BACKGROUND ： Some studies demonstrate that allogenic peripheral nerve segment embedded subcutaneously significantly reduce the infiltration of lymphocyte and decrease immunological reaction.OBJECTIVE ： To observe th...BACKGROUND ： Some studies demonstrate that allogenic peripheral nerve segment embedded subcutaneously significantly reduce the infiltration of lymphocyte and decrease immunological reaction.OBJECTIVE ： To observe the gross shape, optical and electron microscope results of allogenic nerve segment in rats 2 weeks after subcutaneous embedment, and compare with subcutaneous emdedment of autologous nerve segment. DESIGN ： A randomized and controlled experiment.SETTING ： Department of Orthopaedics of Fifth People＇s Hospital of Zhengzhou; Department of Orthopaedics,First Hospital Affiliated to Chongqing Medical University.MATERIALS ： Totally 30 adult healthy Wistar male rats, with body mass of （200±20） g, were enrolled. Ten rats were chosen as the donors of allogenic nerve transplantation. The other 20 rats were randomly divided into 2 groups： allogenic nerve embedment group and autologous nerve embedment group, with 10 rats in each one. JEM-1220 transmission electron microscope （Japan） and Olympus BX50 optical microscope （Japan） were used. METHODS ： This experiment was carried out at the laboratory of Orthopaedic Department, Chongqing Medical University from October 2000 to April 2002. ① Sciatic nerve of donor rats for allogenic nerve transplantation was cut off at 5 mm distant from pelvic strait.15 mm sciatic nerve segment was chosen from lateral part as graft, allogenic nerve embedment group： 15 mm sciatic nerve form the donor rats was embedded in the posterior part of right legs. Autologous nerve embedment group： 15 mm sciatic nerve segment of autologous left side was embedded in the posterior side of right legs. ② Nerve segment embedded subcutaneously was taken out at postoperative 2 weeks and performed gross observation; then 5 samples chosen randomly respectively from 2 groups and given haematoxylin-eosin staining and observation under optical microscope （×400）;The other 5 samples were made into ultrathin sections （0.5μm）and observed under transmission electron microscope（×17 000）. MAIN OUTCOME MEASURES ： Gross shape, optical and electron microscope results of nerve segments of rats between two groups at 2 weeks after subcutaneous embedment. RESULTS ： ① Results of gross observation： Appearance of nerve segment was similar between 2 groups. ② Results of optical observation： medullary sheath denaturation, axonotmesis, vascular engorgement, desmoplasia of adventitia and infiltration of inflammatory cells were all found in both 2 groups. Inflammatory reaction was a little more severe in the allogenic nerve embedment group than in the autologous nerve embedment groups.③Results of electron microscope ： Similar cataplasia and denaturation of medullary sheath and cataplasia of Schwann cell were all found in the 2 groups. CONCLUSION： Some inflammatory reaction occurs after allogenic nerve embedment, but the activity of Schwann cell is similar to that of peripheral nerve after autologous nerve embedment.展开更多
文摘BACKGROUND ： Some studies demonstrate that allogenic peripheral nerve segment embedded subcutaneously significantly reduce the infiltration of lymphocyte and decrease immunological reaction.OBJECTIVE ： To observe the gross shape, optical and electron microscope results of allogenic nerve segment in rats 2 weeks after subcutaneous embedment, and compare with subcutaneous emdedment of autologous nerve segment. DESIGN ： A randomized and controlled experiment.SETTING ： Department of Orthopaedics of Fifth People＇s Hospital of Zhengzhou; Department of Orthopaedics,First Hospital Affiliated to Chongqing Medical University.MATERIALS ： Totally 30 adult healthy Wistar male rats, with body mass of （200±20） g, were enrolled. Ten rats were chosen as the donors of allogenic nerve transplantation. The other 20 rats were randomly divided into 2 groups： allogenic nerve embedment group and autologous nerve embedment group, with 10 rats in each one. JEM-1220 transmission electron microscope （Japan） and Olympus BX50 optical microscope （Japan） were used. METHODS ： This experiment was carried out at the laboratory of Orthopaedic Department, Chongqing Medical University from October 2000 to April 2002. ① Sciatic nerve of donor rats for allogenic nerve transplantation was cut off at 5 mm distant from pelvic strait.15 mm sciatic nerve segment was chosen from lateral part as graft, allogenic nerve embedment group： 15 mm sciatic nerve form the donor rats was embedded in the posterior part of right legs. Autologous nerve embedment group： 15 mm sciatic nerve segment of autologous left side was embedded in the posterior side of right legs. ② Nerve segment embedded subcutaneously was taken out at postoperative 2 weeks and performed gross observation; then 5 samples chosen randomly respectively from 2 groups and given haematoxylin-eosin staining and observation under optical microscope （×400）;The other 5 samples were made into ultrathin sections （0.5μm）and observed under transmission electron microscope（×17 000）. MAIN OUTCOME MEASURES ： Gross shape, optical and electron microscope results of nerve segments of rats between two groups at 2 weeks after subcutaneous embedment. RESULTS ： ① Results of gross observation： Appearance of nerve segment was similar between 2 groups. ② Results of optical observation： medullary sheath denaturation, axonotmesis, vascular engorgement, desmoplasia of adventitia and infiltration of inflammatory cells were all found in both 2 groups. Inflammatory reaction was a little more severe in the allogenic nerve embedment group than in the autologous nerve embedment groups.③Results of electron microscope ： Similar cataplasia and denaturation of medullary sheath and cataplasia of Schwann cell were all found in the 2 groups. CONCLUSION： Some inflammatory reaction occurs after allogenic nerve embedment, but the activity of Schwann cell is similar to that of peripheral nerve after autologous nerve embedment.
