Correlation between Nrf2‑GPX4 signaling pathway and patients with coronary heart disease

Objective:To analyze the correlation between serum Nrf2 and GPX4 activity levels with coronary heart disease(CHD)and the severity of coronary artery disease,and to explore the role of ferroptosis mediated by its signaling pathway in the progression of CHD.Methods:A total of 540 patients suspected of CHD were selected for coronary angiography,of which 360 patients were diagnosed with CHD.The activity levels of Nrf2 and GPX4 in the serum of CHD patients were detected by ELISA,and the differences in the activities of Nrf2 and GPX4 in the presence or absence of CHD were statistically analyzed.Western blot detection of Nrf2 protein expression of peripheral blood mononuclear cells(PBMCS)in the control(CON)and CHD groups(90 cases each).The expression and significance of its signaling pathway in CHD patients were analyzed.Results:The activity levels of Nrf2 and GPX4 in the CHD group were lower than those in the CON group(P<0.05),and the expression levels of Nrf2 in PBMCs of the two groups were detected by Western blot.The protein expression level of Nrf2 in the CHD group(0.25±0.05)was down‑regulated compared with CON group(0.87±0.16)(P<0.05),indicating that Nrf2 protein expression level was low in CHD patients.Pearson correlation analysis showed that serum Nrf2 and GPX4 levels were negatively correlated with Gensini score(Nrf2:r=‑0.347,P<0.001;GPX4:r=-0.423,P=0.001).Nrf2 and GPX4 were negatively correlated with TG(Nrf2:r=-0.284,P<0.001;GPX4:r=-0.275,P=0.001),Nrf2 and GPX4 levels were negatively correlated with LDL(Nrf2:r=-0.418,P<0.001)0.05;(GPX4:r=-0.426,P<0.05),Nrf2 and GPX4 levels were positively correlated with HDL(Nrf2:r=0.318,P<0.05;GPX4:r=0.428,P<0.05),and Nrf2 was positively correlated with GPX4(r=0.456,P<0.01).Conclusion:The ferroptosis pathway mediated by the Nrf2‑GPX4 signaling pathway is closely related to the degree of coronary artery disease and the pathogenesis of CHD,and its mechanism may be related to the down‑regulation of the Nrf2‑GPX4 signaling pathway.

Significance of changes of serum ICAM-1 and LDH activity in different severity of vascular lesions of coronary heart disease

Objective:To analyze the relationship between serum ICAM-1,lactate dehydrogenase(LDH)and coronary heart disease(CHD)by clinical detection of serum ICAM-1 and LDH Objective to investigate the relationship between the severity of different types of coronary artery stenosis(CHD)and its role in the progression of CHD.Methods:180 patients with chest pain and coronary angiography were selected,including 145 patients with CHD diagnosed by coronary angiography and 35 patients without abnormal coronary angiography(control group).The 145 patients with CHD were further divided into mild partial stenosis group(a-chd,stenosis development≤49%),moderate partial stenosis group(b-chd,stenosis development 50%~74%)and severe partial stenosis group(c-chd,stenosis development≥75%).The levels of ICAM-1 and LDH in serum of patients were detected by ELISA,and the differences of ICAM-1 and LDH activities in different degrees of coronary artery disease and control group were statistically analyzed.Results:in the control group,HDL was higher than that in the c-chd group(P<0.05),neutrophils in the control group was lower than that in the c-chd group(P<0.05);ICAM-1,LDH and Gensini scores in the a-chd group,b-chd group and c-chd group were higher than those in the control group(P<0.05-P<0.01),and gradually increased with the aggravation of different coronary artery stenosis processes(P<0.05-P<0.01).The levels of serum ICAM-1 and LDH increased significantly with the increase of the number of CHD lesions(P<0.01).The levels of ICAM-1 and LDH were positively correlated with Gensini score(LDH r=0.409,P<0.01 and ICAM-1 r=0.463,P<0.01).The levels of ICAM-1 and neutrophils were positively correlated(r=0.208,P<0.01).The levels of LDH and ICAM-1 were negatively correlated with HDL(LDH r=-0.184,P<0.05 and ICAM-1)There was a negative correlation between LDH and total protein(r=-0.218,P<0.01),and a positive correlation between ICAM-1 and LDH(r=0.441,P<0.01).Conclusion:the levels of serum ICAM-1 and LDH gradually increase with the aggravation of different coronary artery stenosis,and there is a certain linear relationship between them.