[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as p...[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as parents to construct F1 orthogonal group. A total of 138 FI individuals were selected as experimental materi- als for construction of color around the stone gene pool （B1） and non-color around the stone gene pool （B2） by using bulked segregant analysis （BSA） method, molec- ular markers linked to the flesh color around the stone of P. persica （L.） Batsch were selected with SSR molecular marker technology. [Result] After selection with 256 pairs of SSR primers, three pairs of molecular markers linked to the gene con- trolling flesh color around the stone of P. persica （L.） Batsch were selected （UDP96- 003, ch04g09 and UDP97-402）. In addition, genetic distances between the three molecular markers and the gene controlling flesh color around the stone of P. persi- ca （L.） Batsch were calculated, which were 16.7, 10.1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.展开更多
Objective:Assessment of the relationship between stone location,composition,color,size and impaction with success rate of pneumatic ureterolithotripsy.Methods:This study was performed on 440 patients who were candidat...Objective:Assessment of the relationship between stone location,composition,color,size and impaction with success rate of pneumatic ureterolithotripsy.Methods:This study was performed on 440 patients who were candidate for pneumatic ureterolithotripsy admitted in an academic urology department from February 2004 to June 2006.Exclusion criteria included active urinary tract infection and pregnancy.Information such as stone composition,color,impaction,size,location and surface were recorded.Success rate was defined as stone fragmentation to < 2 mm.We used chi-square test and student t-test for statistical analysis.Results:The success rate of pneumatic ureterolithotripsy was 83.0 %.Mean stone size was 9.86 ± 3.79 mm.The stone free rates in upper,middle and lower ureter were 66.7 %,100 % and 90.7 % respectively(P<0.000 1).Stone free rate was 100 % and 69.35 % in <5 mm and >5 mm stones respectively(P<0.000 1).Impaction did not correlate significantly with stone free rate(P=0.17).The success rate was 100 % in phosphate and cystine stones.There was a significant statistical difference between success rate and stone composition(P=0.026).The most common fragmented stone color was gold(95.5 %).Stone color correlated significantly with stone free rate(P<0.000 1).Conclusion:In this series ureterolithotripsy,stone free rate had a significant correlation with stone color,size,composition and location,but it was independent of stone impaction.展开更多
基金Supported by Fund of Hebei Academy of Agriculture and Forestry Sciences(A06120203)~~
文摘[Objective] This study aimed to select SSR molecular markers linked to flesh color around the stone of Prunus persica （L.） Batsch. [Method] P. persica （L.） Batsch varieties Chongyanghong and Yanhong were used as parents to construct F1 orthogonal group. A total of 138 FI individuals were selected as experimental materi- als for construction of color around the stone gene pool （B1） and non-color around the stone gene pool （B2） by using bulked segregant analysis （BSA） method, molec- ular markers linked to the flesh color around the stone of P. persica （L.） Batsch were selected with SSR molecular marker technology. [Result] After selection with 256 pairs of SSR primers, three pairs of molecular markers linked to the gene con- trolling flesh color around the stone of P. persica （L.） Batsch were selected （UDP96- 003, ch04g09 and UDP97-402）. In addition, genetic distances between the three molecular markers and the gene controlling flesh color around the stone of P. persi- ca （L.） Batsch were calculated, which were 16.7, 10.1 and 17.0 cM, respectively. [Conclusion] This study laid the foundation for further selection of co-dominant molecular markers with closer genetic distance.
文摘Objective:Assessment of the relationship between stone location,composition,color,size and impaction with success rate of pneumatic ureterolithotripsy.Methods:This study was performed on 440 patients who were candidate for pneumatic ureterolithotripsy admitted in an academic urology department from February 2004 to June 2006.Exclusion criteria included active urinary tract infection and pregnancy.Information such as stone composition,color,impaction,size,location and surface were recorded.Success rate was defined as stone fragmentation to < 2 mm.We used chi-square test and student t-test for statistical analysis.Results:The success rate of pneumatic ureterolithotripsy was 83.0 %.Mean stone size was 9.86 ± 3.79 mm.The stone free rates in upper,middle and lower ureter were 66.7 %,100 % and 90.7 % respectively(P<0.000 1).Stone free rate was 100 % and 69.35 % in <5 mm and >5 mm stones respectively(P<0.000 1).Impaction did not correlate significantly with stone free rate(P=0.17).The success rate was 100 % in phosphate and cystine stones.There was a significant statistical difference between success rate and stone composition(P=0.026).The most common fragmented stone color was gold(95.5 %).Stone color correlated significantly with stone free rate(P<0.000 1).Conclusion:In this series ureterolithotripsy,stone free rate had a significant correlation with stone color,size,composition and location,but it was independent of stone impaction.
