To determine the effect of 6 ＋ 8 and 1.5 ＋ 10 HMW-GS of synthetic hexaploid wheat （SHW） on main quality parameters of wheat, a set of recombinant inbred lines （RILs） derived from the cross between a SHW with N,...To determine the effect of 6 ＋ 8 and 1.5 ＋ 10 HMW-GS of synthetic hexaploid wheat （SHW） on main quality parameters of wheat, a set of recombinant inbred lines （RILs） derived from the cross between a SHW with N, 6 ＋ 8, 1.5 ＋ 10 HMW-GS and a cultivar Chuanyu 12-1 （CY 12-1） with 1, 7 ＋ 8, 2 ＋ 12 were planted in three environments in 2005 and 2006 and totally 16 quality parameters were tested for each line. Significant differences in all tested quality parameters but flour yield were observed between the two parents. The mean values of the RILs were intermediate to the parents for grain and protein parameters and some farinograph parameters, flour water absorption （FWA）, and farinograph softening （SOF） but beyond parents at dough stability time （DST）, breakdown time （BRT）, quality number （QN）, noodle score （NS）, and loaf volume （LOV）. All of the quality traits, especially in grain hardness （GH）, zeleny sedimentation volume （SED）, and most of farinograph parameters had significant difference between the different HMW-GS components. The effects of different alleles of HMW-GS at same locus （Glu-A1 or Glu-B1 or GIu-D1） on the different quality parameters were also different and affected by the other two loci. For most of parameters tested, 6 ＋ 8 was better than 7 ＋ 8 and there was no difference between 1.5 ＋ 10 and 2 ＋ 12. End-use quality was greatly influenced by components of HMW-GS. The components of 1, 6 ＋ 8, 1.5 ＋ 10 had the highest LOV and bread score （BS） values, whereas the components of 1, 7＋ 8 and 1.5 ＋ 10 had the highest NS values. Noodle score performed a positive linear relationship with falling number （FN） and its relationships to other quality parameters were affected by environments. Loaf volume had a significant negative relationship to SOF and positive associations with most of quality parameters. It could be concluded that HMW-GS 6＋ 8 from SHW had better overall quality characteristics than 7 ＋ 8, whereas the effects of 1.5 ＋ 10 on quality was different in respect to quality parameters and the HMW-GS components. Synthetic hexaploid wheat with subunits 6 ＋ 8 and 1.5 ＋ 10 had the potentials to improve the end-use quality of wheat cultivars.展开更多
In this work,a high-strength Mg–10Gd–6Y–1.5Zn–0.5Zr(wt.%)alloy was fabricated by successive multi-pass equal channel angular pressing(ECAP).The microstructure and mechanical property of as-cast and ECAP alloys wer...In this work,a high-strength Mg–10Gd–6Y–1.5Zn–0.5Zr(wt.%)alloy was fabricated by successive multi-pass equal channel angular pressing(ECAP).The microstructure and mechanical property of as-cast and ECAP alloys were systematically researched by X-ray diffractometer,scanning electron microscopy,transmission electron microscopy and compression test.The results show that the microstructure of as-cast alloy consists ofα-Mg grains,Mg24Y5 networks,18R blocks,fine 14H lamellas,and fewY-rich particles.After 8 passes ECAP,dynamic recrystallization ofα-Mg is developed and their average grain size decreases to about 1μm.The network Mg_(24)Y_(5) phase at grain boundaries is broken into small particles with average diameter lower than 0.5μm.Moreover,18R blocks are kinked and delaminated,or broken into small particles and blended with Mg24Y5 particles.14H lamellas grow gradually or are dynamically precipitated within certainα-Mg grains.Compression tests indicate that 8p ECAP alloy exhibits excellent mechanical property with compressive strength of 537 MPa and fracture strain of 17.0%.The significant improvement for both strength and ductility of deformed alloy could be ascribed to DRX grains,refined Mg24Y5 particles,18R kinking and dynamical precipitation of 14H.展开更多
Solid-state diffusion bonding has been done to produce transition joint between TC4 and QAl10-3-1.5 at the temperature 850℃ for 60 min. The produced diffusion bonded couples were evaluated by scanning electron micros...Solid-state diffusion bonding has been done to produce transition joint between TC4 and QAl10-3-1.5 at the temperature 850℃ for 60 min. The produced diffusion bonded couples were evaluated by scanning electron microscopy （SEM）, energy dispersive spectroscopy （EDS） and X-ray diffraction （XRD）. SEM revealed the presences of the different reaction regions in the diffusion zone and “kirkendall voids” were observed in the interface. This study indicated the existences of Cu3 Ti, Cu and Ti phases in the diffusion zone of the joint confirmed by XRD. The microhardness tests revealed that the microhardness of TC4 jointing affected zone （ JAZ） is higher than the TC4 base materials, and transition zone close to TC4 possesses the highest microhardness value.展开更多
BACKGROUND Interleukin 10 receptor alpha subunit(IL10RA)dysfunction is the main cause of very early-onset inflammatory bowel disease(VEO-IBD)in East Asians.AIM To identify disease-causing gene mutations in four patien...BACKGROUND Interleukin 10 receptor alpha subunit(IL10RA)dysfunction is the main cause of very early-onset inflammatory bowel disease(VEO-IBD)in East Asians.AIM To identify disease-causing gene mutations in four patients with VEO-IBD and verify functional changes related to the disease-causing mutations.METHODS From May 2016 to September 2020,four young patients with clinically diagnosed VEO-IBD were recruited.Before hospitalization,using targeted gene panel sequencing and trio-whole-exome sequencing(WES),three patients were found to harbor a IL10RA mutation(c.301C>T,p.R101W in one patient;c.537G>A,p.T179T in two patients),but WES results of the fourth patient were not conclusive.We performed whole-genome sequencing(WGS)on patients A and B and reanalyzed the data from patients C and D.Peripheral blood mononuclear cells(PBMCs)from patient D were isolated and stimulated with lipopolysaccharide(LPS),interleukin 10(IL-10),and LPS+IL-10.Serum IL-10 levels in four patients and tumor necrosis factor-α(TNF-α)in the cell supernatant were determined by enzyme-linked immunosorbent assay.Phosphorylation of signal transducer and activator of transcription 3(STAT3)at Tyr705 and Ser727 in PBMCs was determined by western blot analysis.RESULTS The four children in our study consisted of two males and two females.The age at disease onset ranged from 18 d to 9 mo.After hospitalization,a novel 333-bp deletion encompassing exon 1 of IL10RA was found in patients A and B using WGS and was found in patients C and D after reanalysis of their WES data.Patient D was homozygous for the 333 bp deletion.All four patients had elevated serum IL-10 levels.In vitro,IL-10-stimulated PBMCs from patient D failed to induce STAT3 phosphorylation at Tyr705 and only minimally suppressed TNF-αproduction induced by LPS.Phosphorylation at Ser727 in PBMCs was not affected by LPS or LPS+IL-10 in both healthy subjects and in patient D.CONCLUSION WGS revealed a novel 333-bp deletion of IL10RA in four patients with VEO-IBD,whereas the WES results were inconclusive.展开更多
基金the Sichuan Provincial Youth Foundation (04ZQ026-009)National 863 Pro-gram of China (2006AA10Z1C6)+1 种基金National Natural Science Foundation of China (30771338)Na-tional Key Technology R&D Program of China(2006BAD01A02, 2006BAD13B02)
文摘To determine the effect of 6 ＋ 8 and 1.5 ＋ 10 HMW-GS of synthetic hexaploid wheat （SHW） on main quality parameters of wheat, a set of recombinant inbred lines （RILs） derived from the cross between a SHW with N, 6 ＋ 8, 1.5 ＋ 10 HMW-GS and a cultivar Chuanyu 12-1 （CY 12-1） with 1, 7 ＋ 8, 2 ＋ 12 were planted in three environments in 2005 and 2006 and totally 16 quality parameters were tested for each line. Significant differences in all tested quality parameters but flour yield were observed between the two parents. The mean values of the RILs were intermediate to the parents for grain and protein parameters and some farinograph parameters, flour water absorption （FWA）, and farinograph softening （SOF） but beyond parents at dough stability time （DST）, breakdown time （BRT）, quality number （QN）, noodle score （NS）, and loaf volume （LOV）. All of the quality traits, especially in grain hardness （GH）, zeleny sedimentation volume （SED）, and most of farinograph parameters had significant difference between the different HMW-GS components. The effects of different alleles of HMW-GS at same locus （Glu-A1 or Glu-B1 or GIu-D1） on the different quality parameters were also different and affected by the other two loci. For most of parameters tested, 6 ＋ 8 was better than 7 ＋ 8 and there was no difference between 1.5 ＋ 10 and 2 ＋ 12. End-use quality was greatly influenced by components of HMW-GS. The components of 1, 6 ＋ 8, 1.5 ＋ 10 had the highest LOV and bread score （BS） values, whereas the components of 1, 7＋ 8 and 1.5 ＋ 10 had the highest NS values. Noodle score performed a positive linear relationship with falling number （FN） and its relationships to other quality parameters were affected by environments. Loaf volume had a significant negative relationship to SOF and positive associations with most of quality parameters. It could be concluded that HMW-GS 6＋ 8 from SHW had better overall quality characteristics than 7 ＋ 8, whereas the effects of 1.5 ＋ 10 on quality was different in respect to quality parameters and the HMW-GS components. Synthetic hexaploid wheat with subunits 6 ＋ 8 and 1.5 ＋ 10 had the potentials to improve the end-use quality of wheat cultivars.
基金the Natural Science Foundation of Jiangsu Province of China(No.BK20160869)the financial support from the Nantong Science and Technology Project(No.GY12015009)+1 种基金the Fundamental Research Funds for the Central Universities(No.2015B01314)the National Natural Science Foundation of China(No.51501039).
文摘In this work,a high-strength Mg–10Gd–6Y–1.5Zn–0.5Zr(wt.%)alloy was fabricated by successive multi-pass equal channel angular pressing(ECAP).The microstructure and mechanical property of as-cast and ECAP alloys were systematically researched by X-ray diffractometer,scanning electron microscopy,transmission electron microscopy and compression test.The results show that the microstructure of as-cast alloy consists ofα-Mg grains,Mg24Y5 networks,18R blocks,fine 14H lamellas,and fewY-rich particles.After 8 passes ECAP,dynamic recrystallization ofα-Mg is developed and their average grain size decreases to about 1μm.The network Mg_(24)Y_(5) phase at grain boundaries is broken into small particles with average diameter lower than 0.5μm.Moreover,18R blocks are kinked and delaminated,or broken into small particles and blended with Mg24Y5 particles.14H lamellas grow gradually or are dynamically precipitated within certainα-Mg grains.Compression tests indicate that 8p ECAP alloy exhibits excellent mechanical property with compressive strength of 537 MPa and fracture strain of 17.0%.The significant improvement for both strength and ductility of deformed alloy could be ascribed to DRX grains,refined Mg24Y5 particles,18R kinking and dynamical precipitation of 14H.
文摘Solid-state diffusion bonding has been done to produce transition joint between TC4 and QAl10-3-1.5 at the temperature 850℃ for 60 min. The produced diffusion bonded couples were evaluated by scanning electron microscopy （SEM）, energy dispersive spectroscopy （EDS） and X-ray diffraction （XRD）. SEM revealed the presences of the different reaction regions in the diffusion zone and “kirkendall voids” were observed in the interface. This study indicated the existences of Cu3 Ti, Cu and Ti phases in the diffusion zone of the joint confirmed by XRD. The microhardness tests revealed that the microhardness of TC4 jointing affected zone （ JAZ） is higher than the TC4 base materials, and transition zone close to TC4 possesses the highest microhardness value.
基金the National Natural Science Foundation of China,No.81741103.
文摘BACKGROUND Interleukin 10 receptor alpha subunit(IL10RA)dysfunction is the main cause of very early-onset inflammatory bowel disease(VEO-IBD)in East Asians.AIM To identify disease-causing gene mutations in four patients with VEO-IBD and verify functional changes related to the disease-causing mutations.METHODS From May 2016 to September 2020,four young patients with clinically diagnosed VEO-IBD were recruited.Before hospitalization,using targeted gene panel sequencing and trio-whole-exome sequencing(WES),three patients were found to harbor a IL10RA mutation(c.301C>T,p.R101W in one patient;c.537G>A,p.T179T in two patients),but WES results of the fourth patient were not conclusive.We performed whole-genome sequencing(WGS)on patients A and B and reanalyzed the data from patients C and D.Peripheral blood mononuclear cells(PBMCs)from patient D were isolated and stimulated with lipopolysaccharide(LPS),interleukin 10(IL-10),and LPS+IL-10.Serum IL-10 levels in four patients and tumor necrosis factor-α(TNF-α)in the cell supernatant were determined by enzyme-linked immunosorbent assay.Phosphorylation of signal transducer and activator of transcription 3(STAT3)at Tyr705 and Ser727 in PBMCs was determined by western blot analysis.RESULTS The four children in our study consisted of two males and two females.The age at disease onset ranged from 18 d to 9 mo.After hospitalization,a novel 333-bp deletion encompassing exon 1 of IL10RA was found in patients A and B using WGS and was found in patients C and D after reanalysis of their WES data.Patient D was homozygous for the 333 bp deletion.All four patients had elevated serum IL-10 levels.In vitro,IL-10-stimulated PBMCs from patient D failed to induce STAT3 phosphorylation at Tyr705 and only minimally suppressed TNF-αproduction induced by LPS.Phosphorylation at Ser727 in PBMCs was not affected by LPS or LPS+IL-10 in both healthy subjects and in patient D.CONCLUSION WGS revealed a novel 333-bp deletion of IL10RA in four patients with VEO-IBD,whereas the WES results were inconclusive.
