item-based collaborative filtering recommendation algorithm.The experimental results show that by using the proposed hybrid recommendation method presented in this paper,the cold start problem is alleviated to a certain extent,and the accuracy of the recommendation result has been significantly improved.

Performance comparison of different microbial DNA extraction methods on bird feces

Background:As an important player during food digestion,gut microbiota has attracted much attention in diet adaptation studies in birds.Microbiota extracted from feces has been widely used as a proxy for gut microbiota.Although several methods have been developed for microbial DNA extraction,their performances in the bird feces have not been systematacially evaluated yet.Methods:In this study,we applied three DNA extraction methods(Qiagen,MoBio and Bead)to extract DNA from feces of three avian dietary guilds(granivore,omnivore and carnivore),sequenced V4 region of 16S rRNA gene for each extract and evaluated the performances of DNA yield,DNA integrity,microbial composition,cell lysis capacity and alpha diversity for the three methods on each dietary guild.Results:Bead method was the best on the performance of both DNA yield and DNA integrity regardless of dietary guild.In granivore,microbial relative abundance at both species and phylum levels,alpha diversity and cell lysis capacity were comparable among all methods.In omnivore,Qiagen had the best performance on alpha diversity,fol-lowed by Bead and MoBio.There were small variations on microbial relative abundance at both species and phylum levels among different extraction methods.MoBio exhibited the best performance on cell lysis capacity.In carnivore,considerable variations were found on microbial relative abundance at both species and phylum levels.Qiagen had the best performance on alpha diversity,followed by MoBio and Bead.MoBio had the highest cell lysis capacity.Conclusions:DNA yield and integrity have no obvious impact on microbial composition,alpha diversity or cell lysis capacity.The microbiota results(e.g.,microbial composition,cell lysis capacity,alpha diversity)obtained from differ-ent methods are comparable in granivorous avian species but not in omnivorous or carnivorous birds.Either method could be used in granivore microbiota studies.For omnivores and carnivores,we recommend Qiagen method when the research purpose is microbial diversity and MoBio when gram-positive bacteria is the research target.

A feasible and effective solution-processed PCBM electron extraction layer enabling the high VOC and efficient Cu_(2)ZnSn(S, Se)_(4) devices

Photo-generated carrier recombination loss at the CZTSSe/Cd S front interface is a key issue to the opencircuit voltage(V_(OC)) deficit of Cu_(2)ZnSnS_(x)Se_(4-x)(CZTSSe) solar cells. Here, by the aid of an easy-handling spin-coating method, a thin PCBM([6,6]-phenyl-C61-butyric acid methyl ester) layer as an electron extraction layer has been introduced on the top of CdS buffer layer to modify CZTSSe/CdS/ZnO-ITO(In_(2)O_(3):Sn) interfacial properties. Based on Sn^(4+)/DMSO(dimethyl sulfoxide) solution system, a totalarea efficiency of 12.87% with a VOC of 529 m V has been achieved. A comprehensive investigation on the influence of PCBM layer on carrier extraction, transportation and recombination processes has been carried out. It is found that the PCBM layer can smooth over the Cd S film roughness, thus beneficial for a dense and flat window layer. Furthermore, this CZTSSe/Cd S/PCBM heterostructure can accelerate carrier separation and extraction and block holes from the front interface as well, which is mainly ascribed to the downward band bending of the absorber and a widened space charge region. Our work provides a feasible way to improve the front interfacial property and the cell performance of CZTSSe solar cells by the aid of organic interfacial materials.

Evaluation of extraction protocols for anti-diabetic phytochemical substances from medicinal plants

AIM To examine the efficacy of three extraction techniques: Soxhlet-extraction(SE), cold-maceration(CM) and microwave-assisted-extraction(MAE) using 80% methanol as solvent. METHODS The study was performed on each of 50 g of Vernonia amygdalina(VA) and Occimum gratissimum(OG) leaves respectively. The percentage yield, duration of extraction, volume of solvent used, qualitative and quantitative phytoconstituents present was compared. The biological activities(hypoglycemic effect) were investigated using albino wistar rat model of diabetes mellitus(n = 36) with a combined dose(1:1) of the two plants leaf extracts(250 mg/kg b.w.) from the three methods. Theextracts were administered orally, once daily for 21 d.RESULTS In this report, the percentage VA extract yield from MAE was highest(20.9% ± 1.05%) within 39 min using 250 m L of solvent, when compared to the CM(14.35% ± 0.28%) within 4320 min using 900 m L of solvent and SE(15.75% ± 0.71%) within 265 min using 500 m L of solvent. The percentage differences in OG extract yield between: MAE vs SE was 41.05%; MAE vs CM was 46.81% and SE vs CM was 9.77%. The qualitative chemical analysis of the two plants showed no difference in the various phytoconstituents tested, but differs quantitatively in the amount of the individual phytoconstituents, as MAE had significantly high yield(P > 0.05) on phenolics, saponins and tannins. SE technique gave significantly high yield(P > 0.05) on alkaloid, while CM gave significant high yield on flavonoids. The extracts from CM exhibited a significantly(P > 0.05) better hypoglycemic activity within the first 14-d of treatment(43.3% ± 3.62%) when compared to MAE(36.5% ± 0.08%) and SE methods(33.3% ± 1.60%). However, the percentage hypoglycemic activity, 21 d post-treatment with 250 mg/kg b.w. extract from MAE was 72.6% ± 1.03% and it was more comparable to 10 mg/kg b.w. glibenclamide treated group(75.0% ± 0.73%), unlike the SE(69.5% ± 0.71%) and CM(69.1% ± 1.03%). CONCLUSION CM technique produces extract with better hypoglycemic activity, whereas; MAE is a better option for high yield of phytoconstituents using less solvent within a short time.

Phytochemicals Screening, Phenolic Estimation and Evaluation for Anti-Oxidant, Anti-Inflammatory and Anti-Microbial Activities of Sequentially Soxhlet Extracted Coconut Testa

Background: In many coconut industries, the outer layer of thin brown skin of coconut kernel known as testa is peeled out as a byproduct. Despite the testa is rich in fat and plenty of polyphenolic compounds, it has been underutilized either as animal feed, serving as raw materials for bio-diesel production or discarded directly. Anticipating coconut testa (CT) as a natural source of multiple phyto-chemicals, its exploitation for the pharmacological activity or utilization as value added product is required which may reduce the disposal costs as well. Methods: Secondary metabolites from CT were extracted sequentially with different organic solvents based on polarity in the soxhlet apparatus followed by extraction with sterilized water. The crude dried extracts thus prepared were evaluated for qualitative screening of phytochemicals and quantitative estimation of total phenols, flavonoids and tannin content. Moreover, the antioxidant, anti-inflammatory and anti-microbial activities were also investigated. Results: Phytochemicals screening revealed the presence of polyphenolic compounds in methanolic fraction including phenols (822.60 ± 16.36 mg/g), flavonoids (103.30 ± 9.78 mg/g) and tannin (663.50 ± 19.26 mg/g), whereas non-phenolic compounds were present in other fractions. While methanolic fraction showed invariably the highest anti-oxidant activity in multiple assay methods, non-phenolic compounds in aqueous and chloroform fractions exhibited high anti-inflammatory activity. Antimicrobial activity was observed by both phenolic and non-phenolic compounds. Conclusion: The findings of the study reveal that CT is a rich source of various polyphenolic and non-phenolic natural antioxidants, anti-inflammatory and antimicrobial compounds. These findings are promising and form the basis to identify the number of active components and their characterization.

高效液相色谱法测定纺织品中四溴双酚S

建立了一种以磁固相萃取纺织品中四溴双酚S,再利用高效液相色谱分析的方法。试验以Fe_(3)O_(4)-GO磁性材料为磁性固相吸附剂,并采用正交试验对萃取过程中的萃取剂用量、温度和时间等因素进行优化,再以0.1 mmol甲酸-乙腈(V∶V=30∶70)为流动相,采用Athena C18-WP柱(2.1 mm×100.0 mm,5.00μm)分离,检测波长214 nm,外标法定量。结果表明,在0.01~50.00 mg/L范围内四溴双酚S线性良好,相关系数R^(2)>0.999,加标回收率达到98.6%~101.5%,相对标准偏差(RSD)小于5.0%(n=6)。该方法高效、简单、灵敏,能够满足纺织品中四溴双酚S的检测。

Evaluation of yields and quality parameters of oils from Cornus wilsoniana fruit extracted by subcritical n-butane extraction and conventional methods

Cornus wilsoniana fruit oil is a very important woody oil and is the main raw material of biodiesel.In this study,the oil yield,physicochemical properties,fatty acid composition,rheological properties,thermal stability,and Fourier transform infrared(FTIR)spectra of C.wilsoniana fruit oil obtained by subcritical n-butane extraction(SBE)and conventional methods such as pressing extraction(PE)and Soxhlet extraction(SE)were determined to study the influence of different extraction methods on the quality and yield of C.wilsoniana fruit oil.The oil yield of SBE(19.47%)was higher than that of PE(9.93%)but slightly lower than that of SE(21.08%).All of the extracted oils exhibited similar physicochemical properties,and the SBE oil was richer in polyunsaturated fatty acids(PUFA)than that of the PE oil,with an approximate 1:2 ratio of total saturated fatty acids against unsaturated fatty acids.The results of rheological behavior and thermal stability showed that all extracted oils had Newtonian flow characteristics,wherein the SBE oil exhibited lower viscosity and higher thermal stability.Furthermore,scanning electron microscopy(SEM)images of the surface topography indicated that different oil extraction methods will affect the residual oil content of the C.wilsoniana fruit powder.Compared with PE,the pores on the surface of the C.wilsoniana fruit powder after oil extraction were clearly visible,indicating that the driving force of SBE for oil extraction is stronger than that of PE.Based on the above results,it is implied that SBE is the best of the three methods for extracting C.wilsoniana fruit oil and can be potentially applied to extract other edible oils.

Curcuma′s extraction attenuates propranolol-induced psoriasis like in mice by inhibition of keratin,proliferating cell nuclear antigen and Toll-like receptor expression

OBJECTIVE Recently,some reports show that curcuma has a good curative effect on the treatment of psoriasis;while curcuma has many components,so the present study was designed to investigate the effective parts of curcuma on treatment of psoriasis,in addition,we will explore the mechanisms of curcuma therapeutic effect.METHODS First,we observed that curcuma′s extractions effect on mitosis of mouse vaginal epithelial cells;then psoriasis like was induced by wiping imiquimod in the surface of the binaural dorsal skin of the animal,the score of skin damage was measured on days 7 and 14;in order to explore the mechanisms of curcuma’s extractions on psoriasis,immune factors expression(CK14,CK16,CK17,PCNA,TLR-2,TLR-4,TLR-9)was determined in propranolol induced psoriasis like.RESULTS Curcuma′s extraction prohibited the mitosis of mouse vaginal epithelial cells;curcuma’s extractions produced a significant and dose dependent inhibition of psoriasis during the entire duration of the study in imiquimod induced psoriasis like;and the expression of immune factors(CK14,CK16,CK17,PCNA,TLR-2,TLR-4,TLR-9)was also found to be less in the curcuma′s extraction treated group as compared to control.CONCLUSION We believe that the curative effectof curcuma’sextraction may due to its inhibition effect on the expression of immune factors.Our results contribute towards validation of curcuma in the treatment of psoriasis and other joint disorders.

A Semi-automatic Method Based on Statistic for Mandarin Semantic Structures Extraction in Specific Domains

This paper proposed a new method of semi-automatic extraction for semantic structures from unlabelled corpora in specific domains. The approach is statistical in nature. The extracted structures can be used for shallow parsing and semantic labeling. By iteratively extracting new words and clustering words, we get an inital semantic lexicon that groups words of the same semantic meaning together as a class. After that, a bootstrapping algorithm is adopted to extract semantic structures. Then the semantic structures are used to extract new

Extraction of Partial Discharge Acoustic Signal by Wavelet Transform with Teager′s Energy Operator

To develop a measurement system for monitoring partial discharge （PD） without the effect of external interferences,an algorithm of PD signal extraction based on wavelet transform with Teager＇s energy operators was presented. Acoustic signal generated by PD was selected to remove excessive interfering signals and electromagnetic interferences. Acoustic signals were collected and decomposed into I0 levels by wavelet transform into approximation and detail components. “Daubechies 25” was proved to be the most suitable mother wavelet for the extraction of PD acoustic signals. Compared with conventional wavelet denoising method, Teager＇s energy operators were adopted to the PD signal reconstruction and the signal to noise ratio was in creased by 20%-25% inthe experiment,without lost in energy and pulse amplitude.