[目的]研究鞘内注射氟代柠檬酸（fluorocitrate FC）和米诺四环素（minocychine MC）对背根节慢性压迫模型（the experi ment model for chronic compression of dorsal root ganglion,CCD）大鼠脊髓背角星形胶质细胞活化增殖的影响。[...[目的]研究鞘内注射氟代柠檬酸（fluorocitrate FC）和米诺四环素（minocychine MC）对背根节慢性压迫模型（the experi ment model for chronic compression of dorsal root ganglion,CCD）大鼠脊髓背角星形胶质细胞活化增殖的影响。[方法]采用大鼠脊髓背根节慢性压迫实验模型,96只鞘内置管SD大鼠分为第7天组（A组）和第14天组（B组）,每组随机分为六个亚组组,每小组（n=8）包括：假手术（sham）组,CCD组,溶剂磷酸盐缓冲生理盐水（PBS）组（0.01 mmol/L PBS 20μLi.t.）,FC组（1μmol/L FC20μLi.t.）,MC组（5 g/L MC 20μLi.t.）,MC＋FC组（1μmol/L FC和5 g/L MC混合液20μLi.t.）。术后每天给药一次,分别于术后d7和d14运用免疫荧光化学方法观察脊髓背角胶原纤维酸性蛋白（GFAP）的表达。[结果]脊髓背角GFAP阳性表达CCD、PBS、FC组在Ⅰ-Ⅳ层都表达明显,在数目上没有差异性;而MC、MC＋FC组主要集中在Ⅰ-Ⅱ层,Ⅲ、Ⅳ层在数目上有明显减少。CCD组和PBS组胞体比其余各组明显肥大。[结论]鞘内给药后,慢性神经痛大鼠脊髓背角星形胶质细胞GFAP表达减少,提示小胶质细胞抑制剂MC能很好的抑制星形胶质细胞的活化,小胶质细胞在星形胶质细胞活化的形成和维持中可能起重要作用。展开更多
Valproic acid has been shown to exert neuroprotective effects and promote neurite outgrowth in several peripheral nerve injury models. However, whether valproic acid can exert its beneficial effect on neurons after br...Valproic acid has been shown to exert neuroprotective effects and promote neurite outgrowth in several peripheral nerve injury models. However, whether valproic acid can exert its beneficial effect on neurons after brachial plexus avulsion injury is currently unknown. In this study, brachial plexus root avulsion models, established in Wistar rats, were administered daily with valproic acid dis-solved in drinking water （300 mg/kg） or normal water. On days 1, 2, 3, 7, 14 and 28 after avulsion injury, tissues of the C 5-T 1 spinal cord segments of the avulsion injured side were harvested to in-vestigate the expression of Bcl-2, c-Jun and growth associated protein 43 by real-time PCR and western blot assay. Results showed that valproic acid significantly increased the expression of Bcl-2 and growth associated protein 43, and reduced the c-Jun expression after brachial plexus avulsion. Our findings indicate that valproic acid can protect neurons in the spinal cord and enhance neuronal regeneration fol owing brachial plexus root avulsion.展开更多
文摘[目的]研究鞘内注射氟代柠檬酸（fluorocitrate FC）和米诺四环素（minocychine MC）对背根节慢性压迫模型（the experi ment model for chronic compression of dorsal root ganglion,CCD）大鼠脊髓背角星形胶质细胞活化增殖的影响。[方法]采用大鼠脊髓背根节慢性压迫实验模型,96只鞘内置管SD大鼠分为第7天组（A组）和第14天组（B组）,每组随机分为六个亚组组,每小组（n=8）包括：假手术（sham）组,CCD组,溶剂磷酸盐缓冲生理盐水（PBS）组（0.01 mmol/L PBS 20μLi.t.）,FC组（1μmol/L FC20μLi.t.）,MC组（5 g/L MC 20μLi.t.）,MC＋FC组（1μmol/L FC和5 g/L MC混合液20μLi.t.）。术后每天给药一次,分别于术后d7和d14运用免疫荧光化学方法观察脊髓背角胶原纤维酸性蛋白（GFAP）的表达。[结果]脊髓背角GFAP阳性表达CCD、PBS、FC组在Ⅰ-Ⅳ层都表达明显,在数目上没有差异性;而MC、MC＋FC组主要集中在Ⅰ-Ⅱ层,Ⅲ、Ⅳ层在数目上有明显减少。CCD组和PBS组胞体比其余各组明显肥大。[结论]鞘内给药后,慢性神经痛大鼠脊髓背角星形胶质细胞GFAP表达减少,提示小胶质细胞抑制剂MC能很好的抑制星形胶质细胞的活化,小胶质细胞在星形胶质细胞活化的形成和维持中可能起重要作用。
基金supported by Graduated Innovation Fund of Jilin University,No.20121115the National Natural Science Foundation of China,No.30872626+1 种基金Key Projects of Clinical Sciences by the Ministry of Health,No.439the Research Fund for the Doctoral Program of Higher Education,No.20070183143
文摘Valproic acid has been shown to exert neuroprotective effects and promote neurite outgrowth in several peripheral nerve injury models. However, whether valproic acid can exert its beneficial effect on neurons after brachial plexus avulsion injury is currently unknown. In this study, brachial plexus root avulsion models, established in Wistar rats, were administered daily with valproic acid dis-solved in drinking water （300 mg/kg） or normal water. On days 1, 2, 3, 7, 14 and 28 after avulsion injury, tissues of the C 5-T 1 spinal cord segments of the avulsion injured side were harvested to in-vestigate the expression of Bcl-2, c-Jun and growth associated protein 43 by real-time PCR and western blot assay. Results showed that valproic acid significantly increased the expression of Bcl-2 and growth associated protein 43, and reduced the c-Jun expression after brachial plexus avulsion. Our findings indicate that valproic acid can protect neurons in the spinal cord and enhance neuronal regeneration fol owing brachial plexus root avulsion.
