An improved method of using a selective spatial-domain mask to reduce speckle noise in digital holography is proposed.The sub-holograms are obtained from the original hologram filtered by the binary masks including a ...An improved method of using a selective spatial-domain mask to reduce speckle noise in digital holography is proposed.The sub-holograms are obtained from the original hologram filtered by the binary masks including a shifting aperture for being reconstructed. Normally, the speckle patterns of these sub-reconstructed images are different. The speckle intensity of the final reconstructed image is suppressed by averaging the favorable sub-reconstructed images which are selected based on the most optimal pixel intensity sub-range in the sub-holograms. Compared with the conventional spatial-domain mask method, the proposed method not only reduces the speckle noise more effectively with fewer sub-reconstructed images,but also reduces the redundant information used in the reconstruction process.展开更多
基金Project supported by Guangdong Provincial Science and Technology Plan Project of China(Grant Nos.2015B010114007 and 2014B050505020)
文摘An improved method of using a selective spatial-domain mask to reduce speckle noise in digital holography is proposed.The sub-holograms are obtained from the original hologram filtered by the binary masks including a shifting aperture for being reconstructed. Normally, the speckle patterns of these sub-reconstructed images are different. The speckle intensity of the final reconstructed image is suppressed by averaging the favorable sub-reconstructed images which are selected based on the most optimal pixel intensity sub-range in the sub-holograms. Compared with the conventional spatial-domain mask method, the proposed method not only reduces the speckle noise more effectively with fewer sub-reconstructed images,but also reduces the redundant information used in the reconstruction process.
文摘超分辨结构光照明显微成像技术(super-resolution structured illumination microscopy,SR-SIM)具有时间分辨率高、光漂白和光毒性低和对荧光探针的要求少等优点,适用于活细胞的长时程超分辨成像.采用二维晶格结构光作为照明光,可以实现更快的成像速度和更低的光毒性,但同时也增加了系统的复杂性.为了解决此问题,本文提出了一种基于数字微镜器件的快速超分辨晶格结构光照明显微成像方法(digital micromirror device-based lattice SIM,DMD-Lattice-SIM),通过同步分时触发DMD和sCMOS相机的方式实现二维正交晶格结构光的产生,且只需要采集5幅相移原始图像即可重构出超分辨图像,相比于传统SR-SIM需要9幅相移原始图像的方法,图像采集时间减少了约44.4%.同时,在基于空域和频域联合的SIM重构算法(joint space and frequency reconstruction method-SIM,JSFR-SIM)的基础上,本文还发展了用于Lattice-SIM的JSFR超分辨图像重构方法(Lattice-JSFR-SIM),先在频域对原始图像进行预滤波处理;然后,在空域对滤波后的图像进行超分辨重构处理.与传统频域图像重构处理对比,该方法在512×512像素数的成像视场下重构时间减少了约55.6%,对于实现活细胞实时超分辨成像具有重要意义和应用价值.