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Theoretically predicted innovative palladium stripe dopingcobalt(111) surface with excellent catalytic performance for carbonmonoxide oxidative coupling to dimethyl oxalate
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作者 Bingying Han Neng Shi +5 位作者 Mengjie Dong Ye Liu Runping Ye Lixia Ling Riguang Zhang Baojun Wang 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第9期235-243,共9页
Pd-based catalysts are extensively employed to catalyze CO oxidative coupling to generate DMO,while the expensive price and high usage of Pd hinder its massive application in industrial production.Designing Pd-based c... Pd-based catalysts are extensively employed to catalyze CO oxidative coupling to generate DMO,while the expensive price and high usage of Pd hinder its massive application in industrial production.Designing Pd-based catalysts with high efficiency and low Pd usage as well as expounding the catalytic mechanisms are significant for the reaction.In this study,we theoretically predict that Pd stripe doping Co(111)surface exhibits excellent performance than pure Pd(111),Pd monolayer supporting on Co(111)and Pd single atom doping Co(111)surface,and clearly expound the catalytic mechanisms through the density functional theory(DFT)calculation and micro-reaction kinetic model analysis.It is obtained that the favorable reaction pathway is COOCH_(3)-COOCH_(3)coupling pathway over these four catalysts,while the rate-controlling step is COOCH_(3)+CO+OCH_(3)→2COOCH_(3)on Pd stripe doping Co(111)surface,which is different from the case(2COOCH_(3)→DMO)on pure Pd(111),Pd monolayer supporting on Co(111)and Pd single atom doping Co(111)surface.This study can contribute a certain reference value for developing Pd-based catalysts with high efficiency and low Pd usage for CO oxidative coupling to DMO. 展开更多
关键词 CO oxidative coupling to DMO Pd stripe doping Co(111)surface Catalytic mechanism DFT calculation Micro-reaction kinetic model analysis Catalytic performance
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Physiological and Biochemical Characteristic of a Stripe Mutant with Abnormal Flower Organs in Rice 被引量:8
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作者 陈德西 马炳田 +2 位作者 王玉平 马玉清 李仕贵 《Agricultural Science & Technology》 CAS 2009年第4期15-18,共4页
[Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experiment... [Objective] The experiment aimed to explore physiological and biochemical changes of leaves after plants were mutated. [Method] A rice double mutant with stripes on stems, leaves and spikelets were taken as experimental materials to study the enzyme activity changes in different growth stages and amino acid variation in rice. [ Result] The SOD activity in mutant was higher than that in wild plant at tillering metaphase, but lower than that in wild type before heading stage and late flowering; the POD activity in three stages increased firstly then declined and the activity showed highest maximal activity at before heading stage. However, the POD activity in wild type showed the opposite change trend; the CAT activity presented degression at three stages, especially high at tillering metaphase, but reverse changes in wild type; the MDA activity decreased at three stages, but it was still higher than that in wild type, besides, the soluble sugar content of mutant was lower, but total amino acid content was increased. [ Conclusion] The expression of mutant characteristics was correlated with SOD, POD, CAT and MDA activity Changes and these changes made the mutant survive and rice quality change at last. 展开更多
关键词 RICE stripe Physiological and Biochemical Characteristic Rice quality
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Screening of Wheat Cultivars for High Temperature Resistance to Stripe Rust from Wheat Resources in Huanghuai Growth Area 被引量:4
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作者 杨炜迪 陈宏灏 王美南 《Agricultural Science & Technology》 CAS 2008年第3期89-91,共3页
[Objective] The study aimed to screen wheat cultivars with high temperature resistance to stripe rust from the wheat resources in Huanghuai growth area. [Method] Seedlings of 165 wheat cultivars from Huanghuai growth ... [Objective] The study aimed to screen wheat cultivars with high temperature resistance to stripe rust from the wheat resources in Huanghuai growth area. [Method] Seedlings of 165 wheat cultivars from Huanghuai growth area were identified by wheat stripe rust under high temperature; then the wheat cultivars showing stripe rust at seedling stage were further used to identify the same resistance in field. [Results] 13 cultivars were proved to be stripe rust resistant under high temperature, and the expression stages of stripe rust in the 13 cultivars were revealed. The field identification results confirmed the identification results at seedling stage via inoculation of mixed stripe rust of physiological races. The stripe resistances of wheat cultivars were also proved to be non-race-specific. [Conclusion] Wheat resources in Huanghuai growth area are abundant in wheat cultivars with high temperature resistance to stripe rust. 展开更多
关键词 WHEAT High temperature disease resistance stripe RUST
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Control of Wheat Stripe Rust and Ensuring High Yield of Crops under Planting of Wheat Variety Mixtures 被引量:3
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作者 沈丽 叶香萍 +4 位作者 廖华明 何华健 王胜 刘如东 罗林明 《Agricultural Science & Technology》 CAS 2008年第1期134-138,共5页
Wheat stripe rust has become the most dangerous disease which threaten safe yield of wheat in Sichuan Province. It is meaningful to provide technique support for integrated disease control by exploring the effective c... Wheat stripe rust has become the most dangerous disease which threaten safe yield of wheat in Sichuan Province. It is meaningful to provide technique support for integrated disease control by exploring the effective control measures of wheat stripe rust. Wheat stripe rust dynamic developments of all-planting and mixed-planting have been systematically investigated in this study by taking different mixed-planting combinations among 6 wheat varieties with different resistance levels. The results of this experiment show that the mixed-plantings of 4 and 6 wheat varieties can delay the occurance of wheat stripe rust,slow the speed of disease and decline the damage of disease as well as stabilize yield of wheats. 展开更多
关键词 Mixed-planting Wheat stripe rust CONTROL Stable yield
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Forecast Model for Occurrence Degree of Wheat Stripe Rust Using Meteorological Data 被引量:1
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作者 刘伟昌 陈怀亮 +3 位作者 王君 余卫东 张宏 刘忠阳 《Agricultural Science & Technology》 CAS 2008年第3期119-123,共5页
[Objective] The aim of this study is to establish the model for forecasting wheat stripe rust occurrence condition using meteorological factors. [Method] Based on the data of wheat stripe rust occurrence degrees in it... [Objective] The aim of this study is to establish the model for forecasting wheat stripe rust occurrence condition using meteorological factors. [Method] Based on the data of wheat stripe rust occurrence degrees in its past prevalent years and the meteorological data at corresponding periods, the methods of grey correlation analysis and fuzzy mathematics were employed to establish the forecast model for four pathogenesis indices according to the time sequence before winter, Early March, Early April and Middle May. Thus, the criterion for forecasting the occurrence degree of wheat stripe rust was obtained based on the distribution method of arithmetic progression. [Result] The model corresponding to meteorological conditions for forecasting wheat stripe rust was successfully established. According to the verification, the forecasting results before winter and in Early Mar. were more severer than the real occurrence condition, while the forecasting results in Early Apr. and Middle May were basically consistent with real values. [Conclusion] The results of the present study may avail the control of wheat stripe rust in Henan Province. 展开更多
关键词 Wheat stripe RUST GREY correlation Fuzzy MATHEMATICS PATHOGENESIS index
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异构盘阵中最优Stripe Unit Size选择技术 被引量:1
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作者 刘军 杨学军 +1 位作者 唐玉华 王勇献 《计算机学报》 EI CSCD 北大核心 2004年第6期819-827,共9页
缓解计算机IO子系统瓶颈的有效手段是采用磁盘阵列 .配置时 ,SUS(StripeUnitSize)的选择对盘阵性能的影响非常关键 ,而以往对异构盘阵的SUS的选择研究较少 .该文推导出了确定异构盘阵最优SUS的方程 ,具体工作如下 :首先 ,该文采用close... 缓解计算机IO子系统瓶颈的有效手段是采用磁盘阵列 .配置时 ,SUS(StripeUnitSize)的选择对盘阵性能的影响非常关键 ,而以往对异构盘阵的SUS的选择研究较少 .该文推导出了确定异构盘阵最优SUS的方程 ,具体工作如下 :首先 ,该文采用close队列网络模型来描述异构盘阵负载 ;然后 ,从该模型推导出系统数据传输率解析表达式 .根据该表达式和异构盘阵中设备的特性 ,可求出使传输率最大的SUS .最后 ,研究中进行了大量的模拟试验 .模拟试验表明在并发的进程数大于 10时 ,所推导的结论与模拟结果非常吻合 . 展开更多
关键词 异构盘阵 磁盘性能 最优stripe UNIT Size选择技术 磁盘阵列 close队列网络模型 模拟试验
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Molecular Detection of Resistance Genes to Stripe Rust and Powdery Mildew in Common Wheat Cultivar Yunmai52 被引量:1
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作者 李宏生 谭丽明 +7 位作者 赵红 吴绍柱 杨素梅 张志强 丁明亮 顾坚 杨木军 李绍祥 《Agricultural Science & Technology》 CAS 2016年第4期801-803,809,共4页
Yunmai52, developed by crossing with common wheat-Haynaldia villosa6AL/6VS translocation line 92R149 as a resistant parent in 1992, was a common wheat cultivar approved and released in 2007 in Yunnan Province, China, ... Yunmai52, developed by crossing with common wheat-Haynaldia villosa6AL/6VS translocation line 92R149 as a resistant parent in 1992, was a common wheat cultivar approved and released in 2007 in Yunnan Province, China, which is characterized by high resistance to powdery mildew and stripe rust. In this study,an F_2 population derived from a cross K78S/Yunmai52 was constructed to investigate the resistance genes, where K78 S is a wheat male sterile line susceptible to powdery mildew and stripe rust. Phenotypic identification of the parents, F_1 and F_2 populations and chi-square analyses showed that F_1 population was immune to stripe rust and powdery mildew; the segregation ratio of resistance and susceptibility to powdery mildew(χ~2=1.10χ~2_(1,0.05)=3.84) and stripe rust(χ~2=0.15χ~2_(1,0.05)=3.84) fit to a 3:1 ratio in F_2 population, indicating that Yunmai52 harbors a dominant stripe rust resistance gene and a dominant powdery mildew resistance gene. The individuals were further detected with a marker co-segregated with Pm21(SCAR_(1400)) and two markers closely linked with Yr26(XWe173 and Xbarc181). The results showed that polymorphic bands could be amplified between the parents and between resistance and susceptibility gene pools at the same locus. Randomly 96 individuals of F_2 population were selected for verification. The results showed that the phenotype was significantly correlated with the genotype. The detection accuracy of markers SCAR_(1400), XWe173 and Xbarc181 was 100%, 97.91% and 92.70%, respectively.Yunmai52 harbored powdery mildew resistance gene Pm21 and stripe rust resistance gene Yr26, which were both derived from 6AL/6VS translocation line 92R149.In addition, the results also demonstrate that Pm21 and Yr26 are two genes conferring durable resistance to powdery mildew and stripe rust in wheat. 展开更多
关键词 WHEAT Yunmai52 Powdery mildew stripe rust Resistance gene
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Study on Method of Removing Stripe Noise in CBERS-02 Image
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作者 王文英 周伟 +3 位作者 袁春 刘顺喜 周连芳 杨红磊 《Meteorological and Environmental Research》 CAS 2010年第1期42-45,共4页
Based on the project of land macroscopical monitoring by CBERS,a remote sensing image of Arongqi in Inner Mongolia was studied by different methods such as histogram matching,principal component analysis,moment matchi... Based on the project of land macroscopical monitoring by CBERS,a remote sensing image of Arongqi in Inner Mongolia was studied by different methods such as histogram matching,principal component analysis,moment matching,low-pass filter and wavelet transform.A qualitative analysis and quantitative assessment was also carried out.The results showed that wavelet transform could effectively remove stripe noise,and also kept its advantages in the details.Moment matching had a better strip removal,but it changed features in its spectrum easily and it was not fit for CBERS-02 image processing.Principal component analysis could not remove stripe noise,but also strengthened it in a certain extent. 展开更多
关键词 CBERS stripe noise stripe removing Moment matching Wavelet transform China
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Study on Epidemic Characteristics and Its Causes of Wheat Stripe Rust in Nanchong City 被引量:1
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作者 彭昌家 丁攀 +7 位作者 白体坤 冯礼斌 陈如胜 王婉秋 尹怀中 龙维国 肖立 文旭 《Agricultural Science & Technology》 CAS 2015年第2期292-297,共6页
On basis of the research result of stripe rust for 16 years since 1999,the epidemic characteristics and trend of stripe rust in the city were determined.Namely,the earlier the initial stage appeared,the heavier the di... On basis of the research result of stripe rust for 16 years since 1999,the epidemic characteristics and trend of stripe rust in the city were determined.Namely,the earlier the initial stage appeared,the heavier the disease would be.Furthermore,stripe rust has two introduction infection peaks,of which the first peak plays a key role.In farmlands,there are one to three epidemic peaks,and the infection area of the first peak plays the key role on the epidemic area of that year.In addition,the accumulated areas of late January was in significantly positive correlation with annually total area,with a correlation coefficient of 0.769 2.In recent 16 years,the frequency of severe stripe rust was as high as 81.25% which was 50% higher than that before 1995.The slight stripe rust became just in 2013,with a frequency of 6.3%,which indicated that the city has become a region hit by severe stripe rust.The internal reason is the reduction or loss of wheat variety's resistance to tripe rust for a new physiological race of rust is becoming pathogenic stronger and be the major race.Big fluctuation of temperatures in warm winter and spring,foggy and dew days slants much would be the external reason. 展开更多
关键词 WHEAT stripe rust Occurrence characteristics Epidemic rule WARNING Improvement
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镧系超导体中条纹相(stripe phase)的研究评述
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作者 路庆凤 杜保立 李喜贵 《河南师范大学学报(自然科学版)》 CAS CSCD 2004年第2期38-42,共5页
镧系超导体中,当掺杂空穴浓度等于1/8时出现的超导反常抑制现象一直是超导研究方面的一个焦点.J.M.Tranquada将La2-XSrXCuO4中出现的动态二唯无公度(incommensurate)自旋关联解释为电荷自旋相互分离的条纹相之后,人们对镧系超导体的电子... 镧系超导体中,当掺杂空穴浓度等于1/8时出现的超导反常抑制现象一直是超导研究方面的一个焦点.J.M.Tranquada将La2-XSrXCuO4中出现的动态二唯无公度(incommensurate)自旋关联解释为电荷自旋相互分离的条纹相之后,人们对镧系超导体的电子-电子、电子-晶格、以及磁相互作用进行了系统的研究.但条纹相形成的驱动机制以及条纹相与超导电性的关系等基本问题仍没有得到充分的解释. 展开更多
关键词 LMCO 高温超导 stripe PHASE 1/8超导反常现象
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Fast estimation of fundamental matrix based on stripe constraints
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作者 杨安康 刘京南 +1 位作者 内田敬久 内田悦行 《Journal of Southeast University(English Edition)》 EI CAS 2008年第4期473-477,共5页
In order to improve the performance of estimating the fundamental matrix, a key problem arising in stereo vision, a novel method based on stripe constraints is presented. In contrast to traditional methods based on al... In order to improve the performance of estimating the fundamental matrix, a key problem arising in stereo vision, a novel method based on stripe constraints is presented. In contrast to traditional methods based on algebraic least-square algorithms, the proposed approach aims to minimize a cost function that is derived from the minimum radius of the Hough transform. In a structured-light system with a particular stripe code pattern, there are linear constraints that the points with the same code are on the same surface. Using the Hough transform, the pixels with the same code map to the Hough space, and the radius of the intersections can be defined as the evaluation function in the optimization progress. The global optimum solution of the fundamental matrix can be estimated using a Levenberg- Marquardt optimization iterative process based on the Hough transform radius. Results illustrate the validity of this algorithm, and prove that this method can obtain good performance with high efficiency. 展开更多
关键词 fundamental matrix structured-light stripe code pattern stereo vision
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Screening of Rice Stripe Virus(RSV)-resistant Germplasm from Landraces of Rice(Oryza sativa L.)
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作者 虞利俊 范方军 +6 位作者 杨杰 王军 朱金燕 李文奇 唐玉邦 徐磊 仲维功 《Agricultural Science & Technology》 CAS 2014年第5期809-813,共5页
[Objective] The aim of this study was to screen rice strip virus (RSV)-resistant landraces. [Method] The resistance of 119 rice landraces to rice stripe virus was identified in field spontaneously infected with sma... [Objective] The aim of this study was to screen rice strip virus (RSV)-resistant landraces. [Method] The resistance of 119 rice landraces to rice stripe virus was identified in field spontaneously infected with smal plant-hopper. [Result] There were 55 landraces resistant to rice strip disease in 56 indica rice landraces, but on-ly two resistant to rice strip disease in 63 japonica rice landraces. [Conclusion] The results revealed that there were abundant rice landscapes resistant to RSV in Chi-na, and these varieties can be used to develop more genes resistant to RSV. 展开更多
关键词 Rice landrace Rice stripe virus Resistance identification
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(2+1)-维Boussinesq方程的lump解与lump-stripe混合解 被引量:1
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作者 庄建红 刘亚轻 郭金星 《北京信息科技大学学报(自然科学版)》 2017年第6期84-89,共6页
通过利用Hirota双线性形式,并借助符号计算Maple,得到了(2+1)-维Boussinesq方程的lump解、lump-stripe混合解、周期解与孤子解。通过选取不同的参数,并结合图像研究了这些解的动力学性质,特别是讨论了lump孤子和stripe孤子之间的相互作... 通过利用Hirota双线性形式,并借助符号计算Maple,得到了(2+1)-维Boussinesq方程的lump解、lump-stripe混合解、周期解与孤子解。通过选取不同的参数,并结合图像研究了这些解的动力学性质,特别是讨论了lump孤子和stripe孤子之间的相互作用现象,这些解及相关的性质将有利于理解(2+1)-维Boussinesq方程所描述的物理现象。 展开更多
关键词 (2+1)-维Boussinesq方程 HIROTA双线性方法 lump解 lump-stripe混合解
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Study on the control of rice stripe disease with Bilken virusicide
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作者 周辉 陈娟 《Agricultural Science & Technology》 CAS 2007年第2期21-26,共6页
The paper reviewed the function mechanism of Bilken virusicide against rice stipe disease, and then introduced its control effects in field test as well as its application method.
关键词 BUken virusicide rice stripe disease control effect
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基于Stripes的在线测试系统的设计与开发 被引量:1
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作者 郭超 《软件》 2013年第9期49-50,共2页
本文首先介绍了在线测试系统的研究意义及现存的问题,在经典测试理论的基础上,阐述了整个系统的设计思想、技术路线、开发平台的选择、系统总体框架、实现的功能以及具体的实现方法。以"大学英语"课程为例,对基于Stripes框架... 本文首先介绍了在线测试系统的研究意义及现存的问题,在经典测试理论的基础上,阐述了整个系统的设计思想、技术路线、开发平台的选择、系统总体框架、实现的功能以及具体的实现方法。以"大学英语"课程为例,对基于Stripes框架的在线测试系统进行了开发。 展开更多
关键词 stripeS 在线测试 外语教学
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Mapping stripe rust resistance genes by BSR-Seq:YrMM58 and YrHY1 on chromosome 2AS in Chinese wheat lines Mengmai 58 and Huaiyang 1 are Yr17 被引量:6
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作者 Yong Wang Huaizhi Zhang +15 位作者 Jingzhong Xie Bingmin Guo Yongxing Chen Huaiyu Zhang Ping Lu Qiuhong Wu Miaomiao Li Deyun Zhang Guanghao Guo Jian Yang Panpan Zhang Yan Zhang Xicheng Wang Hong Zhao Tingjie Cao Zhiyong Liu 《The Crop Journal》 SCIE CAS CSCD 2018年第1期91-98,共8页
Stripe rust(yellow rust), caused by Puccinia striiformis f. sp. tritici(PST),is one of the most devastating fungal diseases in common wheat(Triticum aestivum L.) in China and worldwide. Resistance breeding is the most... Stripe rust(yellow rust), caused by Puccinia striiformis f. sp. tritici(PST),is one of the most devastating fungal diseases in common wheat(Triticum aestivum L.) in China and worldwide. Resistance breeding is the most effective strategy to control diseases in crop plants. Chinese wheat lines Mengmai 58 and Huaiyang 1 are highly resistant to PST race CYR34(V26) at the adult plant stage. To genetically map the underlying resistance genes we developed segregating populations by crossing Mengmai 58 and Huaiyang 1 with the susceptible cultivar Nongda 399. The stripe rust resistances in Mengmai 58 and Huaiyang 1 were both controlled by single dominant genes, provisionally designated YrMM58 and YrHY1, respectively. Bulked segregant RNA-Seq(BSR-Seq) analysis showed that YrMM58 and YrHY1 were located in the same distal ~16 Mb region on chromosome 2 AS.Comparative genomics analysis with the physical map of Aegilops tauschii proved useful for developing additional markers to saturate the genetic linkage map. YrMM58 and YrHY1 were mapped to the distal end of chromosome arm 2 AS, with the closest marker WGGB148 being 7.7 cM and 3.8 cM from the resistance gene, which was considered to be Yr17. These markers can be used in marker-assisted selection. 展开更多
关键词 Genetic MAPPING stripe rust TRITICUM AESTIVUM BSR-seq SNP
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Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer 被引量:18
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作者 HuanJQ WeiZM 《Cell Research》 SCIE CAS CSCD 2001年第2期149-155,共7页
Immature embryos of rice varieties "Xiushui11" and "Chunjiang 11" precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticid... Immature embryos of rice varieties "Xiushui11" and "Chunjiang 11" precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant cant were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56%-72% measured as numbers of Geneticin (G418)-resistant calli produced and 36%-60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38%-61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16%-75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests. 展开更多
关键词 RICE Agrobacterium tumefaciens spider insecticidal gene transgenic plant Leaffolder striped stem borer insect bioassay.
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Effect of Rice Sowing Date on Occurrence of Small Brown Planthopper and Epidemics of Planthopper-Transmitted Rice Stripe Viral Disease 被引量:8
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作者 ZHU Jin-liang ZHU Zeng-rong +6 位作者 ZHOU Yin LU Qiang SUN Xiang-liang TAO Xian-guo CHEN Yue WANG Hua-di CHENG Jia-an 《Agricultural Sciences in China》 CAS CSCD 2009年第3期332-341,共10页
To understand the relationship between rice sowing date and occurrence of the rice small brown planthopper (SBPH) Laodelphax striatellus Fallen and the epidemics of the planthopper-transmitted rice stripe viral (RS... To understand the relationship between rice sowing date and occurrence of the rice small brown planthopper (SBPH) Laodelphax striatellus Fallen and the epidemics of the planthopper-transmitted rice stripe viral (RSV) disease, four sowing dates of rice were evaluated in 2006 and 2007. The results showed that the peak density of SBPH and RSV incidence in the nursery and in the transplanted field decreased with the delay of sowing date in single crop of japonica rice in north Zhejiang Province of China. The relationship between seedling RSV incidence at the end of the nursery trial with sowing date was well described by Weibull equation. The area under the curve of population dynamics (AUCPD or planthopper-day accumulation) or the peak density of the planthopper in the nursery could be summarized by a logistic equation. RSV incidence in the transplanted fields could be characterized quantitatively by a multivariate regression equation, including the variables of sowing date, peak density of the vector, and RSV incidence at the end of the nursery trial. That the descriptive model excluded the AUCPD in transplanted field implies that this variable is not necessary in forecasting disease epidemics in the field. The 2-year experiments sufficiently indicated that suitable sowing of rice could be used as one of the effective measures to control the vector population and therefore the planthopper-transmitted RSV on a larger scale. The optimal sowing date for the single-cropped transplanted japonica rice is recommended from late May to early June in north Zhejiang, China. 展开更多
关键词 RICE sowing date Laodelphax striatellus rice stripe viral disease EPIDEMICS
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LncRNAs are potentially involved in the immune interaction between small brown planthopper and rice stripe virus 被引量:4
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作者 CHEN Meng-yao YE Wan-yi +6 位作者 XIAO Hua-mei LI Mei-zhen CAO Zheng-hong YE Xin-hai ZHAO Xian-xin HE Kang LI Fei 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第12期2814-2822,共9页
Small brown planthopper(SBPH, Laodelphax striatellus Fallén) is an important vector of major crop pathogen rice stripe virus(RSV). Controlling SBPH population is an efficient approach to control RSV. Long non-cod... Small brown planthopper(SBPH, Laodelphax striatellus Fallén) is an important vector of major crop pathogen rice stripe virus(RSV). Controlling SBPH population is an efficient approach to control RSV. Long non-coding RNAs(lnc RNA) have been reported to block virus replication in hosts. However, the function of lnc RNAs in RSV infection and replication is still unknown. Here, we aimed to study regulatory mechanisms of lnc RNA in an immune system during RSV infection. First, lnc RNA genes were predicted from SBPH transcriptomes using a bioinformatics pipeline based on characteristics of lnc RNA. We identified 4 786 lnc RNA genes corresponding to 5 790 transcripts in SBPH from an RNA-Seq dataset of 15 transcriptomes. Differential expression analysis indicated that 3, 11, and 25 lnc RNA genes were highly expressed in gut, salivary gland, and ovary, respectively, of viruliferous SBPH(Student’s t-test, P<0.05). We randomly selected eight lnc RNAs for expression validation using quantitative real-time PCR, confirming the differential expression of these lnc RNAs between viruliferous and non-viruliferous SBPH. In summary, we present evidence that the expression of lnc RNA genes was induced by RSV infection, suggesting that RSV might be involved in the antivirus immune system in SBPH and participate in regulating the RSV replication mechanism. These data provide helpful information for future investigations of the interaction between lncRNA and RSV. 展开更多
关键词 lncRNA SMALL BROWN PLANTHOPPER rice stripe virus RNA-Seq VIRAL infection
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Cloning and Characterization of a Pathogenesis-Related Protein Gene TaPR10 from Wheat Induced by Stripe Rust Pathogen 被引量:5
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作者 ZHANG Gang LI Yi-min ZHANG Yi DONG Yan-ling WANG Xiao-jie WEI Guo-rong HUANG Li-li KANG Zhen-sheng 《Agricultural Sciences in China》 CAS CSCD 2010年第4期549-556,共8页
Pathogenesis-related proteins (PRs) play many important roles in plant defense response against pathogen attack. To better understand the molecular mechanism of PR genes involved in wheat adult plant resistance (AP... Pathogenesis-related proteins (PRs) play many important roles in plant defense response against pathogen attack. To better understand the molecular mechanism of PR genes involved in wheat adult plant resistance (APR) to stripe rust, based on a differentially expressed transcribed derived fragment (TDF), a novel PR gene from wheat cv. Xingzi 9104 infected by the Puccinia striiformis Westend f. sp. tritici Erikss. pathotype CY32, which was highly similar to the maize ZmPRIO gene and designated as TaPRIO, was identified using in silico cloning and RT-PCR method. This novel TaPRIO gene was predicted to encode a 160-amino acid protein with a deduced molecular weight of 17.06 kDa and an isoelectronic point (pI) of 5.19. An amino acid sequence analysis of TaPR10 demonstrated the presence of a typical conserved domain of pathogenesis related protein Bet v I family. Multiple alignment analysis based on the amino acids encoded by 10 different PRIO genes from maize (Zea mays), rice (Oryza sativa), broomcorn (Sorghum bicolor), and wheat (Triticum aestivum) indicated that PR proteins of class 10 was conserved among the 4 plant species with about 80% similarity. DNA sequence of TaPRIO suggested the presence of one 84-bp intron with the splicing sites of GT-AT bi-nucleotide sequence between 188 and 271 bp. Using a real-time quantitative RT-PCR (qRT-PCR), expression profiles of TaPRIO revealed that at the adult-plant stage, TaPRIO transcript was up-regulated as early as 12 h post-inoculation (hpi), with the occurrence of maximum induction at 24 hpi. At the seedling stage, TaPRIO was also slightly induced 18 hpi. However, the transcript amount was relatively lower than that of the adult-plant stage. Taken together, these results suggest that TaPRIO may participate in wheat defense response of APR to stripe rust. 展开更多
关键词 stripe rust pathogenesis-related protein expression profile gene cloning in silico cloning
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