Japanese encephalitis virus （jEV） is a mosquito-borne virus of the family Flaviviridae. It is the causative agent of Japanese encephalitis with approximately 50,000 infection cases and 10,000 fatal cases annually in...Japanese encephalitis virus （jEV） is a mosquito-borne virus of the family Flaviviridae. It is the causative agent of Japanese encephalitis with approximately 50,000 infection cases and 10,000 fatal cases annually in Asia （Erlanger et al., 2009）. Although liveattenuated JEV vaccine has been developed and used for human and pig vaccination, JE occurs epidemically or sporadically in some developing countries or even in vaccinated areas （Solomon, 2006）. Host resistance factors play an important role in the outcome of viral infection.展开更多
Objective: To explore the changes and significance of tumor suppressor gene p53 in primary hepatocellu-lar carcinoma (PHC ) with hepatitis B virus (HBV ) infection. Methods: Tumor tissues and surrounding nontumortissu...Objective: To explore the changes and significance of tumor suppressor gene p53 in primary hepatocellu-lar carcinoma (PHC ) with hepatitis B virus (HBV ) infection. Methods: Tumor tissues and surrounding nontumortissues of sixteen PHC cases were studied by Southern hybridization to detect the state of HBV-DNA in tissues, byimmunohistochemical staining to determine HBsAg, HBxAg and p53 protein, and by PCR directed sequencing toanalyse the point mutation of p53 gene exons 5 to 8. Results: Among the 16 cases. 13 cases were HBV-DNA posi-tive, 10 tumor cases and 13 nontumor tissues cases HBxAg positive, and 9 cases posltive for p53 protein. The se-quencing of p53 gene point mutation was found in 5 cases, only one of which was sited at codon 249 G to T. Con-clusion: The mutation of p53 gene codon 249 is infrequent in HBV related PHC,indicating the accumulation of p53protein in cells may be associated with expression of HBxAg. HBxAg binding to p53 protein and inactivation of p53function play important roles in the development of PHC.展开更多
基金supported by the National Natural Science Foundation of China(Nos.81371814 and 31302116)National Program on Key Research Project of China(No.2016YFD0500400)the Program of International S&T Cooperation(No.2014DFE30140)
文摘Japanese encephalitis virus （jEV） is a mosquito-borne virus of the family Flaviviridae. It is the causative agent of Japanese encephalitis with approximately 50,000 infection cases and 10,000 fatal cases annually in Asia （Erlanger et al., 2009）. Although liveattenuated JEV vaccine has been developed and used for human and pig vaccination, JE occurs epidemically or sporadically in some developing countries or even in vaccinated areas （Solomon, 2006）. Host resistance factors play an important role in the outcome of viral infection.
文摘Objective: To explore the changes and significance of tumor suppressor gene p53 in primary hepatocellu-lar carcinoma (PHC ) with hepatitis B virus (HBV ) infection. Methods: Tumor tissues and surrounding nontumortissues of sixteen PHC cases were studied by Southern hybridization to detect the state of HBV-DNA in tissues, byimmunohistochemical staining to determine HBsAg, HBxAg and p53 protein, and by PCR directed sequencing toanalyse the point mutation of p53 gene exons 5 to 8. Results: Among the 16 cases. 13 cases were HBV-DNA posi-tive, 10 tumor cases and 13 nontumor tissues cases HBxAg positive, and 9 cases posltive for p53 protein. The se-quencing of p53 gene point mutation was found in 5 cases, only one of which was sited at codon 249 G to T. Con-clusion: The mutation of p53 gene codon 249 is infrequent in HBV related PHC,indicating the accumulation of p53protein in cells may be associated with expression of HBxAg. HBxAg binding to p53 protein and inactivation of p53function play important roles in the development of PHC.
