针刺结合康复训练对免疫抑制大鼠脾脏指数及血清IL-2的影响

目的：观察针刺与康复训练相结合对免疫抑制大鼠模型脾脏指数及血清IL-2的影响。方法：将32只健康SD大鼠（雄性）体重200~250g,完全随机分为4组：模型组、康复治疗组、针康组、针刺治疗组,每组8只。除模型组外,其他各组分别给予针刺或康复治疗。每日1次,治疗10天后,取大鼠脾脏及血清。计算脾脏指数,用放射免疫法检测IL-2值。结果：①与模型组比较,康复治疗组和针康组脾脏指数有明显提高,并有显著性差异（P〈0.05）。②与针刺组比较,康复治疗组和针康组脾脏指数有明显提高,并有显著性差异（P〈0.05）。③与模型组比较,康复治疗组、针刺治疗组血清IL-2含量升高并有显著性差异（P〈0.05）。与模型组比较针康组血清IL-2含量升高并有非常显著性差异（P〈0.01）。结论：①针刺结合康复训练能提高免疫抑制大鼠脾脏指数。②针刺与康复训练均能提高免疫抑制大鼠血清IL-2含量,二者结合效果更明显。

Effect of Yupingfeng Polysaccharide on Serum IL-2 and IFN-γ Levels in Immunosuppressive Mice

[Objective] The paper was to discuss regulation and protection effect of Yupingfeng polysaccharide （YPF-P） on immunosuppressive mice. [Method] Taking immunosuppressive mice induced by 80 mg/kg cyclophosphamide as test animal model, effects of different doses （100,200 and 400 mg/kg） of YPF-P on spleen index, serum IL-2 and IFN-γ levels of mice were studied. [ Result] High dose of YPF-P （400 mg/kg） could improve the spleen index of immunosuppressive mice （P 〈0.05）. YPF-P could enhance serum IL-2 and IFN-γ levels of immunosuppressive mice, and the effects in middle-dose group and low-dose group were better. YPF-P could significantly increase serum IL-2 level of normal mice （ P 〈 0.01 ）, but it had no significant effect on serum IFN-γ level of normal mice. [ Conclusion] The research provides an experimental basis for clinical application of YPF-P.

Effects of Raspberry Root decoction on cytokine level and thymus and spleen index in S180 model mice

Objective: To observe the effects of RRD on serum levels of cytokines interleukin-2 (IL-2), interleukin-10 (IL-10) and thymus and spleen index in S180 mice, and to explore the mechanism of tumor inhibition by RRD. Methods: Fifty Kunming healthy mice, half male and half female, were randomly divided into five groups: normal control group, model control group, cyclophosphamide group (CTX group), red raspberry group (RRD group) and combined administration of red raspberry and cyclophosphamide group (RRD + CTX group), with 10 mice in each group only. The other 40 mice were injected with 0.2 mLS180 tumor suspension at the right axilla to make the model experiment, except 10 mice in the normal control group. The next day, the normal control group and model control group were given intragastric administration of 0.02 mL/g/d saline, CTX group was given intragastric administration of 0.4 mL/20 g/d saline and 20 mg/kg/d CTX, RRD group was given intragastric administration of 0.4 mL/20 g/d RRD, RRD+CTX group was given intragastric administration of 0.4 mL/20 g/d RRD and 20 mg/kg/d CTX for 10 d, once a day. Serum levels of IL-2 and IL-10 were measured by ELISA, and thymus and spleen indexes were measured. Results: Red raspberry rhizome decoction could increase serum IL-2 level (P < 0.05), decrease IL-10 level (P < 0.05), increase thymus index (P < 0.05) and decrease spleen index (P < 0.05) in S180 mice. Conclusion:The anti-tumor effect of the water decoction of red raspberry rhizome may be related to the regulation of immune suppression and the improvement of immune organ function of the tumor-bearing organism.

Correlation between IL-33/sST2 signaling pathway and patients with essential hypertensive left ventricular hypertrophy

Objective:To detect the levels of interleukin-33(IL-33)and soluble ST2(sST2)in peripheral blood of patients with essential hypertensive left ventricular hypertrophy,and to discusstheir correlation with patients with essential hypertensive left ventricular hypertrophy was further discussed.Methods:A total of 220 patients with essential hypertension treated in the Department of Cardiovascular Medicine of the First Affiliated Hospital of Bengbu Medical College were enrolled as the experimental group.According to left ventricular mass index(LVMI),patients with essential hypertension were divided into the non-left ventricular hypertrophy group(NLVH,n=108 cases)and the left ventricular hypertrophy group(LVH,n=112 cases).We used ELISA to detect the serum levels of IL-33 and sST2,the expression levels of IL-33 in peripheral blood lymphocytes of the NLVH group and the LVH group(60 cases each)were detected by Western blot,and the relationship between IL-33 and LVMI,a marker of left ventricular hypertrophic condition,was analyzed by Pearson.The relationship between IL-33,sST2 and left ventricular hypertrophy in essential hypertension was studied.Results:Compared with the NLVH group,the expression levels of IL-33 and sST2 in the LVH group were significantly increased.The results of Western blot showed that the expression level of IL-33 in the LVH group(1.07±0.08)was higher than that in the NLVH group(0.63±0.05)(P<0.05).Pearson correlation analysis showed that IL-33 was positively correlated with LVMI,sST2 was positively correlated with LVMI.Conclusion:The levels of IL-33 and sST2 in serum and the expression levels of IL-33 protein in peripheral blood lymphocytes are significantly increased in patients with hypertensive left ventricular hypertrophy,and the occurrence and development of essential hypertensive left ventricular hypertrophy may be related to IL-33 and sST2.

健脾养胃膏联合穴位埋线对脾虚湿蕴证肥胖型2型糖尿病患者体格指标及糖脂代谢水平的影响

目的 探讨健脾养胃膏联合穴位埋线对脾虚湿蕴证肥胖型2型糖尿病患者体格指标及糖脂代谢水平的影响。方法 选取2023年7月—2024年1月宜兴市中医医院收治的88例脾虚湿蕴证肥胖型2型糖尿病患者为研究对象,按照不同治疗方法分为对照组和观察组,各44例。对照组给予健脾养胃膏治疗,观察组在此基础上联合穴位埋线。比较两组体格指标及糖脂代谢指标。结果 治疗后,观察组体重指数、腰围、臀围、空腹血糖、餐后2 h血糖、糖化血红蛋白、总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平均低于对照组,而高密度脂蛋白胆固醇水平高于对照组,差异有统计学意义(P均<0.05)。结论 健脾养胃膏联合穴位埋线治疗能有效改善脾虚湿蕴证肥胖型2型糖尿病患者的体格指标和糖脂代谢水平。

三种治法方药对肝癌H_(22)荷瘤小鼠免疫功能影响的比较研究

目的:观察比较活血化瘀方、清热祛湿方和养阴柔肝方三种不同治法中药复方对H22荷瘤小鼠免疫功能的影响。方法:采用皮下接种肝癌H22细胞的方法建立荷瘤小鼠模型,分别用上述三种中药复方水煎液灌胃处理11d,称量各组脾脏重量,计算脾指数;应用双抗体夹心ABC-ELISA法检测荷瘤小鼠血清IL-2、TNF-α含量的变化。结果:活血化瘀、清热祛湿、养阴柔肝方对H22小鼠脾指数无显著影响(P>0.05);与正常对照组相比,模型对照组血清IL-2的含量显著降低(P<0.05),各用药组与模型对照比较显著升高(P<0.05),三个用药组之间两两比较,也具有显著性差异(P<0.05);与正常对照组相比,各组血清TNF-α的含量均显著升高(P<0.05),与模型对照比较,各用药组显著降低(P<0.05)。结论:三种治法均可有效提高移植性H22荷瘤小鼠的IL-2水平,提高小鼠的免疫力,从而对机体起到保护作用,这可能是其有效抗肝癌的疗效机制之一;三种治法(尤其是清热祛湿组)具有抑制荷瘤鼠机体TNF-α水平异常升高的作用,使其维持在一定水平而发挥抗肿瘤、调节机体免疫功能等积极作用。

黄连解毒汤对2型糖尿病大鼠脾脏T细胞亚群的干预

目的:研究黄连解毒汤对2型糖尿病大鼠免疫状态的影响。方法:采用STZ腹腔注射联合高糖高脂饲料喂养Wistar大鼠,建立2型糖尿病模型。治疗6周后,称重脾脏并计算脾脏指数;免疫组化方法检测脾脏CD+4、CD+8T细胞亚群的表达,计算CD+4/CD+8比值。结果:黄连解毒汤治疗组大鼠体重显著下降,脾脏指数明显升高,脾脏CD+4、CD+8的表达显著增加(P<0.01),CD+4/CD+8比值显著升高(P<0.01),且明显优于二甲双胍对照组(P<0.01)。结论:黄连解毒汤可以有效控制2型糖尿病大鼠体重,对2型糖尿病引起的T细胞亚群异常有良好的调节作用。

清热利湿健脾降糖方联合穴位埋线治疗肥胖型2型糖尿病湿热困脾证患者的疗效观察

目的探讨清热利湿健脾降糖方联合穴位埋线治疗肥胖型2型糖尿病(Type 2 diabetes,T2DM)湿热困脾证的临床价值。方法选取2020年1月—2021年10月期间空军军医大学第一附属医院收治的肥胖型T2DM湿热困脾证患者88例,采用随机数字表法分为对照组与试验组,每组各44例。两组患者均开展T2DM基础治疗,在此基础上,对照组给予盐酸二甲双胍治疗,试验组给予盐酸二甲双胍+中医特色疗法(清热利湿健脾降糖方、穴位埋线)。治疗12周后,观察比较两组患者治疗前后中医证候积分、血糖指标[空腹血糖(Fasting plasma glucose,FPG)、餐后2 h血糖(Blood glucose 2 h after meal,2 h PG)、糖化血红蛋白(Glycosylated hemoglobin,HbAlc)]、血脂指标[高密度脂蛋白(High density lipoprotein,HDL)、低密度脂蛋白(Low density lipoprotein,LDL)、甘油三酯(Triglyceride,TG)、总胆固醇(Total cholesterol,TC)]、肥胖指数[体质量指数(Body mass index,BMI)、腰臀比(Waist hip ratio,WHR)]、生活质量评分(Adjusted diabetes-specific quality of life scale,A-DQOL)变化及中医证候疗效、治疗安全性。结果(1)中医证候积分:治疗后两组患者中医证候积分均较治疗前降低,差异有统计学意义(P<0.05);且试验组中医证候积分均低于对照组,差异有统计学意义(P<0.05);(2)中医证候疗效:治疗后试验组中医证候总有效率92.86%(39/42)高于对照组64.29%(27/42),差异有统计学意义(P<0.05);(3)血糖水平:治疗后两组患者血糖FPG、2 h PG、HbAlc水平均较治疗前降低,差异有统计学意义(P<0.05);且试验组血糖FPG、2 h PG、HbAlc水平均低于对照组,差异有统计学意义(P<0.05);(4)血脂水平:治疗后两组患者血脂HDL-C、LDL-C、TG、TC水平均较治疗前改善,差异有统计学意义(P<0.05);且试验组血脂HDL-C、LDL-C、TG、TC水平改善均优于对照组,差异有统计学意义(P<0.05);(5)肥胖指数:治疗后两组患者肥胖指数BMI、WHR均较治疗前降低,差异有统计学意义(P<0.05);且试验组肥胖指数BMI、WHR均低于对照组,差异有统计学意义(P<0.05);(6)生活质量:治疗后两组患者生活质量A-DQOL评分均较治疗前降低,差异有统计学意义(P<0.05);且试验组生活质量A-DQOL评分均低于对照组,差异有统计学意义(P<0.05);(7)安全性:试验组不良反应发生率11.90%(5/42)与对照组9.52%(4/42)比较,差异无统计学意义(P>0.05)。结论清热利湿健脾降糖方联合穴位埋线治疗肥胖型T2DM湿热困脾证安全、有效,有利于控制血糖、血脂水平,改善患者肥胖指数及生活质量。

早发2型糖尿病中医证型与危险因素及生化指标的关联性研究

【目的】研究早发2型糖尿病(type 2 diabetes mellitus,T2DM)的中医证型分布规律,并探讨中医证型与危险因素及生化指标之间的关联性。【方法】收集130例早发T2DM患者的四诊资料,确定中医证型,采集患者的一般资料、危险因素及生化指标,运用SPSS 25.0统计软件分析早发T2DM的中医证型分布特点及其与一般资料、危险因素和生化指标的关系。【结果】共收集130例早发T2DM患者,男女比例为3.19∶1,男性发病率偏高。证型分布从高到低依次为湿热困脾证(30.77%)、阴虚热盛证(28.46%)、气阴两虚证(18.46%)、血络瘀阻证(11.54%)、阴阳两虚证(10.77%)。病程早期(≤1年)以湿热困脾证、阴虚热盛证较多,分别为36.49%、33.78%,后期(病程≥5年)以气阴两虚证、阴阳两虚证为主,分别为31.03%、27.59%。平均体质量指数(BMI)为(25.66±4.30)kg/m2,以湿热困脾证最高。主要发病危险因素为:糖尿病家族史、高脂饮食和缺乏运动,其占比均超过50%,其中高脂饮食和缺乏运动以湿热困脾证最多,高糖饮食以阴虚热盛证最多,空腹C肽及餐后1 h C肽以阴虚热盛证最低、湿热困脾证最高,甘油三酯(TG)以湿热困脾证最高,血尿素氮(BUN)、血肌酐(Scr)以阴阳两虚证最高,其次为气阴两虚证,预估肾小球滤过率(eGFR)以阴阳两虚证最低,其次为气阴两虚证。【结论】早发T2DM早期主要以湿热困脾证和阴虚热盛证的实证为主,随着病情进展,逐渐向气阴两虚和阴阳两虚的虚证转变。其中,湿热困脾证多存在高脂饮食和缺乏运动,并伴有体质量超重、高TG血症和胰岛素抵抗的临床问题;阴虚热盛证多存在高糖饮食,伴有血糖偏高和胰岛素缺乏;气阴两虚及阴阳两虚证患者多处于病程的后期,具有肾功能较差的临床问题。

Immunoregulatory Effects of Sanguis Draconis Flavones on Mouse Spleen Lymphocytes

[Objectives] This study aimed to study the effects of Sanguis Draconis flavones( SDF) on the immune regulation of mouse spleen lymphocytes. [Methods]A total of 60 BALB/c male mice were evenly and randomly divided into normal control group,model group,positive group,and high,middle and low-dose SDF groups. The mice in the model group were injected intraperitoneally with cyclophosphamide to establish mouse immunosuppressive model. The mice in the other groups were intragastrically administered at the respective doses,and the body weight was weighed once a week during the administration period. After 30 d of administration,the thymus and spleen indexes,the spleen lymphocyte proliferation ability,the NK cell activity,and the IL-2,IL-4 and INF-γ contents in the culture supernatant were measured. [Results]SDF could significantly increase the thymus and spleen indexes and improve the spleen lymphocyte proliferation ability and NK cell activity of the mice,as well as increasing the IL-2 and INF-γ contents and reducing the IL-4 content in spleen lymphocyte suspension culture. [Conclusions] SDF have good immunoregulatory effect.

Discovery of highly immunogenic spleen-resident FCGR3^(+)CD103^(+)cDC1s differentiated by IL-33-primed ST2^(+)basophils

Recombinant interleukin-33(IL-33)inhibits tumor growth,but the detailed immunological mechanism is still unknown.IL-33-mediated tumor suppression did not occur in Batf3^(−/−)mice,indicating that conventional type 1 dendritic cells(cDC1s)play a key role in IL-33-mediated antitumor immunity.A population of CD103^(+)cDC1s,which were barely detectable in the spleens of normal mice,increased significantly in the spleens of IL-33-treated mice.The newly emerged splenic CD103^(+)cDC1s were distinct from conventional splenic cDC1s based on their spleen residency,robust effector T-cell priming ability,and surface expression of FCGR3.DCs and DC precursors did not express Suppressor of Tumorigenicity 2(ST2).However,recombinant IL-33 induced spleen-resident FCGR3^(+)CD103^(+)cDC1s,which were found to be differentiated from DC precursors by bystander ST2+immune cells.Through immune cell fractionation and depletion assays,we found that IL-33-primed ST2^(+)basophils play a crucial role in the development of FCGR3^(+)CD103^(+)cDC1s by secreting IL-33-driven extrinsic factors.Recombinant GM-CSF also induced the population of CD103^(+)cDC1s,but the population neither expressed FCGR3 nor induced any discernable antitumor immunity.The population of FCGR3^(+)CD103^(+)cDC1s was also generated in vitro culture of Flt3L-mediated bone marrow-derived DCs(FL-BMDCs)when IL-33 was added in a pre-DC stage of culture.FL-BMDCs generated in the presence of IL-33(FL-33-DCs)offered more potent tumor immunotherapy than control Flt3L-BMDCs(FL-DCs).Human monocyte-derived DCs were also more immunogenic when exposed to IL-33-induced factors.Our findings suggest that recombinant IL-33 or an IL-33-mediated DC vaccine could be an attractive protocol for better tumor immunotherapy.

肉苁蓉多糖的促淋巴细胞增殖作用

目的研究肉苁蓉多糖(CDPS)对小鼠淋巴细胞增殖的影响。方法MTT法检测小鼠脾淋巴细胞的增殖。环磷酰胺(Cy)复制免疫功能低下的动物模型,分别测定正常及免疫低下动物脾脏、胸腺指数。胸腺细胞增殖法测定白细胞介素-2(IL-2)活性。结果CDPS对丝裂原(ConA及LPS)活化淋巴细胞及未活化正常细胞均有明显促增殖作用,并促进淋巴细胞IL-2的分泌。腹腔给药显示CDPS具明显提高正常及免疫低下小鼠的脾指数,对因Cy所致胸腺指数的降低也有显著的对抗作用。结论CDPS可显著促进小鼠脾淋巴细胞增殖,该作用可能与其促IL-2分泌有关。

加味青麟丸提高老龄大鼠免疫能力作用的研究

目的：观察加味青麟丸（JL）对老龄大鼠免疫能力的影响。方法：选用wistar大鼠120只，随机分为6组．即4月龄空白对照组（4C组）、14月龄空白对照组（14C组）、14月龄加味青麟丸组（14JL组）、24月龄空白对照组（24C组）、24月龄加味青麟丸组（24JL组）和14—24月龄加味青麟丸组（14～24JL组），每组各20只。雌雄各半。空白对照各组：在4月龄时先剖检4c组大鼠，用于对照。其余40只大鼠用普通饲料喂养，每晨灌喂与加味青麟丸等量的生理盐水。＂-3喂至14月龄时剖检14C组大鼠；同法，当喂至24月龄时剖检24C组大鼠。加味青麟丸各组：每晨灌喂加味青麟丸水溶液，其生药量按人与大鼠体表面积换算的等效剂量0．54．g／（kg·d），每周称1次体重。14JL组即从4月龄每晨灌喂加味青麟丸至14月龄；24JL组即从4月龄每晨灌胃加味青麟丸至24月龄；14～24JL组即先普食喂养至14月龄，然后开始每晨灌喂加味青麟丸至24月龄。测定血清IL-2水平、胸腺指数、脾脏指数。结果：14C组和14JL组大鼠脾脏指数、胸腺指数、血清IL-2水平均低于4C组（P〈0．05），14C组大鼠与14JL组比较无明显差异（P〉0．05）。24C组、24JL组和14～24JL组大鼠胸腺指数、脾脏指数、血清IL-2水平均明显低于4C组（P〈0．01）。24JL组明显高于24C组（P〈0．01），14～24JL组亦明显高于24C组（P〈0．01），24JL组与14～24JL组比较无明显差异（P〉0．05）。结论：加味青麟丸具有提高免疫力从而达到抗衰老的作用。

红树莓根茎煎剂对S180模型小鼠细胞因子水平和胸腺、脾指数的影响

目的:观测红树莓根茎煎剂(RRD)对S180模型小鼠血清细胞因子白细胞介素-2(IL-2)、白细胞介素-10(IL-10)水平和胸腺、脾脏指数的影响,探究肿瘤被RRD抑制的机理。方法:选50只昆明健康小鼠,雌雄各半,按随机原则分5组,正常对照组、模型对照组、环磷酰胺组(CTX组)、红树莓组(RRD组)及红树莓和环磷酰胺联合用药组(RRD+CTX组),每组10只。除正常对照组外,其余40只小鼠在右腋窝处注射0.2 mL S180瘤株混悬液做造模实验。第二天给小鼠灌胃,正常对照组和模型对照组:0.02 mL·g^(-1)·d^(-1)生理盐水灌胃;CTX组:0.4 mL·20 g^(-1)·d^(-1)生理盐水灌胃,20 mg·kg^(-1)·d^(-1) CTX腹腔注射;RRD组:0.4 mL·20 g^(-1)·d^(-1) RRD灌胃;RRD+CTX组:0.4 mL·20 g^(-1)·d^(-1)RRD灌胃,20 mg·kg^(-1)·d^(-1) CTX腹腔注射,连续10 d,1次/d。用酶联免疫吸附法测量血清IL-2、IL-10水平;测定胸腺、脾脏指数。结果:红树莓根茎水煎剂能使S180模型小鼠血清IL-2水平升高(P<0.05), IL-10水平下降(P<0.05);还能提升S180模型小鼠胸腺指数(P<0.05)、降低脾脏指数(P<0.05)。结论:红树莓根茎水煎剂的抑瘤作用很可能和调节机体的免疫抑制状态和提高荷瘤机体的免疫器官功能有关。

克癌7851对荷瘤小鼠造血与免疫功能的影响

本文观察了克癌7851对荷瘤小鼠骨髓造血和免疫功能的影响。结果表明,对照组每根股骨造血祖细胞数量为16856.7±3237.8,给药组为32355.7±4568.9。给药动物的胸腺及脾指数高于对照动物,对淋巴细胞转化及 IL-2产生也有不同程度的促进作用。

驱虫斑鸠菊提取物对小鼠免疫系统的影响

目的:探讨驱虫斑鸠菊提取物对小鼠免疫系统的影响。方法:驱虫斑鸠菊提取物高、中、低剂量组灌胃小鼠10 d,眼眶采血分离血清,用酶联免疫吸附法测定血清中IgA、IgG、IgM、C3、C4及IL-2的水平;解剖小鼠,测量小鼠胸腺和脾脏指数。以醋酸可的松作为阳性对照组,以生理盐水和溶媒作为空白照组和阴性对照。结果:1驱虫斑鸠菊提取物对小鼠免疫球蛋白IgA、IgG影响不大,对IgM、C3、C4有显著的降低作用;2驱虫斑鸠菊提取物对IL-2分泌有明显的抑制作用(P<0.01);3驱虫斑鸠菊提取物对正常小鼠胸腺指数、脾指数有显著降低作用(P<0.01)。结论:驱虫斑鸠菊提取物对小鼠免疫系统具有明显抑制作用。

衰老进程中机体免疫器官和免疫细胞因子变化及健脾补肾中药的干预研究

目的观察衰老进程中白细胞介素-2(IL-2)、白细胞介素-6(IL-6)水平和胸腺、脾脏指数的变化及健脾补肾中药干预的效应。方法将大鼠随机分为空白对照组,模型组,健脾补肾中药大、中、小治疗组,阳性对照组,每组8只。除空白对照组外,其余大鼠以D-半乳糖0.125 g/(kg.d)连续40 d皮下注射建立亚急性衰老大鼠模型,造模成功后健脾补肾中药组采用不同剂量健脾补肾中药干预,阳性对照组予桂附地黄丸干预。40 d后测定各组大鼠血清IL-2、IL-6水平和胸腺、脾脏指数。结果模型组大鼠血清IL-2和胸腺、脾脏指数显著降低(P<0.05),血清IL-6显著升高(P<0.05);健脾补肾中药组大鼠血清IL-2和胸腺、脾脏指数增高(P<0.05),血清IL-6显著降低(P<0.05)。结论健脾补肾中药具有调节衰老机体细胞因子失衡、抑制免疫器官退化和萎缩的作用。

金黄色葡萄球菌肠毒素A(SEA)对小鼠体内肉瘤抑制效果

以实验室建立的S180小鼠肿瘤模型为研究对象,采用腹腔注射给药,观察葡萄球菌肠毒素A(SEA)在体内抑制肿瘤的效果。实验表明,SEA抑肿瘤率为40.18%,显示对肿瘤有一定的抑制作用;能显著刺激脾脏细胞增殖,使脾指数升高至11.3mg/g;使血清和脾组织中IL-2水平分别升至69.77 pg/mL和682.43 pg/mL;且能诱导肿瘤组织中产生大量的CD4^+T细胞和CD8^+T细胞。结果显示,SEA在机体内对免疫功能有正向调节作用,从而在一定程度上抑制了肿瘤细胞的生长。

《金匮要略》“当先实脾”对肝损伤大鼠肝细胞凋亡的干预作用

目的寻求“见肝之病,知肝传脾,当先实脾”的生物学依据;探讨其对肝郁叠加慢性肝损伤大鼠模型肝细胞凋亡的影响。方法将48只大鼠采用随机数字表法分为6组,分别为正常组、模型组、双环醇组、柴芍六君子汤甲组、柴芍六君子汤乙组、柴芍六君子汤丙组,每组8只。模型组及各给药组采用束缚法叠加四氯化碳皮下注射法造模(5 mL/kg,体重,2次/7天),同时灌胃给药,连续42天。于光镜下观察肝组织病理切片;采用全自动生化分析仪检测血清肝功能生化指标;采用比色法检测5h-尿木糖排泄率;采用TUNEL法检测肝细胞凋亡指数(apoptotic index,AI);采用RT-PCR法及Western blot法分别检测肝组织B细胞淋巴瘤-2相关X蛋白(Bcl-2-associated X protein,Bax)、B细胞淋巴瘤-2(B-cell lymphoma-2,Bcl-2)基因及蛋白的表达。结果模型大鼠血清丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)显著升高,白蛋白(albumin,ALB)显著降低,肝组织存在较严重病理损伤;模型大鼠AI、Bax及Bax/Bcl-2均显著高于正常组,Bcl-2及5h-尿木糖排泄率对比正常组显著降低,“知肝传脾”有其生物学依据且与肝细胞凋亡相关。各干预方式均可不同程度减轻模型大鼠上述指标变化。“当先实脾”之柴芍六君子汤丙组对该模型各指标的调节作用优于其它干预方式。结论“见肝之病,知肝传脾,当先实脾”有其生物学依据,肝细胞凋亡为“见肝之病,知肝传脾,当先实脾”的生物学机制之一。

携带白细胞介素-2/肝细胞生长因子4-kringle拮抗剂双基因的减毒沙门菌对小鼠免疫功能的影响

目的研究携带白细胞介素-2(interleukin-2,IL⁃2)/肝细胞生长因子4-kringle拮抗剂(4-kringle antagonist of hepatocyte growth factor,NK4)双基因的减毒沙门菌(Ty21a-pIRES-IL-2-NK4,命名为TPIN)对小鼠体液及细胞免疫功能的影响。方法将18只BALB/c小鼠(雌雄各半)随机分为对照(胃管饲服1.5 mL 10%NaHCO3)、Ty21a(胃管饲服0.1 mL Ty21a)、TPIN(胃管饲服0.1 mL TPIN)组,6只/组。初次免疫7 d后加强免疫,共2次,给药第21天,经眼球采血分离血清,ELISA法检测血清IgG抗体水平;无菌分离小鼠胸腺和脾脏,体外分别用Ty21a、TPIN刺激培养的小鼠脾细胞,72 h后,CCK-8法检测小鼠脾细胞增殖能力,ELISA法检测小鼠脾细胞细胞因子表达水平;称重小鼠脾脏和胸腺,计算脾和胸腺指数,并进行脾和胸腺组织HE染色。结果与对照和Ty21a组相比,TPIN组小鼠血清IgG水平(F=111.74,P<0.01)以及脾细胞上清IFNγ、IL-4和IL-10含量均明显升高(F分别为38.21、11.37、26.92,P均<0.05),脾指数和胸腺指数明显升高(F分别为10.419、5.859,P均<0.05)。Ty21a、TPIN组小鼠胸腺组织皮质增宽,淋巴细胞增多,有轻度炎性反应;脾脏组织白髓增多,淋巴细胞增多伴随中性粒细胞浸润。结论TPIN具有良好的免疫保护作用,能明显刺激机体产生体液免疫和细胞免疫,从而对肿瘤可能具有较好的治疗作用。