Stool antigen tests for the management of Helicobacter pylori infection

Stool antigen tests(SATs)are noninvasive diagnostic modules for Helicobacter pylori(H.pylori)infection.Two types of SATs exist for the diagnosis of H.pylori infection,one based on enzyme immunoassay(EIA)and another on immunochromatography(ICA).SATs do not require expensive chemical agents or specified equipment;hence,they are less expensive compared with the urea breath test.Both European and Japanese guidelines have shown that EIA-based SATs using monoclonal antibodies are useful for primary diagnosis as well as for the assessment of eradication therapy.ICA-based tests do not require particular equipment and are therefore useful in developing countries.SATs are also useful for the diagnosis of H.pylori infection in children and post gastric surgery patients.SATs performed via EIA can assess H.pylori infection in a large number of subjects,almost as well as serology.Thus,SATs would be useful or detecting current infection in such a survey to identify and eradicate H.pylori infection.The accuracy of SATs is lower when the stool samples are unformed or watery,because H.pylorispecific antigens in the stool samples are diluted.Temperature and the interval between stool sample collection and measurement also affect the results of SATs.The choice of test kit depends on the sensitivity and specificity in each region and the circumstances of each patient.

Influence of proton pump inhibitor treatment on Helicobacter pylori stool antigen test

AIM: To investigate the effects of proton pump inhibitor (PPI) treatment on stool antigen test using the TestMate pylori enzyme immunoassay. METHODS: This study assessed 28 patients [16 men and 12 women; mean age (63.1 ± 5.9) years; range, 25-84 years] who underwent stool antigen test and urea breath test (UBT) before and after PPI administration. RESULTS: Using the UBT as the standard, the sensitivity, specif icity and agreement of the stool antigen test in all 28 patients were 95.2%, 71.4%, and 89.3%, respectively, before PPI administration, and 88.9%, 90.9%, and 89.3%, respectively, after PPI treatment. Mean UBT values were 23.98% ± 5.33% before and 16.19% ± 4.75% after PPI treatment and, in 15 patients treated for ≥ 4 wk, were signif icantly lower after than before 4 wk of PPI treatment (12.58% ± 4.49% vs 24.53% ± 8.53%, P = 0.048). The mean optical density (A450/630) ratios on the stool antigen test were 1.16 ± 0.20 before and 1.17 ± 0.24 after PPI treatment (P = 0.989), and were 1.02 ± 0.26 and 0.69 ± 0.28, respectively, in the group treated for > 4 wk (P = 0.099).

Comparison of invasive methods and two different stool antigen tests for diagnosis of H pylori infection in patients with gastric bleeding

瞄准：为了比较二不同 H pylori 凳子抗原，作为非侵略的诊断方法测试。方法：学习人口由为病人放血的 22 上面的胃肠的系统组成了。脲呼吸测试(UBT ) ，快速的 urease (车辙) 和组织病理学说的检查被用于所有病人。从这些病人的凳子标本被快速的长带检验 ! 为 H pylori 抗原的察觉的 HpSA 和 HpSA 步骤 simple H pylori 抗原盒子测试。结果：为这 22 个病人，(68.2%) 15 作为积极被诊断，(31.8%) 七是为由标准答案方法的 H pylori 感染的诊断 negative。而(45.5%) 10 是积极的，(54.5%) 12 被诊断由快速的长带否定 ! HpSA 测试。敏感，特性，积极、否定的预兆的价值分别地是 60% ， 86% ， 90% 和 50% 。什么时候与标准答案方法相比，这些差别不是重要的。然而，六个病人(27.3%) 是积极的，并且(72.7%) 16 由简单 H pylori 凳子抗原盒子测试是否定的。敏感，特性，积极、否定的预兆的价值分别地是 33% ， 86% ， 83% 和 38% 。比作标准答案方法，简单 H pylori 凳子抗原盒子测试结果是显著地不同的(P = 0.012 ) 。结论：快速的长带 ! HpSA 测试能在微生物学实验室作为一个平淡的诊断工具被使用在上面的胃肠的系统流血估计 H pylori 的临床的意义和根除控制病人。

Detection of H.pylori Antigen in the Stool Samples of Children,in Tekirdag,Turkey

Introduction: Helicobacter pylori (H. pylori) is an important bacterial pathogen, predominantly in undeveloped countries and regions. We conducted this study to investigate the presence of H. pylori antigen in the stool specimens of children, in Tekirdag, Turkey. Material and Methods: Stool specimens of 1441 children aging between 0 month to 15 years, with the complaints of abdominal pain, anemia, gastroenteritis, nausea and vomiting were included in the study. The subjects were divided into three groups based on their ages: Group A (0 month - 5 years), Group B (6 - 10 years) and Group C (11 - 15 years). All specimens were tested for H. pylori antigen with HP Ag (DIA.PRO, Diagnostic Bioprobes, Italy). Results were evaluated statistically. Results: The median age of children with H. pylori positive samples was 6.6 ± 4.1 and 47.9% were girls. Overall positivity of H. pylori stool antigen was 6.6%. Positivity rates due to ages in Groups A, B and C were: 8.9%, 5.8%,? 5.2% respectively (x2 = 9.3, p = 0.01). No difference was observed due to gender (x2 = 0.17, p = 0.68). Conclusion: Higher antigen positivity obtained in 0 - 5 years group, indicates the risk of acquiring the infection in early years of life. Preventive measures for the development of H. pylori infection gain importance, especially in developing countries.

Comparison of a monoclonal antigen stool test (Hp StAR) with the ^(13)C-urea breath test in monitoring Helicobacter pylori eradication therapy

AIM: To evaluate the agreement between a mAb-based stool test (HP StAR) and the urea breath test (UBT) in monitoring (H pylori) infection after eradication therapy.METHODS: Patients with discordant results on UBT and Hp StAR underwent endoscopy with biopsies for rapid urease test, culture, and histology to confirm H pylori status.RESULTS: Among 250 patients (50±14 years), 240 (96.0%) had concordant UBT and Hp StAR tests with a significant correlation between DOB and A values (R = 0.87; P＜0.0001).The remaining 10 (4.0%) patients had discordant tests (positive Hp StAR and negative UBT) with the Hp StAR inaccurate in five cases (false positive) and UBT inaccurate in the other five cases (false negative). The 'maximal expected' sensitivity, specificity, +PV, -PV, +LR, and -LR were 91%, 100%, 100%, 97.4%, ∞, and 8.2 respectively,for the UBT, and 100%, 97.4%, 91%, 100%, 38.8, and 0,respectively, for the Hp StAR. Overall accuracy for both tests was 98%.CONCLUSION: Both the UBT and the Hp StAR are equally accurate in monitoring H pylori infection. Nowadays,the choice of the 'best' non-invasive H pylori test in the post-treatment setting should be done not only in terms of diagnostic accuracy but also in view of cost and local facilities.

Retrospective analysis of discordant results between histology and other clinical diagnostic tests on helicobacter pylori infection

BACKGROUND A reliable test is essential for diagnosing Helicobacter pylori(H.pylori)infection,and crucial for managing H.pylori-related diseases.Serving as an excellent method for detecting H.pylori infection,histologic examination is a test that clinicians heavily rely on,especially when complemented with immunohistochemistry(IHC).Additionally,other diagnostic tests for H.pylori,such as the rapid urease test(CLO test)and stool antigen test(SA),are also highly sensitive and specific.Typically,the results of histology and other tests align with each other.However,on rare occasions,discrepancy between histopathology and other H.pylori diagnostic tests occurs.AIM To investigate the discordance between histology and other H.pylori tests,the underlying causes,and the impact on clinical management.METHODS Pathology reports of gastric biopsies were retrieved spanning August 2013 and July 2018.Reports were included in the study only if there were other H.pylori tests within seven days of the biopsy.These additional tests include CLO test,SA,and H.pylori culture.Concordance between histopathology and other tests was determined based on the consistency of results.In instances where histology results were negative while other tests were positive,the slides were retrieved for re-assessment,and the clinical chart was reviewed.RESULTS Of 1396 pathology reports were identified,each accompanied by one additional H.pylori test.The concordance rates in detecting H.pylori infection between biopsy and other tests did not exhibit significant differences based on the number of biopsy fragments.117 discrepant cases were identified.Only 20 cases(9 with CLO test and 11 with SA)had negative biopsy but positive results in other tests.Four cases initially stained with Warthin-Starry turned out to be positive for H.pylori with subsequent IHC staining.Among the remaining 16 true discrepant cases,10 patients were on proton pump inhibitors before the biopsy and/or other tests.Most patients underwent treatment,except for two who were untreated,and two patients who were lost to follow-up.CONCLUSION There are rare discrepant cases with negative biopsy but positive in SA or CLO test.Various factors may contribute to this inconsistency.Most patients in such cases had undergone treatment.

体检人群幽门螺杆菌感染检测意愿调查

目的探讨体检人群对H.pylori感染检测的主动性及其对检测方式的倾向性。方法于2022年9月至2022年12月在国内两家综合性三甲医院的体检人员中发放电子调查问卷,问卷内容包括受访者对H.pylori感染及检测方式的了解情况,主动给自己及家中老人、儿童(<14岁)检测的意愿,及其对尿素呼气试验(urea breath test,UBT)和H.pylori粪便抗原试验(H.pylori stool antigen test,HpSAT)的比较与选择等。结果共回收488份有效问卷。消化道症状或疾病在体检人群(57.0%)及其家庭成员(35.5%)中普遍存在。体检人群对H.pylori感染的熟悉率仅为11.9%。43.0%受访者会在体检时主动检测,36.9%的受访者在医师建议下才会进行检测,而20.1%不会主动检测。影响检测主动性的因素主要是对H.pylori感染的了解程度。对于家中老人和儿童的检测,大部分体检人员需要医师建议才会进行检测。体检人员选择检测方式时,主要看其重准确性、方便性、安全性,超过80%体检人员会选择UBT。结论体检人群对H.pylori感染的认知水平较低,但检测意愿性较强,在检测时倾向于选择传统的UBT,对新兴的快速HpSAT缺乏了解。

幽门螺杆菌粪便抗原检测法在胃肠疾病中的临床诊断价值研究

目的研究幽门螺杆菌粪便抗原(HpSA)检测法在胃肠疾病中的临床诊断价值。方法196例胃肠疾病患者,采集患者的粪便样本以及胃窦区黏膜样本,分别开展HpSA检测法、快速尿素酶试验(RUT)联合组织学检测法。以RUT联合组织学检测结果为金标准,分析HpSA检测法的诊断效能。结果196例患者经RUT联合组织学检测法确诊123例患者感染幽门螺杆菌(Hp),73例未感染Hp,阳性率为62.76%(123/196)。以RUT联合组织学检测法为金标准,HpSA检测法检出阳性患者123例,阳性检出率为62.76%(123/196),灵敏度为97.56%(120/123)、特异度为95.89%(70/73)、阳性预测值为97.56%(120/123)、阴性预测值为95.89%(70/73)、诊断准确率为96.94%(190/196)。结论HpSA检测法在胃肠疾病中的诊断准确率、灵敏度和特异度均较高,可以将该诊断方法应用于儿童、老年人、胃癌家属的筛查中,同时对于不能进行13C-尿素呼气试验(UBT)或14C-UBT、组织学诊断的患者或地区也可以使用该方法。

血清癌胚抗原、糖类抗原199、糖类抗原125配合粪便隐血试验在结直肠癌早期筛查中的应用

目的:探讨血清癌胚抗原(CEA)、糖类抗原199(CA199)、糖类抗原125(CA125)联合粪便隐血试验检测对早期诊断结直肠癌的价值。方法:选取100例结直肠癌患者为观察组,另选取100例同期健康体检者为对照组,检测两组各指标水平;比较三种肿瘤标志物与粪便隐血试验联合检测阳性率与单项检测阳性率;对比观察组不同TNM分期者的各项检测指标水平,并对患者经6个月治疗后复发转移与无复发转移者的检测结果比较。结果:观察组各项检测结果均显著高于对照组,差异有统计学意义(P<0.05);联合检测的阳性检出率明显高于单项检测,差异有统计学意义(P<0.05);观察组患者随着TNM分期的升高在各项检测结果上也有所升高,观察组患者经6个月的治疗后,复发转移患者在各项检测结果上均高于无复发转移患者,差异有统计学意义(P<0.05)。结论:血清CEA、CA199、CA125与粪便隐血试验结直肠癌的早期筛查及诊断有重要意义,联合检测有利于疾病的早诊断,评估治疗效果,并且判断预后。

幽门螺杆菌粪便抗原检测──一种新的非侵入性诊断方法

评估幽门螺杆菌粪便抗原（ＨｐＳＡ）检测诊断ＨＰ感染的可靠性。方法：收集４３例接受胃镜检查患者的粪 便标本，用一种市售的ＥＬＩＳＡ试剂盒检测ＨｐＳＡ；以尿素酶试验、培养和涂片染色检测Ｈｐ作为“金标准”，培养和／ 或涂片染色阳性定为Ｈｐ感染。结果：“金标准”诊断Ｈｐ阳性２４例，阴性１９例。“金标准”阳性的２４例ＨｐＳＡ检测均阳 性，阴性的１９例ＨｐＳＡ检测１８例阴性和１例阳性。ＨｐＳＡ试验的敏感性为１００％（２４／２４），特异性为９４．７％（１８／１９），总的 诊断准确率为９７．６％（４２／４３）。结论：ＨｐＳＡ试验是一简便、非侵入性、准确诊断Ｈｐ感染的新方法。

幽门螺杆菌粪便抗原和血清抗体胶体金试验联合检测在体检人群的应用价值

目的了解体检人群幽门螺杆菌（HP）感染情况，探讨粪便HP抗原和血清HP抗体胶体金试验联合检测体检人群HP感染的应用价值。方法采用14C-尿素呼气试验（14C—UBT）、粪便HP抗原及血清HP抗体胶体金试验三种方法对478名体检者进行HP检浸4并进行统计分析。结果三种方法检测478名体检者的HP阳性率分剐为43．7％，43．5％，45．6％，三种方法之问及男女性别之间的差异无统计学意义（P〉0．05），其中血清HP抗体胶体金试验的HP既往感染率为7．32％（35／478），粪便HP抗原与血清HP抗体胶体金试验联合检测体检人群既可以明确诊断现症感染又可以了解既往感染史。结论体检人群HP感染率低于慢性活动性胃炎、消化性溃疡病人的HP感染率；粪便HP抗原和血清HP抗体肢体金试验联合检测可以应用于普通人群的筛查，能同时反映其临床和流行病学意义。

1760例婴幼儿腹泻中轮状病毒检出率及临床意义

目的了解沙井地区婴幼儿腹泻中轮状病毒(RV)感染现状,为临床诊断和治疗提供可靠依据。方法采用胶体金法对1760例来我院就诊的腹泻患儿的粪便标本进行RV抗原检测,并比较检测结果。结果1760例腹泻标本中有852例RV结果阳性,阳性率为48.41%;其中0.5-1岁组腹泻患儿阳性率可达69.96%;以每年11、12月份为发病高峰。结论轮状病毒是本地区婴幼儿腹泻的主要病原,且有明显的季节性。

幽门螺杆菌粪便抗原纳米金侧流免疫层析快速检测板的研制及应用评价

目的 研制幽门螺杆菌（HP）粪便抗原（HPSA）纳米金侧流免疫层析快速检测板.方法 利用枸橼酸钠作为还原剂制备纳米金,标记兔抗HP多价抗体,建立双抗体夹心法的纳米金侧流免疫层析快速检测板;采用该检测板和英国Atlas公司试纸条分别对HP阳性病例组（123例）、健康体检HP阳性对照I组（109例）、健康体检HP阴性对照II组（129例）进行双盲法HPSA检测,并以14C-尿素呼气试验（14C-UBT）为金标准,对结果进行评价.结果 制备的直径40 nm纳米金最大吸收波长（λmax）为525 nm,标记抗体的0.05 mol/L硼酸盐缓冲液最适pH为7.6,抗体标记最佳用量为18 μg/ml（最小标记量为15 μg/ml）;检测板与试纸条的检测结果在三组之间差异无统计学意义（χ^2=0～0.337,P均〈0.05）;以14C-UBT结果为金标准,检测板的灵敏度、特异度、符合率分别为98.3%,99.2%和98.6%,试纸条的灵敏度、特异度、符合率分别为97.4%,98.4%和97.8%.结论 以纳米金标记多抗的双抗体夹心法侧流免疫层析快速检测板,检测HPSA诊断HP感染,方法简便、价格便宜、重复性好、无创伤,具有很高的敏感度和特异度.

粪便抗原检测临床诊断幽门螺杆菌感染的可靠性研究

背景:我国属于幽门螺杆菌(Hp)感染高发国家。在众多Hp感染检测方法中,Hp粪便抗原(HpS A)检测具有非侵入性、敏感性和特异性较高、操作简便、价格低廉等优势。目的:评价HpS A检测试剂盒临床诊断Hp感染的可靠性。方法:340例行13C-尿素呼气试验(13C-UBT)的受试者同时留取粪便标本,以待评估的酶联免疫法HpS A检测试剂盒检测Hp感染。以13C-UBT为金标准,计算待评估试剂盒诊断Hp感染的阳性符合率、阴性符合率、总符合率和κ系数。结果:与13C-UBT结果比对,待评估HpS A检测试剂盒诊断Hp感染的阳性符合率为96.3%,阴性符合率为75.8%,总符合率为87.4%,κ系数为0.737 522。结论:待评估的HpS A检测试剂盒用于临床诊断Hp感染,与金标准13C-UBT具有较高的符合率和一致性,结果可靠,可在临床上推广应用。

幽门螺杆菌粪便抗原检测法在幽门螺杆菌根除后患者中的效果

目的探讨幽门螺杆菌(Hp)粪便抗原检测(HpSA)在Hp根除后患者中的效果,为Hp感染患者后期复查提供依据。方法选取该院2011年12月至2013年12月进行Hp根治后的170例患者进行研究,所有患者均进行14C尿素呼气试验检测HpSA,观察HpSA检测Hp的灵敏度和准确率,为Hp感染的检测提供依据。结果与14C尿素呼气试验金标准检测比较,患者治疗1周后,HpSA试验检测灵敏度为78.08%,诊断正确率为68.24%;患者治疗2周后HpSA试验检测灵敏度为74.12%,诊断正确率为54.71%;患者治疗3周后,HpSA试验检测灵敏度为46.38%,诊断正确率为47.65%。结论 HpSA检测方法的诊断准确率高、灵敏度高、特异度高、适用于Hp的诊断,尤其适用于儿童、老年患者的筛查及无法开展13C或14C-尿素呼气试验的贫困地区,且检测值不受患者是否进行治疗的影响。

幽门螺杆菌粪便抗原检测在儿童中的应用价值研究

目的 :对幽门螺杆菌粪便抗原 (Helicobacterpyloristoolantigen ,HpSA)检测诊断儿童Hp感染的应用价值进行探讨。方法 :以快速尿素酶、组织学和细菌培养作为诊断Hp的金标准 (快速尿素酶试验、组织学和细菌培养其中 2项阳性则判定为Hp阳性 ,细菌培养 1项为阳性也判定为Bp阳性 ) ,采用ELISA法检测 4 0例因上消化道症状接受胃镜检查患儿的HpSA ,评价HpSA检测诊断儿童Hp感染的敏感性、特异性。同时在 2 9例患儿中以13 C -UBT作对照 ,评价HpSA检测诊断Hp感染的准确性。结果 :在 4 0例患儿中 ,HpSA检测的敏感性、特异性和准确性分别 94 .4 4 % ,90 .91%和 92 .5 0 % ;在 2 9例患儿中以13 C -UBT作标准 ,HpSA检测诊断Hp感染敏感性、特异性和准确性分别为 91.6 7% ,88.2 3%和 89.6 6 %。结论 :HpSA检测是一种方便、简单、准确的诊断儿童Hp感染的方法。

粪便幽门螺杆菌抗原检测对儿童复发性腹痛病因诊断的价值

目的慢性胃炎、消化性溃疡是引起儿童复发性腹痛(recurrent abdom inalpain,RAP)的主要原因之一,而幽门螺杆菌(helicobacterpylori,Hp)感染与慢性胃炎、消化性溃疡的相关性尚有待进一步探讨。目前国内儿科临床上缺乏一种简便、安全、经济、有效地非侵入性Hp感染检测方法。故此,该研究探讨RAP与Hp感染的关系及幽门螺杆菌粪便抗原检测(Hpstoolantigen,HpSA)在RAP病因诊断中的价值。方法对182例RAP患儿进行13C尿素呼气试验(13Cureabreathtest,13CUBT)及HpSA检测,测定其Hp感染率,观察抗Hp治疗后RAP患儿的腹痛缓解率,并对两种检测方法进行比较。结果RAP儿童Hp感染率为41.2%。男女比例为1∶1.143,差异无显著性。Hp阳性儿童进行抗Hp治疗后,Hp根除和未根除的患儿腹痛缓解率分别为93.4%及28.6%。两者比较差异有显著性(P<0.05)。以13CUBT作为金标准计算,HpSA检测方法的敏感性、特异性、假阳性率、假阴性率、准确率、阳性预测值、阴性预测值分别为90.7%,97.2%,2.8%,9.3%,94.5%,95.8%,93.7%。HpSA试验与13CUBT一致性用Kappa值表示为0.886,U=25.237,P=0,两者有高度一致性。结论Hp感染与RAP有密切相关性,可能为RAP的主要致病因素;HpSA检测有较高的敏感性和特异性,还具有安全、简便可靠、经济、非侵入性等优点,在RAP病因诊断中具有良好的实用价值。

粪便抗原检测儿童及青少年幽门螺杆菌感染诊断试验的系统评价

目的:采用Meta分析的方法评价幽门螺杆菌粪便抗原(H pylori stool antigen,HpSA)诊断儿童H pylori感染的价值,为临床应用提供依据.方法:以"幽门螺杆菌"、"儿童""诊断性试验"、"粪便抗原"、"诊断准确性"为检索词,通过联机检索CNKI中国期刊全文数据库、CBM中国生物医学文献数据库,文献追溯和手工检索的方法收集发表时间为1998-01/2008-05国内正式刊物上公开发表的有关评价粪便抗原检测H pylori感染准确性的相关研究文献.按确定的纳入、排除标准进行筛选,采用QUADAS工具对纳入的文献进行质量评价,应用MetaDisc1.4软件进行Meta分析,综合评价灵敏度、特异度、似然比、诊断优势比和SROC曲线下面积、Q*指数等指标.结果:共17篇文献符合纳入标准,各研究间具有同质性.共累计样本含量1466例,由金标准确诊H pylori感染737例,非感染729例.Meta分析结果显示各研究之间的一致性较高,粪便抗原诊断H pylori感染的合并敏感度、特异度分别是92%(95%CI90%-94%)和92%(95%CI90%-94%);合并阳性似然比、阴性似然比分别为11.44(95%CI8.83-14.81)和0.10(95%CI0.08-0.13).合并诊断优势比DOR126.14(84.84-187.55).绘制SROC曲线,估计SROC曲线下面积AUC=0.9696,Q*=0.9193.结论:粪便抗原检测儿童及青少年Hpylori感染的方法准确度较高.

2种非侵入性检测方法对幽门螺杆菌感染的诊断价值

目的探讨粪便Hp抗原(HpSA)及14 C-尿素呼气试验(14 C-UBT)2种非侵入性检测方法对幽门螺杆菌(Hp)感染的诊断价值。方法采用胃黏膜快速尿素酶法(RUT)和活组织切片染色法联合检测的结果作为Hp感染诊断的"金标准",对2009年1月-2011年6月在新疆医科大学附属中医医院门诊就诊的因上消化道症状接受胃镜检查的132例患者进行粪便Hp抗原(HpSA)及14 C-尿素呼气试验(14 C-UBT)的检测,并与"金标准"进行对比分析。结果 132例患者中RUT法检出Hp阳性95例,组织切片染色法检出Hp阳性92例,其中81例2种方法检测均阳性,将此81例患者作为Hp感染的现症患者,26例2种方法均阴性者作为Hp非感染者。对"金标准"检测的81例Hp阳性患者及26例阴性患者再行HpSA及14 C-UBT检测。以"金标准"作为Hp感染的诊断标准,14 C-UBT检测的敏感性为95.06%(77/81),特异性为92.31%(24/26),阳性预测值为97.47%(77/79),阴性预测值为85.71%(24/28),准确性为94.40%(101/107);HpSA检测的敏感性为91.36%(74/81),特异性为88.46%(23/26),阳性预测值为96.10%(74/77),阴性预测值为76.67%(23/30),准确性为90.65%(97/107)。结论 HpSA及14 C-UBT检测是非侵入性诊断Hp感染的较理想的方法,值得在临床推广,临床医生可根据具体情况选择检测方法,也可以联合运用检测方法来减少假阴性或假阳性结果的出现。

幽门螺杆菌粪便抗原检测——一种新的非侵入性检查

目的:了解幽门螺杆菌粪便抗原检测方法(Helicobacter pylori stool Antigen HpSA)的准确性与可靠性。方法:采用酶联免疫分析“双抗体夹心法”对303例因上消化道症状而进行胃镜检查的患者的粪便标本进行幽门螺杆菌检测,以快速尿素酶试验,组织学染色和培养检测Hp,以三项检查中至少两项阳性或Hp培养检测阳性者判定为Hp感染作为“金标准”。结果;“金标准”诊断Hp感染阳性182例,阴性121例。“金标准”阳性的182例中176例HpSA检测阳性,阴性的121例中100例HpSA检测阴性,HpSA敏感度为96.7％(176/182),特异度为82.6％(100/121)。结论:HpSA检测是一种简便易行,准确性高的非侵入性诊断Hp的方法,值得临床进一步推广。