[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as m...[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as materials for selecting the primary medium, subculture medium, rooting medium and acclimatization substrate during the tissue culture and rapid propagation. [Result] 6-BA was the optimal cytokinin for tissue culture of Lonicera edulis Turcz, compared with ZT; modified MS+1.0 mg/L of 6-BA + 0.2 mg/L of IBA was the optimal medium as primary and subculture medium, modified MS+ 1.5 mg/L of IBA was the optimal medium for rooting of Lonicera edulis Turcz, the rooting rate had achieved 100% after cultured for 30 d. The optimal substrate for transplanting plantlets of Lonicera edulis Turcz was composed of humus and perlite (1∶ 1, V/V), survival rate was as high as 95% after 30 d. [Conclusion] This study provided basis for the rapid propagation of superior seedlings of Lonicera edulis Turcz, as well as the establishment of industrialized breeding technical system and the implementation of scale production.展开更多
One of the outstanding differences between Eastern and Western cultures is the difference in the perception of individualism and collectivism. For Westerners, everything serves the individual's values and rights, whi...One of the outstanding differences between Eastern and Western cultures is the difference in the perception of individualism and collectivism. For Westerners, everything serves the individual's values and rights, while Chinese believe the collective is superior to everything as there cannot be a self without a collective.展开更多
基金Supported by the National Natural Science Fundation of China(30960231)Project of the State Forestry Administration(2005-06)~~
文摘[Objective] This paper aimed to study the tissue culture and rapid propagation technology of superior individuals of Lonicera edulis Turcz. [Method] Several superior individuals of Lonicera edulis Turcz were used as materials for selecting the primary medium, subculture medium, rooting medium and acclimatization substrate during the tissue culture and rapid propagation. [Result] 6-BA was the optimal cytokinin for tissue culture of Lonicera edulis Turcz, compared with ZT; modified MS+1.0 mg/L of 6-BA + 0.2 mg/L of IBA was the optimal medium as primary and subculture medium, modified MS+ 1.5 mg/L of IBA was the optimal medium for rooting of Lonicera edulis Turcz, the rooting rate had achieved 100% after cultured for 30 d. The optimal substrate for transplanting plantlets of Lonicera edulis Turcz was composed of humus and perlite (1∶ 1, V/V), survival rate was as high as 95% after 30 d. [Conclusion] This study provided basis for the rapid propagation of superior seedlings of Lonicera edulis Turcz, as well as the establishment of industrialized breeding technical system and the implementation of scale production.
文摘One of the outstanding differences between Eastern and Western cultures is the difference in the perception of individualism and collectivism. For Westerners, everything serves the individual's values and rights, while Chinese believe the collective is superior to everything as there cannot be a self without a collective.