The loaded matrix:neurotrophin-enriched hydrogels for stem cell brain repair in Parkinson's disease

More than 200 years after Parkinson's disease was first described by the English surgeon whose name would eventually be given to the condition,available treatments remain purely symptomatic,leaving a critical unmet clinical need for a diseasemodifying therapy.

A drug-loaded flexible substrate improves the performance of conformal cortical electrodes

Cortical electrodes are a powerful tool for the stimulation and/or recording of electrical activity in the nervous system.However,the inevitable wound caused by surgical implantation of electrodes presents bacterial infection and inflammatory reaction risks associated with foreign body exposure.Moreover,inflammation of the wound area can dramatically worsen in response to bacterial infection.These consequences can not only lead to the failure of cortical electrode implantation but also threaten the lives of patients.Herein,we prepared a hydrogel made of bacterial cellulose(BC),a flexible substrate for cortical electrodes,and further loaded antibiotic tetracycline(TC)and the anti-inflammatory drug dexamethasone(DEX)onto it.The encapsulated drugs can be released from the BC hydrogel and effectively inhibit the growth of Gram-negative and Gram-positive bacteria.Next,therapeutic cortical electrodes were developed by integrating the drug-loaded BC hydrogel and nine-channel serpentine arrays;these were used to record electrocorticography(ECoG)signals in a rat model.Due to the controlled release of TC and DEX from the BC hydrogel substrate,therapeutic cortical electrodes can alleviate or prevent symptoms associated with the bacterial infection and inflammation of brain tissue.This approach facilitates the development of drug delivery electrodes for resolving complications caused by implantable electrodes.

In situ formation of multiple catalysts for enhancing the hydrogen storage of MgH_(2) by adding porous Ni_(3)ZnC_(0.7)/Ni loaded carbon nanotubes microspheres

MgH_(2) is considered one of the most promising hydrogen storage materials because of its safety,high efficiency,high hydrogen storage quantity and low cost characteristics.But some shortcomings are still existed:high operating temperature and poor hydrogen absorption dynamics,which limit its application.Porous Ni_(3)ZnC_(0.7)/Ni loaded carbon nanotubes microspheres(NZC/Ni@CNT)is prepared by facile filtration and calcination method.Then the different amount of NZC/Ni@CNT(2.5,5.0 and 7.5 wt%)is added to the MgH_(2) by ball milling.Among the three samples with different amount of NZC/Ni@CNT(2.5,5.0 and 7.5 wt%),the MgH_(2)-5 wt%NZC/Ni@CNT composite exhibits the best hydrogen storage performances.After testing,the MgH_(2)-5 wt%NZC/Ni@CNT begins to release hydrogen at around 110℃ and hydrogen absorption capacity reaches 2.34 wt%H_(2) at 80℃ within 60 min.Moreover,the composite can release about 5.36 wt%H_(2) at 300℃.In addition,hydrogen absorption and desorption activation energies of the MgH_(2)-5 wt%NZC/Ni@CNT composite are reduced to 37.28 and 84.22 KJ/mol H_(2),respectively.The in situ generated Mg_(2)NiH_(4)/Mg_(2)Ni can serve as a"hydrogen pump"that plays the main role in providing more activation sites and hydrogen diffusion channels which promotes H_(2) dissociation during hydrogen absorption process.In addition,the evenly dispersed Zn and MgZn2 in Mg and MgH_(2) could provide sites for Mg/MgH_(2) nucleation and hydrogen diffusion channel.This attempt clearly proved that the bimetallic carbide Ni_(3)ZnC_(0.7) is a effective additive for the hydrogen storage performances modification of MgH_(2),and the facile synthesis of the Ni_(3)ZnC_(0.7)/Ni@CNT can provide directions of better designing high performance carbide catalysts for improving MgH_(2).

Prediction of the Pore-Pressure Built-Up and Temperature of Fire-Loaded Concrete with Pix2Pix

Concrete subjected to fire loads is susceptible to explosive spalling, which can lead to the exposure of reinforcingsteel bars to the fire, substantially jeopardizing the structural safety and stability. The spalling of fire-loaded concreteis closely related to the evolution of pore pressure and temperature. Conventional analytical methods involve theresolution of complex, strongly coupled multifield equations, necessitating significant computational efforts. Torapidly and accurately obtain the distributions of pore-pressure and temperature, the Pix2Pix model is adoptedin this work, which is celebrated for its capabilities in image generation. The open-source dataset used hereinfeatures RGB images we generated using a sophisticated coupled model, while the grayscale images encapsulate the15 principal variables influencing spalling. After conducting a series of tests with different layers configurations,activation functions and loss functions, the Pix2Pix model suitable for assessing the spalling risk of fire-loadedconcrete has been meticulously designed and trained. The applicability and reliability of the Pix2Pix model inconcrete parameter prediction are verified by comparing its outcomes with those derived fromthe strong couplingTHC model. Notably, for the practical engineering applications, our findings indicate that utilizing monochromeimages as the initial target for analysis yields more dependable results. This work not only offers valuable insightsfor civil engineers specializing in concrete structures but also establishes a robust methodological approach forresearchers seeking to create similar predictive models.

High Fe‑Loading Single‑Atom Catalyst Boosts ROS Production by Density Effect for Efficient Antibacterial Therapy

The current single-atom catalysts(SACs)for medicine still suffer from the limited active site density.Here,we develop a synthetic method capable of increasing both the metal loading and mass-specific activity of SACs by exchanging zinc with iron.The constructed iron SACs(h^(3)-FNC)with a high metal loading of 6.27 wt%and an optimized adjacent Fe distance of~4 A exhibit excellent oxidase-like catalytic performance without significant activity decay after being stored for six months and promising antibacterial effects.Attractively,a“density effect”has been found at a high-enough metal doping amount,at which individual active sites become close enough to interact with each other and alter the electronic structure,resulting in significantly boosted intrinsic activity of single-atomic iron sites in h^(3)-FNCs by 2.3 times compared to low-and medium-loading SACs.Consequently,the overall catalytic activity of h^(3)-FNC is highly improved,with mass activity and metal mass-specific activity that are,respectively,66 and 315 times higher than those of commercial Pt/C.In addition,h^(3)-FNCs demonstrate efficiently enhanced capability in catalyzing oxygen reduction into superoxide anion(O_(2)·^(−))and glutathione(GSH)depletion.Both in vitro and in vivo assays demonstrate the superior antibacterial efficacy of h^(3)-FNCs in promoting wound healing.This work presents an intriguing activity-enhancement effect in catalysts and exhibits impressive therapeutic efficacy in combating bacterial infections.

MiR-194-Loaded Gelatin Nanospheres Target MEF2C to Suppress Muscle Atrophy in Mechanical Unloading Model

There is still no effective therapy for muscle atrophy.It found that miR-194 was significantlydownregulated in muscle atrophy model.miR-194 could promote muscle differentiation,and also inhibit ubiquitin ligases.miR-194 loaded in gelatin nanosphere were injected into the muscle atrophy model to realize controlled release.

loaded question与语言的间接性

在大学英语教学中会碰到很多值得研究的问题，下面这篇文章就是一个在教学备课中碰到的问题。尽管每本词典对loaded question的解释、翻译不一，但无论采用哪一种意思，它在语用学上都体现了语言的间接性。本文通过举例说明想把loaded question给阐释清楚，以期能为同样使用《大学体验英语》这一教材的同行或对这一问题感兴趣的人提供一些参考。

Removal of cadmium using MnO_2 loaded D301 resin

MnO2 loaded weak basic anion exchange resin D301 （Anion exchange resin, macroreticular weak basic styrene） as adsorbent has been prepared and applied to the removal of cadmium. The adsorption characteristics have been investigated with respect to effect of pH, equilibrium isotherms, removal kinetic data, and interference of the coexisting ions. The results indicated that the Cd^2＋ could be efficiently removed using MnO2 loaded D301 resin in the pH range of 3-8 from aqueous solutions with the co-existence of high concentration of alkali and alkaline-earth metals ions. The saturate adsorption capacity of the Cd^2＋ was 77.88 mg/g. The adsorption process followed the pseudo first-order kinetics. The equilibrium data obtained in this study accorded excellently with the Langmuir adsorption isotherm.

On the Study of Translation Approaches of Culture-loaded Words in Literary Works

Culture-loaded words refer to expressions and idioms that carry typically culture messages. The peculiarity of one culture cannot be understood or accepted by readers from another culture. The uniqueness very likely makes misunderstanding and may lead to cultural vacancy in other cultures. Due to the culture differences between Chinese and English, it is hard for a translator to achieve complete equivalence at the level of word. This paper discusses the translation approaches of Chinese cultureloaded words in literary works.

Sensory innervation around immediately vs. delayed loaded implants: a pilot study

Although neurophysiological and psychophysical proof of osseoperception is accumulating, histomorphometric evidence for the neural mechanisms of functional compensation following immediate and delayed implant loading is still lacking. For this randomized split-mouth study, six mongrel dogs randomly received one of four treatment protocols at 36 implant-recipient sites over 16 weeks （third maxillary incisor, third and fourth mandibular premolar）： immediate implant placement and immediate loading （liP＋ IL）; delayed implant placement and delayed loading （DIP＋DL）; delayed implant placement and immediate loading （DIP＋IL）; and natural extraction socket healing （control）. Histomorphometry was performed in the peri-implant bone and soft tissues within 300 pm around the implants. Immunocytochemistry and transmission electron microscopy were used to confirm the presence of neural structures and to reveal their ultrastructural characteristics, respectively. Myelinated nerve fibres densely populated the peri-implant crestal gingival and apical regions, although they were also identified in the woven bone and in the osteons near the implant threads. Compared with the control group in the mandible, the group that received IIP＋IL showed a higher innervation （in N.mm^-2, 5.94±1.12 vs. 3.15±0.63, P〈0.001） and smaller fibre diameter （in pm, 1.37±0.05 vs. 1.64±0.13, P=0.016）, smaller axon diameter （in pm, 0.89±0.05 vs. 1.24±0,10, P=0.009） and g-ratio （0.64±0.04 vs. 0.76±0.05, P〈0.001） in the middle region around the implants. Compared with DIP＋IL in the mandible, IIP＋IL had a higher nerve density （in N.mm^-2, 13.23±2.54 vs. 9.64±1.86, P=0.027）, greater fibre diameter （in pm, 1.32±0.02 vs. 1.20±0.04, P=0.021）, greater axon diameter （in μm, 0.92±0.01 vs. 0.89±0.03, P=-0.035） and lower g-ratio （0.69±0.01 vs. 0.74±0.01, P=-0.033） in the apical region around the implants. It may be assumed that the treatment protocol with liP＋ IL is the preferred method to allow optimized peri-implant re-innervation, but further functional measurements are still required.

Investigation on Skidding of Rolling Element Bearing in Loaded Zone

Skidding which occurs when rolling element entering into the loaded zone is prone to cause wear and incipient failure to the raceways and rolling elements. This paper presents a dynamic model to investigate the skidding of a rolling element bearing under radial load when the rolling element is entering into the load zone. In this dynamic model, the effects of the contact forces, friction forces on the rolling element-race and rolling element-cage interfaces, gravity, and the centrifugal forces of rolling elements are taken into consideration. The Hertzian contact theory is applied to calculate the non-linear contact forces. The Coulomb friction law is used to calculate the friction forces. The differential equations of rotational motion of the rolling element with regard to its central axis and the central axis of the outer ring are established respectively. The dynamic equations are then solved by using a fourth-order Runge-Kutta algorithm. The skidding characteristics of rolling element at the entry into the loaded zone are exposed, and the effects of the operating parameters on skidding behavior are carefully investigated.

Loaded multi-tooth contact analysis and calculation for contact stress of face-gear drive with spur involute pinion

The analytical method based on "Hertz theory on normal contact of elastic solids" and the numerical method based on finite element method (FEM) calculating the contact stress of face-gear drive with spur involute pinion were introduced, and their relative errors are below 10%, except edge contact, which turns out that these two methods can compute contact stress of face-gear drive correctly and effectively. An agreement of the localized bearing contact stress is gotten for these two methods, making sure that the calculation results of FEM are reliable. The loaded meshing simulations of multi-tooth FEM model were developed, and the determination of the transmission error and the maximal load distribution factor of face-gear drive under torques were given. A formula for the maximal load distribution factor was proposed. By introducing the maximal load distribution factor in multi-tooth contact zone, a method for calculating the maximal contact stress in multi-tooth contact can be given. Compared to FEM, the results of these formulae are proved to be reliable, and the relative errors are below 10%.

Diagonally loaded SMI algorithm based on inverse matrix recursion

The derivation of a diagonally loaded sample-matrix inversion （LSMI） algorithm on the busis of inverse matrix recursion （i.e.LSMI-IMR algorithm） is conducted by reconstructing the recursive formulation of covariance matrix. For the new algorithm, diagonal loading is by setting initial inverse matrix without any addition of computation. In addition, a corresponding improved recursive algorithm is presented, which is low computational complexity. This eliminates the complex multiplications of the scalar coefficient and updating matrix, resulting in significant computational savings. Simulations show that the LSMI-IMR algorithm is valid.

Experimental investigation of axially loaded steel fiber reinforced high strength concrete-filled steel tube columns

An experimental study on the compressive behavior of steel fiber reinforced concrete-filled steel tube columns is presented. Specimens were tested to investigate the effects of the concrete strength, the thickness of steel tube and the steel fiber volume fraction on the ultimate strength and the ductility. The experimental results indicate that the addition of steel fibers in concrete can significantly improve the ductility and the energy dissipation capacity of the concrete-filled steel tube columns and delay the local buckling of the steel tube, but has no obvious effect on the failure mode. It has also been found that the addition of steel fibers is a more effective method than using thicker steel tube in enhancing the ductility, and more advantageous in the case of higher strength concrete. An analytical model to estimate the load capacity is proposed for steel tube columns filled with both plain concrete and steel fiber reinforced concrete. The predicted results are in good agreement with the experimental ones obtained in this work and literatures.

Use of antibiotic-loaded cement in total knee arthroplasty

Bone cement has the capacity to release antibiotic molecules if any antibiotic is included in it, and these elution properties are improved as cement porosity is increased. In vitro studies have shown high local antibiotic concentration for many hours or few days after its use. Antibiotic loaded bone cement(ALBC) is helpful when treating an infection in total knee arthroplasty(TKA) revision surgery. The purpose of this paper was to review the evidence for the routine use of ALBC in TKA in the literature, its pros and cons. Many authors have recommended the use of ALBC also in primary TKA for infection prophylaxis, but the evidence based on data from National Registries, randomized clinical trials and meta-analysis suggest a protective effect of ALBC against infection when used in hips, but not(or only mild) in knees. A possible explanation to this finding is that the duration and quantity of locally elevated antibiotic levels after surgery are smaller in TKA, due to the smaller amount of cement used for fixation in TKA-only a layer in the bone surface. There are some concerns about the routine use of ALBC in primary TKA as prophylaxis against infection: Firstly, there is a risk of hypersensivity or toxicity even when the chance is highly improbable. Secondly, there is a reduction in the mechanical properties of the cement, but this can be probably neglected if the antibiotic is used in low doses, not more than 1 g per 40 g cement package. Another significant concern is the increased economic cost, which could be overlooked if there were enough savings in treating fewer prosthetic infections. Finally, there is also a risk of selection of antibiotic-resistant strains of bacteria and this could be the main concern. If used, the choice of the antibiotic mixed in ALBC should consider microbiological aspects(broad antimicrobial spectrum and low rate of resistant bacteria), physical and chemical aspects(thermal stability, high water solubility), pharmacological characteristics(low risk to allergic reactions or toxicity) and economic aspects(not too expensive). The most commonly used antibiotics in ALBC are gentamicin, tobramycin and vancomycin. In conclusion, there is a paucity of randomized clinical trials in the use of ALBC in primary TKAs and the actual evidence of the effect of ALBC in reducing the risk of infection is insufficient. This, in addition to concerns about patient safety, risks of increase in the antibiotic resistance of microorganisms and the increase in costs in the procedure, lead us to recommend a cautious use of ALBC, perhaps only in high-risk patients(immunocompromised, morbidly obese, diabetic and patients with previous history of fracture or infection around the knee) unless the benefits of ALBC use were fully proven. Meanwhile, the rigorous use of peri-operative prophylactic systemic antibiotics and adoption of efficient antiseptic procedures and improved surgical techniques must be considered the gold standard in infection prevention in TKA surgery.

The Development of Highly Loaded Turbine Rotating Blades by Using 3D Optimization Design Method of Turbomachinery Blades Based on Artificial Neural Network & Genetic Algorithm

In order to improve turbine internal efficiency and lower manufacturing cost, a new highly loaded rotating blade has been developed. The 3D optimization design method based on artificial neural network and genetic algorithm is adopted to construct the blade shape. The blade is stacked by the center of gravity in radial direction with five sections. For each blade section, independent suction and pressure sides are constructed from the camber line using Bezier curves. Three-dimensional flow analysis is carried out to verify the performance of the new blade. It is found that the new blade has improved the blade performance by 0.5%. Consequently, it is verified that the new blade is effective to improve the turbine internal efficiency and to lower the turbine weight and manufacturing cost by reducing the blade number by about 15%.

Analysis and design of axially loaded piles in rock

Despite significant advancements in in situ test techniques,construction practices,understanding of rock joint and rock mass behaviours,and numerical analysis methods,the design of bored concrete cast-insitu piles in rock is still largely based on the assessment of bearing capacity.However,for many of the rock conditions encountered,the bearing capacity of piles is a nebulous concept and a figment of the designer’s imagination.Even if it can be reasonably quantified,it has little,if any,significance to the performance of a pile in rock.The load carrying capacity of even low strength rock(in most situations)is far in excess of the strength of the structure(for example,a building column)transmitting the load.Unsatisfactory performance of a pile in rock is usually a displacement issue and is a function of rock mass stiffness rather than rock mass strength.In addition,poor pile performance is much more likely to result from poor construction practices than excessive displacement of the rock mass.Exceptions occur for footings that are undermined,or where unfavourable structure in the rock allows movement towards a free surface to occur.Standards,codes of practices,reference books and other sources of design information should focus foundation design in rock on displacement rather than strength performance.Ground investigations should measure rock mass stiffness and defect properties,as well as intact rock strength.This paper summarises the fundamental concepts relating to performance of piles in rock and provides a basis for displacement focused design of piles in rock.It also presents comments relating to how piles are modelled in widely used commercial finite element software for soil-structure interaction analysis,within the context of the back-analysis of a pile load test,and proposes recommendations for pile analysis and design.

Simplified nonlinear theory of the dielectric loaded rectangular Cerenkov maser

To rapidly and accurately investigate the performance of the dielectric loaded rectangular Cerenkov maser, a simplified nonlinear theory is proposed, in which the variations of wave amplitude and wave phase are determined by two coupled first-order differential equations. Through combining with the relativistic equation of motion and adopting the forward wave assumption, the evolutions of the forward wave power, the power growth rate, the axial wave number, the accumulated phase offset, and the information of the particle movement can be obtained in a single-pass calculation. For an illustrative example, this method is used to study the influences of the beam current, the gap distance between the beam and the dielectric surface, and the momentum spread on the forward wave. The variations of the saturated power and the saturation length with the working frequency for the beams with different momentum spreads have also been studied. The result shows that the beam wave interaction is very sensitive to the electron beam state. To further verify this simplified theory, a comparison with the result produced from a rigorous method is also provided, we find that the evolution curves of the forward wave power predicted by the two methods exhibit excellent agreement. In practical applications, the developed theory can be used for the design and analysis of the rectangular Cerenkov maser.

Preparation and in vitro Release Properties of Mercaptopurine Drug-loaded Magnetic Microspheres

Magnetic Fe304 nanoparticles were synthesized by co-precipitation method and the mercaptopurine （MER） drug-loaded magnetic microspheres were obtained through emulsion cross-linking methods. The efficiency of this approach was evaluated in terms of drug loading content （DLC）, encapsulation efficiency （EE） and delivery properties in vitro, determined by high performance liquid chromatograph （HPLC）. The microspheres showed good DLC values of 11.8%, as well as good EE values of 79.4%. The in vitro drug release study was carried out in phosphate buffer solution （PBS） simulated body fluid, at 37 ~C with pH=7.4. The release profiles showed an initial fast release rate, which decreased as time progressed and about 84 % had been released after 48 h. The experimental results indicated that the prepared magnetic microspheres may be useful for potential applications of MER for magnetically targeted chemotherapy.

Separator coatings as efficient physical and chemical hosts of polysulfides for high-sulfur-loaded rechargeable lithium–sulfur batteries

Lithium-sulfur batteries(LSBs)are promising alternative energy storage devices to the commercial lithium-ion batteries.However,the LSBs have several limitations including the low electronic conductivity of sulfur(5×10^-30S cm^-1),associated lithium polysulfides(PSs),and their migration from the cathode to the anode.In this study,a separator coated with a Ketjen black(KB)/Nafion composite was used in an LSB with a sulfur loading up to 7.88 mg cm^-2to mitigate the PS migration.A minimum specific capacity(Cs)loss of 0.06%was obtained at 0.2 C-rate at a high sulfur loading of 4.39 mg cm^-2.Furthermore,an initial areal capacity up to 6.70 mAh cm^-2 was obtained at a sulfur loading of 7.88 mg cm^-2.The low Cs loss and high areal capacity associated with the high sulfur loading are attributed to the large surface area of the KB and sulfonate group(SO3^-)of Nafion,respectively,which could physically and chemically trap the PSs.