Since the time of Darwin,subspecies have been recognized as an initial stage in the evolution of species.However,the impact of dispersal on subspecies richness in birds globally has received little attention,despite d...Since the time of Darwin,subspecies have been recognized as an initial stage in the evolution of species.However,the impact of dispersal on subspecies richness in birds globally has received little attention,despite dispersal being a key factor in determining the rate of evolution and playing a significant role in evolutionary divergence.Therefore,it is important to conduct a comprehensive study to address this issue.In this study,we aimed to assess the association between subspecies richness(measured by the number of subspecies)and dispersal ability(measured by the hand-wing index)on a global scale,using a dataset of over 7000 bird species.Our results revealed a negative correlation between dispersal ability and the number of subspecies,without any quadratic correlation.The wing is a crucial phenotypic trait for birds,and the concept of subspecies is important in speciation theory and conservation biology.Therefore,our findings not only enhance our understanding of the association between avian morphological traits and evolutionary divergence but also have implications for the conservation of avian species diversity.展开更多
Salmonella enterica has a wide diversity,with numerous serovars belonging to six different subspecies with dynamic animal-host tropism.The FimH protein is the adhesin mediating binding to various cells,and slight amin...Salmonella enterica has a wide diversity,with numerous serovars belonging to six different subspecies with dynamic animal-host tropism.The FimH protein is the adhesin mediating binding to various cells,and slight amino acid discrepancy significantly affects the adherence capacities.To date,the general function of FimH variability across dif-ferent subspecies of Salmonella enterica has not been addressed.To investigate the biological functions of FimH among the six Salmonella enterica subspecies,the present study performed several assays to determine biofilm for-mation,Caenorhabditis elegans killing,and intestinal porcine enterocyte cell IPEC-J2 adhesion by using various FimH allele mutants.In general,allelic mutations in both the lectin and pilin domains of FimH could cause changes in bind-ing affnity,such as the N79S mutation.We also observed that the N79S variation in Salmonella Dublin increased the adhesive ability of IPEC-J2 cells.Moreover,a new amino acid substitution,T260M,within the pilin domain in one subspecies llb strain beneficial to binding to cells was highlighted in this study,even though the biofilm-forming and Caenorhabditis elegans-killing abilities exhibited no significant differences in variants.Combined with point muta-tions being a natural tendency due to positive selection in harsh environments,we speculate that allelic variation T26oM probably contributes to pathoadaptive evolution in Salmonella enterica subspecies llb.展开更多
AIM:To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis(MAP) in patients with inflammatory bowel disease [Crohn's disease(CD) and ulcerative colitis(UC)].METHODS:Thirty patients...AIM:To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis(MAP) in patients with inflammatory bowel disease [Crohn's disease(CD) and ulcerative colitis(UC)].METHODS:Thirty patients with CD(15 with at least one NOD2/CARD15 mutation),29 with UC,and 10 with no inflammatory bowel disease(IBD).were tested for MAP by polymerase chain reaction(specific IS900 fragment) and blood culture.RESULTS:MAP DNA was detected in all original blood samples and 8-wk blood cultures(CD,UC and non-IBD).Positive MAP DNA status was confirmed by dot blot assays.All 69 cultures were negative by acid-fast Ziehl-Neelsen staining.Viable MAP,in spheroplast form,was isolated from the 18-mo blood cultures of all 30 CD patients,one UC patient,and none of the non-IBD controls.No association was found between positive MAP cultures and use of immunosuppressive drugs or CDassociated single nucleotide polymorphisms.CONCLUSION:MAP is widely present in our area and MAP DNA can be recovered from the blood of CD,UC and non-IBD patients.However,MAP spheroplasts were only found in CD patients.展开更多
The genus Tropidophorus consists of small semi-aquatic lizards that dwell in lowland forest steams(Barbour,1921;Bauer&Jackman,2008).Here,we designate the neotype and re-describe T.guangxiensis Wen,1992 based on ne...The genus Tropidophorus consists of small semi-aquatic lizards that dwell in lowland forest steams(Barbour,1921;Bauer&Jackman,2008).Here,we designate the neotype and re-describe T.guangxiensis Wen,1992 based on newly collected topotypic specimens.We also describe a new subspecies from Xuefeng Mountain,Hongjiang County。展开更多
Background: The Cambodian Tailorbird(Orthotomus chaktomuk) was one of the most recent major ornithological discoveries in South-east Asia as it originated from lowland seasonally flooded scrub within the densely inhab...Background: The Cambodian Tailorbird(Orthotomus chaktomuk) was one of the most recent major ornithological discoveries in South-east Asia as it originated from lowland seasonally flooded scrub within the densely inhabited floodplain around the Tonle Sap, Mekong and Bassac rivers(Cambodia). The Cambodian Tailorbird is sister to the Dark-necked Tailorbird(O. atrogularis) with very limited genetics and biometric differentiation. Between 2004 and 2012, evidences of a new population of Ashy Tailorbird(O. ruficeps) in SE Cambodia/Vietnam accumulated but no museum specimens were ever reported. The Ashy Tailorbird currently consists of eight subspecies among which the closest geographical populations, O. r. cineraceus(SE Burma to Malay Peninsula, Sumatra, Bangka and Belitung) and O. r. borneoensis(Borneo), are allopatric. Subspecific identification of the Cambodian Ashy Tailorbirds individuals was not possible because of the limited differences in plumage among subspecies.Methods: We inspected the Orthotomus ruficeps specimens housed at the Museum National d'Histoire Naturelle(Paris) and discovered five specimens of O. ruficeps collected by Louis Rodolphe Germain in ‘Cochinchina'(corresponding to southern Vietnam) during the nineteenth century. We sequenced one mitochondrial locus and gathered biometric data from these specimens and compared them with other Orthotomus lineages.Results: The Ashy Tailord population from SE Cambodia and Vietnam is distinct from the two geographically close subspecies O. r. borneoensis(1.7%) and O. r. cineraceus(1.3%). O. chaktomuk is nested within O. atrogularis in the mitochondrial gene tree. The SE Cambodia/Vietnam population of O. ruficeps is distinct from the two other subspecies in bill shape.Conclusion: Our study described the biometric and molecular distinctiveness of a recently re-discovered population of Ashy Tailord in SE Cambodia and Vietnam and suggests that this population constitutes an independent evolutionary lineage that we describe here as a new subspecies. The newly described Cambodian Tailorbird is nested within the Dark-necked Tailorbird and the genetic divergence is much lower than initially described(0.4-0.7% vs 1.1-1.4%).展开更多
Mycobacterium avium subspecies paratuberculosis(MAP),originally called Johne’s bacillus was firstdescribed from Germany in 1895 as the cause of achronic inflammatory disease of the intestine in acow.As the 20th centu...Mycobacterium avium subspecies paratuberculosis(MAP),originally called Johne’s bacillus was firstdescribed from Germany in 1895 as the cause of achronic inflammatory disease of the intestine in acow.As the 20th century progressed,clinical andsub-clinical MAP infection in farm animals inWestern Europe appeared to become moreprevalent.Among the early reviews available展开更多
indica and japonica are the two most important subspecies of Asian cultivated rice. Identifying mechanisms responsible for population differentiation in these subspecies is important for indica-japonica hybridization ...indica and japonica are the two most important subspecies of Asian cultivated rice. Identifying mechanisms responsible for population differentiation in these subspecies is important for indica-japonica hybridization breeding. In this study, subspecies and economic trait differentiation patterns were analyzed using morphological and molecular (InDel and Intron Length Polymorphism) data in F2 and F3 populations derived from indica-japonica hybridization. Populations were grown in Liaoning and Guangdong provinces, China, with F3 populations generated from F2 populations using bulk harvesting (BM) and single-seed descent methods (SSD). Segregation distortion was detected in F3-BM populations, but not in F3- SSD or in F2 populations. Superior performance was observed with respect to economic traits in Liaoning compared with that in Guangdong and 1 000-grain weight (KW), seed setting rate (SSR) and grain yield per plant (GYP) were significantly correlated with indica and japonica subspecies types. Analysis of molecular and morphological data demonstrated that the environment is the main factor giving rise to population differentiation in indica-japonica hybridization. In addition, we also found that KW, SSR and GYP are related to subspecies characteristics and kinship, which is possibly a significant factor resulting in economic trait differentiation and determining environmental adaptability. Our study has provided new insights into the process of population differentiation in these subspecies to inform indica-japonica hybridization breeding.展开更多
Two recombinant inbred line (RIL) populations, one derived from a cross between Zhongyouzao 8 (indica) and Toyonishiki (japonica) and the other from a cross between Qishanzhan (indica) and Akihikari (japonica...Two recombinant inbred line (RIL) populations, one derived from a cross between Zhongyouzao 8 (indica) and Toyonishiki (japonica) and the other from a cross between Qishanzhan (indica) and Akihikari (japonica), were grown in Liaoning and Sichuan Provinces, China, to study the panicle type index (PTI, the ratio of a number of node position on panicle axis where the secondary branch with the most numerous secondary branch grains is located to the number of primary branches) and its relationships with subspecies characteristics and yield traits. With continuous distribution of PTI and numerous of lines with different PTIs in the progenies, indica-japonica hybridization could be a method for breeding cultivars with ideal panicle type. PTI was significantly influenced by environments, with a significant decrease from Liaoning to Sichuan A significantly negative correlation was found between PTI and most of subspecies characteristics. PTI varied remarkably with different subspecies types. As a whole, it showed a trend of indica (H)〉indicalinous (H')〉japonicalinous (K')〉japonica (K) However, it is not appropriate to regard PTI as a parameter for subspecies classification in rice because it was recombined in the filial generations of cross between indica and japonica rice. The negative correlations were found between PTI and most of panicle characteristics, as well as yield-related traits. Based on PTI, most of lines from the two populations were middle dominant panicle type (the number of grains on the secondary branch is numerous in middle position of the panicle) and lower dominant panicle type (the number of grains on the secondary branch is numerous in lower position of the panicle and becomes less towards the top of panicle) with significant yield advantages.展开更多
Based on extensive field work in the northern Zagros Mountains, a new subspecies of Darevskia raddei (Boettger, 1892) from the west Azerbaijan Province, northwestern Iran is described. The new subspecies is distingu...Based on extensive field work in the northern Zagros Mountains, a new subspecies of Darevskia raddei (Boettger, 1892) from the west Azerbaijan Province, northwestern Iran is described. The new subspecies is distinguishable from all the other three subspecies of D. raddei based on the combination of several distinguishing characters, such as dorsal body with 50-55 oval to round, smooth, or very weakly keeled, scales, 25-31 scales across the gular region, 18-20 femoral pores on each side of body (reaching the knee), 29-31 tuberculate lamellae under the fourth toe, 11-12 supraciliaries, 9-11 collars and a specific color pattern. The new subspecies meets all the morphological characters described by Eiselt et al. (1993) for an undefined and intermediate form between D. raddei raddei in the east of and D. r vanensis in the west of the distribution range of the species.展开更多
This study characterizes the 19 kDa protein expressed by Mycobacterium avium subspecies paratuberculosis (MAP) as a glycolipoprotein, providing the foundation for future experiments regarding its antigenicity and role...This study characterizes the 19 kDa protein expressed by Mycobacterium avium subspecies paratuberculosis (MAP) as a glycolipoprotein, providing the foundation for future experiments regarding its antigenicity and role in disease pathogenicity. We have previously shown that a 4.8 kb insert from MAP will produce a 16 kDa recombinant protein when expressed in Escherichia coli and 19 kDa recombinant protein when expressed in M. smegmatis (smeg19K). The difference of 3 kDa in size of these expressed proteins may be related to post translational modifications that occur in Mycobacterium species. We hypothesized that smeg19K is a glycolipoprotein since BLAST analysis revealed approximately 76% amino acid identity between the MAP 19 kDa protein and a known lipoglycoprotein, the 19 kDa protein of M. tuberculosis. This prediction was confirmed by the following positive staining of smeg19K with Sudan Black 4B, a postelectrophoresis dye used to stain for lipids. Smeg19K has also stained positively for glycosylation with the lectin concavalin A, a highly specific stain for mannose residues. As expected, treatment with tunicamycin (an antibiotic known to inhibit N-glycosylation) and treatment with deglycosylation assay (non-specific for mannose), showed no reduction in size of 19 kDa glycolipoprotein.展开更多
<span style="font-family:Verdana;">The White-breasted Thrasher (</span><i><i><span style="font-family:Verdana;">Ramphocinclus</span></i><span> <i&...<span style="font-family:Verdana;">The White-breasted Thrasher (</span><i><i><span style="font-family:Verdana;">Ramphocinclus</span></i><span> <i><span style="font-family:Verdana;">brachyurus</span></i><span></span></span></i><span style="font-family:Verdana;">) is the smallest passerine bird in the Mimids family.</span><span style="font-family:Verdana;"> Ranked as “endangered” in the IUCN list, the demographic distribution of this species is spread unevenly across two islands in the Lesser Antilles. The Saint-Lucian subspecies, <i></i></span><i><i><span style="font-family:Verdana;">Ramphocinclus</span></i><span> <i><span style="font-family:Verdana;">brachyurus</span></i> <i><span style="font-family:Verdana;">sanctaeluciae</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;">, was estimated to number between 1200 and 1700 individuals, whereas the Martinique subspecies, <i></i></span><i><i><span style="font-family:Verdana;">Ramphocinclus</span></i><span> <i><span style="font-family:Verdana;">brachyurus</span></i> <i><span style="font-family:Verdana;">brachyurus</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;">, was estimated at between 200 and 400 individuals. As an endemic rare species with a high risk of extinction, the Martinique subspecies should be regarded as a conservation priority. The small size of the Martinique subspecies population, its restricted geographical range, and its narrow habitat tolerance make it quite difficult to study. The variability of morphological traits in this subspecies has not been biostatistically analyzed recently. In</span><span style="font-family:Verdana;"> order to update knowledge and fill in any gaps, we have used the morphometric approach to study the morphological variability of the Martinique subspecies. The study site is a small peninsula located in the northeast of t</span><span style="font-family:Verdana;">he island of Martinique (French West Indies) known as “la Caravelle”. We captured 63 White-breasted Thrashers which were marked, measured, and sexed before being released. We did not observe any significant differences betwe</span><span style="font-family:Verdana;">en the sexes, signifying an absence of sexual dimorphism. However, we were able to observe a significant variation in morphological traits between individuals measured inside and outside the national protected area of Ca</span><span style="font-family:Verdana;">ravelle. The body mass-tarsus ratio was equal at both sampled sites, something which could potentially be a clue to the bird’s adaptation to different habitats.</span>展开更多
Streptococcus dysgalactiae subspecies equisimilis (SDSE) is a β-hemolytic Streptococcus that possesses genetic and clinical similarities to Streptococcus pyogenes. It is increasingly recognized as the etiological mic...Streptococcus dysgalactiae subspecies equisimilis (SDSE) is a β-hemolytic Streptococcus that possesses genetic and clinical similarities to Streptococcus pyogenes. It is increasingly recognized as the etiological microorganism of invasive diseases. We report a case of a 74-year-old male who was admitted to this hospital with lower back and neck pain and infected with leg ulcer. The diagnosis of spondylodiscitis C2-C3 and L1-L3 caused by Streptococcus dysgalactiae subsp. equisimilis was made. The present case demonstrates the risk of older patients of developing invasive disease upon skin infection with Streptococcus dysgalactiae subsp. equisimilis, even when risk factors are absent or well controlled (as was diabetes mellitus in this case), suggesting that the pathogenic potential of SDSE should not be underestimated.展开更多
The cultivated rice(Oryza sativa L.)is known to contain two major subspecies,indica(O. stativa L. ssp. indica)and japonica(O. sativa L. ssp.japonica). The indica and japonica differentiation
BACKGROUND Streptococcus gallolyticus subspecies pasteurianus(SGSP)is a rare pathogen responsible for infant sepsis and meningitis and is potentially overlooked because it is not included in routine group B streptococ...BACKGROUND Streptococcus gallolyticus subspecies pasteurianus(SGSP)is a rare pathogen responsible for infant sepsis and meningitis and is potentially overlooked because it is not included in routine group B streptococcal screenings.Hence,we present a case of SGSP-induced infant meningitis and sepsis,accompanied by bronchopneumonia induced by multidrug-resistant Staphylococcus aureus(MRSA),providing insights into the identification,management,and prognosis of this bacterial infection.CASE SUMMARY A 45-day-old female infant presented with two episodes of high fever(maximum temperature:39.5°C)and two generalized grand mal seizure episodes that lasted over ten seconds and self-resolved without concomitant symptoms.Postadmission,the patient’s C-reactive protein level was 40.73 mg/L,white blood cell count was 13.42×10^(9)/L,neutrophil ratio was 78.4%,procalcitonin level was 7.89μg/L,cerebrospinal fluid(CSF)white cell count was 36×10^(6)/L,multinucleated cell ratio was 95.2%,and protein concentration was 0.41 g/L.Blood and CSF culture revealed that the pathogen was SGSP.The bacterium was sensitive to ampicillin,furazolidone,penicillin,lincomycin,moxifloxacin,rifampicin,vancomycin,and levofloxacin but resistant to clindamycin and tetracycline.Sputum culture revealed the presence of MRSA,which was sensitive to vancomycin.The patient was diagnosed with meningitis and sepsis caused by SGSP,accompanied by bronchopneumonia induced by MRSA.Ceftriaxone(100 mg/kg/d)combined with vancomycin(10 mg/kg/dose,q6h)was given as an anti-infective treatment postadmission.After 12 days of treatment,the infant was discharged from the hospital with normal CSF,blood culture,and routine blood test results,and no complications,such as subdural effusion,were observed on cranial computed tomography.No growth retardation or neurological sequelae occurred during follow-up.CONCLUSION SGPSP-induced infant bacterial meningitis and sepsis should be treated with prompt blood and CSF cultures,and a sensitive antibiotic therapy to ensure a favorable prognosis.展开更多
Understanding the biological parameters of some triatomine subspecies of Meccus phyllosomus (Burmeister) is a crucial first step in estimating the epidemiologie importance of this group. Biological parameters relate...Understanding the biological parameters of some triatomine subspecies of Meccus phyllosomus (Burmeister) is a crucial first step in estimating the epidemiologie importance of this group. Biological parameters related to hatching, lifetime, number of blood meals to molt, percentage of females at the end of the cycle, number of laid eggs, and mortality for each instar of 3 M. phyllosomus subspecies [M. p. mazzottii (Usinger), M. p. pallidipennis (Stal), and M. p. phyllosomus] and their laboratory hybrids were evaluated and compared. No significant differences (P 〉 0.05) were found among the experimental hybrids (MaPa, MaPhy, PaPhy) and reciprocal cohorts. In 5 (hatching, number of blood meals to molt, accumulative mortality, percentage of females, and mean number of laid eggs) of the 6 studied parameters (with the exception of development time), the hybrid cohorts had better fitness results than the parental cohorts involved in each set of crosses. The increase in hybrid fitness found in our study could lead to an increase in the epidemiologic risks caused by transmission of Trypanosoma cruzi to humans.展开更多
Pheromonal communication plays a key role in the sociosexual behavior of rodents.The coadaptation between pheromones and chemosensory systems has been well illustrated in insects but poorly investigated in rodents and...Pheromonal communication plays a key role in the sociosexual behavior of rodents.The coadaptation between pheromones and chemosensory systems has been well illustrated in insects but poorly investigated in rodents and other mammals.We aimed to investigate whether coadaptation between male pheromones and female reception might have occurred in brown rats Rattus norve-gicus.We recently reported that major urinary protein(MUP)pheromones are associated with male mating success in a brown rat subspecies,R.n.humiliatus(Rnh).Here,we discovered that MUPs were less polymorphic and occurred at much lower concentrations in males of a parapatric subspecies,R.n.caraco(Rnc),than in Rnh males,and found no association between pheromones and paternity success.Moreover,the observation of Rnc males that experienced chronic dyadic encounters and established dominance-submission relationships revealed that the dominant males achieved greater mating success than the subordinate males,but their MUP levels did not differ by social status.These findings suggest that male mating success in Rnc rats is related to social rank rather than to pheromone levels and that low concentration of MUPs might not be a reliable signal for mate choice in Rnc rats,which is different from the findings obtained in Rnh rats.In addition,compared with Rnh females,Rnc females exhibited reduced expression of pheromone receptor genes,and a lower number of vomeronasal receptor neurons were activated by MUP pheromones,which imply that the female chemosensory reception of pheromones might be structurally and functionally coadapted with male pheromone signals in brown rats.展开更多
Having reviewed the major classification systems proposed by various scholars across the world, it is found that \%indica\% and \%japonica\% under \%O.sativa\% L. are two major directions thoroughly differentiated fro...Having reviewed the major classification systems proposed by various scholars across the world, it is found that \%indica\% and \%japonica\% under \%O.sativa\% L. are two major directions thoroughly differentiated from the Asian cultivated rice, forming the framework of the classification structure. A system with \%indica\% and \%japonica\% as the only two subspecies is therefore reiterated.There are various ways to determine the indica_japonica identity of hybrid rice, but the "combined morphological trait index"(CMT index) method is more efficient and easier to handle, although the isozyme analysis, molecular marker analysis and grain quality assay methods are also feasible.展开更多
Exploring the indica-japonica differentiation in parents of hybridization can provide theoretical bases for utilizing inter-subspecific heterosis. In this study, 5 sterile lines and 18 self-bred restorer lines were us...Exploring the indica-japonica differentiation in parents of hybridization can provide theoretical bases for utilizing inter-subspecific heterosis. In this study, 5 sterile lines and 18 self-bred restorer lines were used as female parents and male parents respectively. Then 90 combinations were constructed by incomplete diallel cross followed by relationship analysis between parental Cheng’s index difference value and Euclidean distance and heterosis. The results showed a significant correlation between several phenotype values, super male parent heterosis and control heterosis and Euclidean distance or Cheng’s index difference value. However, it was no significant correlation for yield. Further analysis found a common interval, 3.41 - 3.46 for Euclidean distance and 3 - 4 for cheng’s index difference value of parents, which was significant or high significant positive correlated with phenotype value, super male parent and control heterosis of main yield traits. This illustrates that the larger the genetic difference of parents was, the stronger the heterosis combinations were, when the genetic differences of parents were in an appropriate range.展开更多
基金Financial support for this study was provided by the National Natural Science Foundation of China(Grant No.31901084,32200406,32211530420).
文摘Since the time of Darwin,subspecies have been recognized as an initial stage in the evolution of species.However,the impact of dispersal on subspecies richness in birds globally has received little attention,despite dispersal being a key factor in determining the rate of evolution and playing a significant role in evolutionary divergence.Therefore,it is important to conduct a comprehensive study to address this issue.In this study,we aimed to assess the association between subspecies richness(measured by the number of subspecies)and dispersal ability(measured by the hand-wing index)on a global scale,using a dataset of over 7000 bird species.Our results revealed a negative correlation between dispersal ability and the number of subspecies,without any quadratic correlation.The wing is a crucial phenotypic trait for birds,and the concept of subspecies is important in speciation theory and conservation biology.Therefore,our findings not only enhance our understanding of the association between avian morphological traits and evolutionary divergence but also have implications for the conservation of avian species diversity.
基金supported by the National Program on Key Research Project of China(2022YFC2604201)well as the European Union's Horizon 2020 Research and Innovation Programme under Grant Agreement No.861917-SAFFl,Zhejiang Provincial Key R&D Program of China(2023C03045)+2 种基金Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City(2021JJLH0083)Key Research and Development Program of Hangzhou(202203A08)District-level project for high-level innovative and entrepreneurial talents of"Zijinshan Talents Gaochun Plan"(202100677).
文摘Salmonella enterica has a wide diversity,with numerous serovars belonging to six different subspecies with dynamic animal-host tropism.The FimH protein is the adhesin mediating binding to various cells,and slight amino acid discrepancy significantly affects the adherence capacities.To date,the general function of FimH variability across dif-ferent subspecies of Salmonella enterica has not been addressed.To investigate the biological functions of FimH among the six Salmonella enterica subspecies,the present study performed several assays to determine biofilm for-mation,Caenorhabditis elegans killing,and intestinal porcine enterocyte cell IPEC-J2 adhesion by using various FimH allele mutants.In general,allelic mutations in both the lectin and pilin domains of FimH could cause changes in bind-ing affnity,such as the N79S mutation.We also observed that the N79S variation in Salmonella Dublin increased the adhesive ability of IPEC-J2 cells.Moreover,a new amino acid substitution,T260M,within the pilin domain in one subspecies llb strain beneficial to binding to cells was highlighted in this study,even though the biofilm-forming and Caenorhabditis elegans-killing abilities exhibited no significant differences in variants.Combined with point muta-tions being a natural tendency due to positive selection in harsh environments,we speculate that allelic variation T26oM probably contributes to pathoadaptive evolution in Salmonella enterica subspecies llb.
基金Supported by A grant from Fundación de Investigación Médica Mutua Madrilena(FMM)(to Díaz-Rubio M)
文摘AIM:To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis(MAP) in patients with inflammatory bowel disease [Crohn's disease(CD) and ulcerative colitis(UC)].METHODS:Thirty patients with CD(15 with at least one NOD2/CARD15 mutation),29 with UC,and 10 with no inflammatory bowel disease(IBD).were tested for MAP by polymerase chain reaction(specific IS900 fragment) and blood culture.RESULTS:MAP DNA was detected in all original blood samples and 8-wk blood cultures(CD,UC and non-IBD).Positive MAP DNA status was confirmed by dot blot assays.All 69 cultures were negative by acid-fast Ziehl-Neelsen staining.Viable MAP,in spheroplast form,was isolated from the 18-mo blood cultures of all 30 CD patients,one UC patient,and none of the non-IBD controls.No association was found between positive MAP cultures and use of immunosuppressive drugs or CDassociated single nucleotide polymorphisms.CONCLUSION:MAP is widely present in our area and MAP DNA can be recovered from the blood of CD,UC and non-IBD patients.However,MAP spheroplasts were only found in CD patients.
文摘The genus Tropidophorus consists of small semi-aquatic lizards that dwell in lowland forest steams(Barbour,1921;Bauer&Jackman,2008).Here,we designate the neotype and re-describe T.guangxiensis Wen,1992 based on newly collected topotypic specimens.We also describe a new subspecies from Xuefeng Mountain,Hongjiang County。
基金support by a Synthesys grant made available to J.Fuchs by the European Community-Research Infrastructure Action under the FP6 Structuring the European Research Area Program(SE-TAF-7153)
文摘Background: The Cambodian Tailorbird(Orthotomus chaktomuk) was one of the most recent major ornithological discoveries in South-east Asia as it originated from lowland seasonally flooded scrub within the densely inhabited floodplain around the Tonle Sap, Mekong and Bassac rivers(Cambodia). The Cambodian Tailorbird is sister to the Dark-necked Tailorbird(O. atrogularis) with very limited genetics and biometric differentiation. Between 2004 and 2012, evidences of a new population of Ashy Tailorbird(O. ruficeps) in SE Cambodia/Vietnam accumulated but no museum specimens were ever reported. The Ashy Tailorbird currently consists of eight subspecies among which the closest geographical populations, O. r. cineraceus(SE Burma to Malay Peninsula, Sumatra, Bangka and Belitung) and O. r. borneoensis(Borneo), are allopatric. Subspecific identification of the Cambodian Ashy Tailorbirds individuals was not possible because of the limited differences in plumage among subspecies.Methods: We inspected the Orthotomus ruficeps specimens housed at the Museum National d'Histoire Naturelle(Paris) and discovered five specimens of O. ruficeps collected by Louis Rodolphe Germain in ‘Cochinchina'(corresponding to southern Vietnam) during the nineteenth century. We sequenced one mitochondrial locus and gathered biometric data from these specimens and compared them with other Orthotomus lineages.Results: The Ashy Tailord population from SE Cambodia and Vietnam is distinct from the two geographically close subspecies O. r. borneoensis(1.7%) and O. r. cineraceus(1.3%). O. chaktomuk is nested within O. atrogularis in the mitochondrial gene tree. The SE Cambodia/Vietnam population of O. ruficeps is distinct from the two other subspecies in bill shape.Conclusion: Our study described the biometric and molecular distinctiveness of a recently re-discovered population of Ashy Tailord in SE Cambodia and Vietnam and suggests that this population constitutes an independent evolutionary lineage that we describe here as a new subspecies. The newly described Cambodian Tailorbird is nested within the Dark-necked Tailorbird and the genetic divergence is much lower than initially described(0.4-0.7% vs 1.1-1.4%).
文摘Mycobacterium avium subspecies paratuberculosis(MAP),originally called Johne’s bacillus was firstdescribed from Germany in 1895 as the cause of achronic inflammatory disease of the intestine in acow.As the 20th century progressed,clinical andsub-clinical MAP infection in farm animals inWestern Europe appeared to become moreprevalent.Among the early reviews available
基金supported by the National Natural Science Foundation of China (30971845)
文摘indica and japonica are the two most important subspecies of Asian cultivated rice. Identifying mechanisms responsible for population differentiation in these subspecies is important for indica-japonica hybridization breeding. In this study, subspecies and economic trait differentiation patterns were analyzed using morphological and molecular (InDel and Intron Length Polymorphism) data in F2 and F3 populations derived from indica-japonica hybridization. Populations were grown in Liaoning and Guangdong provinces, China, with F3 populations generated from F2 populations using bulk harvesting (BM) and single-seed descent methods (SSD). Segregation distortion was detected in F3-BM populations, but not in F3- SSD or in F2 populations. Superior performance was observed with respect to economic traits in Liaoning compared with that in Guangdong and 1 000-grain weight (KW), seed setting rate (SSR) and grain yield per plant (GYP) were significantly correlated with indica and japonica subspecies types. Analysis of molecular and morphological data demonstrated that the environment is the main factor giving rise to population differentiation in indica-japonica hybridization. In addition, we also found that KW, SSR and GYP are related to subspecies characteristics and kinship, which is possibly a significant factor resulting in economic trait differentiation and determining environmental adaptability. Our study has provided new insights into the process of population differentiation in these subspecies to inform indica-japonica hybridization breeding.
基金supported by the National Natural Science Foundation of China(GrantNo.30871468)the Young Teacher Scientific Research Fund of Shenyang Agricultural University,China(Grant No.20081001)
文摘Two recombinant inbred line (RIL) populations, one derived from a cross between Zhongyouzao 8 (indica) and Toyonishiki (japonica) and the other from a cross between Qishanzhan (indica) and Akihikari (japonica), were grown in Liaoning and Sichuan Provinces, China, to study the panicle type index (PTI, the ratio of a number of node position on panicle axis where the secondary branch with the most numerous secondary branch grains is located to the number of primary branches) and its relationships with subspecies characteristics and yield traits. With continuous distribution of PTI and numerous of lines with different PTIs in the progenies, indica-japonica hybridization could be a method for breeding cultivars with ideal panicle type. PTI was significantly influenced by environments, with a significant decrease from Liaoning to Sichuan A significantly negative correlation was found between PTI and most of subspecies characteristics. PTI varied remarkably with different subspecies types. As a whole, it showed a trend of indica (H)〉indicalinous (H')〉japonicalinous (K')〉japonica (K) However, it is not appropriate to regard PTI as a parameter for subspecies classification in rice because it was recombined in the filial generations of cross between indica and japonica rice. The negative correlations were found between PTI and most of panicle characteristics, as well as yield-related traits. Based on PTI, most of lines from the two populations were middle dominant panicle type (the number of grains on the secondary branch is numerous in middle position of the panicle) and lower dominant panicle type (the number of grains on the secondary branch is numerous in lower position of the panicle and becomes less towards the top of panicle) with significant yield advantages.
文摘Based on extensive field work in the northern Zagros Mountains, a new subspecies of Darevskia raddei (Boettger, 1892) from the west Azerbaijan Province, northwestern Iran is described. The new subspecies is distinguishable from all the other three subspecies of D. raddei based on the combination of several distinguishing characters, such as dorsal body with 50-55 oval to round, smooth, or very weakly keeled, scales, 25-31 scales across the gular region, 18-20 femoral pores on each side of body (reaching the knee), 29-31 tuberculate lamellae under the fourth toe, 11-12 supraciliaries, 9-11 collars and a specific color pattern. The new subspecies meets all the morphological characters described by Eiselt et al. (1993) for an undefined and intermediate form between D. raddei raddei in the east of and D. r vanensis in the west of the distribution range of the species.
文摘This study characterizes the 19 kDa protein expressed by Mycobacterium avium subspecies paratuberculosis (MAP) as a glycolipoprotein, providing the foundation for future experiments regarding its antigenicity and role in disease pathogenicity. We have previously shown that a 4.8 kb insert from MAP will produce a 16 kDa recombinant protein when expressed in Escherichia coli and 19 kDa recombinant protein when expressed in M. smegmatis (smeg19K). The difference of 3 kDa in size of these expressed proteins may be related to post translational modifications that occur in Mycobacterium species. We hypothesized that smeg19K is a glycolipoprotein since BLAST analysis revealed approximately 76% amino acid identity between the MAP 19 kDa protein and a known lipoglycoprotein, the 19 kDa protein of M. tuberculosis. This prediction was confirmed by the following positive staining of smeg19K with Sudan Black 4B, a postelectrophoresis dye used to stain for lipids. Smeg19K has also stained positively for glycosylation with the lectin concavalin A, a highly specific stain for mannose residues. As expected, treatment with tunicamycin (an antibiotic known to inhibit N-glycosylation) and treatment with deglycosylation assay (non-specific for mannose), showed no reduction in size of 19 kDa glycolipoprotein.
文摘<span style="font-family:Verdana;">The White-breasted Thrasher (</span><i><i><span style="font-family:Verdana;">Ramphocinclus</span></i><span> <i><span style="font-family:Verdana;">brachyurus</span></i><span></span></span></i><span style="font-family:Verdana;">) is the smallest passerine bird in the Mimids family.</span><span style="font-family:Verdana;"> Ranked as “endangered” in the IUCN list, the demographic distribution of this species is spread unevenly across two islands in the Lesser Antilles. The Saint-Lucian subspecies, <i></i></span><i><i><span style="font-family:Verdana;">Ramphocinclus</span></i><span> <i><span style="font-family:Verdana;">brachyurus</span></i> <i><span style="font-family:Verdana;">sanctaeluciae</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;">, was estimated to number between 1200 and 1700 individuals, whereas the Martinique subspecies, <i></i></span><i><i><span style="font-family:Verdana;">Ramphocinclus</span></i><span> <i><span style="font-family:Verdana;">brachyurus</span></i> <i><span style="font-family:Verdana;">brachyurus</span></i><span style="font-family:Verdana;"></span></span></i><span style="font-family:Verdana;">, was estimated at between 200 and 400 individuals. As an endemic rare species with a high risk of extinction, the Martinique subspecies should be regarded as a conservation priority. The small size of the Martinique subspecies population, its restricted geographical range, and its narrow habitat tolerance make it quite difficult to study. The variability of morphological traits in this subspecies has not been biostatistically analyzed recently. In</span><span style="font-family:Verdana;"> order to update knowledge and fill in any gaps, we have used the morphometric approach to study the morphological variability of the Martinique subspecies. The study site is a small peninsula located in the northeast of t</span><span style="font-family:Verdana;">he island of Martinique (French West Indies) known as “la Caravelle”. We captured 63 White-breasted Thrashers which were marked, measured, and sexed before being released. We did not observe any significant differences betwe</span><span style="font-family:Verdana;">en the sexes, signifying an absence of sexual dimorphism. However, we were able to observe a significant variation in morphological traits between individuals measured inside and outside the national protected area of Ca</span><span style="font-family:Verdana;">ravelle. The body mass-tarsus ratio was equal at both sampled sites, something which could potentially be a clue to the bird’s adaptation to different habitats.</span>
文摘Streptococcus dysgalactiae subspecies equisimilis (SDSE) is a β-hemolytic Streptococcus that possesses genetic and clinical similarities to Streptococcus pyogenes. It is increasingly recognized as the etiological microorganism of invasive diseases. We report a case of a 74-year-old male who was admitted to this hospital with lower back and neck pain and infected with leg ulcer. The diagnosis of spondylodiscitis C2-C3 and L1-L3 caused by Streptococcus dysgalactiae subsp. equisimilis was made. The present case demonstrates the risk of older patients of developing invasive disease upon skin infection with Streptococcus dysgalactiae subsp. equisimilis, even when risk factors are absent or well controlled (as was diabetes mellitus in this case), suggesting that the pathogenic potential of SDSE should not be underestimated.
文摘The cultivated rice(Oryza sativa L.)is known to contain two major subspecies,indica(O. stativa L. ssp. indica)and japonica(O. sativa L. ssp.japonica). The indica and japonica differentiation
基金Supported by the Scientific Research Project from the Health Commission of Mianyang City,No.201903.
文摘BACKGROUND Streptococcus gallolyticus subspecies pasteurianus(SGSP)is a rare pathogen responsible for infant sepsis and meningitis and is potentially overlooked because it is not included in routine group B streptococcal screenings.Hence,we present a case of SGSP-induced infant meningitis and sepsis,accompanied by bronchopneumonia induced by multidrug-resistant Staphylococcus aureus(MRSA),providing insights into the identification,management,and prognosis of this bacterial infection.CASE SUMMARY A 45-day-old female infant presented with two episodes of high fever(maximum temperature:39.5°C)and two generalized grand mal seizure episodes that lasted over ten seconds and self-resolved without concomitant symptoms.Postadmission,the patient’s C-reactive protein level was 40.73 mg/L,white blood cell count was 13.42×10^(9)/L,neutrophil ratio was 78.4%,procalcitonin level was 7.89μg/L,cerebrospinal fluid(CSF)white cell count was 36×10^(6)/L,multinucleated cell ratio was 95.2%,and protein concentration was 0.41 g/L.Blood and CSF culture revealed that the pathogen was SGSP.The bacterium was sensitive to ampicillin,furazolidone,penicillin,lincomycin,moxifloxacin,rifampicin,vancomycin,and levofloxacin but resistant to clindamycin and tetracycline.Sputum culture revealed the presence of MRSA,which was sensitive to vancomycin.The patient was diagnosed with meningitis and sepsis caused by SGSP,accompanied by bronchopneumonia induced by MRSA.Ceftriaxone(100 mg/kg/d)combined with vancomycin(10 mg/kg/dose,q6h)was given as an anti-infective treatment postadmission.After 12 days of treatment,the infant was discharged from the hospital with normal CSF,blood culture,and routine blood test results,and no complications,such as subdural effusion,were observed on cranial computed tomography.No growth retardation or neurological sequelae occurred during follow-up.CONCLUSION SGPSP-induced infant bacterial meningitis and sepsis should be treated with prompt blood and CSF cultures,and a sensitive antibiotic therapy to ensure a favorable prognosis.
文摘Understanding the biological parameters of some triatomine subspecies of Meccus phyllosomus (Burmeister) is a crucial first step in estimating the epidemiologie importance of this group. Biological parameters related to hatching, lifetime, number of blood meals to molt, percentage of females at the end of the cycle, number of laid eggs, and mortality for each instar of 3 M. phyllosomus subspecies [M. p. mazzottii (Usinger), M. p. pallidipennis (Stal), and M. p. phyllosomus] and their laboratory hybrids were evaluated and compared. No significant differences (P 〉 0.05) were found among the experimental hybrids (MaPa, MaPhy, PaPhy) and reciprocal cohorts. In 5 (hatching, number of blood meals to molt, accumulative mortality, percentage of females, and mean number of laid eggs) of the 6 studied parameters (with the exception of development time), the hybrid cohorts had better fitness results than the parental cohorts involved in each set of crosses. The increase in hybrid fitness found in our study could lead to an increase in the epidemiologic risks caused by transmission of Trypanosoma cruzi to humans.
基金supported by the National Natural Science Foundation of China[grant numbers 31672306 and 32070451 to Y.H.Z.and 31872227 to J.X.Z.]grants from the Strategic Priority Research Program of the Chinese Academy of Sciences[grant number XDB11010400 to J.X.Z.].
文摘Pheromonal communication plays a key role in the sociosexual behavior of rodents.The coadaptation between pheromones and chemosensory systems has been well illustrated in insects but poorly investigated in rodents and other mammals.We aimed to investigate whether coadaptation between male pheromones and female reception might have occurred in brown rats Rattus norve-gicus.We recently reported that major urinary protein(MUP)pheromones are associated with male mating success in a brown rat subspecies,R.n.humiliatus(Rnh).Here,we discovered that MUPs were less polymorphic and occurred at much lower concentrations in males of a parapatric subspecies,R.n.caraco(Rnc),than in Rnh males,and found no association between pheromones and paternity success.Moreover,the observation of Rnc males that experienced chronic dyadic encounters and established dominance-submission relationships revealed that the dominant males achieved greater mating success than the subordinate males,but their MUP levels did not differ by social status.These findings suggest that male mating success in Rnc rats is related to social rank rather than to pheromone levels and that low concentration of MUPs might not be a reliable signal for mate choice in Rnc rats,which is different from the findings obtained in Rnh rats.In addition,compared with Rnh females,Rnc females exhibited reduced expression of pheromone receptor genes,and a lower number of vomeronasal receptor neurons were activated by MUP pheromones,which imply that the female chemosensory reception of pheromones might be structurally and functionally coadapted with male pheromone signals in brown rats.
文摘Having reviewed the major classification systems proposed by various scholars across the world, it is found that \%indica\% and \%japonica\% under \%O.sativa\% L. are two major directions thoroughly differentiated from the Asian cultivated rice, forming the framework of the classification structure. A system with \%indica\% and \%japonica\% as the only two subspecies is therefore reiterated.There are various ways to determine the indica_japonica identity of hybrid rice, but the "combined morphological trait index"(CMT index) method is more efficient and easier to handle, although the isozyme analysis, molecular marker analysis and grain quality assay methods are also feasible.
文摘Exploring the indica-japonica differentiation in parents of hybridization can provide theoretical bases for utilizing inter-subspecific heterosis. In this study, 5 sterile lines and 18 self-bred restorer lines were used as female parents and male parents respectively. Then 90 combinations were constructed by incomplete diallel cross followed by relationship analysis between parental Cheng’s index difference value and Euclidean distance and heterosis. The results showed a significant correlation between several phenotype values, super male parent heterosis and control heterosis and Euclidean distance or Cheng’s index difference value. However, it was no significant correlation for yield. Further analysis found a common interval, 3.41 - 3.46 for Euclidean distance and 3 - 4 for cheng’s index difference value of parents, which was significant or high significant positive correlated with phenotype value, super male parent and control heterosis of main yield traits. This illustrates that the larger the genetic difference of parents was, the stronger the heterosis combinations were, when the genetic differences of parents were in an appropriate range.