期刊文献+
共找到16,122篇文章
< 1 2 250 >
每页显示 20 50 100
CARD6在脓毒症心肌损伤中的机制研究
1
作者 李一民 石宁宁 +2 位作者 曲央旺姆 辛勇 周吴刚 《上海医药》 CAS 2024年第5期39-42,69,共5页
目的:探讨CARD6在脓毒症心肌损伤中的调节机制。方法:将C57BL/6小鼠随机分为对照组与模型组。采用LPS腹腔注射法构建脓毒症心肌损伤小鼠模型,RT-qPCR检测小鼠心肌组织中CARD6、caspase-1的mRNA水平。体外实验则采用LPS刺激转染CARD6过... 目的:探讨CARD6在脓毒症心肌损伤中的调节机制。方法:将C57BL/6小鼠随机分为对照组与模型组。采用LPS腹腔注射法构建脓毒症心肌损伤小鼠模型,RT-qPCR检测小鼠心肌组织中CARD6、caspase-1的mRNA水平。体外实验则采用LPS刺激转染CARD6过表达质粒的H9C2细胞,检测caspase-1、细胞活力、CK-MB水平。结果:与对照组相比,模型组小鼠心肌组织中LDH、CK-MB含量,caspase-1表达水平上升,提示脓毒症导致小鼠心肌损伤,而CARD6表达水平明显降低。体外实验发现CARD6表达水平呈下降趋势,转染含有CARD6的过表达质粒后细胞释放CK-MB的水平降低、细胞活力增加、caspase-1水平降低。结论:CARD6可改善脓毒症所致心肌损伤,其机制可能与caspase-1介导的细胞焦亡相关。 展开更多
关键词 card6 CASPASE-1 细胞焦亡 脓毒症 心肌损伤
下载PDF
Credit Card Fraud Detection Using Improved Deep Learning Models
2
作者 Sumaya S.Sulaiman Ibraheem Nadher Sarab M.Hameed 《Computers, Materials & Continua》 SCIE EI 2024年第1期1049-1069,共21页
Fraud of credit cards is a major issue for financial organizations and individuals.As fraudulent actions become more complex,a demand for better fraud detection systems is rising.Deep learning approaches have shown pr... Fraud of credit cards is a major issue for financial organizations and individuals.As fraudulent actions become more complex,a demand for better fraud detection systems is rising.Deep learning approaches have shown promise in several fields,including detecting credit card fraud.However,the efficacy of these models is heavily dependent on the careful selection of appropriate hyperparameters.This paper introduces models that integrate deep learning models with hyperparameter tuning techniques to learn the patterns and relationships within credit card transaction data,thereby improving fraud detection.Three deep learning models:AutoEncoder(AE),Convolution Neural Network(CNN),and Long Short-Term Memory(LSTM)are proposed to investigate how hyperparameter adjustment impacts the efficacy of deep learning models used to identify credit card fraud.The experiments conducted on a European credit card fraud dataset using different hyperparameters and three deep learning models demonstrate that the proposed models achieve a tradeoff between detection rate and precision,leading these models to be effective in accurately predicting credit card fraud.The results demonstrate that LSTM significantly outperformed AE and CNN in terms of accuracy(99.2%),detection rate(93.3%),and area under the curve(96.3%).These proposed models have surpassed those of existing studies and are expected to make a significant contribution to the field of credit card fraud detection. 展开更多
关键词 card fraud detection hyperparameter tuning deep learning autoencoder convolution neural network long short-term memory RESAMPLING
下载PDF
A Self-Adapting and Efficient Dandelion Algorithm and Its Application to Feature Selection for Credit Card Fraud Detection
3
作者 Honghao Zhu MengChu Zhou +1 位作者 Yu Xie Aiiad Albeshri 《IEEE/CAA Journal of Automatica Sinica》 SCIE EI CSCD 2024年第2期377-390,共14页
A dandelion algorithm(DA) is a recently developed intelligent optimization algorithm for function optimization problems. Many of its parameters need to be set by experience in DA,which might not be appropriate for all... A dandelion algorithm(DA) is a recently developed intelligent optimization algorithm for function optimization problems. Many of its parameters need to be set by experience in DA,which might not be appropriate for all optimization problems. A self-adapting and efficient dandelion algorithm is proposed in this work to lower the number of DA's parameters and simplify DA's structure. Only the normal sowing operator is retained;while the other operators are discarded. An adaptive seeding radius strategy is designed for the core dandelion. The results show that the proposed algorithm achieves better performance on the standard test functions with less time consumption than its competitive peers. In addition, the proposed algorithm is applied to feature selection for credit card fraud detection(CCFD), and the results indicate that it can obtain higher classification and detection performance than the-state-of-the-art methods. 展开更多
关键词 Credit card fraud detection(CCFD) dandelion algorithm(DA) feature selection normal sowing operator
下载PDF
CARD8基因新发P367L失功能突变患者自身炎症性疾病的临床及免疫学特征 被引量:1
4
作者 甘瑞 周丽娜 +4 位作者 吴俊峰 安云飞 唐雪梅 赵晓东 杨曦 《免疫学杂志》 CAS CSCD 北大核心 2023年第9期821-828,共8页
目的报道1例CARD8基因新发突变导致自身炎症性疾病患儿及其家系的临床特征、致病性鉴定与免疫致病机制。方法收集并分析1例CARD8基因突变患儿的突变位点以及临床资料,随访患儿治疗及预后;采用全外显子测序发现可疑致病基因并利用Sanger... 目的报道1例CARD8基因新发突变导致自身炎症性疾病患儿及其家系的临床特征、致病性鉴定与免疫致病机制。方法收集并分析1例CARD8基因突变患儿的突变位点以及临床资料,随访患儿治疗及预后;采用全外显子测序发现可疑致病基因并利用Sanger测序验证家系突变位点;利用体外实验进行致病性验证及免疫学分析。结果先证者为1名男性患儿,主要临床表现为极早发的食物蛋白过敏性肠炎、特应性皮炎及反复发作的上消化道出血。实验室检查提示血清IgE升高。全外显子测序发现CARD8基因新发杂合突变c.1100C>T(p.P367L),且该突变位点经Sanger测序验证证实来源于其父亲。该突变致病性不明确,且既往未见报道。实验室检查提示患儿血浆中多种促炎细胞因子水平显著升高。患儿原代外周血单个核细胞(PBMCs)及转染相同突变质粒的293T细胞CARD8蛋白表达均显著降低。患儿PBMCs中NLRP3炎性小体过度激活,而NF-κB通路无异常。以上研究均提示患儿的CARD8基因p.P367L突变为失功能性致病突变。结论本文报道全球第2例,中国第1例CARD8基因杂合突变患儿及家系,并明确CRAD8基因c.1100C>T的突变致病性。该CARD8基因失功能突变可导致NLRP3炎性小体的过度激活而诱发患儿发生以消化道症状起病的自身炎症性疾病。 展开更多
关键词 card8 自身炎症性疾病 NLRP3炎性小体
下载PDF
携带罕见突变的CARD14相关丘疹鳞屑性疾病一例
5
作者 陈文杰 周盛基 +3 位作者 陈声利 王真真 刘红 张福仁 《中国麻风皮肤病杂志》 2023年第8期580-582,共3页
患者,男,41岁。因“全身红斑鳞屑40天”来我院就诊,临床、组织病理学具有毛发红糠疹和银屑病的交叉表现,无家族史,首次发病年龄为41岁。初步诊断为I型毛发红糠疹,口服阿维A有显著改善。经过全外显子测序和Sanger测序发现患者携带CARD14... 患者,男,41岁。因“全身红斑鳞屑40天”来我院就诊,临床、组织病理学具有毛发红糠疹和银屑病的交叉表现,无家族史,首次发病年龄为41岁。初步诊断为I型毛发红糠疹,口服阿维A有显著改善。经过全外显子测序和Sanger测序发现患者携带CARD14基因的罕见致病性突变位点c.1799 C>T(p.Pro600Leu),综合临床、组织病理学表现,以及基因检测的结果,最终诊断为CARD14相关的丘疹鳞屑性疾病。 展开更多
关键词 card14 CAPE 毛发红糠疹 银屑病
下载PDF
A Credit Card Fraud Model Prediction Method Based on Penalty Factor Optimization AWTadaboost 被引量:1
6
作者 Wang Ning Siliang Chen +2 位作者 Fu Qiang Haitao Tang Shen Jie 《Computers, Materials & Continua》 SCIE EI 2023年第3期5951-5965,共15页
With the popularity of online payment, how to perform creditcard fraud detection more accurately has also become a hot issue. And withthe emergence of the adaptive boosting algorithm (Adaboost), credit cardfraud detec... With the popularity of online payment, how to perform creditcard fraud detection more accurately has also become a hot issue. And withthe emergence of the adaptive boosting algorithm (Adaboost), credit cardfraud detection has started to use this method in large numbers, but thetraditional Adaboost is prone to overfitting in the presence of noisy samples.Therefore, in order to alleviate this phenomenon, this paper proposes a newidea: using the number of consecutive sample misclassifications to determinethe noisy samples, while constructing a penalty factor to reconstruct thesample weight assignment. Firstly, the theoretical analysis shows that thetraditional Adaboost method is overfitting in a noisy training set, which leadsto the degradation of classification accuracy. To this end, the penalty factorconstructed by the number of consecutive misclassifications of samples isused to reconstruct the sample weight assignment to prevent the classifierfrom over-focusing on noisy samples, and its reasonableness is demonstrated.Then, by comparing the penalty strength of the three different penalty factorsproposed in this paper, a more reasonable penalty factor is selected.Meanwhile, in order to make the constructed model more in line with theactual requirements on training time consumption, the Adaboost algorithmwith adaptive weight trimming (AWTAdaboost) is used in this paper, so thepenalty factor-based AWTAdaboost (PF_AWTAdaboost) is finally obtained.Finally, PF_AWTAdaboost is experimentally validated against other traditionalmachine learning algorithms on credit card fraud datasets and otherdatasets. The results show that the PF_AWTAdaboost method has betterperformance, including detection accuracy, model recall and robustness, thanother methods on the credit card fraud dataset. And the PF_AWTAdaboostmethod also shows excellent generalization performance on other datasets.From the experimental results, it is shown that the PF_AWTAdaboost algorithmhas better classification performance. 展开更多
关键词 Credit card fraud noisy samples penalty factors AWTadaboost algorithm
下载PDF
A Credit Card Fraud Detection Model Based on Multi-Feature Fusion and Generative Adversarial Network 被引量:1
7
作者 Yalong Xie Aiping Li +2 位作者 Biyin Hu Liqun Gao Hongkui Tu 《Computers, Materials & Continua》 SCIE EI 2023年第9期2707-2726,共20页
Credit Card Fraud Detection(CCFD)is an essential technology for banking institutions to control fraud risks and safeguard their reputation.Class imbalance and insufficient representation of feature data relating to cr... Credit Card Fraud Detection(CCFD)is an essential technology for banking institutions to control fraud risks and safeguard their reputation.Class imbalance and insufficient representation of feature data relating to credit card transactions are two prevalent issues in the current study field of CCFD,which significantly impact classification models’performance.To address these issues,this research proposes a novel CCFD model based on Multifeature Fusion and Generative Adversarial Networks(MFGAN).The MFGAN model consists of two modules:a multi-feature fusion module for integrating static and dynamic behavior data of cardholders into a unified highdimensional feature space,and a balance module based on the generative adversarial network to decrease the class imbalance ratio.The effectiveness of theMFGAN model is validated on two actual credit card datasets.The impacts of different class balance ratios on the performance of the four resamplingmodels are analyzed,and the contribution of the two different modules to the performance of the MFGAN model is investigated via ablation experiments.Experimental results demonstrate that the proposed model does better than state-of-the-art models in terms of recall,F1,and Area Under the Curve(AUC)metrics,which means that the MFGAN model can help banks find more fraudulent transactions and reduce fraud losses. 展开更多
关键词 Credit card fraud detection imbalanced classification feature fusion generative adversarial networks anti-fraud systems
下载PDF
柴葛芩连汤对解脲脲原体肺炎小鼠肺功能及CARDS TX/NLRP3信号通路的影响
8
作者 黄方 许诣 +4 位作者 杨雪 汪枝繁 王静 沈文婷 陈霞 《中成药》 CAS CSCD 北大核心 2023年第8期2537-2542,共6页
目的探究柴葛芩连汤对解脲脲原体肺炎模型小鼠肺功能以及CARDS TX/NLRP3信号通路的影响。方法60只小鼠分为对照组,肺炎组,柴葛芩连汤低、高剂量组和阿奇霉素组。鼻腔滴注解脲脲原体菌液进行造模,柴葛芩连汤低、高剂量组灌胃5.4、16.2 g... 目的探究柴葛芩连汤对解脲脲原体肺炎模型小鼠肺功能以及CARDS TX/NLRP3信号通路的影响。方法60只小鼠分为对照组,肺炎组,柴葛芩连汤低、高剂量组和阿奇霉素组。鼻腔滴注解脲脲原体菌液进行造模,柴葛芩连汤低、高剂量组灌胃5.4、16.2 g/kg柴葛芩连汤;阿奇霉素组灌胃90 mg/kg阿奇霉素溶液2个周期(1周期为7 d,前3 d连续灌胃阿奇霉素溶液,后4 d灌胃等量蒸馏水);对照组和肺炎组灌胃等量蒸馏水,连续14 d。采用动物肺功能分析仪检测肺功能,ELISA法检测肺泡灌洗液中炎症因子水平,HE染色观察肺组织病理变化,RT-qPCR和Western blot法检测肺组织肺炎支原体外毒素(CARDS TX)、炎症小体(NLRP3)、凋亡相关斑点样蛋白(ASC)、半胱氨酸天冬氨酸蛋白酶-1(caspase-1)的mRNA和蛋白表达。结果与对照组比较,肺炎组小鼠PEF、FEV0.1、FEF-50降低(P<0.05),IL-6、TNF-α水平及CARDS TX、NLRP3、ASC、caspase-1 mRNA和蛋白表达均升高(P<0.05),肺泡结构模糊,有炎性细胞浸润;与肺炎组比较,柴葛芩连汤各剂量组和阿奇霉素组小鼠PEF、FEV0.1与FEF-50升高(P<0.05),IL-6、TNF-α水平及CARDS TX、NLRP3、ASC、caspase-1 mRNA和蛋白表达均降低(P<0.05),肺组织病理变化有不同程度缓解。结论柴葛芩连汤可以改善解脲脲原体肺炎小鼠受损的肺功能,该作用可能与抑制CARDS TX/NLRP3信号通路有关。 展开更多
关键词 柴葛芩连汤 肺炎 解脲脲原体 cardS TX NLRP3
下载PDF
Effect of a low-cost instruction card for automated external defibrillator operation in lay rescuers: a randomized simulation study
9
作者 Qiang Zhou Xuejie Dong +5 位作者 Wei Zhang Rengyu Wu Kaizhu Chen Hongjuan Zhang Zhijie Zheng Lin Zhang 《World Journal of Emergency Medicine》 SCIE CAS CSCD 2023年第4期265-272,共8页
BACKGROUND:To evaluate whether a simplified self-instruction card can help potential rescue providers use automated external defibrillators(AEDs)more accurately and quickly.METHODS:From June 1,2018,to November 30,2019... BACKGROUND:To evaluate whether a simplified self-instruction card can help potential rescue providers use automated external defibrillators(AEDs)more accurately and quickly.METHODS:From June 1,2018,to November 30,2019,a prospective longitudinal randomized controlled simulation study was conducted among 165 laypeople(18–65 years old)without prior AED training.A self-instruction card was designed to illuminate key AED operation procedures.Subjects were randomly divided into the card(n=83)and control(n=82)groups with age stratification.They were then individually evaluated in the same simulated scenario to use AED with(card group)or without the self-instruction card(control group)at baseline,posttraining,and at the 3-month follow-up.RESULTS:At baseline,the card group reached a significantly higher proportion of successful defibrillation(31.1%vs.15.9%,P=0.03),fully baring the chest(88.9%vs.63.4%,P<0.001),correct electrode placement(32.5%vs.17.1%,P=0.03),and resuming cardiopulmonary resuscitation(CPR)(72.3%vs.9.8%,P<0.001).At post-training and follow-up,there were no significant differences in key behaviors,except for resuming CPR.Time to shock and time to resume CPR were shorter in the card group,while time to power-on AED was not different in each phase of tests.In the 55–65 years group,the card group achieved more skill improvements over the control group compared to the other age groups.CONCLUSION:The self-instruction card could serve as a direction for first-time AED users and as a reminder for trained subjects.This could be a practical,cost-effective way to improve the AED skills of potential rescue providers among different age groups,including seniors. 展开更多
关键词 Automated external defibrillator Laypeople SKILL Instruction card Simulation
下载PDF
Study on Licker-In and Flat Speeds of Carding Machine and Its Effects on Quality of Cotton Spinning Process
10
作者 Md. Mominul Motin Ayub Nabi Khan Md. Obaidur Rahman 《Journal of Textile Science and Technology》 2023年第3期198-214,共17页
Spinning has a significant influence on all textile processes. Combinations of all the capital equipment display the process’ critical condition. By transforming unprocessed fibers into carded sliver and yarn, the ca... Spinning has a significant influence on all textile processes. Combinations of all the capital equipment display the process’ critical condition. By transforming unprocessed fibers into carded sliver and yarn, the carding machine serves a critical role in the textile industry. The carding machine’s licker-in and flat speeds are crucial operational factors that have a big influence on the finished goods’ quality. The purpose of this study is to examine the link between licker-in and flat speeds and how they affect the yarn and carded sliver quality. A thorough experimental examination on a carding machine was carried out to accomplish this. The carded sliver and yarn produced after experimenting with different licker-in and flat speed combinations were assessed for important quality factors including evenness, strength, and flaws. To account for changes in material qualities and machine settings, the study also took into consideration the impact of various fiber kinds and processing circumstances. The findings of the investigation showed a direct relationship between the quality of the carded sliver and yarn and the licker-in and flat speeds. Within a limited range, greater licker-in speeds were shown to increase carding efficiency and decrease fiber tangling. On the other hand, extremely high speeds led to more fiber breakage and neps. Higher flat speeds, on the other hand, helped to enhance fiber alignment, which increased the evenness and strength of the carded sliver and yarn. Additionally, it was discovered that the ideal blend of licker-in and flat rates varied based on the fiber type and processing circumstances. When being carded, various fibers displayed distinctive behaviors that necessitated adjusting the operating settings in order to provide the necessary quality results. The study also determined the crucial speed ratios between the licker-in and flat speeds that reduced fiber breakage and increased the caliber of the finished goods. The results of this study offer useful information for textile producers and process engineers to improve the quality of carded sliver and yarn while maximizing the performance of carding machines. Operators may choose machine settings and parameter adjustments wisely by knowing the impacts of licker-in and flat speeds, which will increase textile industry efficiency, productivity, and product quality. 展开更多
关键词 Spinning Process carding Machine Yarn Count FLAT Licker-In Sliver Hank
下载PDF
Study on Licker-In and Flat Speeds of Carding Machine and Its Effects on Quality of Cotton Spinning Process
11
作者 Md. Mominul Motin Ayub Nabi Khan Md. Obaidur Rahman 《Journal of Flow Control, Measurement & Visualization》 2023年第3期198-214,共17页
Spinning has a significant influence on all textile processes. Combinations of all the capital equipment display the process’ critical condition. By transforming unprocessed fibers into carded sliver and yarn, the ca... Spinning has a significant influence on all textile processes. Combinations of all the capital equipment display the process’ critical condition. By transforming unprocessed fibers into carded sliver and yarn, the carding machine serves a critical role in the textile industry. The carding machine’s licker-in and flat speeds are crucial operational factors that have a big influence on the finished goods’ quality. The purpose of this study is to examine the link between licker-in and flat speeds and how they affect the yarn and carded sliver quality. A thorough experimental examination on a carding machine was carried out to accomplish this. The carded sliver and yarn produced after experimenting with different licker-in and flat speed combinations were assessed for important quality factors including evenness, strength, and flaws. To account for changes in material qualities and machine settings, the study also took into consideration the impact of various fiber kinds and processing circumstances. The findings of the investigation showed a direct relationship between the quality of the carded sliver and yarn and the licker-in and flat speeds. Within a limited range, greater licker-in speeds were shown to increase carding efficiency and decrease fiber tangling. On the other hand, extremely high speeds led to more fiber breakage and neps. Higher flat speeds, on the other hand, helped to enhance fiber alignment, which increased the evenness and strength of the carded sliver and yarn. Additionally, it was discovered that the ideal blend of licker-in and flat rates varied based on the fiber type and processing circumstances. When being carded, various fibers displayed distinctive behaviors that necessitated adjusting the operating settings in order to provide the necessary quality results. The study also determined the crucial speed ratios between the licker-in and flat speeds that reduced fiber breakage and increased the caliber of the finished goods. The results of this study offer useful information for textile producers and process engineers to improve the quality of carded sliver and yarn while maximizing the performance of carding machines. Operators may choose machine settings and parameter adjustments wisely by knowing the impacts of licker-in and flat speeds, which will increase textile industry efficiency, productivity, and product quality. 展开更多
关键词 Spinning Process carding Machine Yarn Count FLAT Licker-In Sliver Hank
下载PDF
Credit Card Fraud Detection on Original European Credit Card Holder Dataset Using Ensemble Machine Learning Technique
12
作者 Yih Bing Chu Zhi Min Lim +3 位作者 Bryan Keane Ping Hao Kong Ahmed Rafat Elkilany Osama Hisham Abusetta 《Journal of Cyber Security》 2023年第1期33-46,共14页
The proliferation of digital payment methods facilitated by various online platforms and applications has led to a surge in financial fraud,particularly in credit card transactions.Advanced technologies such as machin... The proliferation of digital payment methods facilitated by various online platforms and applications has led to a surge in financial fraud,particularly in credit card transactions.Advanced technologies such as machine learning have been widely employed to enhance the early detection and prevention of losses arising frompotentially fraudulent activities.However,a prevalent approach in existing literature involves the use of extensive data sampling and feature selection algorithms as a precursor to subsequent investigations.While sampling techniques can significantly reduce computational time,the resulting dataset relies on generated data and the accuracy of the pre-processing machine learning models employed.Such datasets often lack true representativeness of realworld data,potentially introducing secondary issues that affect the precision of the results.For instance,undersampling may result in the loss of critical information,while over-sampling can lead to overfitting machine learning models.In this paper,we proposed a classification study of credit card fraud using fundamental machine learning models without the application of any sampling techniques on all the features present in the original dataset.The results indicate that Support Vector Machine(SVM)consistently achieves classification performance exceeding 90%across various evaluation metrics.This discovery serves as a valuable reference for future research,encouraging comparative studies on original dataset without the reliance on sampling techniques.Furthermore,we explore hybrid machine learning techniques,such as ensemble learning constructed based on SVM,K-Nearest Neighbor(KNN)and decision tree,highlighting their potential advancements in the field.The study demonstrates that the proposed machine learning models yield promising results,suggesting that pre-processing the dataset with sampling algorithm or additional machine learning technique may not always be necessary.This research contributes to the field of credit card fraud detection by emphasizing the potential of employing machine learning models directly on original datasets,thereby simplifying the workflow and potentially improving the accuracy and efficiency of fraud detection systems. 展开更多
关键词 Machine learning credit card fraud ensemble learning non-sampled dataset hybrid AI models European credit card holder
下载PDF
CARD9在真菌感染性疾病中的作用研究进展
13
作者 杨卓 杨欢 《中国真菌学杂志》 CSCD 2023年第5期434-438,共5页
CARD9是衔接先天性免疫和获得性免疫之间的重要纽带,近年来越来越多的疾病显示出与CARD9的缺失或突变的联系,特别是真菌感染性疾病。该文主要从CARD9在真菌免疫方面的作用机制出发,对与CARD9相关的真菌感染性疾病进行概述,探讨CARD9在... CARD9是衔接先天性免疫和获得性免疫之间的重要纽带,近年来越来越多的疾病显示出与CARD9的缺失或突变的联系,特别是真菌感染性疾病。该文主要从CARD9在真菌免疫方面的作用机制出发,对与CARD9相关的真菌感染性疾病进行概述,探讨CARD9在真菌感染性疾病中的作用。 展开更多
关键词 card9 真菌 侵袭性真菌感染 炎症性肠病 研究进展
下载PDF
NOD样受体家族含CARD结构域5在胃癌中的相互作用蛋白及其对胃癌的诊断价值
14
作者 吴杨 张小敏 郑少华 《癌症进展》 2023年第14期1533-1538,共6页
目的 探究NOD样受体家族含CARD结构域5(NLRC5)在胃癌中的相互作用蛋白,并明确其对胃癌的诊断价值。方法 采用癌症基因组图谱(TCGA)数据库探究NLRC5在胃癌组织和癌旁组织中的差异,结合STRING网站分析NLRC5的相互作用蛋白,通过基因表达综... 目的 探究NOD样受体家族含CARD结构域5(NLRC5)在胃癌中的相互作用蛋白,并明确其对胃癌的诊断价值。方法 采用癌症基因组图谱(TCGA)数据库探究NLRC5在胃癌组织和癌旁组织中的差异,结合STRING网站分析NLRC5的相互作用蛋白,通过基因表达综合(GEO)数据库验证TCGA数据库筛选出来的蛋白对胃癌的诊断价值。引用临床模型,验证筛选出来的蛋白在胃癌组织和癌旁组织中的表达情况,确定各蛋白间的相关性,通过受试者工作特征(ROC)曲线明确其对胃癌的诊断价值。结果 TCGA结果显示,胃癌组织中NLRC5表达水平明显高于癌旁组织(P﹤0.01)。蛋白-蛋白相互作用发现,核因子κB激酶复合物抑制剂组分(CHUK)、核因子κB激酶亚单位β的抑制剂(IKBKB)、三重基序蛋白25(TRIM25)与NLRC5均呈正相关。经过GEO数据库验证得到TRIM25、CHUK、IKBKB与NLRC5可以构建联合模型诊断胃癌。临床模型中NLRC5在胃癌组织中高表达,且与TRIM25、IKBKB、CHUK表达水平均呈正相关(P﹤0.01)。NLRC5联合TRIM25、IKBKB、CHUK对胃癌有较高的诊断价值。结论 TRIM25、IKBKB、CHUK参与NLRC5在胃癌发展中的相关调控机制,在胃癌诊断中具有较高的效能。 展开更多
关键词 癌症基因组图谱 基因表达综合数据库 NOD样受体家族含card结构域5 胃癌 相互作用蛋白 诊断价值
下载PDF
溃疡性结肠炎与NLRP3/CARD8基因多态性的相关性分析
15
作者 杨青华 郭茂东 《健康研究》 CAS 2023年第4期441-444,共4页
目的探讨Nod样受体蛋白3(NLRP3)/炎症小体组分基因胱天蛋白酶募集域蛋白8(CARD8)基因多态性对溃疡性结肠炎(ulcerative colitis,UC)患者易感性的影响。方法分别从96例溃疡性结肠炎患者(UC组)和96例非溃疡结肠炎患者(对照组)的外周血液... 目的探讨Nod样受体蛋白3(NLRP3)/炎症小体组分基因胱天蛋白酶募集域蛋白8(CARD8)基因多态性对溃疡性结肠炎(ulcerative colitis,UC)患者易感性的影响。方法分别从96例溃疡性结肠炎患者(UC组)和96例非溃疡结肠炎患者(对照组)的外周血液样本中提取DNA,采用聚合酶链式反应(PCR)进行扩增,探针法荧光定量PCR检测NLRP3和CARD8不同点位的基因型,应用ABI7900HT Fast Real-Time PCR System自带软件对读取基因分型。UC组患者均接受硫唑嘌呤(AZA)治疗,分析NLRP3和CARD8基因多态性与治疗后结肠炎性反应的相关性。结果NLRP3基因rs10754558位点包含CC、CG、GG 3种基因型,UC组的CC基因型和C等位基因频率(80.20%、88.02%)低于对照组(95.33%、97.92%),CG、GG及G等位基因型频率(15.63%、4.17%、11.98%)高于对照组(4.17%、0、2.08%);CARD8基因rs2443211位点包含AA、AT、TT 3种基因型,UC组AA基因型和A等位基因频率(54.17%、69.79%)较对照组(93.75%、95.83%)降低,AT、TT及T等位基因频率(31.25%、14.58%、30.21%)较对照组(4.17%、2.08%、4.17%)升高;差异均有统计学意义(P<0.05)。UC组患者接受AZA治疗后,缓解率为77.08%;NLPR3 CC基因型、CARD8 AA基因型患者的肠壁炎性反应缓解率分别高于其他基因型者,差异均有统计学意义(P<0.05)。结论NLRP3 CC基因、CARD8 AA基因在肠壁炎性反应的缓解率中发挥作用,NLRP3/CARD8基因多态性均对溃疡性结肠炎患者易感性有一定的影响。 展开更多
关键词 溃疡性结肠炎 NLRP3 card8 基因多态性 相关性分析
下载PDF
基于多交叉置换扩增和纳米生物传感技术快速检测肺炎支原体方法的建立
16
作者 肖飞 郑宝英 +8 位作者 徐文健 伏瑾 黄小兰 孙春荣 贾楠 张裕 许峥 周娟 王毅 《遵义医科大学学报》 2024年第5期513-521,共9页
目的建立一种简单、灵敏、快速的肺炎支原体(MP)检测方法,并对其应用性进行验证和评价。方法利用多交叉置换扩增(MCDA)技术对肺炎支原体特异基因CARDS毒素基因进行扩增,利用侧流免疫层析生物传感(LFB)技术读取扩增结果,命名该方法为MP-M... 目的建立一种简单、灵敏、快速的肺炎支原体(MP)检测方法,并对其应用性进行验证和评价。方法利用多交叉置换扩增(MCDA)技术对肺炎支原体特异基因CARDS毒素基因进行扩增,利用侧流免疫层析生物传感(LFB)技术读取扩增结果,命名该方法为MP-MCDA-LFB。分析扩增反应在60~67℃(间隔1℃)的扩增效率,筛选最适反应温度;分析分别扩增10、20、30、40 min时能够检测到的最低核酸浓度,筛选最佳反应时间。利用10倍系列稀释的肺炎支原体核酸分析MP-MCDA-LFB方法的灵敏度和检测限,利用35株非肺炎支原体菌株分析MP-MCDA-LFB方法的特异性。利用MP-MCDA-LFB方法检测80份疑似MP感染的临床样本,并与RT-PCR法检测结果进行比较,分析MP-MCDA-LFB方法的临床应用性。结果MP-MCDA-LFB能够实现对肺炎支原体CARDS毒素基因的快速检测。其最佳反应温度为63℃,最短反应时间为40 min,整个检测过程可在1 h内。MP-MCDA-LFB方法具有较高的灵敏度和特异性,其检测限低至45 ng/L,与其他临床表现相似的病原体无交叉反应,特异性为100%。MP-MCDA-LFB方法从80份临床样本中检出45份阳性样本(56.3%),检出率与RT-PCR方法一致。结论本研究建立的以CARDS毒素基因为靶标的MP-MCDA-LFB检测方法具有简单、快速、灵敏度高、特异性强的优点,在基层医疗机构和现场检测具有较好的应用潜力。 展开更多
关键词 肺炎支原体 多交叉置换扩增技术 侧流免疫层析生物传感技术 cardS毒素基因 RT-PCR
下载PDF
平衡计分卡在公共部门预算绩效管理中的应用分析——基于学习与成长视角的考察 被引量:1
17
作者 马蔡琛 林江 《云南财经大学学报》 北大核心 2024年第1期32-40,共9页
作为平衡计分卡的基础,学习与成长视角不仅是平衡计分卡价值创造的动力和源泉,而且融入了预算绩效管理的各个环节。但是在具体应用中,平衡计分卡仍然面临着学习反馈与人才培养机制有待完善、预算绩效信息质量急需提高等一系列挑战。在... 作为平衡计分卡的基础,学习与成长视角不仅是平衡计分卡价值创造的动力和源泉,而且融入了预算绩效管理的各个环节。但是在具体应用中,平衡计分卡仍然面临着学习反馈与人才培养机制有待完善、预算绩效信息质量急需提高等一系列挑战。在未来的改革中,应不断优化预算绩效管理中的学习反馈机制,将奖惩激励措施有机融入人才培养机制,通过多元化数据来源和建立平衡计分卡专项数据库等手段,持续提升预算绩效信息管理的技术水平。 展开更多
关键词 预算绩效管理 平衡计分卡 学习与成长视角
下载PDF
数字化转型背景下校园卡数据治理研究与实践
18
作者 展鹏 乔禹 李腾 《中国教育信息化》 2024年第4期51-60,共10页
教育数字化转型是贯彻落实科教兴国、人才强国、创新驱动发展的重要先手棋,数据是高校推进数字化转型的关键要素与重要战略资源,如何充分且有效地利用数据资源推进业务模式创新,营造数字化、智能化、个性化的教育教学环境已成为高校数... 教育数字化转型是贯彻落实科教兴国、人才强国、创新驱动发展的重要先手棋,数据是高校推进数字化转型的关键要素与重要战略资源,如何充分且有效地利用数据资源推进业务模式创新,营造数字化、智能化、个性化的教育教学环境已成为高校数字化转型建设上的一项必答题。作为高校信息化建设的基础工程,校园卡系统处在数据治理与业务融合创新的最前沿,并发挥重要作用。为提升数据治理效能,坚持问题导向,梳理数据治理整体思路,设计实现包含数据标准体系、基础支撑体系和组织保障体系的校园卡数据治理体系框架。在此框架基础上,以“应用为王、服务至上、简洁高效、安全运行”的工作要求和原则为指导,以创新应用与服务需求为驱动,加强统筹谋划,做好校园卡数据治理顶层设计,将数据作为业务化基础支撑,为推进校园卡业务智能化进程提供标准化数据服务,助推基于校园卡系统的高校校园生活服务大平台的数字化转型。 展开更多
关键词 教育数字化 校园卡 数据治理 架构体系
下载PDF
建行信用卡系统全栈国产化改造研究
19
作者 金磐石 张晓东 +4 位作者 邢磊 李晓栋 彭云 杨永 李铮 《计算机技术与发展》 2024年第6期192-200,共9页
信用卡业务是业务逻辑最复杂的银行业务之一,其对可用性、可靠性、处理性能要求较高。从技术发展角度来看,多技术栈融合的新型IT架构,符合云计算资源池化的趋势。从业务角度来看,为满足不同的业务需求,同样存在多技术栈融合架构的诉求... 信用卡业务是业务逻辑最复杂的银行业务之一,其对可用性、可靠性、处理性能要求较高。从技术发展角度来看,多技术栈融合的新型IT架构,符合云计算资源池化的趋势。从业务角度来看,为满足不同的业务需求,同样存在多技术栈融合架构的诉求。然而,多计算架构并非简单实现一个全新的技术栈即可,需要解决架构改造与设计、系统验证、兼容性以及故障切换等一系列问题。面对上述挑战,该文面向金融IT系统高并发、高性能以及高可用需求,介绍了建行面向金融行业的高性能、高可用和高安全可靠的x86、ARM双平台混合架构系统中的设计与思考。通过一系列的代码迁移、应用迁移以及系统垂直优化技术,实现高性能、高可用和高安全的诉求,并在建行得到了大规模、长时间的真实系统验证。 展开更多
关键词 ARM 多计算架构 服务器 信用卡系统 代码迁移
下载PDF
卡片导航平衡控制系统性能仿真研究
20
作者 刘建军 苏意芬 +1 位作者 廖志华 陈庆新 《工业工程》 2024年第1期54-64,共11页
为了减少装配作业车间内因物料齐套产生的等待浪费,使具有装配约束的关联零件加工进度得到有效协同,设计一类卡片导航平衡控制系统(control of balance by card-based navigation,COBACABANA)。其基于两类卡片循环回路实现任务投放与作... 为了减少装配作业车间内因物料齐套产生的等待浪费,使具有装配约束的关联零件加工进度得到有效协同,设计一类卡片导航平衡控制系统(control of balance by card-based navigation,COBACABANA)。其基于两类卡片循环回路实现任务投放与作业分派的可视化进度协同控制逻辑。本文详细介绍系统的运行机制和系统控制参量,通过构建一般化的装配作业车间仿真模型,探讨在不同装配关联度下各控制参量的性能变化。实验结果表明,COBACABANA系统性能良好,并且选择合适的控制参量就能够有效提升关联零件的进度协同性。 展开更多
关键词 装配作业车间 精益生产控制 任务投放 作业分派 卡片系统
下载PDF
上一页 1 2 250 下一页 到第
使用帮助 返回顶部