Proteomic Analysis of Stichopus japonicus and Evaluation of the Antiaging Properties of Its Peptide

The sea cucumber Stichopus japonicus is one of the"Eight Treasures of Seafood"and contains a number of bioactive components involved in multiple physiological and pharmacological functions.Proteins and peptides are generally considered to be responsible for these beneficial properties.In this study,a total of 3478 proteins and 17390 peptides were identified in Stichopus japonicus by proteomics methods.Among them,4 proteins were involved in 8 metabolic pathways,especially oxidative phosphorylation and cell senescence.Subsequently,lifespan assay and oxidative stress test were performed to investigate the peptides prepared from sea cucumber protein hydrolyzate using the aging model of Caenorhabditis elegans.The results of the anti-aging experiment demonstrated that high-dose peptides significantly prolonged the lifespan of nematodes(30.50%),and improved their capacity to inhibit oxidative stress.The results provide evidence supporting the development of bioactive proteins and peptides derived from Stichopus japonicus as functional foods and lay the foundation for the research of an anti-aging drug.

Effects of Different Drying Processes on the Effective Components of Stichopus japonicus Viscera

Drying is a basic link in seafood processing, and the effects of forced air drying and vacuum drying on the effective components of Stichopus japonicus viscera were compared with the moisture, total saponin and polysaccharide contents as the detection indexes. The contents of effective components obtained using forced air drying were slightly lower than those obtained using vacuum drying, but the forced air drying method used short drying time and low economic energy consumption. Excellent drying effects and low cost of forced air drying made it can be adapted to the requirements of large-scale production applications.

海湾扇贝、刺参、魁蚶间作立体混养模式试验

为充分利用水体空间,降低养殖成本,在海洋牧场海域进行了海湾扇贝(Argopecten irradians)、刺参(Stichopus japonicus)、魁蚶(Scapharca broughtonii)间作立体混养试验,形成由不同营养级生物组成的综合养殖系统。苗种增殖后对试验海域和非试验海域进行了水质监测,结果显示,苗种增殖对试验海域没有造成污染,而三个水产品种的滤食特性对水质有一定的改善。统计三个水产品种收获情况,海湾扇贝、刺参和魁蚶产量分别为11000 kg/hm^(2)、767.59 kg/hm^(2)和1302 kg/hm^(2)。试验证明,海湾扇贝、刺参、魁蚶间作立体混养模式不仅具有较好的生态效益,同时具有显著的经济效益。

刺参-单环刺螠池塘生态健康养殖技术

刺参(Stichopus japonicus)与单环刺螠(Urechis unicinctus)池塘生态混养试验中,池塘进、排水口COD、磷酸盐、总磷、氨氮、亚硝酸盐、硝酸盐和总氮含量对比表明:池塘排水口的COD、总氮、氨氮较进水口有明显的降低,生态效益明显。该养殖模式每公顷生产刺参和单环刺螠合计4950 kg,产值65.25万元,纯利润35.25万元,经济效益显著。

马粪海胆与刺参自然海区网箱混养技术

2019年3月,在大连近海内湾海域投放3 m×3 m×3 m网箱进行马粪海胆(Hemicentrotus pulcherrimus)与刺参(Stichopus japonicus)的混养试验,到2020年5月陆续投放400多个网箱,并配套设置海藻养殖台筏,到2021年9月,马粪海胆产量200~250 kg/箱,成活率70%,总产值达到4万元/箱以上,净利润2.5万~2.8万元/箱,经济效益和社会效益显著。

刺参海水网箱生态育苗技术

刺参(Stichopus japonicus)是中国重要的水产养殖品种之一,其苗种需求持续增长。文章介绍了刺参海水网箱生态育苗技术,包括海区选择、网箱结构与设置、苗种繁育及放养、生理生态学指标监测、日常管理和参苗出箱等,供广大养殖者参考。