AIM:To reveal the functions of micro RNAs(mi RNAs) with respect to hepatic stellate cells(HSCs) in response to portal hypertension.METHODS:Primary rat HSCs were exposed to static water pressure(10 mm Hg,1 h) and the p...AIM:To reveal the functions of micro RNAs(mi RNAs) with respect to hepatic stellate cells(HSCs) in response to portal hypertension.METHODS:Primary rat HSCs were exposed to static water pressure(10 mm Hg,1 h) and the pressureinduced mi RNA expression profile was detected by next-generation sequencing. Quantitative real-time polymerase chain reaction was used to verify the expression of mi RNAs. A potential target of Mi R-9a-5p was measured by a luciferase reporter assay and Western blot. CCK-8 assay and Transwell assay were used to detect the proliferation and migration of HSCs under pressure.RESULTS:According to the profile,the expression of mi R-9a-5p was further confirmed to be significantly increased after pressure overload in HSCs(3.70 ± 0.61 vs 0.97 ± 0.15,P = 0.0226),which resulted in the proliferation,migration and activation of HSCs. In vivo,the up-regulation of mi R-9a-5p(2.09 ± 0.91 vs 4.27 ± 1.74,P = 0.0025) and the down-regulation of Sirt1(2.41 ± 0.51 vs 1.13 ± 0.11,P = 0.0006) were observed in rat fibrotic liver with portal hypertension. Sirt1 was a potential target gene of mi R-9a-5p. Through restoringthe expression of Sirt1 in mi R-9a-5p transfected HSCs on pressure overload,we found that overexpression of Sirt1 could partially abrogate the mi R-9a-5p mediated suppression of the proliferation,migration and activation of HSCs. CONCLUSION:Our results suggest that during liver fibrosis,portal hypertension may induce the proliferation,migration and activation of HSCs through the up-regulation of mi R-9a-5p,which targets Sirt1.展开更多
Saussurea plants have a long history of being used for disease prevention and control in Tibetan medicine, and the species have gradually changed along with the development of history. Saussurea is mainly effective in...Saussurea plants have a long history of being used for disease prevention and control in Tibetan medicine, and the species have gradually changed along with the development of history. Saussurea is mainly effective in wind-expelling, blood circulation, spasmolysis, pain relief, heat-clearing and detoxification. Characteristics, major chemical constituents and pharmacological studies of Saussurea stella Maxim. were summarized in this review based on the utilization of Saussurea medicinal plants in Tibetan areas, with the aim to provide adequate theoretical bases and references for exploring the further medicinal value and development and utilization of S. stella .展开更多
One of the threats to the modem men today is the quick change in seasons and unusual fluctuations in temperature Contemporary life is marked with various conditions that make life comparatively different and for that ...One of the threats to the modem men today is the quick change in seasons and unusual fluctuations in temperature Contemporary life is marked with various conditions that make life comparatively different and for that matter difficult for everyone. Amongst many worldly things, like materialism, struggle to survive in the highly competitive urban settings, people tend to consider the merits of cosmopolitanism more than anything else Curiously enough, Sir Philip Sidney＇s sonnets, though written in the 16th century and become milestones of the sonnet tradition in the English Renaissance which are applicable to today＇s global concern of environmentalism. When considered in the light of ecocriticism, Sidney＇s sonnets provide a suitable ground for the modem readers to reevaluate the current situation of the world we live in and the nature that we inherit from our ancestors. This paper aims to analyze some of Sidney＇s selected sonnets from Astrophil and Stella in the light of ecocritical approach to literary works.展开更多
基金Supported by National Natural Science Foundation of China,No.11272342/A0205
文摘AIM:To reveal the functions of micro RNAs(mi RNAs) with respect to hepatic stellate cells(HSCs) in response to portal hypertension.METHODS:Primary rat HSCs were exposed to static water pressure(10 mm Hg,1 h) and the pressureinduced mi RNA expression profile was detected by next-generation sequencing. Quantitative real-time polymerase chain reaction was used to verify the expression of mi RNAs. A potential target of Mi R-9a-5p was measured by a luciferase reporter assay and Western blot. CCK-8 assay and Transwell assay were used to detect the proliferation and migration of HSCs under pressure.RESULTS:According to the profile,the expression of mi R-9a-5p was further confirmed to be significantly increased after pressure overload in HSCs(3.70 ± 0.61 vs 0.97 ± 0.15,P = 0.0226),which resulted in the proliferation,migration and activation of HSCs. In vivo,the up-regulation of mi R-9a-5p(2.09 ± 0.91 vs 4.27 ± 1.74,P = 0.0025) and the down-regulation of Sirt1(2.41 ± 0.51 vs 1.13 ± 0.11,P = 0.0006) were observed in rat fibrotic liver with portal hypertension. Sirt1 was a potential target gene of mi R-9a-5p. Through restoringthe expression of Sirt1 in mi R-9a-5p transfected HSCs on pressure overload,we found that overexpression of Sirt1 could partially abrogate the mi R-9a-5p mediated suppression of the proliferation,migration and activation of HSCs. CONCLUSION:Our results suggest that during liver fibrosis,portal hypertension may induce the proliferation,migration and activation of HSCs through the up-regulation of mi R-9a-5p,which targets Sirt1.
基金Supported by the Fundamental Research Funds for the Central Universities:the Young Teachers Fund of Southwest Minzu University(2017NZYQN38)the Planning Project for Science and Technology Training of Sichuan Province(2016KZ0007)
文摘Saussurea plants have a long history of being used for disease prevention and control in Tibetan medicine, and the species have gradually changed along with the development of history. Saussurea is mainly effective in wind-expelling, blood circulation, spasmolysis, pain relief, heat-clearing and detoxification. Characteristics, major chemical constituents and pharmacological studies of Saussurea stella Maxim. were summarized in this review based on the utilization of Saussurea medicinal plants in Tibetan areas, with the aim to provide adequate theoretical bases and references for exploring the further medicinal value and development and utilization of S. stella .
文摘One of the threats to the modem men today is the quick change in seasons and unusual fluctuations in temperature Contemporary life is marked with various conditions that make life comparatively different and for that matter difficult for everyone. Amongst many worldly things, like materialism, struggle to survive in the highly competitive urban settings, people tend to consider the merits of cosmopolitanism more than anything else Curiously enough, Sir Philip Sidney＇s sonnets, though written in the 16th century and become milestones of the sonnet tradition in the English Renaissance which are applicable to today＇s global concern of environmentalism. When considered in the light of ecocriticism, Sidney＇s sonnets provide a suitable ground for the modem readers to reevaluate the current situation of the world we live in and the nature that we inherit from our ancestors. This paper aims to analyze some of Sidney＇s selected sonnets from Astrophil and Stella in the light of ecocritical approach to literary works.
