Three M_(W)>7.0 earthquakes in 2020-2021 occurred in the Shumagin seismic gap and its adjacent area of the Alaska-Aleutian subduction zone,including the Mw7.8 Simeonof thrust earthquake on July 22,2020,the M_(W)7.6...Three M_(W)>7.0 earthquakes in 2020-2021 occurred in the Shumagin seismic gap and its adjacent area of the Alaska-Aleutian subduction zone,including the Mw7.8 Simeonof thrust earthquake on July 22,2020,the M_(W)7.6 Sand Point strike-slip earthquake on October 19,2020,and the M_(W)8.2 Chignik thrust earthquake on July 29,2021.The spatial and temporal proximity of these three earthquakes prompts us to probe stress-triggering effects among them.Here we examine the coseismic Coulomb stress change imparted by the three earthquakes and their influence on the subduction interface.Our results show that:(1)The Simeonof earthquake has strong loading effects on the subsequent Sand Point and Chignik earthquakes,with the Coulomb stress changes of 3.95 bars and 2.89 bars,respectively.The Coulomb stress change caused by the Sand Point earthquake at the hypocenter of the Chignik earthquake is merely around 0.01 bars,suggesting the negligible triggering effect on the latter earthquake;(2)The triggering effects of the Simeonof,Sand Point,and Chignik earthquakes on aftershocks within three months are not well pronounced because of the triggering rates of 38%,14%,and 43%respectively.Other factors may have played an important role in promoting the occurrence of these aftershocks,such as the roughness of the subduction interface,the complicated velocity structure of the lithosphere,and the heterogeneous prestress therein;(3)The three earthquakes caused remarkable coseismic Coulomb stress changes at the subduction interface nearby these mainshocks,with an average Coulomb stress change of 3.2 bars in the shallow region directly inwards the trench.展开更多
Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeut...Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeutic drugs and targets for diabetes-related sepsis.The research also incorporates traditional physical therapy perspectives,emphasizing the genomic insights gained from exercise therapy in disease management and prevention.Methods:Gene analysis was conducted on the GSE168796 and GSE94717 datasets to identify ER stress-related genes.Gene interactions and immune cell correlations were mapped using GeneCard and STRING databases.A screening of 2,456 compounds from the TCMSP database was performed to identify potential therapeutic agents,with a focus on their docking potential.Techniques such as luciferase reporter gene assay and RNA interference were used to examine the interactions between microRNA-149-5p and MMP9.Results:The study identified 2,006 differentially expressed genes and 616 miRNAs.Key genes like MMP9,TNF-α,and IL1B were linked to an immunosuppressive state.Licorice glycoside E demonstrated high affinity for MMP9,suggesting its potential effectiveness in treating diabetes.The constructed miRNA network highlighted the regulatory roles of MMP9,IL1B,IFNG,and TNF-α.Experimental evidence confirmed the binding of microRNA-149-5p to MMP9,impacting apoptosis in diabetic cells.Conclusion:The findings highlight the regulatory role of microRNA-149-5p in managing MMP9,a crucial gene in diabetes pathophysiology.Licorice glycoside E emerges as a promising treatment option for diabetes,especially targeting MMP9 affected by ER stress.The study also underscores the significance of physical exercise in modulating ER stress pathways in diabetes management,bridging traditional physical therapy and modern scientific understanding.Our study has limitations.It focuses on the microRNA-149-5p-MMP9 network in sepsis,using cell-based methods without animal or clinical trials.Despite strong in vitro findings,in vivo studies are needed to confirm licorice glycoside E’s therapeutic potential and understand the microRNA-149-5p-MMP9 dynamics in real conditions.展开更多
We previously reported that miR-124-3p is markedly upregulated in microglia-derived exosomes following repetitive mild traumatic brain injury.However,its impact on neuronal endoplasmic reticulum stress following repet...We previously reported that miR-124-3p is markedly upregulated in microglia-derived exosomes following repetitive mild traumatic brain injury.However,its impact on neuronal endoplasmic reticulum stress following repetitive mild traumatic brain injury remains unclear.In this study,we first used an HT22 scratch injury model to mimic traumatic brain injury,then co-cultured the HT22 cells with BV2 microglia expressing high levels of miR-124-3p.We found that exosomes containing high levels of miR-124-3p attenuated apoptosis and endoplasmic reticulum stress.Furthermore,luciferase reporter assay analysis confirmed that miR-124-3p bound specifically to the endoplasmic reticulum stress-related protein IRE1α,while an IRE1αfunctional salvage experiment confirmed that miR-124-3p targeted IRE1αand reduced its expression,thereby inhibiting endoplasmic reticulum stress in injured neurons.Finally,we delivered microglia-derived exosomes containing miR-124-3p intranasally to a mouse model of repetitive mild traumatic brain injury and found that endoplasmic reticulum stress and apoptosis levels in hippocampal neurons were significantly reduced.These findings suggest that,after repetitive mild traumatic brain injury,miR-124-3 can be transferred from microglia-derived exosomes to injured neurons,where it exerts a neuroprotective effect by inhibiting endoplasmic reticulum stress.Therefore,microglia-derived exosomes containing miR-124-3p may represent a novel therapeutic strategy for repetitive mild traumatic brain injury.展开更多
In practical engineering,the total vertical stress in the soil layer is not constant due to stress diffusion,and varies with time and depth.Therefore,the purpose of this paper is to investigate the effect of stress di...In practical engineering,the total vertical stress in the soil layer is not constant due to stress diffusion,and varies with time and depth.Therefore,the purpose of this paper is to investigate the effect of stress diffusion on the two-dimensional(2D)plane strain consolidation properties of unsaturated soils when the stress varies with time and depth.A series of semi-analytical solutions in terms of excess pore air and water pressures and settlement for 2D plane strain consolidation of unsaturated soils can be derived with the joint use of Laplace transform and Fourier sine series expansion.Then,the inverse Laplace transform of the semi-analytical solution is given in the time domain using a self-programmed code based on Crump’s method.The reliability of the obtained solutions is proved by the degeneration.Finally,the 2D plots of excess pore pressures and the curves of settlement varying with time,considering different physical parameters of unsaturated soil stratum and depth-dependent stress,are depicted and analyzed to study the 2D plane strain consolidation properties of unsaturated soils subjected to the depthdependent stress.展开更多
Estimation of in situ stresses based on back-analysis of measured stress changes and displacements has become an alternative to the direct stress measurement methods.In order to help users conduct own measurement and ...Estimation of in situ stresses based on back-analysis of measured stress changes and displacements has become an alternative to the direct stress measurement methods.In order to help users conduct own measurement and analysis,this paper presents in detail a field stress back-analysis approach directly from borehole strain changes measured during nearby underground excavation.Essential formulations in major steps and the procedure for the entire analysis process are provided to allow users to follow.The instrument for borehole strain change measurement can be the CSIR or CSIRO stress cells and other borehole strain cells that can measure strains on borehole walls.Strain changes corresponding to the stress changes at a borehole location are calculated in borehole environment.The stress changes due to nearby excavation can be calculated by an analytical model for a single circular opening and simulated by a numerical model for non-circular and multiple openings.These models are based on isotropic,homogeneous and linear elastic assumptions.The analysis of borehole strain changes is accomplished by multiple linear regression based on error minimization and an integrated process provides the best-fit solution directly to the in situ stresses.A statistical technique is adopted for screening outliers in the measurement data,checking measurement compatibility and evaluating the reliability of analysis results.An application example is included to demonstrate the practical application and the analysis procedure.展开更多
This paper analyzed the characteristics of welding solidification crack of stainless steels,and clearly re- vealed the the of the deformation in the molten - the pool and the solidification shrinkage on the stress -...This paper analyzed the characteristics of welding solidification crack of stainless steels,and clearly re- vealed the the of the deformation in the molten - the pool and the solidification shrinkage on the stress - strain fields in the trail of molten - weld pool.Moreover, rheologic properties of the alloys in solid - liquid zone were also obtained by measuring the hading and unloading deform curves of the steels.As a result, a numerical model for simulation of stress - strain distributions of welding solidifi- cation crack was developed. On the basis of the model,the thesis simulated the driving force of solidifi- cation crack of stainless steels, that is, stress - strain fields in the trail of molten-weld pool with fi- nite element method.展开更多
An analytical model for dynamic recrystallization (DRX) is studied based on the relative grain size model proposed by Sakai and Jonas, and the characteristic flow behaviors under DRX are analyzed and simulated. Int...An analytical model for dynamic recrystallization (DRX) is studied based on the relative grain size model proposed by Sakai and Jonas, and the characteristic flow behaviors under DRX are analyzed and simulated. Introducing the variation of dynamic grain size and the heterogeneous distribution of disolo- cation densities densities under DRX,a simple method for modeling and simulating DRX processes is developed by using Laplace transformation theory. The results derived from the present model agree well with the experimental results in literatures. This simulation can reproduce a number of features in DRX flow behaviors, for example,single and multiple peak flow behaviors followed by a steady state flow, the transition between them, and so on.展开更多
The mechanical behavior of plastic concrete used in the cut-off walls of earth dams has been studied.Triaxial compression tests on the specimens in various ages and mix designs under different confining pressures have...The mechanical behavior of plastic concrete used in the cut-off walls of earth dams has been studied.Triaxial compression tests on the specimens in various ages and mix designs under different confining pressures have been done and the stress-strain behavior of such materials and their strength parameter changes have been experimentally investigated.It has been observed that increasing the confining pressures applied on the specimens causes the material behavior to be alike the more ductile materials and the compressive strength increases considerably as well.Moreover,a parametric study has been carried out to investigate the influence of essential parameters on the shear strength parameters of these materials.According to the research,increasing the coarse to fine aggregates ratio leads to the increase of compressive strength of the specimens as well as the increase of the cohesion and internal friction angle of the materials.Furthermore,the bentonite content decrease and the cement factor increase result in an increase of the cohesion parameter of plastic concretes and decrease of the internal friction angle of such materials.展开更多
A more accurate estimation of stress-strain relationships for martensite and ferrite was developed, and the modified law of mixture was used to investigate the stress-strain partitioning of constituent phases in dual ...A more accurate estimation of stress-strain relationships for martensite and ferrite was developed, and the modified law of mixture was used to investigate the stress-strain partitioning of constituent phases in dual phase （DP） steels with two different martensite volume fractions. The results show that there exist great differences in the stress-strain contribution of martensite and ferrite to DP steel. The stress-strain partitioning coefficient is not constant in the whole strain range, but decreases with increasing the true strain of DP steel. The softening effect caused by the dilution of carbon concentration in martensite with the increase of martensite volume fraction has great influence on the strain contribution of martensite. The strain ratio of ferrite to martensite almost linearly increases with increasing the true strain of DP steel when the martensite volume fraction is 22%, because martensite always keeps elastic. But the strain ratio of ferrite to martensite varies indistinctively with the further increase in true strain of DP steel above 0.034 when the martensite volume fraction is 50%, because plastic deformation happens in martensite. The stress ratio ofmartensite to ferrite decreases monotonously with increasing the true strain of DP steel whether the martensite volume fraction is 22% or 50%.展开更多
基金supported by grants from the National Natural Science Foundation of China(Grant No.sU2139205,41774011,41874011)the National Key Research and Development Program of China(Grant No.2018YFC1503605)。
文摘Three M_(W)>7.0 earthquakes in 2020-2021 occurred in the Shumagin seismic gap and its adjacent area of the Alaska-Aleutian subduction zone,including the Mw7.8 Simeonof thrust earthquake on July 22,2020,the M_(W)7.6 Sand Point strike-slip earthquake on October 19,2020,and the M_(W)8.2 Chignik thrust earthquake on July 29,2021.The spatial and temporal proximity of these three earthquakes prompts us to probe stress-triggering effects among them.Here we examine the coseismic Coulomb stress change imparted by the three earthquakes and their influence on the subduction interface.Our results show that:(1)The Simeonof earthquake has strong loading effects on the subsequent Sand Point and Chignik earthquakes,with the Coulomb stress changes of 3.95 bars and 2.89 bars,respectively.The Coulomb stress change caused by the Sand Point earthquake at the hypocenter of the Chignik earthquake is merely around 0.01 bars,suggesting the negligible triggering effect on the latter earthquake;(2)The triggering effects of the Simeonof,Sand Point,and Chignik earthquakes on aftershocks within three months are not well pronounced because of the triggering rates of 38%,14%,and 43%respectively.Other factors may have played an important role in promoting the occurrence of these aftershocks,such as the roughness of the subduction interface,the complicated velocity structure of the lithosphere,and the heterogeneous prestress therein;(3)The three earthquakes caused remarkable coseismic Coulomb stress changes at the subduction interface nearby these mainshocks,with an average Coulomb stress change of 3.2 bars in the shallow region directly inwards the trench.
文摘Background:This study explores the relationship between endoplasmic reticulum(ER)stress and diabetes,particularly focusing on the impact of physical exercise on ER stress mechanisms and identifying potential therapeutic drugs and targets for diabetes-related sepsis.The research also incorporates traditional physical therapy perspectives,emphasizing the genomic insights gained from exercise therapy in disease management and prevention.Methods:Gene analysis was conducted on the GSE168796 and GSE94717 datasets to identify ER stress-related genes.Gene interactions and immune cell correlations were mapped using GeneCard and STRING databases.A screening of 2,456 compounds from the TCMSP database was performed to identify potential therapeutic agents,with a focus on their docking potential.Techniques such as luciferase reporter gene assay and RNA interference were used to examine the interactions between microRNA-149-5p and MMP9.Results:The study identified 2,006 differentially expressed genes and 616 miRNAs.Key genes like MMP9,TNF-α,and IL1B were linked to an immunosuppressive state.Licorice glycoside E demonstrated high affinity for MMP9,suggesting its potential effectiveness in treating diabetes.The constructed miRNA network highlighted the regulatory roles of MMP9,IL1B,IFNG,and TNF-α.Experimental evidence confirmed the binding of microRNA-149-5p to MMP9,impacting apoptosis in diabetic cells.Conclusion:The findings highlight the regulatory role of microRNA-149-5p in managing MMP9,a crucial gene in diabetes pathophysiology.Licorice glycoside E emerges as a promising treatment option for diabetes,especially targeting MMP9 affected by ER stress.The study also underscores the significance of physical exercise in modulating ER stress pathways in diabetes management,bridging traditional physical therapy and modern scientific understanding.Our study has limitations.It focuses on the microRNA-149-5p-MMP9 network in sepsis,using cell-based methods without animal or clinical trials.Despite strong in vitro findings,in vivo studies are needed to confirm licorice glycoside E’s therapeutic potential and understand the microRNA-149-5p-MMP9 dynamics in real conditions.
基金supported by the Haihe Laboratory of Cell Ecosystem Innovation Fund,No.22HHXBSS00047(to PL)the National Natural Science Foundation of China,Nos.82072166(to PL),82071394(to XG)+4 种基金Science and Technology Planning Project of Tianjin,No.20YFZCSY00030(to PL)Science and Technology Project of Tianjin Municipal Health Commission,No.TJWJ2021QN005(to XG)Tianjin Key Medical Discipline(Specialty)Construction Project,No.TJYXZDXK-006ATianjin Municipal Education Commission Scientific Research Program Project,No.2020KJ164(to JZ)China Postdoctoral Science Foundation,No.2022M712392(to ZY).
文摘We previously reported that miR-124-3p is markedly upregulated in microglia-derived exosomes following repetitive mild traumatic brain injury.However,its impact on neuronal endoplasmic reticulum stress following repetitive mild traumatic brain injury remains unclear.In this study,we first used an HT22 scratch injury model to mimic traumatic brain injury,then co-cultured the HT22 cells with BV2 microglia expressing high levels of miR-124-3p.We found that exosomes containing high levels of miR-124-3p attenuated apoptosis and endoplasmic reticulum stress.Furthermore,luciferase reporter assay analysis confirmed that miR-124-3p bound specifically to the endoplasmic reticulum stress-related protein IRE1α,while an IRE1αfunctional salvage experiment confirmed that miR-124-3p targeted IRE1αand reduced its expression,thereby inhibiting endoplasmic reticulum stress in injured neurons.Finally,we delivered microglia-derived exosomes containing miR-124-3p intranasally to a mouse model of repetitive mild traumatic brain injury and found that endoplasmic reticulum stress and apoptosis levels in hippocampal neurons were significantly reduced.These findings suggest that,after repetitive mild traumatic brain injury,miR-124-3 can be transferred from microglia-derived exosomes to injured neurons,where it exerts a neuroprotective effect by inhibiting endoplasmic reticulum stress.Therefore,microglia-derived exosomes containing miR-124-3p may represent a novel therapeutic strategy for repetitive mild traumatic brain injury.
基金supported by the National Natural Science Foundation of China(Grant Nos.12172211 and 41630633)the National Key Research and Development Project of China(Grant No.2019YFC1509800).
文摘In practical engineering,the total vertical stress in the soil layer is not constant due to stress diffusion,and varies with time and depth.Therefore,the purpose of this paper is to investigate the effect of stress diffusion on the two-dimensional(2D)plane strain consolidation properties of unsaturated soils when the stress varies with time and depth.A series of semi-analytical solutions in terms of excess pore air and water pressures and settlement for 2D plane strain consolidation of unsaturated soils can be derived with the joint use of Laplace transform and Fourier sine series expansion.Then,the inverse Laplace transform of the semi-analytical solution is given in the time domain using a self-programmed code based on Crump’s method.The reliability of the obtained solutions is proved by the degeneration.Finally,the 2D plots of excess pore pressures and the curves of settlement varying with time,considering different physical parameters of unsaturated soil stratum and depth-dependent stress,are depicted and analyzed to study the 2D plane strain consolidation properties of unsaturated soils subjected to the depthdependent stress.
文摘Estimation of in situ stresses based on back-analysis of measured stress changes and displacements has become an alternative to the direct stress measurement methods.In order to help users conduct own measurement and analysis,this paper presents in detail a field stress back-analysis approach directly from borehole strain changes measured during nearby underground excavation.Essential formulations in major steps and the procedure for the entire analysis process are provided to allow users to follow.The instrument for borehole strain change measurement can be the CSIR or CSIRO stress cells and other borehole strain cells that can measure strains on borehole walls.Strain changes corresponding to the stress changes at a borehole location are calculated in borehole environment.The stress changes due to nearby excavation can be calculated by an analytical model for a single circular opening and simulated by a numerical model for non-circular and multiple openings.These models are based on isotropic,homogeneous and linear elastic assumptions.The analysis of borehole strain changes is accomplished by multiple linear regression based on error minimization and an integrated process provides the best-fit solution directly to the in situ stresses.A statistical technique is adopted for screening outliers in the measurement data,checking measurement compatibility and evaluating the reliability of analysis results.An application example is included to demonstrate the practical application and the analysis procedure.
文摘This paper analyzed the characteristics of welding solidification crack of stainless steels,and clearly re- vealed the the of the deformation in the molten - the pool and the solidification shrinkage on the stress - strain fields in the trail of molten - weld pool.Moreover, rheologic properties of the alloys in solid - liquid zone were also obtained by measuring the hading and unloading deform curves of the steels.As a result, a numerical model for simulation of stress - strain distributions of welding solidifi- cation crack was developed. On the basis of the model,the thesis simulated the driving force of solidifi- cation crack of stainless steels, that is, stress - strain fields in the trail of molten-weld pool with fi- nite element method.
文摘An analytical model for dynamic recrystallization (DRX) is studied based on the relative grain size model proposed by Sakai and Jonas, and the characteristic flow behaviors under DRX are analyzed and simulated. Introducing the variation of dynamic grain size and the heterogeneous distribution of disolo- cation densities densities under DRX,a simple method for modeling and simulating DRX processes is developed by using Laplace transformation theory. The results derived from the present model agree well with the experimental results in literatures. This simulation can reproduce a number of features in DRX flow behaviors, for example,single and multiple peak flow behaviors followed by a steady state flow, the transition between them, and so on.
文摘The mechanical behavior of plastic concrete used in the cut-off walls of earth dams has been studied.Triaxial compression tests on the specimens in various ages and mix designs under different confining pressures have been done and the stress-strain behavior of such materials and their strength parameter changes have been experimentally investigated.It has been observed that increasing the confining pressures applied on the specimens causes the material behavior to be alike the more ductile materials and the compressive strength increases considerably as well.Moreover,a parametric study has been carried out to investigate the influence of essential parameters on the shear strength parameters of these materials.According to the research,increasing the coarse to fine aggregates ratio leads to the increase of compressive strength of the specimens as well as the increase of the cohesion and internal friction angle of the materials.Furthermore,the bentonite content decrease and the cement factor increase result in an increase of the cohesion parameter of plastic concretes and decrease of the internal friction angle of such materials.
文摘A more accurate estimation of stress-strain relationships for martensite and ferrite was developed, and the modified law of mixture was used to investigate the stress-strain partitioning of constituent phases in dual phase （DP） steels with two different martensite volume fractions. The results show that there exist great differences in the stress-strain contribution of martensite and ferrite to DP steel. The stress-strain partitioning coefficient is not constant in the whole strain range, but decreases with increasing the true strain of DP steel. The softening effect caused by the dilution of carbon concentration in martensite with the increase of martensite volume fraction has great influence on the strain contribution of martensite. The strain ratio of ferrite to martensite almost linearly increases with increasing the true strain of DP steel when the martensite volume fraction is 22%, because martensite always keeps elastic. But the strain ratio of ferrite to martensite varies indistinctively with the further increase in true strain of DP steel above 0.034 when the martensite volume fraction is 50%, because plastic deformation happens in martensite. The stress ratio ofmartensite to ferrite decreases monotonously with increasing the true strain of DP steel whether the martensite volume fraction is 22% or 50%.
