目的:观察急性Stanford B型主动脉夹层(Aortic Dissection, AD)患者血浆代谢物丙酮酸水平变化,探讨其预测诊断价值。方法:选取2023年1月至2024年1在新疆医科大学第一附属医院诊治的急性Stanford B型AD患者及健康对照组,进行非靶向代谢...目的:观察急性Stanford B型主动脉夹层(Aortic Dissection, AD)患者血浆代谢物丙酮酸水平变化,探讨其预测诊断价值。方法:选取2023年1月至2024年1在新疆医科大学第一附属医院诊治的急性Stanford B型AD患者及健康对照组,进行非靶向代谢物组学检测及扩大人群ELISA实验验证。结果:筛选出胍基丁胺、N-乙酰苯丙氨酸、D-甘油酸等代谢物在AD组中含量明显高于HC组,分布差异有统计学意义(P Objective: Observing the changes in plasma metabolite pyruvate levels in patients with acute Stanford B aortic dissection (AD) and exploring its predictive diagnostic value. Method: We identified 28 patients with acute Stanford B AD treated at the First Affiliated Hospital of Xinjiang Medical University from January 2022 to July 2023, and matched them with 28 healthy control subjects. Conduct non targeted metabolomics testing and expand population ELISA experiments for validation. Results: The metabolites of Agmatine, N-Acetyl-L-phenylalanine, and D-glycerate Acid were significantly higher in the AD group than in the HC group, and the distribution difference was statistically significant (P < 0.001).The levels of metabolites such as N-2-Phospho-D-glyceric acid, L-Histidine, L-Citrulline, 1-Stearoyl-2-arachidonoyl-SN-glycerol, L-Lactic Acid, Pyruvic Acid, Sn-Glycero-3-Phosphocholine, L-Ornithine, L-Proline and Nicotinic Acid in the AD group were lower than those in the HC group, and the distribution difference was statistically significant (P < 0.001). Among them, pyruvate had the strongest correlation with aortic dissection (r = −0.835). Expanded population (AD = 82, HC = 82) was validated using the Enzyme Linked Immunosorbent Assay (ELISE) kit, and it was found that the content of pyruvate in the AD vs HC group was lower than that in the HC group, with statistical significance (AD: 1.527 ± 0.215;HC: 2.116 ± 0.249, AD vs HC, P < 0.001). Further correlation analysis between pyruvate and blood biochemical indicators in AD patients revealed that pyruvate was positively correlated with uric acid, triglycerides, total cholesterol, high-density, low-density, total bilirubin, and CK-MB, with Pearson correlation coefficients of 0.025, 0.015, 0.210, 0.264, 0.293, and 0.170, respectively. However, it was negatively correlated with urea, creatinine, blood glucose, total bilirubin, straight bile, unconjugated bilirubin bile, CK, D-dimer, C-reactive protein, interleukin-6, and procalcitonin, with Pearson correlation coefficients of −0.05, −0.215, −0.037, −0.265, −0.084. −0.098, −0.168, −0.392, −0.626, −0.457, −0.647. The final prediction of pyruvate distinguishes Stanford B AD and HC, with an AUC value of 0.959, 95% CI: 0.927~0.990, P < 0.001, Youden index of 0.878, sensitivity of 0.927, and specificity of 0.951. Conclusions: The levels of pyruvate in acute Stanford B AD are low, indicating good predictive diagnostic efficacy for acute Stanford B AD. It can serve as a potential new biomarker and provide a theoretical basis for exploring fast and sensitive predictive diagnostic methods in clinical practice.展开更多
文摘目的:观察急性Stanford B型主动脉夹层(Aortic Dissection, AD)患者血浆代谢物丙酮酸水平变化,探讨其预测诊断价值。方法:选取2023年1月至2024年1在新疆医科大学第一附属医院诊治的急性Stanford B型AD患者及健康对照组,进行非靶向代谢物组学检测及扩大人群ELISA实验验证。结果:筛选出胍基丁胺、N-乙酰苯丙氨酸、D-甘油酸等代谢物在AD组中含量明显高于HC组,分布差异有统计学意义(P Objective: Observing the changes in plasma metabolite pyruvate levels in patients with acute Stanford B aortic dissection (AD) and exploring its predictive diagnostic value. Method: We identified 28 patients with acute Stanford B AD treated at the First Affiliated Hospital of Xinjiang Medical University from January 2022 to July 2023, and matched them with 28 healthy control subjects. Conduct non targeted metabolomics testing and expand population ELISA experiments for validation. Results: The metabolites of Agmatine, N-Acetyl-L-phenylalanine, and D-glycerate Acid were significantly higher in the AD group than in the HC group, and the distribution difference was statistically significant (P < 0.001).The levels of metabolites such as N-2-Phospho-D-glyceric acid, L-Histidine, L-Citrulline, 1-Stearoyl-2-arachidonoyl-SN-glycerol, L-Lactic Acid, Pyruvic Acid, Sn-Glycero-3-Phosphocholine, L-Ornithine, L-Proline and Nicotinic Acid in the AD group were lower than those in the HC group, and the distribution difference was statistically significant (P < 0.001). Among them, pyruvate had the strongest correlation with aortic dissection (r = −0.835). Expanded population (AD = 82, HC = 82) was validated using the Enzyme Linked Immunosorbent Assay (ELISE) kit, and it was found that the content of pyruvate in the AD vs HC group was lower than that in the HC group, with statistical significance (AD: 1.527 ± 0.215;HC: 2.116 ± 0.249, AD vs HC, P < 0.001). Further correlation analysis between pyruvate and blood biochemical indicators in AD patients revealed that pyruvate was positively correlated with uric acid, triglycerides, total cholesterol, high-density, low-density, total bilirubin, and CK-MB, with Pearson correlation coefficients of 0.025, 0.015, 0.210, 0.264, 0.293, and 0.170, respectively. However, it was negatively correlated with urea, creatinine, blood glucose, total bilirubin, straight bile, unconjugated bilirubin bile, CK, D-dimer, C-reactive protein, interleukin-6, and procalcitonin, with Pearson correlation coefficients of −0.05, −0.215, −0.037, −0.265, −0.084. −0.098, −0.168, −0.392, −0.626, −0.457, −0.647. The final prediction of pyruvate distinguishes Stanford B AD and HC, with an AUC value of 0.959, 95% CI: 0.927~0.990, P < 0.001, Youden index of 0.878, sensitivity of 0.927, and specificity of 0.951. Conclusions: The levels of pyruvate in acute Stanford B AD are low, indicating good predictive diagnostic efficacy for acute Stanford B AD. It can serve as a potential new biomarker and provide a theoretical basis for exploring fast and sensitive predictive diagnostic methods in clinical practice.
