Preparation, optical properties and cell staining of water soluble amine-terminated PAMAM G2.0-Au nanocomposites

The solution chemical and optical characteristics of formation of amine-terminated polyamidoamine dendrimer G2.0（NH2-PAMAM G2.0）-Au nanocomposites in the aqueous solution of NH2-PAMAM G2.0 at various mole ratios of Au（Ⅲ） to NH2-PAMAM G2.0 were studied by both UV-visible spectrometry and fluorospectrometry. The NH2-PAMAM G2.0-Au nanocomposites, with a type of structure in which one Au nanoparticle is surrounded by several NH2-PAMAM G2.0 dendrimers, emit strong bluish violet fluorescence, and are uniform, water soluble and biocompatible as well as very stable in frozen conditions. The size of gold nanoparticles in the nanocomposites is about 2.5 nm and decreases with the increase of NH2-PAMAM G2.0 concentration. The NH2-PAMAM G2.0 plays an important role in acting as host or micro-reactor for Au（Ⅲ） before Au（Ⅲ） reduction and acting as dispersant and stabilizer for gold nanoparticles after Au（Ⅲ） reduction. Preliminary experiments of cells staining to human embryonic lung fibroblast cell lines show that the NH2-PAMAM G2.0-Au nanocomposites can be used as optical imaging markers for bioanalyses and medical diagnoses.

Comparison of Hematoxylin-eosin Staining and Methyl Violet Staining for Displaying Ghost Cells

Purpose:.To compare the merits and limitations of hematoxylin-eosin(HE) and methyl violet staining for displaying ghost cells from vitreous or aqueous humor.Methods:.A specimen containing ghost cells was adjusted to five different concentrations:(12×104,.10×104,.8×104, 6×104and 4×104cells / ml) and subjected to smearing and methyl violet and HE staining..The staining results were observed by light microscopy.Results: The ghost cells were readily observed at a cell density of > 8×104cells / ml with methyl violet staining,.but only a few cells were occasionally seen at lower cell densities..In contrast,.ghost cells were seen at all cell densities with HE staining.Conclusion: Methyl violet staining is more rapid and simpler for the identification of ghost cells, but its staining color more readily fades, the slides cannot be stored, and it is only effective at a cell density of > 8 ×104cells / ml. In contrast,.HE staining is more time-consuming but it can display cell morphology and distinguish cell components more explicitly and slides can be permanently stored. HE staining has advantages over methyl violet staining in detecting the ghost cells when the concentration is < 8×104cells / ml.

CellDetect~染色技术在宫颈脱落细胞诊断中的价值

目的探讨CellDetect染色技术对阴道脱落细胞诊断及鉴别诊断作用。方法采用CellDetect染色对正常细胞和肿瘤细胞中颜色变化和细胞形态的相关性进行分析;并在子宫颈鳞状细胞癌(squmous cells carcinoma,SCC)和高级别上皮内瘤变(cervical intraepithelium neoplasia,CIN)的活检组织切片中比较CellDetect染色和HE染色的效果。结果 (1)手工涂片和TCT涂片的CellDetect染色均能较好的显示细胞的形态学特征,同时显示绝大多数正常鳞状上皮呈蓝色/绿色,子宫颈管上皮细胞呈鲜红色,不典型细胞与癌细胞呈红色。炎症细胞核呈红色,胞质大多数呈绿色/蓝色,红细胞胞质均呈绿色。(2)CIN和SCC的CellDetect染色诊断结果和HE染色诊断结果一致。(3)对子宫颈活检组织的切片和涂片分别行CellDetect染色,结果显示上皮原位细胞颜色反应相同,但CellDetect染色在判断癌与间质的关系或癌间质反应不如HE染色清晰。结论 Cell-Detect染色技术具有颜色和形态双重分析功能,适用于不同形式固定组织的细胞涂片染色,是子宫颈癌筛查和早期诊断的有效工具之一。

Detection of Mycoplasma Contamination in Animal Cell Cultures

[ Objective ] To develop a rapid efficient method for detecting mycoplasma contamination in cell cultures. [ Method] A pair of primers was designed according to two highly conserved nucleotide sequences of the 16S RNA from six kinds of mycoplasma that commonly contaminated cells. Then the mycoplasma contamination of 25 cell samples was defected by PCR and DNA fluorescence staining. EResultl When these cell samples were detected by DNA fluorescence staining, the positive rate and probable positive rate were respectively 24% and 16%. And when they were detected by PCR, the positive rate was 36%. [ Condusion] The PCR method is more sensitive and specific than the DNA fluorescence staining, and combining these two methods is the optimal way to detect mycoplasma contamination in cell cultures.

探讨DetectCell~染色在良恶性细胞鉴别中的价值

目的:用DetectCell染色人结肠癌细胞(HT-29)和小鼠胚胎成纤维细胞(C3H)细胞,探索DetectCell染色在细胞良恶性鉴别中的价值。方法:DetectCell染色试剂主要由含有酸性红,碱性绿和具有特殊反应亲和力的无花果属植物提取液组成。通过检测各主要染色试剂pH值、稀释浓度波长的吸光度比值(OD540/OD630)以控制染色试剂的检测标准,然后进行DetectCell染色观察细胞形态与颜色特征。结果:DetectCell主要染液的pH值和540 nm/630 nm波长处OD值均符合检测标准;DetectCell染色HT-29细胞和C3H细胞能保持原有细胞的形态,同时分别使纤维母细胞C3H被染成绿色,使癌细胞HT-29被染成红色。结论:DetectCell染色使癌细胞呈红色,非癌细胞呈绿色,并不影响细胞形态特征,因此该染色提供了颜色和形态双重分析功能。

Neurotoxic role of interleukin-17 in neural stem cell differentiation after intracerebral hemorrhage

Interleukin 17(IL-17)and its main producer,T cell receptorγδcells,have neurotoxic effects in the pathogenesis of intracerebral hemorrhage(ICH),aggravating brain injuries.To investigate the correlation between IL-17 and ICH,we dynamically screened serum IL-17 concentrations using enzyme-linked immunosorbent assay and explored the clinical values of IL-17 in ICH patients.There was a significant negative correlation between serum IL-17 level and neurological recovery status in ICH patients(r=–0.498,P<0.01).To study the neurotoxic role of IL-17,C57 BL/6 mice were used to establish an ICH model by injecting autologous blood into the caudate nucleus.Subsequently,the mice were treated with mouse neural stem cells(NSCs)and/or IL-17 neutralizing antibody for 72 hours.Flow cytometry,brain water content detection,Nissl staining,and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling results indicated that NSC transplantation significantly reduced IL-17 expression in peri-hematoma tissue,but there was no difference in T cell receptorγδcells.Compared with the ICH group,there were fewer apoptotic bodies and more Nissl bodies in the ICH+NSC group and the ICH+NSC+IL-17 group.To investigate the potential effect of IL-17 on directional differentiation of NSCs,we cultured mouse NSCs(NE-4 C)alone or co-cultured them with T cell receptorγδcells,which were isolated from mouse peripheral blood mononuclear cells,for 7 days.The results of western blot assays revealed that IL-17 secreted by T cell receptorγδcells reduced the differentiation of NSCs into astrocytes and neurons,while IL-17 neutralization relieved the inhibition of directional differentiation into astrocytes rather than neurons.In conclusion,serum IL-17 levels were elevated in the early stage of ICH and were negatively correlated with outcome in ICH patients.Animal experiments and cytological investigations therefore demonstrated that IL-17 probably has neurotoxic roles in ICH because of its inhibitory effects on the directional differentiation of NSCs.The application of IL-17 neutralizing antibody may promote the directional differentiation of NSCs into astrocytes.This study was approved by the Clinical Research Ethics Committee of Anhui Medical University of China(For human study:Approval No.20170135)in December 2016.All animal handling and experimentation were reviewed and approved by the Institutional Animal Care and Use Committee of Anhui Medical University(approval No.20180248)in December 2017.

Experimental Study of Cell Migration and Functional Differentiation of Transplanted Neural Stem Cells Co-labeled with Superparamagnetic Iron Oxide and Brdu in an Ischemic Rat Model

Objective To explore the migration of transplanted neural stem cells co-labeled with superparamagnetic iron oxide （SPIO） and bromodeoxyuridine （Brdu） using the 4.7T MR system and to study the cell differentiation with immuno-histochemical method in ischemic rats. Methods Rat neural stem cells （NSCs） co-labelled with SPIO mediated by poly-L-lysine and bromodeoxyuridine （BrdU） were transplanted into the unaffected side of rat brain with middle cerebral artery occlusion （MCAO）. At weeks 1, 2, 3, 4, 5, and 6 after MCAO, migration of the labelled cells was monitored by MRI. At week 6 the rats were killed and their brain tissue was cut according to the migration site of transplanted ceils indicated by MRI and subjected to Prussian blue staining and immunohistochemical staining to observe the migration and differentiation of the transplanted NSCs. Results Three weeks after transplantation, the linear hypointensity area derived from the migration of labelled NSCs was observed by MRI in the corpus callosum adjacent to the injection site. Six weeks after the transplantation, the linear hypointensity area was moved toward the midline along the corpus callosum. MRI findings were confirmed by Prussian blue staining and immunohistochemical staining of the specimen at week 6 after the transplantation. Flourescence co-labelled immunohistochemical methods demonstrated that the transplanted NSCs could differentiate into astrocytes and neurons. Conclusion MRI can monitor the migration of SPIO-labelled NSCs after transplantation in a dynamical and non-invasive manner. NSCs transplanted into ischemic rats can differentiate into astrocytes and neurons during the process of migration.

亚砷酸钠对人正常肝细胞脂质代谢及因子调控的作用

背景:肝脏作为砷毒作用的主要靶器官,成为砷毒性作用机制相关研究的焦点。目的:探讨亚砷酸钠对人正常肝细胞脂质代谢、细胞增殖、细胞凋亡及相关调控因子表达的影响。方法:MIHA正常人肝细胞株暴露于0,10,20,30μmol/L亚砷酸钠48 h,光学显微镜观察细胞形态变化,CCK-8法检测细胞活性,单试剂COD-PAP法、单试剂GPO-PAP法及酶微板法检测细胞上清总胆固醇、三酰甘油及总胆汁酸水平,油红O染色法检测细胞内脂质含量,Edu-488渗入法检测细胞增殖,PI染色法及Annexin V-FITC/PI双标记法结合流式细胞术分别检测细胞周期和细胞凋亡,实时荧光定量PCR和Western blot检测肝细胞核因子4α、胆固醇7α-羟化酶及法尼醇X受体的m RNA及蛋白表达水平。结果与结论:(1)与对照组(0μmol/L亚砷酸钠)相比,随着亚砷酸钠浓度的增加:MIHA细胞活性逐渐降低;细胞上清中总胆固醇、三酰甘油水平逐渐增高,总胆汁酸水平逐渐降低;细胞内脂质含量逐渐增多;细胞停滞于S期及G_2/M期细胞比例逐渐增多;细胞凋亡率逐渐升高;肝细胞核因子4α mRNA表达水平未见明显变化,胆固醇7α-羟化酶与法尼醇X受体mRNA表达水平下降;肝细胞核因子4α、胆固醇7α-羟化酶、法尼醇X受体蛋白表达水平逐渐下降;(2)亚砷酸钠具有细胞毒性,显著降低MIHA细胞活性,诱导细胞脂肪变性,阻滞细胞增殖,诱发细胞凋亡等损伤;亚砷酸钠可下调肝细胞核因子4α蛋白表达以及胆固醇7α-羟化酶、法尼醇X受体的转录和蛋白表达,进一步引起肝细胞的脂质代谢紊乱。

A comparing study of quantitative staining techniques for retinal neovascularization in a mouse model of oxygen-induced retinopathy

AIM: To explore an efficient, practical and objective quantitative method to evaluate the retinal neovascularization in mouse model of oxygen induced retinopathy (OIR). METHODS: Thirty C57BL/6J mice were explored in OIR model procedure. Eyes were removed for different staining methods including: (1) HE staining; (2) immunohistochemistry with Griffonia Simplicifolia Lectin (GSL); (3) Immunofluorescence with FITC labeled CD31 antibody; (4) Two-step immunofluorescence with purified-CD31 antibody; (5) FITC-Dextran perfusion combined with two-step purified-CD31immunofluorescence. Images of the retinal vasculature were analyzed by imaging software. ' RESULTS: GSL immunohistochemistry could clearly demonstrate the deep and superficial capillary beds. FITC labeled CD31 Immunofluorescence was blurring with high fluorescence background which was hard to distinguish retinal neovascularization in some area. Excellent detail of neovascularization and preexistent retinal vessels was provided in two-step Purified-CD31 immunofluorescence group. CONCLUSION: GSL immunohistochemistry can clearly demonstrate neovascularization tufts in deep and superficial capillary beds. Immunofluorescence of specific antigen CD31 on vascular endothelium can selectively label the neovascularization of mouse retina. When combined with computer analysis software, it is an effective and objective quantitative method to evaluate the retinal neovascularization in OIR mouse model.

DNA DIFFERENTIAL STAINING AND ITS CLINICAL APPLICATION

DNA differential stain is a simple method distinguishing cells of proliferative from quiescent stage. Double stranded DNA in quiescent cells is easily denatured by weak acid into single strand. As double stranded nucleic acid combined with methyl green and single stranded nucleic acid with pyronin, we make use of methyl green pyronin staining method to the cells treated with weak acid to distinguish proliferating from quiescent cells. This paper reports the observation of leukemia cells in the bone marrow smears of 100 cases of untreated acute leukemia by DNA differential staining method. The percentage of Go cells was lowest in ALL and highest in APL.

CDH1, a Novel Surface Marker of Spermatogonial Stem Cells in Sheep Testis

Spermatogonial stem cells（SSCs） are unique stem cells in adult body that can transmit genetic information to the next generation. They have self-renewal potential and can continuously support spermatogenesis throughout life of a male animal. However, the SSC population is extremely small, isolation and purification of the SSCs is challenging, especially for livestock animals. It has been confirmed that CDH1（cadherin-1, also known as E-cadherin） can be expressed in undifferentiated SSCs of mouse and rats, but it has not been verified in sheep. Here, CDH1 was found as a novel surface marker for sheep SSCs. In this paper, sheep antiCDH1 polyclonal antibodies were prepared and its activity was checked. Using the obtained antibodies and immunohistochemistry analysis, we confirmed that CDH1 can be expressed by SSCs in sheep testis.

Preliminary Research on a Mesenchymal Stem Cell-model for Screening Traditional Chinese Medicines

The authors focused their attention on the establishment of a mesenchymal stem cell（MSC） model for screening traditional Chinese medicines（TCMs） so as to investigate the effects of Shuanglong Formula（SLF） components（Ginsenosides and salvianolic acids） and ingredients（ginsenoside Rb1 and salvianolic acid B） on cardiomyocyte differentiation from MSCs.The SLF components were analyzed and quantified by HPLC-TOF-MS.Cardiomyocyte differentiation was induced by culturing MSCs in the induction medium supplemented with SLF ingredients,SLF components,5-azacytidine（5-aza）,5-aza＋SLF ingredients and 5-aza＋SLF components,respectively,for up to 30 d,and evulated by the expression of Cardiac-specific myosin heavy chain（MHC） and troponin I（TnI） via immunofluoresent staining.Slow growth rate and changed morphology were observed during cardiomyocyte differentiation.After 20 d of induction,differentiating MSCs were positive for MHC and TnI staining.The effects of SLF components were better than those of SLF ingredients.Taken together,SLF can induce the differentiation of MSCs into cardiomyogenic cells in vitro,and MSCs can be used as a powerful tool for screening TCMs.

Early therapeutic effect of platelet-rich fibrin combined with allogeneic bone marrow-derived stem cells on rats’ critical-sized mandibular defects

BACKGROUND Critically sized bone defects represent a significant challenge to orthopaedic surgeons worldwide.These defects generally result from severe trauma or resection of a whole large tumour.Autologous bone grafts are the current gold standard for the reconstruction of such defects.However,due to increased patient morbidity and the need for a second operative site,other lines of treatment should be introduced.To find alternative unconventional therapies to manage such defects,bone tissue engineering using a combination of suitable bioactive factors,cells,and biocompatible scaffolds offers a promising new approach for bone regeneration.AIM To evaluate the healing capacity of platelet-rich fibrin(PRF)membranes seeded with allogeneic mesenchymal bone marrow-derived stem cells(BMSCs)on critically sized mandibular defects in a rat model.METHODS Sixty-three Sprague Dawley rats were subjected to bilateral bone defects of critical size in the mandibles created by a 5-mm diameter trephine bur.Rats were allocated to three equal groups of 21 rats each.Group I bone defects were irrigated with normal saline and designed as negative controls.Defects of group II were grafted with PRF membranes and served as positive controls,while defects of group III were grafted with PRF membranes seeded with allogeneic BMSCs.Seven rats from each group were killed at 1,2 and 4 wk.The mandibles were dissected and prepared for routine haematoxylin and eosin(HE)staining,Masson's trichrome staining and CD68 immunohistochemical staining.RESULTS Four weeks postoperatively,the percentage area of newly formed bone was significantly higher in group III(0.88±0.02)than in groups I(0.02±0.00)and II(0.60±0.02).The amount of granulation tissue formation was lower in group III(0.12±0.02)than in groups I(0.20±0.02)and II(0.40±0.02).The number of inflammatory cells was lower in group III(0.29±0.03)than in groups I(4.82±0.08)and II(3.09±0.07).CONCLUSION Bone regenerative quality of critically sized mandibular bone defects in rats was better promoted by PRF membranes seeded with BMSCs than with PRF membranes alone.

肺癌病理应用支气管冲洗细胞块免疫组化链霉菌亲生物素蛋白-过氧化物酶连接法染色的诊断效果及检出率评价

目的探讨肺癌病理应用支气管冲洗细胞块免疫组化链霉菌亲生物素蛋白-过氧化物酶连接法(SP)染色的诊断效果及检出率。方法选取2017年2月至2021年12月广东省开平市中心医院收治的70例经支气管冲洗液检查阳性患者作为研究对象。采用液基薄层细胞学(TCT)技术制备细胞块,分别采用免疫组化SP法、苏木精伊红染色(HE)进行常规细胞学检查,比较两种检测方法诊断效能及不同肺癌类型免疫组化SP法检查后不同抗体阳性表达率比较。结果免疫组化SP法对恶性肿瘤诊断的灵敏度为96.15%、准确度为95.71%,均高于HE染色的69.23%、70.00%(P<0.05),特异度为94.44%,高于HE染色的72.22%,但差异无统计学意义。免疫组化SP法对肺癌病理类型总检出率高于HE染色,差异有统计学意义(P<0.05)。免疫组化SP法对肌上皮(p63)、细胞角蛋白5/6抗体(CK5/6)、细胞角蛋白7(CK7);甲状腺转录因子-1(TTF-1)、突触素(Syn)、人表皮生长因子受体-5(CD56),增殖细胞的核抗原(Ki67),天冬氨酸蛋白酶(Napsin-A)表达阳性率均高于HE染色,差异统计学意义(P<0.05)。不同类型肺癌患者p63、CK5/6、CK7、TTF-1、Syn、CD56、Ki67、Napsin-A阳性表达率比较差异有统计学意义(P<0.05)。结论支气管冲洗液细胞块免疫组化SP法染色对肺癌有较高的诊断价值,对不同类型肺癌有较高的检出率及鉴别率,具有重要的临床意义。

血小板内皮细胞黏附分子1和平滑肌肌动蛋白对瘢痕疙瘩综合治疗预后的影响

目的:探究瘢痕疙瘩血管内血小板内皮细胞黏附分子1(platelet endothelial cell adhesion molecule-1,PECAM-1)与平滑肌肌动蛋白(smooth muscle actin,SMA)的表达水平对瘢痕疙瘩综合治疗预后的影响。方法:回顾性分析2020年8月至2022年6月江苏大学附属医院皮肤科门诊收治的瘢痕疙瘩患者61例,共计69处瘢痕疙瘩,均接受手术切除与^(90)Sr同位素敷贴综合治疗,免疫组织化学染色检测手术切除瘢痕疙瘩标本血管内PECAM-1及SMA表达水平,电话及门诊随访6个月。结果:19处(27.5%)瘢痕疙瘩6个月内复发,11处(15.9%)在^(90)Sr同位素敷贴治疗后发生不良反应,复发组PECAM-1与SMA的高表达率均高于未复发组(χ^(2)=7.496,P=0.006;χ^(2)=5.197,P=0.023);治疗后不良反应发生率与PECAM-1及SMA的表达水平无明显关系(χ^(2)=0.172,P=0.935;χ^(2)=1.110,P=0.484)。结论:瘢痕疙瘩血管内PECAM-1及SMA的表达水平与综合治疗预后呈负相关,二者可能是判断瘢痕疙瘩预后的潜在指标。

Identification and characterization of cell cultures with various embryogenic/regenerative potential in cotton based on morphological,cytochemical,and cytogenetical assessment

Somatic embryogenesis(SE) plays a vital role in genetic transformation and massive propagation of important agronomical and economical crops.Here,we conducted a systematic assessment of the morphological,cytochemical,and cytogenetical characteristics of six culture strains with various embryogenic/regenerative potential during SE process in cotton.Results indicated that the six cell culture strains had stable ploidy levels,and did not reveal any relationship between the cytogenetic state and their morphogenetic potential.Moreover,the six culture strains were compared via double staining with Evans blue and Acetocarmine to efficiently distinguish embryogenic and non-embryogenic cells and determine the embryogenic nature of the calli.In addition,the kind of auxins added in medium affected not only growth property,color,size of cell clumps but also ploidy level and regeneration ability.By combining analysis of morphological,cytochemical,and cytogenetical characteristics of the cell cultures,we are able to obtain and maintain homogeneous cell population with high morphogenic and regeneration ability and establish efficient somatic embryogenesis and regeneration system from short-term cell cultures in upland cotton,which highlight the application of biotechnological approaches in crop breeding,and above all,to better understand totipotency of cells in higher plants.

宫颈液基细胞P16、Ki-67双染检测在1250例宫颈异常患者诊断中的诊断价值

目的:探讨与分析宫颈液基细胞P16、Ki-67双染检测在1250例宫颈异常患者诊断中的诊断价值。方法:选取2020年1月至2023年8月在河南省周口市中心医院进行宫颈疾病筛查的宫颈异常人群1250例作为研究对象,所有患者都给予宫颈液基细胞P16、Ki-67单染与双染检测,同时给予病理检查,根据病理学检测结果分为宫颈癌组(150例)和非宫颈癌组(1100例)。以病理检查作为诊断的金标准,判断以上检测方法对宫颈癌的诊断价值。结果:在1250例患者中,病理检查为宫颈上皮内瘤变(CIN)1级600例、CIN 2级320例、CIN 3级180例及宫颈癌150例,其中宫颈癌占比12.00%。宫颈癌组宫颈液基细胞P16、Ki-67单染阳性与P16、Ki-67双染阳性分别为98.00%、98.67%、97.33%,非宫颈癌组分别为40.55%、49.27%、21.00%,两组对比,差异有统计学意义(P<0.05)。在1250例患者中,宫颈液基细胞P16、Ki-67单染阳性与P16、Ki-67双染阳性对宫颈癌的诊断敏感性分别为98.00%(147/150)、98.67%(148/150)、97.33%(146/150),诊断特异性分别为59.45%(654/1100)、50.73%(558/1100)、79.00%(869/1100)。结论:宫颈异常患者中宫颈癌的发病率比较高,其宫颈液基细胞P16、Ki-67双染多表现为阳性状况,宫颈液基细胞P16、Ki-67双染检测在宫颈癌患者诊断中的应用可在保持敏感性的基础上,提高诊断特异性。

石蜡组织切片制作原理引入口腔组织病理学实验课的探讨

目的探讨石蜡组织切片制作原理引入本科口腔组织病理学实验课程的应用效果。方法2020年9—12月以自愿参加学习的郑州大学2017级口腔专业59名本科生为研究对象,实验课开课前讲授石蜡组织切片制作原理和HE染色理论知识,通过调查问卷的形式评价课程效果。结果调查问卷结果显示,57名学生(96.61%)认为该课程有助于实验课中组织切片学习且增加对实验课的兴趣。在口腔组织病理实验课中,39名学生(66.1%)观察到非学习组织结构或异常结构(情况),其中36名学生(92.31%)观察到的异常结构在课程中有讲述。结论石蜡组织切片制作原理引入本科口腔组织病理学实验课程,增强了学生对实验课的兴趣,有助于实验课中读片学习,有效提升了实验课的教学质量。

Induced Differentiation of Epithelioid Carcinoma Cell Lines: Evidence for Tumor Cell Quantal Mitosis

The effects of growth factors and calcium concentrations present in different culture media on induction of terminal differentiation were investigated for four different epidermoid carcinoma cell lines, Hela, KB, A431, and SCC-25, and their responses determined relative to those elicited by normal human keratinocytes subjected to these culture conditions. Differentiation status was determined cyto-chemically by a validated keratin protein staining method, and by autoradiographic analyses. Growth and differentiation promoting factors that influenced the direction of integrated control of growth and differentiation in normal human keratinocytes were found to be effective for some cell lines but not others. The factors examined were 1) high density arrest in serum-free and serum-containing media, 2) media shifts from high density culture in serum-containing media to low density growth factor-depleted or supplemented serum-free medium, and 3) the concentration of calcium in the media. The extent and degree of differentiation achieved varied among different cell lines depend on the presence or absence of serum, EGF and insulin protein growth factors. Certain growth media appear to sponsor keratin protein, cyto-chemically-detected differentiation, and evidence of quantal mitotic division in low density HeLa cell and SCC25 cell cultures. Epidermoid carcinoma cell lines retain limited capacity to commit to early stages of cell differentiation.

基于人工智能深度学习算法辅助诊断早期ESCC的研究

目的探讨基于人工智能(artificial intelligence,AI)深度学习算法的内镜识别系统在胃镜诊疗过程中对早期ESCC检出率的研究。方法选取中国人民解放军联勤保障部队第九八八医院、中国人民解放军联勤保障部队第九八四医院及中国人民解放军联勤保障部队第九八〇医院三个消化内镜中心2018年6月至2020年6月早期ESCC、ESCC、食管隆起性病变以及食管憩室的白光图像、碘染色图像。通过训练和验证不同的目标检测模型和实例分割模型,最终选取表现最优的目标检测模型Yolov 5和实例分割模型Yolact++共同构建AI“嵌合模型”,评估该模型诊断早期ESCC的性能。结果AI“嵌合模型”对早期ESCC诊断的敏感度为95.60%,特异度为91.60%,准确率为90.70%,均优于单模型。结论本研究构建的AI“嵌合模型”可显著提高早期ESCC的检出率。