Most probable number （MPN） and colony-forming unit （CFU） estimates of fecal indicator bacteria （FIB） concentration are common measures of water quality in aquatic environments. Thus, FIB intensively monitored in...Most probable number （MPN） and colony-forming unit （CFU） estimates of fecal indicator bacteria （FIB） concentration are common measures of water quality in aquatic environments. Thus, FIB intensively monitored in Yeongsan Watershed in an attempt to compare two different methods and to develop a statistical model to convert from CFU to MPN estimates or vice versa. As a result, the significant difference was found in the MPN and CFU estimates. The enumerated Escherichia coli concentrations in MPN are greater than those in CFU, except for the measurement in winter. Especially in fall, E. coli concentrations in MPN are one order of magnitude greater than that in CFU. Contrarily, enterococci bacteria in MPN are lower than those in CFU. However, in general, a strongly positive relationship are found between MPN and CFU estimates. Therefore, the statistical models were developed, and showed the reasonable converting FIB concentrations from CFU estimates to MPN estimates. We expect this study will provide preliminary information towards future research on whether different analysis methods may result in different water quality standard violation frequencies for the same water sample.展开更多
目的:观察健中益肾汤联合针刺治疗脾肾两虚型重症监护病区获得性肌无力(Intensive Care Unit Acquired Myasthenia,ICUAW)的临床效果。方法:选取2020年1月-2022年1月龙岩市中医院收治的ICUAW患者90例,采用随机数字表法分为A组、B组、C组...目的:观察健中益肾汤联合针刺治疗脾肾两虚型重症监护病区获得性肌无力(Intensive Care Unit Acquired Myasthenia,ICUAW)的临床效果。方法:选取2020年1月-2022年1月龙岩市中医院收治的ICUAW患者90例,采用随机数字表法分为A组、B组、C组。A组采用西医常规治疗,B组在A组常规治疗的基础上联合应用健中益肾汤治疗,C组在西医常规治疗的基础上采用健中益肾汤联合针刺治疗。观察三组患者治疗前后中医证候积分、英国医学研究理事会量表(Medical Research Council,MRC)评分、改良Barthel指数量表(Modified Barthel Index,MBI)评分、机械通气时长、重症监护病区(ICU)住院天数,以及肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白细胞介素-6(Interleukin 6,IL-6)水平。结果:三组患者治疗前中医证候积分、MRC评分、MBI评分及TNF-α、IL-6水平的差异无统计学意义(P>0.05)。治疗后,三组患者的中医证候积分、MRC评分、MBI评分、机械通气时长、ICU住院天数及TNF-α、IL-6水平与治疗前比较均改善(P<0.05),且C组的改善较A组、B组显著(P<0.05)。C组机械通气时长、ICU住院天数明显少于A组、B组(P<0.05)。结论:健中益肾汤联合针刺治疗脾肾两虚型ICUAW效果显著,可显著改善患者的临床症状,促进患者预后,其机制可能与减轻炎症反应有关。展开更多
The protective effect of a kind of purified polysaccharides extracted from Radix of Phytolacca acinosa Roxb,with a molecular weight of 10 KDa,on hematopoiesis was investigated.Average survival time of mice treated wit...The protective effect of a kind of purified polysaccharides extracted from Radix of Phytolacca acinosa Roxb,with a molecular weight of 10 KDa,on hematopoiesis was investigated.Average survival time of mice treated with cyclophosphamide (CY) 300 mg/kg once alone was 13.3 ± 7.2d(n=7) however,average survival time of mice treated with CY 300 mg/kg in com-bination with PAP-1 10 mg/kg,3 times/wk was 36.7± 16.4d(n=7,P<0.01).PAP-1,ip had benefi-cial effect on the recovery of the CY induced decrease of peripheral leukocyte number,and the nu-cleated bone marrow cell(BMC)number and[3￣H]TdR uptaken by BMC induced by rmGM-CSF in S180 bearing mice treated with CY,In mice,after the first ip treatment with CY 100 mg/kg on d7,the peripheral leukocyte number decreased on d9 and recovered to normal level about d13 to d15. Such recovery was accelerated by administrating PAP-1,10mg/kg, 3 times/wk.A significant in-crease of the activity to form colony in spleen(colony-forming unit in spleen, CFU-S_8, CUF-S12) in mice irradiated with 550 rad 6O￣Co γ-rays and an enhancement of proliferative response of BMC to rmGM-CSF treated with PAP-1,10mg/kg,3 times/wk, ip were observed.After PAP-1,10 mg/kg,ip once,a significant increase in the number of peripheral blood leukocytes and a rise in the serum of colony stimulating factor(CSF) were also confirmed.The types of CSF in serum were M-CSF and other hematopoietic growth factors,which were confirmed by using McAb of IL-3, GM-CSF and PcAb of M-CSF. These beneficial effects of PAP-1 on hematopoiesis may be related to its activityinducing CSFs and other hematopoietic growth factors and warrant further evaluation of its use-fulness.展开更多
基金supported by the Korean Ministry of Environment as "The Eco-technopia 21 Project" (No. 019-071-053)
文摘Most probable number （MPN） and colony-forming unit （CFU） estimates of fecal indicator bacteria （FIB） concentration are common measures of water quality in aquatic environments. Thus, FIB intensively monitored in Yeongsan Watershed in an attempt to compare two different methods and to develop a statistical model to convert from CFU to MPN estimates or vice versa. As a result, the significant difference was found in the MPN and CFU estimates. The enumerated Escherichia coli concentrations in MPN are greater than those in CFU, except for the measurement in winter. Especially in fall, E. coli concentrations in MPN are one order of magnitude greater than that in CFU. Contrarily, enterococci bacteria in MPN are lower than those in CFU. However, in general, a strongly positive relationship are found between MPN and CFU estimates. Therefore, the statistical models were developed, and showed the reasonable converting FIB concentrations from CFU estimates to MPN estimates. We expect this study will provide preliminary information towards future research on whether different analysis methods may result in different water quality standard violation frequencies for the same water sample.
文摘目的:观察健中益肾汤联合针刺治疗脾肾两虚型重症监护病区获得性肌无力(Intensive Care Unit Acquired Myasthenia,ICUAW)的临床效果。方法:选取2020年1月-2022年1月龙岩市中医院收治的ICUAW患者90例,采用随机数字表法分为A组、B组、C组。A组采用西医常规治疗,B组在A组常规治疗的基础上联合应用健中益肾汤治疗,C组在西医常规治疗的基础上采用健中益肾汤联合针刺治疗。观察三组患者治疗前后中医证候积分、英国医学研究理事会量表(Medical Research Council,MRC)评分、改良Barthel指数量表(Modified Barthel Index,MBI)评分、机械通气时长、重症监护病区(ICU)住院天数,以及肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白细胞介素-6(Interleukin 6,IL-6)水平。结果:三组患者治疗前中医证候积分、MRC评分、MBI评分及TNF-α、IL-6水平的差异无统计学意义(P>0.05)。治疗后,三组患者的中医证候积分、MRC评分、MBI评分、机械通气时长、ICU住院天数及TNF-α、IL-6水平与治疗前比较均改善(P<0.05),且C组的改善较A组、B组显著(P<0.05)。C组机械通气时长、ICU住院天数明显少于A组、B组(P<0.05)。结论:健中益肾汤联合针刺治疗脾肾两虚型ICUAW效果显著,可显著改善患者的临床症状,促进患者预后,其机制可能与减轻炎症反应有关。
文摘The protective effect of a kind of purified polysaccharides extracted from Radix of Phytolacca acinosa Roxb,with a molecular weight of 10 KDa,on hematopoiesis was investigated.Average survival time of mice treated with cyclophosphamide (CY) 300 mg/kg once alone was 13.3 ± 7.2d(n=7) however,average survival time of mice treated with CY 300 mg/kg in com-bination with PAP-1 10 mg/kg,3 times/wk was 36.7± 16.4d(n=7,P<0.01).PAP-1,ip had benefi-cial effect on the recovery of the CY induced decrease of peripheral leukocyte number,and the nu-cleated bone marrow cell(BMC)number and[3￣H]TdR uptaken by BMC induced by rmGM-CSF in S180 bearing mice treated with CY,In mice,after the first ip treatment with CY 100 mg/kg on d7,the peripheral leukocyte number decreased on d9 and recovered to normal level about d13 to d15. Such recovery was accelerated by administrating PAP-1,10mg/kg, 3 times/wk.A significant in-crease of the activity to form colony in spleen(colony-forming unit in spleen, CFU-S_8, CUF-S12) in mice irradiated with 550 rad 6O￣Co γ-rays and an enhancement of proliferative response of BMC to rmGM-CSF treated with PAP-1,10mg/kg,3 times/wk, ip were observed.After PAP-1,10 mg/kg,ip once,a significant increase in the number of peripheral blood leukocytes and a rise in the serum of colony stimulating factor(CSF) were also confirmed.The types of CSF in serum were M-CSF and other hematopoietic growth factors,which were confirmed by using McAb of IL-3, GM-CSF and PcAb of M-CSF. These beneficial effects of PAP-1 on hematopoiesis may be related to its activityinducing CSFs and other hematopoietic growth factors and warrant further evaluation of its use-fulness.
