Defects-rich heterointerfaces integrated with adjustable crystalline phases and atom vacancies,as well as veiled dielectric-responsive character,are instrumental in electromagnetic dissipation.Conventional methods,how...Defects-rich heterointerfaces integrated with adjustable crystalline phases and atom vacancies,as well as veiled dielectric-responsive character,are instrumental in electromagnetic dissipation.Conventional methods,however,constrain their delicate constructions.Herein,an innovative alternative is proposed:carrageenan-assistant cations-regulated(CACR)strategy,which induces a series of sulfides nanoparticles rooted in situ on the surface of carbon matrix.This unique configuration originates from strategic vacancy formation energy of sulfides and strong sulfides-carbon support interaction,benefiting the delicate construction of defects-rich heterostructures in M_(x)S_(y)/carbon composites(M-CAs).Impressively,these generated sulfur vacancies are firstly found to strengthen electron accumulation/consumption ability at heterointerfaces and,simultaneously,induct local asymmetry of electronic structure to evoke large dipole moment,ultimately leading to polarization coupling,i.e.,defect-type interfacial polarization.Such“Janus effect”(Janus effect means versatility,as in the Greek two-headed Janus)of interfacial sulfur vacancies is intuitively confirmed by both theoretical and experimental investigations for the first time.Consequently,the sulfur vacancies-rich heterostructured Co/Ni-CAs displays broad absorption bandwidth of 6.76 GHz at only 1.8 mm,compared to sulfur vacancies-free CAs without any dielectric response.Harnessing defects-rich heterostructures,this one-pot CACR strategy may steer the design and development of advanced nanomaterials,boosting functionality across diverse application domains beyond electromagnetic response.展开更多
Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific capacities.However,the inferior intrinsic conductivity and large volume variation d...Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific capacities.However,the inferior intrinsic conductivity and large volume variation during sodiation-desodiation processes seriously affect its high-rate and long-cyde performance,unbeneficial for the application as fast-charging and long-cycling SIBs anode.Herein,the three-dimensional porous Cu_(1.81)S/nitrogen-doped carbon frameworks(Cu_(1.81)S/NC)are synthesized by the simple and facile sol-gel and annealing processes,which can accommodate the volumetric expansion of Cu_(1.81)S nanoparticles and accelerate the transmission of ions and electrons during Na^(+)insertion/extraction processes,exhibiting the excellent rate capability(250.6 mA·g^(-1)at 20.0 A·g^(-1))and outstanding cycling stability(70% capacity retention for 6000 cycles at 10.0 A·g^(-1))for SIBs.Moreover,the Na-ion full cells coupled with Na_(3)V_(2)(PO_(4))_(3)/C cathode also demonstrate the satisfactory reversible specific capacity of 330.5 mAh·g^(-1)at 5.0 A·g^(-1)and long-cycle performance with the 86.9% capacity retention at 2.0 A·g^(-1)after 750 cycles.This work proposes a promising way for the conversionbased metal sulfides for the applications as fast-charging sodium-ion battery anode.展开更多
The pathophysiology of Huntington's disease involves high levels of the neurotoxin quinolinic acid. Quinolinic acid accumulation results in oxidative stress, which leads to neurotoxicity. However, the molecular an...The pathophysiology of Huntington's disease involves high levels of the neurotoxin quinolinic acid. Quinolinic acid accumulation results in oxidative stress, which leads to neurotoxicity. However, the molecular and cellular mechanisms by which quinolinic acid contributes to Huntington's disease pathology remain unknown. In this study, we established in vitro and in vivo models of Huntington's disease by administering quinolinic acid to the PC12 neuronal cell line and the striatum of mice, respectively. We observed a decrease in the levels of hydrogen sulfide in both PC12 cells and mouse serum, which was accompanied by down-regulation of cystathionine β-synthase, an enzyme responsible for hydrogen sulfide production. However, treatment with NaHS(a hydrogen sulfide donor) increased hydrogen sulfide levels in the neurons and in mouse serum, as well as cystathionine β-synthase expression in the neurons and the mouse striatum, while also improving oxidative imbalance and mitochondrial dysfunction in PC12 cells and the mouse striatum. These beneficial effects correlated with upregulation of nuclear factor erythroid 2-related factor 2 expression. Finally, treatment with the nuclear factor erythroid 2-related factor 2inhibitor ML385 reversed the beneficial impact of exogenous hydrogen sulfide on quinolinic acid-induced oxidative stress. Taken together, our findings show that hydrogen sulfide reduces oxidative stress in Huntington's disease by activating nuclear factor erythroid 2-related factor 2,suggesting that hydrogen sulfide is a novel neuroprotective drug candidate for treating patients with Huntington's disease.展开更多
目的肝星状细胞(HSC)和硫化氢(H2S)信号分子作为肝细胞癌(HCC)微环境中重要的组分,参与调控HCC的发生发展等多种生物学进程。本研究通过HSC与肝癌细胞系共培养,探讨HSC通过分泌H2S参与调控肝癌细胞凋亡的作用及其机制。方法以HSC细胞系(...目的肝星状细胞(HSC)和硫化氢(H2S)信号分子作为肝细胞癌(HCC)微环境中重要的组分,参与调控HCC的发生发展等多种生物学进程。本研究通过HSC与肝癌细胞系共培养,探讨HSC通过分泌H2S参与调控肝癌细胞凋亡的作用及其机制。方法以HSC细胞系(LX-2)及肝癌细胞系(Hep G2、PLC/PRF/5)为研究对象。RT-q PCR和Western Blot(WB)法检测LX-2中H2S关键合成酶——胱硫醚γ-裂解酶(CSE)m RNA及表达水平;ELISA测定上清液LX-2产生的H2S浓度;二代测序、细胞免疫荧光、染色质免疫沉淀(Ch IP)及WB检测H2S(内源性和外源性)作用Hep G2和PLC/PRF/5细胞后,JNK/Jun B-TNFSF14信号通路基因、结合位点及相关蛋白。Transwell小室将LX-2分别与Hep G2和PLC/PRF/5共培养,CCK-8和流式细胞术检测肝癌细胞的细胞活力、凋亡,WB测定H2S-TNFSF14信号通路相关蛋白。所有细胞实验均重复3次。计量资料两组间比较采用成组t检验;多组间比较采用单因素方差分析或重复测量方差分析,进一步两两比较采用Dunnett-t检验。结果LX-2主要通过CSE合成H2S,LX-2培养上清液中H2S浓度随着时间延长逐渐增加[(22.89±0.08)pg/m L vs(28.29±0.15)pg/m L vs(36.19±1.90)pg/m L,F=79.63,P<0.05]。LX-2中CSE m RNA水平显著高于CBS m RNA和MPST m RNA(1.008±0.13 vs 0.320±0.014 vs 0.05±0.02,F=80.84,P<0.05)。当CSE被炔丙基甘氨酸(PPG)抑制之后,随着PPG浓度增加,H2S浓度下降(P<0.05)。LX-2分别与Hep G2、PLC/PRF/5共培养,随着培养时间延长,Hep G2(87.48%±0.82%vs70.48%±0.641%vs 52.89%±0.57%vs 45.20%±0.69%,F=1517.13,P<0.001)和PLC/PRF/5(92.41%±0.48%vs 74.10%±0.73%vs 53.70%±0.60%vs 44.00%±0.27%,F=2626.21,P<0.001)细胞活力降低;凋亡增加(Hep G2:12.88%±0.64%vs15.5%±0.16%vs 18.43%±0.37%vs 13.01%±0.58%,F=142.15,P<0.001;PLC/PRF/5:8.51±0.05 vs 12.80±0.33 vs 15.59±0.21 vs 10.72±0.30,F=676.40,P<0.001),第3天最显著。二代测序显示,内源性H2S(LX-2产生)和Na HS(外源性H2S供体)参与调控Hep G2中多种基因表达。Na HS和LX-2通过释放H2S,激活肝癌细胞中JNK/Jun B信号通路、上调凋亡基因TNFSF14表达,且p-Jun B与TNFSF14基因转录调控区域结合增加。结论在肝癌微环境中,HSC通过信号分子CSE/H2S,激活了肝癌细胞中JNK/Jun B信号通路,TNFSF14表达增加,从而促进了肝癌细胞凋亡。展开更多
基金financially supported by the National Natural Science Foundation of China(Grants nos.62201411,62371378,22205168,52302150 and 62304171)the China Postdoctoral Science Foundation(2022M722500)+1 种基金the Fundamental Research Funds for the Central Universities(Grants nos.ZYTS2308 and 20103237929)Startup Foundation of Xidian University(10251220001).
文摘Defects-rich heterointerfaces integrated with adjustable crystalline phases and atom vacancies,as well as veiled dielectric-responsive character,are instrumental in electromagnetic dissipation.Conventional methods,however,constrain their delicate constructions.Herein,an innovative alternative is proposed:carrageenan-assistant cations-regulated(CACR)strategy,which induces a series of sulfides nanoparticles rooted in situ on the surface of carbon matrix.This unique configuration originates from strategic vacancy formation energy of sulfides and strong sulfides-carbon support interaction,benefiting the delicate construction of defects-rich heterostructures in M_(x)S_(y)/carbon composites(M-CAs).Impressively,these generated sulfur vacancies are firstly found to strengthen electron accumulation/consumption ability at heterointerfaces and,simultaneously,induct local asymmetry of electronic structure to evoke large dipole moment,ultimately leading to polarization coupling,i.e.,defect-type interfacial polarization.Such“Janus effect”(Janus effect means versatility,as in the Greek two-headed Janus)of interfacial sulfur vacancies is intuitively confirmed by both theoretical and experimental investigations for the first time.Consequently,the sulfur vacancies-rich heterostructured Co/Ni-CAs displays broad absorption bandwidth of 6.76 GHz at only 1.8 mm,compared to sulfur vacancies-free CAs without any dielectric response.Harnessing defects-rich heterostructures,this one-pot CACR strategy may steer the design and development of advanced nanomaterials,boosting functionality across diverse application domains beyond electromagnetic response.
基金financially supported by the National Natural Science Foundation of China(Nos.U1904173 and 52272219)the Key Research Projects of Henan Provincial Department of Education(No.19A150043)+2 种基金the Natural Science Foundation of Henan Province(Nos.202300410330 and 222300420276)the Nanhu Scholars Program for Young Scholars of Xinyang Normal Universitythe Xinyang Normal University Analysis&Testing Center。
文摘Transition metal sulfides have great potential as anode mterials for sodium-ion batteries(SIBs)due to their high theoretical specific capacities.However,the inferior intrinsic conductivity and large volume variation during sodiation-desodiation processes seriously affect its high-rate and long-cyde performance,unbeneficial for the application as fast-charging and long-cycling SIBs anode.Herein,the three-dimensional porous Cu_(1.81)S/nitrogen-doped carbon frameworks(Cu_(1.81)S/NC)are synthesized by the simple and facile sol-gel and annealing processes,which can accommodate the volumetric expansion of Cu_(1.81)S nanoparticles and accelerate the transmission of ions and electrons during Na^(+)insertion/extraction processes,exhibiting the excellent rate capability(250.6 mA·g^(-1)at 20.0 A·g^(-1))and outstanding cycling stability(70% capacity retention for 6000 cycles at 10.0 A·g^(-1))for SIBs.Moreover,the Na-ion full cells coupled with Na_(3)V_(2)(PO_(4))_(3)/C cathode also demonstrate the satisfactory reversible specific capacity of 330.5 mAh·g^(-1)at 5.0 A·g^(-1)and long-cycle performance with the 86.9% capacity retention at 2.0 A·g^(-1)after 750 cycles.This work proposes a promising way for the conversionbased metal sulfides for the applications as fast-charging sodium-ion battery anode.
基金supported by the National Natural Science Foundation of China,Nos.82271327 (to ZW),82072535 (to ZW),81873768 (to ZW),and 82001253 (to TL)。
文摘The pathophysiology of Huntington's disease involves high levels of the neurotoxin quinolinic acid. Quinolinic acid accumulation results in oxidative stress, which leads to neurotoxicity. However, the molecular and cellular mechanisms by which quinolinic acid contributes to Huntington's disease pathology remain unknown. In this study, we established in vitro and in vivo models of Huntington's disease by administering quinolinic acid to the PC12 neuronal cell line and the striatum of mice, respectively. We observed a decrease in the levels of hydrogen sulfide in both PC12 cells and mouse serum, which was accompanied by down-regulation of cystathionine β-synthase, an enzyme responsible for hydrogen sulfide production. However, treatment with NaHS(a hydrogen sulfide donor) increased hydrogen sulfide levels in the neurons and in mouse serum, as well as cystathionine β-synthase expression in the neurons and the mouse striatum, while also improving oxidative imbalance and mitochondrial dysfunction in PC12 cells and the mouse striatum. These beneficial effects correlated with upregulation of nuclear factor erythroid 2-related factor 2 expression. Finally, treatment with the nuclear factor erythroid 2-related factor 2inhibitor ML385 reversed the beneficial impact of exogenous hydrogen sulfide on quinolinic acid-induced oxidative stress. Taken together, our findings show that hydrogen sulfide reduces oxidative stress in Huntington's disease by activating nuclear factor erythroid 2-related factor 2,suggesting that hydrogen sulfide is a novel neuroprotective drug candidate for treating patients with Huntington's disease.
文摘目的肝星状细胞(HSC)和硫化氢(H2S)信号分子作为肝细胞癌(HCC)微环境中重要的组分,参与调控HCC的发生发展等多种生物学进程。本研究通过HSC与肝癌细胞系共培养,探讨HSC通过分泌H2S参与调控肝癌细胞凋亡的作用及其机制。方法以HSC细胞系(LX-2)及肝癌细胞系(Hep G2、PLC/PRF/5)为研究对象。RT-q PCR和Western Blot(WB)法检测LX-2中H2S关键合成酶——胱硫醚γ-裂解酶(CSE)m RNA及表达水平;ELISA测定上清液LX-2产生的H2S浓度;二代测序、细胞免疫荧光、染色质免疫沉淀(Ch IP)及WB检测H2S(内源性和外源性)作用Hep G2和PLC/PRF/5细胞后,JNK/Jun B-TNFSF14信号通路基因、结合位点及相关蛋白。Transwell小室将LX-2分别与Hep G2和PLC/PRF/5共培养,CCK-8和流式细胞术检测肝癌细胞的细胞活力、凋亡,WB测定H2S-TNFSF14信号通路相关蛋白。所有细胞实验均重复3次。计量资料两组间比较采用成组t检验;多组间比较采用单因素方差分析或重复测量方差分析,进一步两两比较采用Dunnett-t检验。结果LX-2主要通过CSE合成H2S,LX-2培养上清液中H2S浓度随着时间延长逐渐增加[(22.89±0.08)pg/m L vs(28.29±0.15)pg/m L vs(36.19±1.90)pg/m L,F=79.63,P<0.05]。LX-2中CSE m RNA水平显著高于CBS m RNA和MPST m RNA(1.008±0.13 vs 0.320±0.014 vs 0.05±0.02,F=80.84,P<0.05)。当CSE被炔丙基甘氨酸(PPG)抑制之后,随着PPG浓度增加,H2S浓度下降(P<0.05)。LX-2分别与Hep G2、PLC/PRF/5共培养,随着培养时间延长,Hep G2(87.48%±0.82%vs70.48%±0.641%vs 52.89%±0.57%vs 45.20%±0.69%,F=1517.13,P<0.001)和PLC/PRF/5(92.41%±0.48%vs 74.10%±0.73%vs 53.70%±0.60%vs 44.00%±0.27%,F=2626.21,P<0.001)细胞活力降低;凋亡增加(Hep G2:12.88%±0.64%vs15.5%±0.16%vs 18.43%±0.37%vs 13.01%±0.58%,F=142.15,P<0.001;PLC/PRF/5:8.51±0.05 vs 12.80±0.33 vs 15.59±0.21 vs 10.72±0.30,F=676.40,P<0.001),第3天最显著。二代测序显示,内源性H2S(LX-2产生)和Na HS(外源性H2S供体)参与调控Hep G2中多种基因表达。Na HS和LX-2通过释放H2S,激活肝癌细胞中JNK/Jun B信号通路、上调凋亡基因TNFSF14表达,且p-Jun B与TNFSF14基因转录调控区域结合增加。结论在肝癌微环境中,HSC通过信号分子CSE/H2S,激活了肝癌细胞中JNK/Jun B信号通路,TNFSF14表达增加,从而促进了肝癌细胞凋亡。
