Antibiotic resistance in gram-negative pathogens has become one of the most serious global public health threats.The role of the N-acyl homoserine lactone(AHL)-mediated signaling pathway,which is widespread in gram-ne...Antibiotic resistance in gram-negative pathogens has become one of the most serious global public health threats.The role of the N-acyl homoserine lactone(AHL)-mediated signaling pathway,which is widespread in gram-negative bacteria,in the bacterial resistance process should be studied in depth.Here,we report a degrading enzyme of AHLs,MomL,that inhibits the antibiotic resistance of Pseudomonas aeruginosa through a novel mechanism.The MomL-mediated reactivation of kanamycin is highly associated with the relA-mediated starvation stringent response.The degradation of AHLs by MomL results in the inability of LasR to activate relA,which,in turn,stops the activation of downstream rpoS.Further results show that rpoS directly regulates the type VI secretion system H2-T6SS.Under MomL treatment,inactivated RpoS fails to regulate H2-T6SS;therefore,the expression of effector phospholipase A is reduced,and the adaptability of bacteria to antibiotics is weakened.MomL in combination with kanamycin is effective against a wide range of gram-negative pathogenic bacteria.Therefore,this study reports a MomL-antibiotic treatment strategy on antibiotic-resistant bacteria and reveals its mechanism of action.展开更多
构建智慧水资源管理系统,提高水资源监测和管理能力是落实最严格水资源管理制度的有效技术措施之一。在深入分析水资源管理信息化建设需求、目标和内容的基础上,研制了智慧水资源管理系统。该系统由Web网站、移动APP、富客户端和三维数...构建智慧水资源管理系统,提高水资源监测和管理能力是落实最严格水资源管理制度的有效技术措施之一。在深入分析水资源管理信息化建设需求、目标和内容的基础上,研制了智慧水资源管理系统。该系统由Web网站、移动APP、富客户端和三维数字地球仿真模块构成,其核心业务模型和信息扩展接口利用百度地图API、NASA World Wind数字地球及微软WCF共享技术集成。智慧水资源管理是蚌埠市智慧水利的重要组成部分,为水资源管理决策提供专业、科学、准确的技术支持。该系统助力蚌埠市2014年、2015年和2016年在落实最严格水资源管理制度考核中取得了安徽省第一名,取得了较为明显的社会效益和经济效益。展开更多
基金the National Natural Science Foundation of China(Nos.42176108 and 31870023)the Young Taishan Scholars Program of Shandong Province(No.tsqn202103029)+2 种基金the Marine S&T Fund of Shandong Province for Pilot National Laboratory for Marine Science and Technology(Qingdao)(No.2018SDKJ0406-4)the Fundamental Research Funds for the Central Universities(No.201941009)the open research funds of the State Key Laboratory of Ophthalmology(No.303060202400368).
文摘Antibiotic resistance in gram-negative pathogens has become one of the most serious global public health threats.The role of the N-acyl homoserine lactone(AHL)-mediated signaling pathway,which is widespread in gram-negative bacteria,in the bacterial resistance process should be studied in depth.Here,we report a degrading enzyme of AHLs,MomL,that inhibits the antibiotic resistance of Pseudomonas aeruginosa through a novel mechanism.The MomL-mediated reactivation of kanamycin is highly associated with the relA-mediated starvation stringent response.The degradation of AHLs by MomL results in the inability of LasR to activate relA,which,in turn,stops the activation of downstream rpoS.Further results show that rpoS directly regulates the type VI secretion system H2-T6SS.Under MomL treatment,inactivated RpoS fails to regulate H2-T6SS;therefore,the expression of effector phospholipase A is reduced,and the adaptability of bacteria to antibiotics is weakened.MomL in combination with kanamycin is effective against a wide range of gram-negative pathogenic bacteria.Therefore,this study reports a MomL-antibiotic treatment strategy on antibiotic-resistant bacteria and reveals its mechanism of action.
文摘构建智慧水资源管理系统,提高水资源监测和管理能力是落实最严格水资源管理制度的有效技术措施之一。在深入分析水资源管理信息化建设需求、目标和内容的基础上,研制了智慧水资源管理系统。该系统由Web网站、移动APP、富客户端和三维数字地球仿真模块构成,其核心业务模型和信息扩展接口利用百度地图API、NASA World Wind数字地球及微软WCF共享技术集成。智慧水资源管理是蚌埠市智慧水利的重要组成部分,为水资源管理决策提供专业、科学、准确的技术支持。该系统助力蚌埠市2014年、2015年和2016年在落实最严格水资源管理制度考核中取得了安徽省第一名,取得了较为明显的社会效益和经济效益。
