Amyotrophic lateral sclerosis(ALS)is a neuromuscular condition resulting from the progressive degeneration of motor neurons in the cortex,brainstem,and spinal cord.While the typical clinical phenotype of ALS involves ...Amyotrophic lateral sclerosis(ALS)is a neuromuscular condition resulting from the progressive degeneration of motor neurons in the cortex,brainstem,and spinal cord.While the typical clinical phenotype of ALS involves both upper and lower motor neurons,human and animal studies over the years have highlighted the potential spread to other motor and non-motor regions,expanding the phenotype of ALS.Although superoxide dismutase 1(SOD1)mutations represent a minority of ALS cases,the SOD1 gene remains a milestone in ALS research as it represents the first genetic target for personalized therapies.Despite numerous single case reports or case series exhibiting extramotor symptoms in patients with ALS mutations in SOD1(SOD1-ALS),no studies have comprehensively explored the full spectrum of extramotor neurological manifestations in this subpopulation.In this narrative review,we analyze and discuss the available literature on extrapyramidal and non-motor features during SOD1-ALS.The multifaceted expression of SOD1 could deepen our understanding of the pathogenic mechanisms,pointing towards a multidisciplinary approach for affected patients in light of new therapeutic strategies for SOD1-ALS.展开更多
Though bacteria of the radiation-resistant Deinococcus radiodurans have a high resistance to the lethal and mutagenic effects of many DNA-damaging agents, the mechanisms involved in the response of these bacteria to o...Though bacteria of the radiation-resistant Deinococcus radiodurans have a high resistance to the lethal and mutagenic effects of many DNA-damaging agents, the mechanisms involved in the response of these bacteria to oxidative stress are poorly understood. In this report, the superoxide dismutase (SOD) and catalase (CAT) activities produced by these bacteria were measured, and the change of SOD and CAT activities by 20 keV N+ beam exposure was examined. Their activities were increased by N+ beam exposure from 8x 1014 ions/cm2 to 6x1015 ions/cm2.The treatment of H2O2 and [ CHC13 +CH3 CH2OH ] and the measurement of absorption spectrum showed that the increase in SOD activity was resulted from inducible activities of MnSOD in D. radiodurans AS1.633 by N+ beam exposure . These results suggested that this bacteria possess inducible defense mechanisms against the deleterious effects of oxidization.展开更多
Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their abili...Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their ability to reduce the damaging properties of reactive oxygen species within cells and organism.This review discusses the necessary requirements for nanoparticles to have SOD activity and reveals a close relationship between catalysis on prebiotic earth and the recent SOD mimics.This review also aims to highlight the progress in the development of SOD mimicking nanoparticles.We give a broad overview of nanoparticles with SOD activity,based on their material make-up,to underline their increasing diversity.展开更多
The 3rd Hospital of Wuchang District ,Wuhan 432600)The squamous cell carcinoma of lung was induced with methylcholanthrene (MCA) in iodized oil in wistar rts- During the development of the cancer, the animals were giv...The 3rd Hospital of Wuchang District ,Wuhan 432600)The squamous cell carcinoma of lung was induced with methylcholanthrene (MCA) in iodized oil in wistar rts- During the development of the cancer, the animals were given superoxide dismutase (SOD) or its inhibitor diethyldio-carhamate (DDC). In DDC group, 3 out of 50 rats developed cancer and 4 developed atypical hyperplasia of hronchial epithelium within 35 to 40 days.In SOD group, no cancer developed in all of the 52 rats.and only one had atypical hyperplasia in the lungs. Only one of 42 control rats had cancer and 2 rats had atypical hyperplasia of bronchial epithelium . The difference in cancer frequency between groups DDC and SOD was significant (P<0.05). The results suggest that there is a synergism between DDC and MCA in the induction of lung cancer, while SOD can inhibit MCA-induced lung cancer development. The mechanism of the effect of SOD and DDC was discussed.展开更多
Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion...Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion of erythrocytes in 18 patients with dilated cardiomyopathy and 16 healthy controls were measured. Results Superoxide dlsmutase(SOD) activites of erythrocytes were lower in dilated cardiomyopathy(DCM) patients than that in healthy controls (P <o. oo1 ). The lipids composition of erythrocytes has changed in the DCM patients compared with healthy controls: total liplds changed little (P >o. o5); total phospholipids were lower, but not significantly (P>o. o5): total cholesterol increased significantly (P <o. o5). The cholesterol to phospholipids molecular ratio of erythrocyte membrane has increased remarkably (P >o. o5). Conclusion It can be supposed that decreased SOD activitles play an important role in the damage or membrane system and the pathogensis of DCM.展开更多
The dismutation kinetics of superoxide anion by copper complex of 1,4,7,10-tetranza- cyctotridecane-11,13-dione was studied by pulse radiolysis.The dismutation rate constants at various pH and concentration of complex...The dismutation kinetics of superoxide anion by copper complex of 1,4,7,10-tetranza- cyctotridecane-11,13-dione was studied by pulse radiolysis.The dismutation rate constants at various pH and concentration of complex were obtained.展开更多
目的探讨通心络胶囊对进展型缺血性脑卒中患者血清糖基化终产物(advanced glycation end products,AGE)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、总抗氧化能力(Total antioxidant capacity,T-AOC)水...目的探讨通心络胶囊对进展型缺血性脑卒中患者血清糖基化终产物(advanced glycation end products,AGE)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、总抗氧化能力(Total antioxidant capacity,T-AOC)水平的影响。方法选取诊治的进展型缺血性脑卒中患者60例,随机分为对照组与实验组,每组30例,对照组进行常规综合治疗,实验组在常规综合治疗基础上给予通心络胶囊口服治疗,两组均治疗14 d,观察治疗前后血清AGE、MDA、SOD、T-AOC水平、神经功能缺损严重程度,并对治疗疗效进行评估。结果两组治疗前血清AGE、MDA、SOD、T-AOC水平比较差异无统计学意义(P>0.05),AGE、MDA后较治疗前均下降(P<0.05),且实验组下降幅度大于对照组(P<0.05),SOD、T-AOC治疗后较治疗前升高(P<0.05),且实验组升高幅度大于对照组(P<0.05);两组治疗前美国国立卫生院卒中量表(National Institutes of Health Stroke Scale,NIHSS)及格拉斯哥昏迷指数(Glasgow Coma Scale,GCS)评分比较差异无统计学意义(P>0.05),NIHSS评分治疗后较治疗前均下降(P<0.05),且实验组下降幅度大于对照组(P<0.05),GCS评分治疗后较治疗前升高(P<0.05),且实验组升高幅度大于对照组(P<0.05);对照组与实验组的治疗总有效率分别为73.33%(22/30)、93.33%(28/30),比较差异有统计学意义(P<0.05);Sperman相关性分析显示,进展型缺血性脑卒中患者NIHSS评分与血清AGE和MDA水平呈显著正相关(P<0.05),与SOD和T-AOC水平呈显著负相关(P<0.05),患者GCS评分与血清AGE和MDA水平呈显著负相关(P<0.05),与SOD和T-AOC水平呈显著正相关(P<0.05)。结论进展型缺血性脑卒中患者血清AGE、MDA、SOD、T-AOC水平与神经功能缺损密切相关,采用通心络胶囊治疗可有效改善患者血清AGE、MDA、SOD、T-AOC水平,促进患者神经功能的改善,提高疗效。展开更多
Rational design of metallic active sites and its microenvironment is critical for constructing superoxide dismutase(SOD)nanozymes.Here,we reported a novel SOD nanozyme design,with employing graphene oxide(GO)as the fr...Rational design of metallic active sites and its microenvironment is critical for constructing superoxide dismutase(SOD)nanozymes.Here,we reported a novel SOD nanozyme design,with employing graphene oxide(GO)as the framework,andδ-MnO_(2)as the active sites,to mimic the natural Mn-SOD.This MnO_(2)@GO nanozyme exhibited multiscale laminated structures with honeycomb-like morphology,providing highly specific surface area for·O_(2)−adsorption and confined spaces for subsequent catalytic reactions.Thus,the nanozyme achieved superlative SOD-like catalytic performance with inhibition rate of 95.5%,which is 222.6%and 1605.4%amplification over GO and MnO_(2)nanoparticles,respectively.Additionally,such unique hierarchical structural design endows MnO_(2)@GO with catalytic specificity,which was not present in the individual component(GO or MnO_(2)).This multiscale structural design provides new strategies for developing highly active and specific SOD nanozymes.展开更多
文摘Amyotrophic lateral sclerosis(ALS)is a neuromuscular condition resulting from the progressive degeneration of motor neurons in the cortex,brainstem,and spinal cord.While the typical clinical phenotype of ALS involves both upper and lower motor neurons,human and animal studies over the years have highlighted the potential spread to other motor and non-motor regions,expanding the phenotype of ALS.Although superoxide dismutase 1(SOD1)mutations represent a minority of ALS cases,the SOD1 gene remains a milestone in ALS research as it represents the first genetic target for personalized therapies.Despite numerous single case reports or case series exhibiting extramotor symptoms in patients with ALS mutations in SOD1(SOD1-ALS),no studies have comprehensively explored the full spectrum of extramotor neurological manifestations in this subpopulation.In this narrative review,we analyze and discuss the available literature on extrapyramidal and non-motor features during SOD1-ALS.The multifaceted expression of SOD1 could deepen our understanding of the pathogenic mechanisms,pointing towards a multidisciplinary approach for affected patients in light of new therapeutic strategies for SOD1-ALS.
基金This work was supported by the National Nature Science Foundation of China! No.19605005
文摘Though bacteria of the radiation-resistant Deinococcus radiodurans have a high resistance to the lethal and mutagenic effects of many DNA-damaging agents, the mechanisms involved in the response of these bacteria to oxidative stress are poorly understood. In this report, the superoxide dismutase (SOD) and catalase (CAT) activities produced by these bacteria were measured, and the change of SOD and CAT activities by 20 keV N+ beam exposure was examined. Their activities were increased by N+ beam exposure from 8x 1014 ions/cm2 to 6x1015 ions/cm2.The treatment of H2O2 and [ CHC13 +CH3 CH2OH ] and the measurement of absorption spectrum showed that the increase in SOD activity was resulted from inducible activities of MnSOD in D. radiodurans AS1.633 by N+ beam exposure . These results suggested that this bacteria possess inducible defense mechanisms against the deleterious effects of oxidization.
文摘Inorganic solids with enzyme-like activity are promising to overcome many restrictions of native enzymes in application.Especially attractive are nanoparticles with superoxide dismutase(SOD)activity,due to their ability to reduce the damaging properties of reactive oxygen species within cells and organism.This review discusses the necessary requirements for nanoparticles to have SOD activity and reveals a close relationship between catalysis on prebiotic earth and the recent SOD mimics.This review also aims to highlight the progress in the development of SOD mimicking nanoparticles.We give a broad overview of nanoparticles with SOD activity,based on their material make-up,to underline their increasing diversity.
文摘The 3rd Hospital of Wuchang District ,Wuhan 432600)The squamous cell carcinoma of lung was induced with methylcholanthrene (MCA) in iodized oil in wistar rts- During the development of the cancer, the animals were given superoxide dismutase (SOD) or its inhibitor diethyldio-carhamate (DDC). In DDC group, 3 out of 50 rats developed cancer and 4 developed atypical hyperplasia of hronchial epithelium within 35 to 40 days.In SOD group, no cancer developed in all of the 52 rats.and only one had atypical hyperplasia in the lungs. Only one of 42 control rats had cancer and 2 rats had atypical hyperplasia of bronchial epithelium . The difference in cancer frequency between groups DDC and SOD was significant (P<0.05). The results suggest that there is a synergism between DDC and MCA in the induction of lung cancer, while SOD can inhibit MCA-induced lung cancer development. The mechanism of the effect of SOD and DDC was discussed.
文摘Objective To rurther conflrm the role or lipld-peroxldation caused by oxygen free radicals injury played in the pathogenesis of dllsted cardlomypathy. MethOds The superoxide dismutase activities and lipids composltion of erythrocytes in 18 patients with dilated cardiomyopathy and 16 healthy controls were measured. Results Superoxide dlsmutase(SOD) activites of erythrocytes were lower in dilated cardiomyopathy(DCM) patients than that in healthy controls (P <o. oo1 ). The lipids composition of erythrocytes has changed in the DCM patients compared with healthy controls: total liplds changed little (P >o. o5); total phospholipids were lower, but not significantly (P>o. o5): total cholesterol increased significantly (P <o. o5). The cholesterol to phospholipids molecular ratio of erythrocyte membrane has increased remarkably (P >o. o5). Conclusion It can be supposed that decreased SOD activitles play an important role in the damage or membrane system and the pathogensis of DCM.
文摘The dismutation kinetics of superoxide anion by copper complex of 1,4,7,10-tetranza- cyctotridecane-11,13-dione was studied by pulse radiolysis.The dismutation rate constants at various pH and concentration of complex were obtained.
文摘目的探讨通心络胶囊对进展型缺血性脑卒中患者血清糖基化终产物(advanced glycation end products,AGE)、丙二醛(malondialdehyde,MDA)、超氧化物歧化酶(superoxide dismutase,SOD)、总抗氧化能力(Total antioxidant capacity,T-AOC)水平的影响。方法选取诊治的进展型缺血性脑卒中患者60例,随机分为对照组与实验组,每组30例,对照组进行常规综合治疗,实验组在常规综合治疗基础上给予通心络胶囊口服治疗,两组均治疗14 d,观察治疗前后血清AGE、MDA、SOD、T-AOC水平、神经功能缺损严重程度,并对治疗疗效进行评估。结果两组治疗前血清AGE、MDA、SOD、T-AOC水平比较差异无统计学意义(P>0.05),AGE、MDA后较治疗前均下降(P<0.05),且实验组下降幅度大于对照组(P<0.05),SOD、T-AOC治疗后较治疗前升高(P<0.05),且实验组升高幅度大于对照组(P<0.05);两组治疗前美国国立卫生院卒中量表(National Institutes of Health Stroke Scale,NIHSS)及格拉斯哥昏迷指数(Glasgow Coma Scale,GCS)评分比较差异无统计学意义(P>0.05),NIHSS评分治疗后较治疗前均下降(P<0.05),且实验组下降幅度大于对照组(P<0.05),GCS评分治疗后较治疗前升高(P<0.05),且实验组升高幅度大于对照组(P<0.05);对照组与实验组的治疗总有效率分别为73.33%(22/30)、93.33%(28/30),比较差异有统计学意义(P<0.05);Sperman相关性分析显示,进展型缺血性脑卒中患者NIHSS评分与血清AGE和MDA水平呈显著正相关(P<0.05),与SOD和T-AOC水平呈显著负相关(P<0.05),患者GCS评分与血清AGE和MDA水平呈显著负相关(P<0.05),与SOD和T-AOC水平呈显著正相关(P<0.05)。结论进展型缺血性脑卒中患者血清AGE、MDA、SOD、T-AOC水平与神经功能缺损密切相关,采用通心络胶囊治疗可有效改善患者血清AGE、MDA、SOD、T-AOC水平,促进患者神经功能的改善,提高疗效。
基金supported by the National Natural Science Foundation of China(Nos.52202344,T2225026,82172087,and 82071308)the National Key R&D Program of China(No.2022YFA1205801)Beijing Institute of Technology Research Fund Program for Young Scholars.
文摘Rational design of metallic active sites and its microenvironment is critical for constructing superoxide dismutase(SOD)nanozymes.Here,we reported a novel SOD nanozyme design,with employing graphene oxide(GO)as the framework,andδ-MnO_(2)as the active sites,to mimic the natural Mn-SOD.This MnO_(2)@GO nanozyme exhibited multiscale laminated structures with honeycomb-like morphology,providing highly specific surface area for·O_(2)−adsorption and confined spaces for subsequent catalytic reactions.Thus,the nanozyme achieved superlative SOD-like catalytic performance with inhibition rate of 95.5%,which is 222.6%and 1605.4%amplification over GO and MnO_(2)nanoparticles,respectively.Additionally,such unique hierarchical structural design endows MnO_(2)@GO with catalytic specificity,which was not present in the individual component(GO or MnO_(2)).This multiscale structural design provides new strategies for developing highly active and specific SOD nanozymes.
