Two new Steroidal alkaloids, named pengbeisine A and pengbeisine B, were isolated from the fresh bulbs of Fritillaria monatha Migo. along with a known alkaloid. The structures were characterized as 22,26-imino-17, 23-...Two new Steroidal alkaloids, named pengbeisine A and pengbeisine B, were isolated from the fresh bulbs of Fritillaria monatha Migo. along with a known alkaloid. The structures were characterized as 22,26-imino-17, 23-oxido-5α-jerv-6-oxo-2α,3β-diol and 22, 26-imino-17, 23-oxido-5α-jerv-6-oxo-2β,3α-diol on the basis of spectroscopic methods.展开更多
Two new steroidal alkaloids, veraussines A (1) and B (2) were isolated from the roots and rhizomes of Veratrum nigrum var. ussuriense. Their structures were determined as N-(ethoxycarbonyl)- 1 α,2β,3α, 15α-t...Two new steroidal alkaloids, veraussines A (1) and B (2) were isolated from the roots and rhizomes of Veratrum nigrum var. ussuriense. Their structures were determined as N-(ethoxycarbonyl)- 1 α,2β,3α, 15α-tetrahydroxy-5fl-jervanin- 12-en- 11 -one (1) and N-(methoxycarbonyl)-1α,2β,3α,15α-tetrahydroxy-5/3-jervanin-12-en-1 l-one (2) by spectroscopic analysis.展开更多
Steroidal alkaloids possess the basic steroidal skeleton with a nitrogen atom in rings or side chains incorporated as an integral part of the molecule.They have demonstrated a wide range of biological activities,and s...Steroidal alkaloids possess the basic steroidal skeleton with a nitrogen atom in rings or side chains incorporated as an integral part of the molecule.They have demonstrated a wide range of biological activities,and some of them have even been developed as therapeutic drugs,such as abiraterone acetate(Zytiga®),a blockbuster drug,which has been used for the treatment of prostate cancer.Structurally diverse natural steroidal alkaloids present a wide spectrum of biological activities,which are attractive for natural product chemistry and medicinal chemistry communities.This review comprehensively covers the structural classification,isolation and various biological activities of 697 natural steroidal alkaloids discovered from 1926 to October 2021,with 363 references being cited.展开更多
A new steroidal alkaloid, vaganine A (1) was isolated together with a known compound paohysandrine A (2) from Sarcooocca vagans. Their structures were determined by spectroscopic evidence.
From the bulbs of Fritillaria siechuanica S.C.Chen cultivated in Sichuan province,a new steroidal alkaloid,siechuansine,was isolated.Spectral methods demonstrated that it is a 13—epimer of wanpeinine A.
Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-a-induced human RA-FL...Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-a-induced human RA-FLS MH7 A in vitro, and against complete Freund's adjuvant(CFA)-induced arthritis rats in vivo. Methods: CCK-8 assay was utilized to evaluate the anti-proliferation activity in vitro. In in-vivo study, rats were randomly divided into control group, model group(CFA), steroidal alkaloids(STA) groups(5.0, 2.5 and 1.25 g/kg BW),and sarcovagine D(SD) groups(50, 100 and 200 mg/kg BW), 10 rats for each group. To evaluate the anti-inflammation effect, the histology, biochemical parameters and expression of inflammatory cytokines were detected. Results: Steroidal alkaloids and sarcovagine D showed strong anti-proliferative activity during MH7 A cell culture proliferation and downregulated NO levels, and inflammatory cytokines(IL-1β, IL-6 and PGE_2) in vitro. The rats treated with CFA induced ankle inflammation and lymphocyte infiltration, upregulated IL-1β, TNF-α,IL-6, PGE_2, MDA and NO levels, and downregulated IL-10 and SOD. On the contrary,steroidal alkaloids ameliorated all the changes induced by CFA in rats, suggesting the antiinflammatory effect of steroidal alkaloids and sarcovagine D. Conclusions: The results suggest that Sarcococca hookeriana var. digyna has anti-inflammatory effect. Steroidal alkaloids and sarcovagine D has the potential to cure RA.展开更多
Two new D/E trans-5(?)-cevanine alkaloids,Dongbernine and Dongbeirine,have been isolated from Fritillaria thunbergii Miq.var.chekiangensis together with six known compounds,peimine,peiminine,isoverticine,verticindiol,...Two new D/E trans-5(?)-cevanine alkaloids,Dongbernine and Dongbeirine,have been isolated from Fritillaria thunbergii Miq.var.chekiangensis together with six known compounds,peimine,peiminine,isoverticine,verticindiol,B—sitosterol daucoterol.展开更多
The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chr...The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chromatography using Avicel as the adsorbent. After that, 50% of methanol in water was used as the eluting solvent and continued to further purify by Avicel preparative layer chromatogram 2 times using 80% of methanol in water as developing solvent. MIC of pure compound was done by two fold dilution method. Muller Hinton agar was selected as the media. The new compound was found as combretin, a steroidal alkaloid, and showed antibacterial activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 better than Staphylococcus aureus ATCC 25923.展开更多
Objective To design and synthesize derivatives of wanpeinine A,the main steroidal alkaloid isolated from the plant Fritillaria shuchengensis,and further study on the structure-activity relationship of the steroidal al...Objective To design and synthesize derivatives of wanpeinine A,the main steroidal alkaloid isolated from the plant Fritillaria shuchengensis,and further study on the structure-activity relationship of the steroidal alkaloid.Methods Acylation and alkylation were used to synthesize the derivatives and their structures were identified via NMR and MS.Results The acylation of wanpeinine A(1) produced 3β,6α-diacetylwanpeinine A(2),3β,6α-dipropionyl-wanpeinine A(3),3β,6α-dichloracetylwanpeinine A(4),3β,6α-dibenzoylwanpeinine A(5),and 3β-methoxy-acylwanpeinine A(6).The alkylation of wanpeinine A formed 3β,6α-dimethoxymethylwanpeinine A(7).Conclusion All compounds are new except for 3β,6α-diacetylwanpeinine A.展开更多
Solanum lyratum is one of the temperate plants,broadly distributed in Korea,China,Japan,India,and South-East Asia and well-documented in those oriental ethnic medicine systems for curing cancers,jaundice,edema,gonorrh...Solanum lyratum is one of the temperate plants,broadly distributed in Korea,China,Japan,India,and South-East Asia and well-documented in those oriental ethnic medicine systems for curing cancers,jaundice,edema,gonorrhea,cholecystitis,phlogosis,rheumatoid arthritis,etc.This review systematically summarized the research progress on S.lyratum respecting the botany,traditional uses,phytochemistry,pharmacology,and toxicology to increase people’s in-depth understanding of this plant,by data retrieval in a series of online or off-line electronic databases as far as we can reach.Steroidal saponins and alkaloids,terpenoids,nitrogenous compounds,and flavonoid compounds are the main chemical constituents in S.lyratum.Among them,steroidal alkaloids and saponins are the major active ingredi-ents ever found in S.lyratum,exerting activities of anti-cancer,anti-inflammation,anti-microbial,anti-allergy,and anti-oxidation in vivo or in vitro.As a result,S.lyratum has been frequently prescribed for the abovementioned therapeutic purposes,and there are substantial traditional and modern shreds of evidence of its use.展开更多
Over the past 30 years,the genus Solanum has received considerable attention in chemical and biological studies.Solanum is the largest genus in the family Solanaceae,comprising of about 2000 species distributed in the...Over the past 30 years,the genus Solanum has received considerable attention in chemical and biological studies.Solanum is the largest genus in the family Solanaceae,comprising of about 2000 species distributed in the subtropical and tropical regions of Africa,Australia,and parts of Asia,e.g.,China,India and Japan.Many of them are economically signifcant species.Previous phytochemical investigations on Solanum species led to the identifcation of steroidal saponins,steroidal alkaloids,terpenes,favonoids,lignans,sterols,phenolic comopunds,coumarins,amongst other compounds.Many species belonging to this genus present huge range of pharmacological activities such as cytotoxicity to diferent tumors as breast cancer(4T1 and EMT),colorectal cancer(HCT116,HT29,and SW480),and prostate cancer(DU145)cell lines.The biological activities have been attributed to a number of steroidal saponins,steroidal alkaloids and phenols.This review features 65 phytochemically studied species of Solanum between 1990 and 2018,fetched from SciFinder,Pubmed,ScienceDirect,Wikipedia and Baidu,using“Solanum”and the species’names as search terms(“all felds”).展开更多
Objective:To determine the phytochemicals and screen the antimicrobial potential of Desmococcus olivaceus(D.olivaceus)and Chlorella vulgaris(C.vulgaris)against human bacterial pathogens.Methods:D.olivaceus and C.vulga...Objective:To determine the phytochemicals and screen the antimicrobial potential of Desmococcus olivaceus(D.olivaceus)and Chlorella vulgaris(C.vulgaris)against human bacterial pathogens.Methods:D.olivaceus and C.vulgaris were collected and confirmed through morphological structures.Phytochemicals were analyzed and confirmed by chemical methods.Thin layer chromatography was performed to evaluate the bioactive compounds present in the solvent extracts.Antimicrobial activity of the extract obtained from dried green microalgae was tested against Gram negative and Gram positive bacteria using agar disc diffusion method.The Gram negative bacteria like Klebsiella pneumonia,Proteus mirabilis,Vibrio cholerae,Salmonella typhi,Escherichia coli and Gram positive bacteria including Staphylococcus aureus,Bacillus subtilis,Enterococcus sp.,Clostridium botulinum(C.botulinum)and Nocardia sp.were selected for analysis.The cultures were obtained from Microbial Culture Maintenances Laboratory,Department of Medical Microbiology,Rajah Muthaiah Medical College,Annamalai University,Tamil Nadu,India.Results:Seven bioactive compounds were detected viz.,phenols,tannin,flavanoids,saponins,terpenes,carbohydrates,alkaloids and steroids.In Gram negative bacteria,Proteus mirabilis treated with ethanol extract of D.olivaceus showed the maximum inhibition zone(17.2 mm),whereas in Gram positive bacteria,C.botulinum treated with diethyl ether extract showed inhibition zone of 19.0 mm.In test for C.vulgaris,Gram negative bacteria Salmonella typhi treated with ethanol extract showed the maximum inhibition zone(13.0 mm),whereas in Gram positive bacteria,C.botulinum treated with chloroform extract of C.vulgaris showed the maximum inhibition zone of 15 mm.Conclusions:The results clearly indicated that D.olivaceus and C.vulgaris contain promising antibacterial compounds.展开更多
基金We thank the National Natural Science Foundation of China (No. 30160097) for financial support.
文摘Two new Steroidal alkaloids, named pengbeisine A and pengbeisine B, were isolated from the fresh bulbs of Fritillaria monatha Migo. along with a known alkaloid. The structures were characterized as 22,26-imino-17, 23-oxido-5α-jerv-6-oxo-2α,3β-diol and 22, 26-imino-17, 23-oxido-5α-jerv-6-oxo-2β,3α-diol on the basis of spectroscopic methods.
基金financially supported by the National Natural Science Foundation of China(No.20872011)
文摘Two new steroidal alkaloids, veraussines A (1) and B (2) were isolated from the roots and rhizomes of Veratrum nigrum var. ussuriense. Their structures were determined as N-(ethoxycarbonyl)- 1 α,2β,3α, 15α-tetrahydroxy-5fl-jervanin- 12-en- 11 -one (1) and N-(methoxycarbonyl)-1α,2β,3α,15α-tetrahydroxy-5/3-jervanin-12-en-1 l-one (2) by spectroscopic analysis.
基金the National Natural Science Foundation of China (32170405)Yunnan Science and Technology Project (202105AE160006,2019FY003004) for partial financial support.
文摘Steroidal alkaloids possess the basic steroidal skeleton with a nitrogen atom in rings or side chains incorporated as an integral part of the molecule.They have demonstrated a wide range of biological activities,and some of them have even been developed as therapeutic drugs,such as abiraterone acetate(Zytiga®),a blockbuster drug,which has been used for the treatment of prostate cancer.Structurally diverse natural steroidal alkaloids present a wide spectrum of biological activities,which are attractive for natural product chemistry and medicinal chemistry communities.This review comprehensively covers the structural classification,isolation and various biological activities of 697 natural steroidal alkaloids discovered from 1926 to October 2021,with 363 references being cited.
文摘A new steroidal alkaloid, vaganine A (1) was isolated together with a known compound paohysandrine A (2) from Sarcooocca vagans. Their structures were determined by spectroscopic evidence.
基金project with finacial support by Chinese National Science Foundation
文摘From the bulbs of Fritillaria siechuanica S.C.Chen cultivated in Sichuan province,a new steroidal alkaloid,siechuansine,was isolated.Spectral methods demonstrated that it is a 13—epimer of wanpeinine A.
基金supported by the Open Fund Project of Hubei Provincial Key Laboratory of Oceurrence and Intervention of Rheumatic Diseases of China (Grant No. OIR 15005:OIR17003A)
文摘Objective: To investigate the effects of steroidal alkaloids and sarcovagine D isolated from Sarcococca hookeriana var. digyna on cell-proliferation and secretion of inflammatory cytokines in TNF-a-induced human RA-FLS MH7 A in vitro, and against complete Freund's adjuvant(CFA)-induced arthritis rats in vivo. Methods: CCK-8 assay was utilized to evaluate the anti-proliferation activity in vitro. In in-vivo study, rats were randomly divided into control group, model group(CFA), steroidal alkaloids(STA) groups(5.0, 2.5 and 1.25 g/kg BW),and sarcovagine D(SD) groups(50, 100 and 200 mg/kg BW), 10 rats for each group. To evaluate the anti-inflammation effect, the histology, biochemical parameters and expression of inflammatory cytokines were detected. Results: Steroidal alkaloids and sarcovagine D showed strong anti-proliferative activity during MH7 A cell culture proliferation and downregulated NO levels, and inflammatory cytokines(IL-1β, IL-6 and PGE_2) in vitro. The rats treated with CFA induced ankle inflammation and lymphocyte infiltration, upregulated IL-1β, TNF-α,IL-6, PGE_2, MDA and NO levels, and downregulated IL-10 and SOD. On the contrary,steroidal alkaloids ameliorated all the changes induced by CFA in rats, suggesting the antiinflammatory effect of steroidal alkaloids and sarcovagine D. Conclusions: The results suggest that Sarcococca hookeriana var. digyna has anti-inflammatory effect. Steroidal alkaloids and sarcovagine D has the potential to cure RA.
文摘Two new D/E trans-5(?)-cevanine alkaloids,Dongbernine and Dongbeirine,have been isolated from Fritillaria thunbergii Miq.var.chekiangensis together with six known compounds,peimine,peiminine,isoverticine,verticindiol,B—sitosterol daucoterol.
文摘The purpose of this study was to isolate a new compound from the seeds of Combretum quadrangulare Kurz, which showed antibacterial activity. The seeds were macerated with 95% of ethanol and then purified by column chromatography using Avicel as the adsorbent. After that, 50% of methanol in water was used as the eluting solvent and continued to further purify by Avicel preparative layer chromatogram 2 times using 80% of methanol in water as developing solvent. MIC of pure compound was done by two fold dilution method. Muller Hinton agar was selected as the media. The new compound was found as combretin, a steroidal alkaloid, and showed antibacterial activity against Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 better than Staphylococcus aureus ATCC 25923.
基金supported by The New Century Talents Scheme of the Ministry of Education (NECT-08-0820)National Innovation Experiment Program for University Students (81061320)
文摘Objective To design and synthesize derivatives of wanpeinine A,the main steroidal alkaloid isolated from the plant Fritillaria shuchengensis,and further study on the structure-activity relationship of the steroidal alkaloid.Methods Acylation and alkylation were used to synthesize the derivatives and their structures were identified via NMR and MS.Results The acylation of wanpeinine A(1) produced 3β,6α-diacetylwanpeinine A(2),3β,6α-dipropionyl-wanpeinine A(3),3β,6α-dichloracetylwanpeinine A(4),3β,6α-dibenzoylwanpeinine A(5),and 3β-methoxy-acylwanpeinine A(6).The alkylation of wanpeinine A formed 3β,6α-dimethoxymethylwanpeinine A(7).Conclusion All compounds are new except for 3β,6α-diacetylwanpeinine A.
基金funded by a grant (No.21ZYJDJC00080) from the Tianjin Committee of Science and Technology of Chinathe National Key Research and Development Project of China (No.2018YFC1707904,2018YFC1707905,and 2018YFC1707403)the Important Drug Development Fund,Ministry of Science and Technology of China (No.2018ZX09735-002).
文摘Solanum lyratum is one of the temperate plants,broadly distributed in Korea,China,Japan,India,and South-East Asia and well-documented in those oriental ethnic medicine systems for curing cancers,jaundice,edema,gonorrhea,cholecystitis,phlogosis,rheumatoid arthritis,etc.This review systematically summarized the research progress on S.lyratum respecting the botany,traditional uses,phytochemistry,pharmacology,and toxicology to increase people’s in-depth understanding of this plant,by data retrieval in a series of online or off-line electronic databases as far as we can reach.Steroidal saponins and alkaloids,terpenoids,nitrogenous compounds,and flavonoid compounds are the main chemical constituents in S.lyratum.Among them,steroidal alkaloids and saponins are the major active ingredi-ents ever found in S.lyratum,exerting activities of anti-cancer,anti-inflammation,anti-microbial,anti-allergy,and anti-oxidation in vivo or in vitro.As a result,S.lyratum has been frequently prescribed for the abovementioned therapeutic purposes,and there are substantial traditional and modern shreds of evidence of its use.
文摘Over the past 30 years,the genus Solanum has received considerable attention in chemical and biological studies.Solanum is the largest genus in the family Solanaceae,comprising of about 2000 species distributed in the subtropical and tropical regions of Africa,Australia,and parts of Asia,e.g.,China,India and Japan.Many of them are economically signifcant species.Previous phytochemical investigations on Solanum species led to the identifcation of steroidal saponins,steroidal alkaloids,terpenes,favonoids,lignans,sterols,phenolic comopunds,coumarins,amongst other compounds.Many species belonging to this genus present huge range of pharmacological activities such as cytotoxicity to diferent tumors as breast cancer(4T1 and EMT),colorectal cancer(HCT116,HT29,and SW480),and prostate cancer(DU145)cell lines.The biological activities have been attributed to a number of steroidal saponins,steroidal alkaloids and phenols.This review features 65 phytochemically studied species of Solanum between 1990 and 2018,fetched from SciFinder,Pubmed,ScienceDirect,Wikipedia and Baidu,using“Solanum”and the species’names as search terms(“all felds”).
基金Supported by University Grants Commission-Rajiv Gandhi National Fellowship(UGC Circular No.F1-17.1/2015-16/RGNF-2015-17-SC-TAM-24190)in January 2016.
文摘Objective:To determine the phytochemicals and screen the antimicrobial potential of Desmococcus olivaceus(D.olivaceus)and Chlorella vulgaris(C.vulgaris)against human bacterial pathogens.Methods:D.olivaceus and C.vulgaris were collected and confirmed through morphological structures.Phytochemicals were analyzed and confirmed by chemical methods.Thin layer chromatography was performed to evaluate the bioactive compounds present in the solvent extracts.Antimicrobial activity of the extract obtained from dried green microalgae was tested against Gram negative and Gram positive bacteria using agar disc diffusion method.The Gram negative bacteria like Klebsiella pneumonia,Proteus mirabilis,Vibrio cholerae,Salmonella typhi,Escherichia coli and Gram positive bacteria including Staphylococcus aureus,Bacillus subtilis,Enterococcus sp.,Clostridium botulinum(C.botulinum)and Nocardia sp.were selected for analysis.The cultures were obtained from Microbial Culture Maintenances Laboratory,Department of Medical Microbiology,Rajah Muthaiah Medical College,Annamalai University,Tamil Nadu,India.Results:Seven bioactive compounds were detected viz.,phenols,tannin,flavanoids,saponins,terpenes,carbohydrates,alkaloids and steroids.In Gram negative bacteria,Proteus mirabilis treated with ethanol extract of D.olivaceus showed the maximum inhibition zone(17.2 mm),whereas in Gram positive bacteria,C.botulinum treated with diethyl ether extract showed inhibition zone of 19.0 mm.In test for C.vulgaris,Gram negative bacteria Salmonella typhi treated with ethanol extract showed the maximum inhibition zone(13.0 mm),whereas in Gram positive bacteria,C.botulinum treated with chloroform extract of C.vulgaris showed the maximum inhibition zone of 15 mm.Conclusions:The results clearly indicated that D.olivaceus and C.vulgaris contain promising antibacterial compounds.