Superparamagnetic iron oxide nanoparticles(SPIONs)have immeasurable potentials in many fields such as nanobiotechnology and biomedical engineering because of their superparamagnetic properties and small particle size....Superparamagnetic iron oxide nanoparticles(SPIONs)have immeasurable potentials in many fields such as nanobiotechnology and biomedical engineering because of their superparamagnetic properties and small particle size.This review introduces the methods for SPIONs synthesis,including co-precipitation,thermal decomposition,microemulsion and hydrothermal reaction,and surface modification of SPIONs with organometallic and inorganic metals,surface modification for targeted drug delivery,and the use of SPIONs as a contrast agent.In addition,this article also provides an overview of recent progress in SPIONs for the treatment of glioma,lung cancer and breast cancer.展开更多
Five types of superparamagnetic iron oxide (SPIO),i.e. Ferumoxides (Feridex? Ⅳ, Berlex Laboratories),Fe r u c a r b o t ra n ( Re s ov i s t?, B aye r H e a l t h c a re ) ,Ferumoxtran-10 (AMI-227 or Code-72...Five types of superparamagnetic iron oxide (SPIO),i.e. Ferumoxides (Feridex? Ⅳ, Berlex Laboratories),Fe r u c a r b o t ra n ( Re s ov i s t?, B aye r H e a l t h c a re ) ,Ferumoxtran-10 (AMI-227 or Code-7227, Combidex?, AMAG Pharma; Sinerem?, Guerbet), NC100150(Clariscan?, Nycomed,) and (VSOP C184, Ferropharm)have been designed and clinically tested as magneticresonance contrast agents. However, until nowResovist? is current available in only a few countries.The other four agents have been stopped for furtherdevelopment or withdrawn from the market. AnotherSPIO agent Ferumoxytol (Feraheme) is approved forthe treatment of iron deficiency in adult chronic kidneydisease patients. Ferumoxytol is comprised of ironoxide particles surrounded by a carbohydrate coat, andit is being explored as a potential imaging approach forevaluating lymph nodes and certain liver tumors.展开更多
Biodistribution and toxicity assessment are critical for safe clinical use of newly developed medicines.Superparamagnetic iron oxide nanoparticles (SPION)are effective carriers for targeted drug delivery.This study ai...Biodistribution and toxicity assessment are critical for safe clinical use of newly developed medicines.Superparamagnetic iron oxide nanoparticles (SPION)are effective carriers for targeted drug delivery.This study aimed to examine the toxicity and biodistribution of SPION coated with polyethylenimine (PEI)(SPION-PEI)designed for small interfering RNA (siRNA) delivery both in vitro and in vivo.SPION-PEI/siRNA complexes were prepared at different weight ratios.Cytotoxic effects of SPION-PEI/siRNA on HSC-T6 cell viability were determined by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT).Rats were divided into three groups:a control group,a normal-saline group and a SPION-PEI/siRNA group.After a single intravenous injection,in vivo nanoparticle biodistribution and accumulation were evaluated by Prussian blue staining in the heart,liver,spleen,lung and kidney 8 h,24 h,and 7 days after the injection.Their distribution was histologically studied at the three time points by measuring ironpositive areas (μm2)in organ sections stained with Prussian blue.The same organs were analyzed by H&E staining for any possible histopathological changes.Furthermore,biochemical indexes such as alanine amino transaminase (ALT),aspartate transaminase (AST),blood urea nitrogen (BUN)and creatinine (CREA)were also assessed at all experimental time points.Electrophoresis exhibited that the SPION-PEI could retard siRNA altogether at weight ratios above 4.MTT assay showed that SPION-PEI loaded with siRNA had low cytotoxicity.In vivo study revealed that the liver and spleen were the major sites of SPION-PEI/siRNA deposition.The iron content was significantly increased in the liver and spleen,peaking 24 h after intravenous injection and then declining gradually.No evidence was found of irreversible histopathological damage to any of the organs tested.These results suggested that most SPION-PEI/siRNA complexes were distributed in the liver and spleen,which might be the target organs of SPION-PEI/siRNA complexes.SPION- PEI/siRNA may serve as in vivo carrier for biomedical medicines.展开更多
Superparamagnetic iron oxide nanoparticles(SPIONs) are one of the most versatile and safe nanoparticles in a wide variety of biomedical applications. In the past decades, considerable efforts have been made to investi...Superparamagnetic iron oxide nanoparticles(SPIONs) are one of the most versatile and safe nanoparticles in a wide variety of biomedical applications. In the past decades, considerable efforts have been made to investigate the potential adverse biological effects and safety issues associated with SPIONs, which is essential for the development of next-generation SPIONs and for continued progress in translational research. In this mini review, we summarize recent developments in toxicity studies on SPIONs, focusing on the relationship between the physicochemical properties of SPIONs and their induced toxic biological responses for a better toxicological understanding of SPIONs.展开更多
To assess a novel cell manipulation technique of tissue engineering with respect to its ability to augment superparamagnetic iron oxide particles (SPIO) labeled mesenchymal stem cells (MSCs) density at a localized car...To assess a novel cell manipulation technique of tissue engineering with respect to its ability to augment superparamagnetic iron oxide particles (SPIO) labeled mesenchymal stem cells (MSCs) density at a localized cartilage defect site in an in vitro phantom by applying magnetic force. Meanwhile, non-invasive imaging techniques were use to track SPIO-labeled MSCs by magnetic resonance imaging (MRI). Human bone marrow MSCs were cultured and labeled with SPIO. Fresh degenerated human osteochondral fragments were obtained during total knee arthroplasty and a cartilage defect was created at the center. Then, the osteochondral fragments were attached to the sidewalls of culture flasks filled with phosphate-buffered saline (PBS) to mimic the human joint cavity. The SPIO-labeled MSCs were injected into the culture flasks in the presence of a 0.57 Tesla (T) magnetic force. Before and 90 min after cell targeting, the specimens underwent T2-weighted turbo spin-echo (SET2WI) sequence of 3.0 T MRI. MRI results were compared with histological findings. Macroscopic observation showed that SPIO-labeled MSCs were steered to the target region of cartilage defect. MRI revealed significant changes in signal intensity (P<0.01). HE staining exibited that a great number of MSCs formed a three-dimensional (3D) cell "sheet" structure at the chondral defect site. It was concluded that 0.57 T magnetic force permits spatial delivery of magnetically labeled MSCs to the target region in vitro. High-field MRI can serve as an very sensitive non-invasive technique for the visualization of SPIO-labeled MSCs.展开更多
Due to their very small size,nanoparticles can interact with all cells in the central nervous system.One of the most promising nanoparticle subgroups are very small superparamagnetic iron oxide nanoparticles(VSOP)that...Due to their very small size,nanoparticles can interact with all cells in the central nervous system.One of the most promising nanoparticle subgroups are very small superparamagnetic iron oxide nanoparticles(VSOP)that are citrate coated for electrostatic stabilization.To determine their influence on murine blood-derived monocytes,which easily enter the injured central nervous system,we applied VSOP and carboxydextran-coated superparamagnetic iron oxide nanoparticles(Resovist).We assessed their impact on the viability,cytokine,and chemokine secretion,as well as iron uptake of murine blood-derived monocytes.We found that(1)the monocytes accumulated VSOP and Resovist,(2)this uptake seemed to be nanoparticle-and time-dependent,(3)the decrease of monocytes viability was treatment-related,(4)VSOP and Resovist incubation did not alter cytokine homeostasis,and(5)overall a 6-hour treatment with 0.75 mM VSOP-R1 was probably sufficient to effectively label monocytes for future experiments.Since homeostasis is not altered,it is safe to label blood-derived monocles with VSOP.VSOP labeled monocytes can be used to study injured central nervous system sites further,for example with drug-carrying VSOP.展开更多
Background: The aim of this study was to investigate the distribution of the c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles in the major organs of mice by MR imaging. Methods: Sixty BA...Background: The aim of this study was to investigate the distribution of the c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles in the major organs of mice by MR imaging. Methods: Sixty BALB/c mice were randomly divided into experimental and control groups. MR scans were performed in each mouse of the experimental group at five different time points (10, 30, 60, 180 and 360 min) after injection of the antisense probe. The signal from each major organ (liver, spleen, heart, kidney and muscle tissue) in comparison with the background signal (signal to noise ratio) was determined at each time point as a measure of the distribution of the antisense probe. Six control mice were killed at each of the same time points and the organs immediately removed for determination of their iron content. Results: After injection of the antisense probe, the highest enrichment of the probe was seen in the spleen, reaching a peak at 180 min, followed by the liver, muscle, heart and kidney. Conclusions: MR imaging can visualize the distribution of c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles in the major organs of mice, and this may provide the basis for further in vivo studies of MR imaging time and dose selection.展开更多
Adipose-derived stem cells(ASCs) induce therapeutic angiogenesis due to pro-angiogenic cytokines secretion. Superparamagnetic iron oxide(SPIO) nanoparticles are critical for magnetic resonance(MR) tracking of implante...Adipose-derived stem cells(ASCs) induce therapeutic angiogenesis due to pro-angiogenic cytokines secretion. Superparamagnetic iron oxide(SPIO) nanoparticles are critical for magnetic resonance(MR) tracking of implanted cells. Hypoxia is a powerful stimulus for angiogenic activity of ASCs. In this study, we investigated whether therapeutic potency could be enhanced by implantation of hypoxia-preconditioned SPIO-labeled ASCs(SPIOASCs) into the infarcted myocardium. ASCs and SPIOASCs were cultured under 2% O_2(hypoxia) or 95% air(normoxia). Cells were intramyocardially injected into the infarcted myocardium after 48-h culture. We found that hypoxia culture increased the m RNA expression of hypoxia-inducible factor-1 alpha(HIF-1α) and vascular endothelial growth factor(VEGF) in ASCs and SPIOASCs. The VEGF protein in the conditioned medium was significantly higher in hypoxic ASCs and SPIOASCs than in normoxic ASCs and SPIOASCs. The capillary density and left ventricular contractile function in the infarcted myocardium were significantly higher 4 weeks after implantation with hypoxic ASCs and SPIOASCs than with normoxic ASCs and SPIOASCs. Improvement in the capillary density and left ventricle function didn't differ between hypoxic ASCs-transplanted rats and hypoxic SPIOASCs-transplanted rats. Hypoxic culture enhanced the angiogenic efficiency of ASCs. It was concluded that implantation of hypoxic ASCs or SPIOASCs promotes therapeutic angiogenesis and cardiac function recovery in the infarcted myocardium. SPIO labeling does not impact the beneficial effect of hypoxic ASCs.展开更多
This study established superparamagnetic iron oxide (SPIO)-labeled nerve growth factor-β (NGF-β) gene-modified spinal cord-derived neural stem cells (NSCs). The E14 rat embryonic spinal cord-derived NSCs were isolat...This study established superparamagnetic iron oxide (SPIO)-labeled nerve growth factor-β (NGF-β) gene-modified spinal cord-derived neural stem cells (NSCs). The E14 rat embryonic spinal cord-derived NSCs were isolated and cultured. The cells of the third passage were transfected with plasmid pcDNA3-hNGFβ by using FuGENE HD transfection reagent. The expression of NGF-β was measured by immunocytochemistry and Western blotting. The positive clones were selected, allowed to proliferate and then labeled with SPIO, which was mediated by FuGENE HD transfection reagent. Prussian blue staining and transmission electron microscopy (TEM) were used to identify the SPIO particles in the cells. The distinctive markers for stem cells (nestin), neuron (β-Ⅲ-tubulin), oli-godendrocyte (CNPase) and astrocyte (GFAP) were employed to evaluate the differentiation ability of the labeled cells. The immunocytochemistry and western blotting showed that NGF-β was expressed in spinal cordderived NSCs. Prussian blue staining indicated that numerous blue-stained particles appeared in the cytoplasma of the labeled cells. TEM showed that SPIO particles were found in vacuolar structures of different sizes and the cytoplasma. The immunocytochemistry demonstrated that the labeled cells were nestin-positive. After differentiation, the cells expressed β-III-tubulin, CNPase and GFAP. It was concluded that the SPIO-labeled NGF-β gene-modified spinal cord-derived NSC were successfully established, which are multipotent and capable of self-renewal.展开更多
Objective: To study the growth and differentiation of superparamagnetic iron oxides(SPIOs) labeled neural stem cells(NSCs).Methods: After NSCs were cultured and subcultured from newborn rat brain,they were magneticall...Objective: To study the growth and differentiation of superparamagnetic iron oxides(SPIOs) labeled neural stem cells(NSCs).Methods: After NSCs were cultured and subcultured from newborn rat brain,they were magnetically labeled with ferumoxides(a kind of SPIOs).Growth,differentiation and other biology properties of the cells were investigated with immunocytochemistry,transmission electron microscopy(TEM) and Prussian blue staining.Results: Nestin positive cells were found in the culture and offspring clones.NSCs could be differentiated into positive GFAP and NF200 cells in serum culture.When NSCs incubated with ferumoxides,the iron particles were seen in intracellular as well as in offspring clones.With the increase in concentration of ferumoxides(5.6-11.2 μg/ml),ferumoxides showed no significant difference effects on the growth and differentiation of NSCs.When the concentration of ferumoxides exceeded 22.4 μg/ml,there was significant difference(P<0.05). Conclusion: We successfully label NSCs with ferumoxides,it is useful for tracking of magnetic labeled NSCs in vivo with MRI.展开更多
Recent progress of the preparation and applications of superparamagnetic iron oxide(SPIO) clusters as magnetic resonance imaging(MRI) probes is reviewed with regard to their applications in labeling and tracking cells...Recent progress of the preparation and applications of superparamagnetic iron oxide(SPIO) clusters as magnetic resonance imaging(MRI) probes is reviewed with regard to their applications in labeling and tracking cells in vivo, in diagnosis of cardiovascular diseases and tumors, and in drug delivery systems. Magnetic nanoparticles(NPs), especially SPIO nanoparticles, have long been used as MRI contrast agents and as an advantageous nanoplatform for drug delivery,taking advantage of their unique magnetic properties and ability to function at the molecular and cellular levels. Due to advances in nanotechnology, various means to control SPIO NPs' size, composition, magnetization and relaxivity have been developed, as well as ways to usefully modify their surface. Recently, self-assembly of SPIO NP clusters in particulate carriers — such as polymeric micelles, vesicles, liposomes, and layer-by-layer(Lb L) capsules — have been widely studied for application as ultrasensitive MRI probes, owing to their remarkably high spin–spin(T2) relaxivity and convenience for further functionalization.展开更多
超顺磁性氧化铁纳米粒子(superparamagnetic iron oxide nanoparticle, SPION)由于其独特的性质,如低毒、生物相容性、强大的磁性,以及在多功能模式中的优越作用,在肿瘤诊断、构建多模态肿瘤分子影像探针及治疗方面展现出巨大的潜力,今...超顺磁性氧化铁纳米粒子(superparamagnetic iron oxide nanoparticle, SPION)由于其独特的性质,如低毒、生物相容性、强大的磁性,以及在多功能模式中的优越作用,在肿瘤诊断、构建多模态肿瘤分子影像探针及治疗方面展现出巨大的潜力,今后可以在临床上提高肿瘤诊断的特异性、敏感性,实现诊疗一体化,本文从SPION的成像机制、合成方法出发,阐述近年来SPION在肿瘤的各种靶向成像、多模态成像和治疗方面的研究进展,展望未来SPION在肿瘤诊断及治疗中的发展前景,旨在为更好地构建基于SPION的新型诊疗一体化肿瘤探针提供参考。展开更多
Superparamagnetic iron oxide(SPIO)nanoparticles have become a popular strategy of cancer treatment and molecular imaging because of their versatile properties and biocompatibility.A variety of studies have shown the e...Superparamagnetic iron oxide(SPIO)nanoparticles have become a popular strategy of cancer treatment and molecular imaging because of their versatile properties and biocompatibility.A variety of studies have shown the exciting potential of functionalized SPIO nanoparticles,such as surface-coated,targeted ligandconjugated,and/or drug-loaded SPIO nanoparticles,as powerful tools for targeted imaging and therapy.Moreover,the applications of SPIO nanoparticles that integrate diagnosis and therapy in SPIO nanoparticles facilitate the monitoring of therapeutic efficacy during treatment.In the present review,we primarily concentrate on the recent advancements in the field of SPIO nanoparticles in terms of synthesis,targeted therapy,and cancer imaging.展开更多
基金Supported by National Natural Science Foundation of China(32060228)。
文摘Superparamagnetic iron oxide nanoparticles(SPIONs)have immeasurable potentials in many fields such as nanobiotechnology and biomedical engineering because of their superparamagnetic properties and small particle size.This review introduces the methods for SPIONs synthesis,including co-precipitation,thermal decomposition,microemulsion and hydrothermal reaction,and surface modification of SPIONs with organometallic and inorganic metals,surface modification for targeted drug delivery,and the use of SPIONs as a contrast agent.In addition,this article also provides an overview of recent progress in SPIONs for the treatment of glioma,lung cancer and breast cancer.
文摘Five types of superparamagnetic iron oxide (SPIO),i.e. Ferumoxides (Feridex? Ⅳ, Berlex Laboratories),Fe r u c a r b o t ra n ( Re s ov i s t?, B aye r H e a l t h c a re ) ,Ferumoxtran-10 (AMI-227 or Code-7227, Combidex?, AMAG Pharma; Sinerem?, Guerbet), NC100150(Clariscan?, Nycomed,) and (VSOP C184, Ferropharm)have been designed and clinically tested as magneticresonance contrast agents. However, until nowResovist? is current available in only a few countries.The other four agents have been stopped for furtherdevelopment or withdrawn from the market. AnotherSPIO agent Ferumoxytol (Feraheme) is approved forthe treatment of iron deficiency in adult chronic kidneydisease patients. Ferumoxytol is comprised of ironoxide particles surrounded by a carbohydrate coat, andit is being explored as a potential imaging approach forevaluating lymph nodes and certain liver tumors.
基金the National Natural Science Foundation of China(Nos.81402640,81502816)the Natural Science Foundation of Hubei Province(No.2014CFB406)+1 种基金the Health and Family Planning Commission of Wuhan City(No.WX15B23)Training Plan for Young and Middleaged Backbone Talents in Wuhan[No.2014(77)].
文摘Biodistribution and toxicity assessment are critical for safe clinical use of newly developed medicines.Superparamagnetic iron oxide nanoparticles (SPION)are effective carriers for targeted drug delivery.This study aimed to examine the toxicity and biodistribution of SPION coated with polyethylenimine (PEI)(SPION-PEI)designed for small interfering RNA (siRNA) delivery both in vitro and in vivo.SPION-PEI/siRNA complexes were prepared at different weight ratios.Cytotoxic effects of SPION-PEI/siRNA on HSC-T6 cell viability were determined by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT).Rats were divided into three groups:a control group,a normal-saline group and a SPION-PEI/siRNA group.After a single intravenous injection,in vivo nanoparticle biodistribution and accumulation were evaluated by Prussian blue staining in the heart,liver,spleen,lung and kidney 8 h,24 h,and 7 days after the injection.Their distribution was histologically studied at the three time points by measuring ironpositive areas (μm2)in organ sections stained with Prussian blue.The same organs were analyzed by H&E staining for any possible histopathological changes.Furthermore,biochemical indexes such as alanine amino transaminase (ALT),aspartate transaminase (AST),blood urea nitrogen (BUN)and creatinine (CREA)were also assessed at all experimental time points.Electrophoresis exhibited that the SPION-PEI could retard siRNA altogether at weight ratios above 4.MTT assay showed that SPION-PEI loaded with siRNA had low cytotoxicity.In vivo study revealed that the liver and spleen were the major sites of SPION-PEI/siRNA deposition.The iron content was significantly increased in the liver and spleen,peaking 24 h after intravenous injection and then declining gradually.No evidence was found of irreversible histopathological damage to any of the organs tested.These results suggested that most SPION-PEI/siRNA complexes were distributed in the liver and spleen,which might be the target organs of SPION-PEI/siRNA complexes.SPION- PEI/siRNA may serve as in vivo carrier for biomedical medicines.
基金Project supported by the Major State Basic Research Development Program of China(Grant Nos.2013CB733802 and 2014CB744503)the National Natural Science Foundation of China(Grant Nos.81101101 and 51273165)+1 种基金the Key Project of Chinese Ministry of Education(Grant No.212149)the Fundamental Research Funds for the Central Universities,China(Grant Nos.2013121039 and ZK1002)
文摘Superparamagnetic iron oxide nanoparticles(SPIONs) are one of the most versatile and safe nanoparticles in a wide variety of biomedical applications. In the past decades, considerable efforts have been made to investigate the potential adverse biological effects and safety issues associated with SPIONs, which is essential for the development of next-generation SPIONs and for continued progress in translational research. In this mini review, we summarize recent developments in toxicity studies on SPIONs, focusing on the relationship between the physicochemical properties of SPIONs and their induced toxic biological responses for a better toxicological understanding of SPIONs.
基金supported by a grant from the National Natural Sciences Foundation of China (No. 30870639)
文摘To assess a novel cell manipulation technique of tissue engineering with respect to its ability to augment superparamagnetic iron oxide particles (SPIO) labeled mesenchymal stem cells (MSCs) density at a localized cartilage defect site in an in vitro phantom by applying magnetic force. Meanwhile, non-invasive imaging techniques were use to track SPIO-labeled MSCs by magnetic resonance imaging (MRI). Human bone marrow MSCs were cultured and labeled with SPIO. Fresh degenerated human osteochondral fragments were obtained during total knee arthroplasty and a cartilage defect was created at the center. Then, the osteochondral fragments were attached to the sidewalls of culture flasks filled with phosphate-buffered saline (PBS) to mimic the human joint cavity. The SPIO-labeled MSCs were injected into the culture flasks in the presence of a 0.57 Tesla (T) magnetic force. Before and 90 min after cell targeting, the specimens underwent T2-weighted turbo spin-echo (SET2WI) sequence of 3.0 T MRI. MRI results were compared with histological findings. Macroscopic observation showed that SPIO-labeled MSCs were steered to the target region of cartilage defect. MRI revealed significant changes in signal intensity (P<0.01). HE staining exibited that a great number of MSCs formed a three-dimensional (3D) cell "sheet" structure at the chondral defect site. It was concluded that 0.57 T magnetic force permits spatial delivery of magnetically labeled MSCs to the target region in vitro. High-field MRI can serve as an very sensitive non-invasive technique for the visualization of SPIO-labeled MSCs.
基金supported by Deutsche Forschungsgemeinschaft(DFG)grant Klinische Forschergruppe(KFO)213(to JG).
文摘Due to their very small size,nanoparticles can interact with all cells in the central nervous system.One of the most promising nanoparticle subgroups are very small superparamagnetic iron oxide nanoparticles(VSOP)that are citrate coated for electrostatic stabilization.To determine their influence on murine blood-derived monocytes,which easily enter the injured central nervous system,we applied VSOP and carboxydextran-coated superparamagnetic iron oxide nanoparticles(Resovist).We assessed their impact on the viability,cytokine,and chemokine secretion,as well as iron uptake of murine blood-derived monocytes.We found that(1)the monocytes accumulated VSOP and Resovist,(2)this uptake seemed to be nanoparticle-and time-dependent,(3)the decrease of monocytes viability was treatment-related,(4)VSOP and Resovist incubation did not alter cytokine homeostasis,and(5)overall a 6-hour treatment with 0.75 mM VSOP-R1 was probably sufficient to effectively label monocytes for future experiments.Since homeostasis is not altered,it is safe to label blood-derived monocles with VSOP.VSOP labeled monocytes can be used to study injured central nervous system sites further,for example with drug-carrying VSOP.
文摘Background: The aim of this study was to investigate the distribution of the c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles in the major organs of mice by MR imaging. Methods: Sixty BALB/c mice were randomly divided into experimental and control groups. MR scans were performed in each mouse of the experimental group at five different time points (10, 30, 60, 180 and 360 min) after injection of the antisense probe. The signal from each major organ (liver, spleen, heart, kidney and muscle tissue) in comparison with the background signal (signal to noise ratio) was determined at each time point as a measure of the distribution of the antisense probe. Six control mice were killed at each of the same time points and the organs immediately removed for determination of their iron content. Results: After injection of the antisense probe, the highest enrichment of the probe was seen in the spleen, reaching a peak at 180 min, followed by the liver, muscle, heart and kidney. Conclusions: MR imaging can visualize the distribution of c-erbB2 antisense probe labeled with superparamagnetic iron oxide nanoparticles in the major organs of mice, and this may provide the basis for further in vivo studies of MR imaging time and dose selection.
基金supported by the National Natural Science Foundation of China(No.81200105)the Scientific Research Foundation of Wuhan Union Hospital(No.02.03.2017-34)+3 种基金the Natural Science Foundation of Hubei Province of China(No.2015CFB457)the China Postdoctoral Science Foundation(No.20100470050)Canadian Institute of Health Research(CIHR)(No.200806RMF-189873-RMC-CDAA-42533)National Research Council of Canada(NRC)
文摘Adipose-derived stem cells(ASCs) induce therapeutic angiogenesis due to pro-angiogenic cytokines secretion. Superparamagnetic iron oxide(SPIO) nanoparticles are critical for magnetic resonance(MR) tracking of implanted cells. Hypoxia is a powerful stimulus for angiogenic activity of ASCs. In this study, we investigated whether therapeutic potency could be enhanced by implantation of hypoxia-preconditioned SPIO-labeled ASCs(SPIOASCs) into the infarcted myocardium. ASCs and SPIOASCs were cultured under 2% O_2(hypoxia) or 95% air(normoxia). Cells were intramyocardially injected into the infarcted myocardium after 48-h culture. We found that hypoxia culture increased the m RNA expression of hypoxia-inducible factor-1 alpha(HIF-1α) and vascular endothelial growth factor(VEGF) in ASCs and SPIOASCs. The VEGF protein in the conditioned medium was significantly higher in hypoxic ASCs and SPIOASCs than in normoxic ASCs and SPIOASCs. The capillary density and left ventricular contractile function in the infarcted myocardium were significantly higher 4 weeks after implantation with hypoxic ASCs and SPIOASCs than with normoxic ASCs and SPIOASCs. Improvement in the capillary density and left ventricle function didn't differ between hypoxic ASCs-transplanted rats and hypoxic SPIOASCs-transplanted rats. Hypoxic culture enhanced the angiogenic efficiency of ASCs. It was concluded that implantation of hypoxic ASCs or SPIOASCs promotes therapeutic angiogenesis and cardiac function recovery in the infarcted myocardium. SPIO labeling does not impact the beneficial effect of hypoxic ASCs.
基金supported by a grant from the National Natural Sciences Foundation of China (No.30672151)
文摘This study established superparamagnetic iron oxide (SPIO)-labeled nerve growth factor-β (NGF-β) gene-modified spinal cord-derived neural stem cells (NSCs). The E14 rat embryonic spinal cord-derived NSCs were isolated and cultured. The cells of the third passage were transfected with plasmid pcDNA3-hNGFβ by using FuGENE HD transfection reagent. The expression of NGF-β was measured by immunocytochemistry and Western blotting. The positive clones were selected, allowed to proliferate and then labeled with SPIO, which was mediated by FuGENE HD transfection reagent. Prussian blue staining and transmission electron microscopy (TEM) were used to identify the SPIO particles in the cells. The distinctive markers for stem cells (nestin), neuron (β-Ⅲ-tubulin), oli-godendrocyte (CNPase) and astrocyte (GFAP) were employed to evaluate the differentiation ability of the labeled cells. The immunocytochemistry and western blotting showed that NGF-β was expressed in spinal cordderived NSCs. Prussian blue staining indicated that numerous blue-stained particles appeared in the cytoplasma of the labeled cells. TEM showed that SPIO particles were found in vacuolar structures of different sizes and the cytoplasma. The immunocytochemistry demonstrated that the labeled cells were nestin-positive. After differentiation, the cells expressed β-III-tubulin, CNPase and GFAP. It was concluded that the SPIO-labeled NGF-β gene-modified spinal cord-derived NSC were successfully established, which are multipotent and capable of self-renewal.
基金Supported by National Natural Science Foundation of Chi-na (330370500)Postdoctoral Science Foundation of China(2003033363)the CQUMS Excellent Doctoral Founda-tion
文摘Objective: To study the growth and differentiation of superparamagnetic iron oxides(SPIOs) labeled neural stem cells(NSCs).Methods: After NSCs were cultured and subcultured from newborn rat brain,they were magnetically labeled with ferumoxides(a kind of SPIOs).Growth,differentiation and other biology properties of the cells were investigated with immunocytochemistry,transmission electron microscopy(TEM) and Prussian blue staining.Results: Nestin positive cells were found in the culture and offspring clones.NSCs could be differentiated into positive GFAP and NF200 cells in serum culture.When NSCs incubated with ferumoxides,the iron particles were seen in intracellular as well as in offspring clones.With the increase in concentration of ferumoxides(5.6-11.2 μg/ml),ferumoxides showed no significant difference effects on the growth and differentiation of NSCs.When the concentration of ferumoxides exceeded 22.4 μg/ml,there was significant difference(P<0.05). Conclusion: We successfully label NSCs with ferumoxides,it is useful for tracking of magnetic labeled NSCs in vivo with MRI.
基金Project supported by the National Key Basic Research Program of China(Grant No.2013CB933903)the National Natural Science Foundation of China(Grant Nos.20974065+2 种基金51173117and 50830107)the Scientific Research Start-up Fund of Kunming University of Science and Technology(Grant No.KKSY201305089)
文摘Recent progress of the preparation and applications of superparamagnetic iron oxide(SPIO) clusters as magnetic resonance imaging(MRI) probes is reviewed with regard to their applications in labeling and tracking cells in vivo, in diagnosis of cardiovascular diseases and tumors, and in drug delivery systems. Magnetic nanoparticles(NPs), especially SPIO nanoparticles, have long been used as MRI contrast agents and as an advantageous nanoplatform for drug delivery,taking advantage of their unique magnetic properties and ability to function at the molecular and cellular levels. Due to advances in nanotechnology, various means to control SPIO NPs' size, composition, magnetization and relaxivity have been developed, as well as ways to usefully modify their surface. Recently, self-assembly of SPIO NP clusters in particulate carriers — such as polymeric micelles, vesicles, liposomes, and layer-by-layer(Lb L) capsules — have been widely studied for application as ultrasensitive MRI probes, owing to their remarkably high spin–spin(T2) relaxivity and convenience for further functionalization.
文摘超顺磁性氧化铁纳米粒子(superparamagnetic iron oxide nanoparticle, SPION)由于其独特的性质,如低毒、生物相容性、强大的磁性,以及在多功能模式中的优越作用,在肿瘤诊断、构建多模态肿瘤分子影像探针及治疗方面展现出巨大的潜力,今后可以在临床上提高肿瘤诊断的特异性、敏感性,实现诊疗一体化,本文从SPION的成像机制、合成方法出发,阐述近年来SPION在肿瘤的各种靶向成像、多模态成像和治疗方面的研究进展,展望未来SPION在肿瘤诊断及治疗中的发展前景,旨在为更好地构建基于SPION的新型诊疗一体化肿瘤探针提供参考。
基金This work was supported by the Natural Science Foundation of Hubei Province(Grant No.2009HBKJH1).
文摘Superparamagnetic iron oxide(SPIO)nanoparticles have become a popular strategy of cancer treatment and molecular imaging because of their versatile properties and biocompatibility.A variety of studies have shown the exciting potential of functionalized SPIO nanoparticles,such as surface-coated,targeted ligandconjugated,and/or drug-loaded SPIO nanoparticles,as powerful tools for targeted imaging and therapy.Moreover,the applications of SPIO nanoparticles that integrate diagnosis and therapy in SPIO nanoparticles facilitate the monitoring of therapeutic efficacy during treatment.In the present review,we primarily concentrate on the recent advancements in the field of SPIO nanoparticles in terms of synthesis,targeted therapy,and cancer imaging.