We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/...We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.3.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.3.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.展开更多
Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recom...Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.3.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.3.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.3.4.4 b HA gene, an H5 N6 virus bearing the clade 2.3.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.3.4.4 h or 2.3.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature.展开更多
Fine grained Mg-7Gd-5Y-1.2Nd-0.5Zr alloy was investigated by dynamic compression tests using a Split Hopkinson Pressure Bar under the strain rates in the range 1000-2000 s^(-1) and the temperature range 293-573 K alon...Fine grained Mg-7Gd-5Y-1.2Nd-0.5Zr alloy was investigated by dynamic compression tests using a Split Hopkinson Pressure Bar under the strain rates in the range 1000-2000 s^(-1) and the temperature range 293-573 K along the normal direction.The microstructure was measured by optical microscopy,electron back-scattering diffraction,transmission electron microscopy and X-ray diffractometry.The results showed that Mg-7Gd-5Y-1.2Nd-0.5Zr alloy had the positive strain rate strengthening effect and thermal softening effect at high temperature.The solid solution of Gd and Y atoms in Mg-7Gd-5Y-1.2Nd-0.5Zr alloy reduced the asymmetry of α-Mg crystals and changed the critical shear stress of various deformation mechanisms.The main deformation mechanisms were prismatic slip and pyramidal(a)slip,{102}tension twinning,and dynamic recrystallization caused by local deformation such as particle-stimulated nucleation.c 2020 Published by Elsevier B.V.on behalf of Chongqing University.展开更多
基金This work was supported by the National Key R&D Program of China(2016YFD0501602,2017YFD0500701,and 2016YFEO203200)the National Natural Science Foundation of China(3167131307)+1 种基金the China Agriculture Research System(CARS-41-G12)and Central Publicinterest Scientific Institution Basal Research Fund(1610302017001).
文摘We developed an H5/H7 trivalent inactivated vaccine by using Re-11, Re-12, and H7-Re2 vaccine seed viruses, which were generated by reverse genetics and derived their HA genes from A/duck/Guizhou/S4184/2017(H5N6) (DK/GZ/S4184/17) (a clade 2.3.4.4d virus), A/chicken/Liaoning/SD007/2017(H5N1) (CK/LN/SD007/17) (a clade 2.3.2.1d virus), and A/chicken/ Guangxi/SD098/2017(H7N9) (CK/GX/SD098/17), respectively. The protective efficacy of this novel vaccine and that of the recently used H5/H7 bivalent inactivated vaccine against different H5 and H7N9 viruses was evaluated in chickens. We found that the H5/H7 bivalent vaccine provided solid protection against the H7N9 virus CK/GX/SD098/17, but only 50–60% protection against different H5 viruses. In contrast, the novel H5/H7 trivalent vaccine provided complete protection against the H5 and H7 viruses tested. Our study underscores the importance of timely updating of vaccines for avian influenza control.
基金supported by the National Key Research and Development Program of China(2021YFD1800200)the Laboratory for Lingnan Modern Agriculture Project(NT2021007)the China Agriculture Research System of the MOF and MARA(CARS-41-G12)。
文摘Some H5 viruses isolated in poultry or wild birds between 2020 and 2021 were found to be antigenically different from the vaccine strains(H5-Re11 and H5-Re12) used in China. In this study, we generated three new recombinant vaccine seed viruses by using reverse genetics and used them for vaccine production. The vaccine strain H5-Re13 contains the hemagglutinin(HA) and neuraminidase(NA) genes of an H5 N6 virus that bears the clade 2.3.4.4 h HA gene, H5-Re14 contains the HA and NA genes of an H5 N8 virus that bears the clade 2.3.4.4 b HA gene, and H7-Re4 contains the HA and NA genes of H7 N9 virus detected in 2021. We evaluated the protective efficacy of the novel H5/H7 trivalent inactivated vaccine in chickens, ducks, and geese. The inactivated vaccine was immunogenic and induced substantial antibody responses in the birds tested. Three weeks after vaccination, chickens were challenged with five different viruses detected in 2020 and 2021: three viruses(an H5 N1 virus, an H5 N6 virus, and an H5 N8 virus) bearing the clade 2.3.4.4 b HA gene, an H5 N6 virus bearing the clade 2.3.4.4 h HA gene, and an H7 N9 virus. All of the control birds shed high titers of virus and died within 4 days post-challenge, whereas the vaccinated chickens were completely protected from these viruses. Similar protective efficacy against H5 viruses bearing the clade 2.3.4.4 h or 2.3.4.4 b HA gene was observed in ducks and geese. Our study indicates that the newly updated H5/H7 vaccine can provide solid protection against the H5 and H7 N9 viruses that are currently circulating in nature.
基金National Natural Science Foundation of China(Nos.51571145,51404137)City of Ningbo"science and technology innovation 2025"major special project(new energy vehicle lightweight magnesium alloy material precision forming technology)(No.2018B10045).
文摘Fine grained Mg-7Gd-5Y-1.2Nd-0.5Zr alloy was investigated by dynamic compression tests using a Split Hopkinson Pressure Bar under the strain rates in the range 1000-2000 s^(-1) and the temperature range 293-573 K along the normal direction.The microstructure was measured by optical microscopy,electron back-scattering diffraction,transmission electron microscopy and X-ray diffractometry.The results showed that Mg-7Gd-5Y-1.2Nd-0.5Zr alloy had the positive strain rate strengthening effect and thermal softening effect at high temperature.The solid solution of Gd and Y atoms in Mg-7Gd-5Y-1.2Nd-0.5Zr alloy reduced the asymmetry of α-Mg crystals and changed the critical shear stress of various deformation mechanisms.The main deformation mechanisms were prismatic slip and pyramidal(a)slip,{102}tension twinning,and dynamic recrystallization caused by local deformation such as particle-stimulated nucleation.c 2020 Published by Elsevier B.V.on behalf of Chongqing University.
