AIM: To investigate the potential therapeutic effect of exopolysaccharide (EPS)-producing Streptococcus thermophilus (S. thermophilus) CRL 1190 fermented milk on chronic gastritis in Balb/c mice. METHODS: Balb/c mice ...AIM: To investigate the potential therapeutic effect of exopolysaccharide (EPS)-producing Streptococcus thermophilus (S. thermophilus) CRL 1190 fermented milk on chronic gastritis in Balb/c mice. METHODS: Balb/c mice were fed with the fermented milk for 7 d after inducing gastritis with acetyl-salicylic acid (ASA, 400 mg/kg body weight per day for 10 d). Omeprazole was included in this study as a positive therapeutic control. The gastric in? ammatory activity was evaluated from gastric histology and in? ammation score, number of interleukin-10 (IL-10), interferon-γ (INFγ) and tumor necrosis factor-α (TNF-α) cytokine-producing cells in the gastric mucosa, and thickness of the mucus layer. RESULTS: Animals receiving treatment with the EPS-producing S. thermophilus CRL 1190 fermented milk showed a conserved gastric mucosa structure similar to that of healthy animals. In? ammation scores of the fermented milk-treated mice were lower than those of mice in the gastritis group (0.2 ± 0.03 vs 2.0 ± 0.6, P < 0.05). A marked decrease in INFγ+ (15 ± 1.0 vs 28 ± 1.2, P < 0.05) and TNF-α+ (16 ± 3.0 vs 33 ± 3.0, P < 0.05) cells and an increase in IL-10+ (28 ± 1.5 vs 14 ± 1.3, P < 0.05) cells compared to the gastritis group, was observed. Also, an increase in the thickness of the mucus gel layer (2.2 ± 0.6 vs 1.0 ± 0.3; 5.1 ± 0.8 vs 1.5 ± 0.4 in the corpus and antrum mucosa, respectively, P < 0.05) compared with the gastritis group was noted. A milk suspension of the purif ied EPS from S. thermophilus CRL1190 was also effective as therapy for gastritis.展开更多
Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodon...Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodontitis. Gingivitis is a reversible inflammation of the gingiva which can be cured. As with humans, the harmful bacteria can cause infections also in organs such as the heart, liver and kidneys. Besides veterinary treatment, also the owner’s everyday care is essential. Objective: The aim of the study was to develop and clinically test easy home care probiotic product for cats’ and dogs’ oral health. Methods: Nine cats and thirteen dogs were given a combination of selected bacteria Streptococcus thermophilus SP4, Lactobacillus plantarum 14D and Lactobacillus rhamnosus SP1 (Petguide powder) in powder form daily for four weeks on top of the dry feed. Microbiological samples were taken before and after the intervention from each dog and cat. The samples were analyzed with flow cytometric analysis using Porphyromonas gingivalis and Fusobacterium sp. as indicators for oral health. Results: The home care treatment with these specific, live probiotic bacteria was shown to prevent the growth of infectious bacteria within four weeks. Petguide probiotic powder reduced the relative abundance of infectious microbes significantly (p = 0.005) in cats and dogs. Conclusion: The results suggest that the probiotic product with three live strains of probiotic bacteria prevents the growth of infectious bacterial species that are known to cause infections in pets’ mouths. Therefore, this probiotic combination could be used to improve the oral health of pets and increase their quality of life.展开更多
基金Supported by CONICET, ANPCyT (project BID 1728 OC/AR PICTR 20801) and CIUNT, Argentina
文摘AIM: To investigate the potential therapeutic effect of exopolysaccharide (EPS)-producing Streptococcus thermophilus (S. thermophilus) CRL 1190 fermented milk on chronic gastritis in Balb/c mice. METHODS: Balb/c mice were fed with the fermented milk for 7 d after inducing gastritis with acetyl-salicylic acid (ASA, 400 mg/kg body weight per day for 10 d). Omeprazole was included in this study as a positive therapeutic control. The gastric in? ammatory activity was evaluated from gastric histology and in? ammation score, number of interleukin-10 (IL-10), interferon-γ (INFγ) and tumor necrosis factor-α (TNF-α) cytokine-producing cells in the gastric mucosa, and thickness of the mucus layer. RESULTS: Animals receiving treatment with the EPS-producing S. thermophilus CRL 1190 fermented milk showed a conserved gastric mucosa structure similar to that of healthy animals. In? ammation scores of the fermented milk-treated mice were lower than those of mice in the gastritis group (0.2 ± 0.03 vs 2.0 ± 0.6, P < 0.05). A marked decrease in INFγ+ (15 ± 1.0 vs 28 ± 1.2, P < 0.05) and TNF-α+ (16 ± 3.0 vs 33 ± 3.0, P < 0.05) cells and an increase in IL-10+ (28 ± 1.5 vs 14 ± 1.3, P < 0.05) cells compared to the gastritis group, was observed. Also, an increase in the thickness of the mucus gel layer (2.2 ± 0.6 vs 1.0 ± 0.3; 5.1 ± 0.8 vs 1.5 ± 0.4 in the corpus and antrum mucosa, respectively, P < 0.05) compared with the gastritis group was noted. A milk suspension of the purif ied EPS from S. thermophilus CRL1190 was also effective as therapy for gastritis.
文摘Background: Periodontal infections are the most common disease of cats and dogs. About 70% - 80% of them suffer from these infections during their lifetime. These infections occur in two forms, gingivitis and periodontitis. Gingivitis is a reversible inflammation of the gingiva which can be cured. As with humans, the harmful bacteria can cause infections also in organs such as the heart, liver and kidneys. Besides veterinary treatment, also the owner’s everyday care is essential. Objective: The aim of the study was to develop and clinically test easy home care probiotic product for cats’ and dogs’ oral health. Methods: Nine cats and thirteen dogs were given a combination of selected bacteria Streptococcus thermophilus SP4, Lactobacillus plantarum 14D and Lactobacillus rhamnosus SP1 (Petguide powder) in powder form daily for four weeks on top of the dry feed. Microbiological samples were taken before and after the intervention from each dog and cat. The samples were analyzed with flow cytometric analysis using Porphyromonas gingivalis and Fusobacterium sp. as indicators for oral health. Results: The home care treatment with these specific, live probiotic bacteria was shown to prevent the growth of infectious bacteria within four weeks. Petguide probiotic powder reduced the relative abundance of infectious microbes significantly (p = 0.005) in cats and dogs. Conclusion: The results suggest that the probiotic product with three live strains of probiotic bacteria prevents the growth of infectious bacterial species that are known to cause infections in pets’ mouths. Therefore, this probiotic combination could be used to improve the oral health of pets and increase their quality of life.
