Cotton fiber is one of the main raw materials for the textile industry.In recent years,many cotton fiber quality QTL have been identified,but few were applied in breeding.In this study,a genome wide association study(...Cotton fiber is one of the main raw materials for the textile industry.In recent years,many cotton fiber quality QTL have been identified,but few were applied in breeding.In this study,a genome wide association study(GWAS)of fiber-quality traits in 265 upland cotton breeding intermediate lines(GhBreeding),combined with genome-wide selective sweep analysis(GSSA)and genomic selection(GS),revealed 25 QTL.Most of these QTL were ignored by only using GWAS.The CRISPR/Cas9 mutants of GhMYB_D13 had shorter fiber,which indicates the credibility of QTL to a certain extent.Then these QTL were verified in other cotton natural populations,5 stable QTL were found having broad potential for application in breeding.Additionally,among these 5 stable QTL,superior genotypes of 4 showed an enrichment in most improved new varieties widely cultivated currently.These findings provide insights for how to identify more QTL through combined multiple genomic analysis to apply in breeding.展开更多
3D eikonal equation is a partial differential equation for the calculation of first-arrival traveltimes and has been widely applied in many scopes such as ray tracing,source localization,reflection migration,seismic m...3D eikonal equation is a partial differential equation for the calculation of first-arrival traveltimes and has been widely applied in many scopes such as ray tracing,source localization,reflection migration,seismic monitoring and tomographic imaging.In recent years,many advanced methods have been developed to solve the 3D eikonal equation in heterogeneous media.However,there are still challenges for the stable and accurate calculation of first-arrival traveltimes in 3D strongly inhomogeneous media.In this paper,we propose an adaptive finite-difference(AFD)method to numerically solve the 3D eikonal equation.The novel method makes full use of the advantages of different local operators characterizing different seismic wave types to calculate factors and traveltimes,and then the most accurate factor and traveltime are adaptively selected for the convergent updating based on the Fermat principle.Combined with global fast sweeping describing seismic waves propagating along eight directions in 3D media,our novel method can achieve the robust calculation of first-arrival traveltimes with high precision at grid points either near source point or far away from source point even in a velocity model with large and sharp contrasts.Several numerical examples show the good performance of the AFD method,which will be beneficial to many scientific applications.展开更多
Fixed-point fast sweeping methods are a class of explicit iterative methods developed in the literature to efficiently solve steady-state solutions of hyperbolic partial differential equations(PDEs).As other types of ...Fixed-point fast sweeping methods are a class of explicit iterative methods developed in the literature to efficiently solve steady-state solutions of hyperbolic partial differential equations(PDEs).As other types of fast sweeping schemes,fixed-point fast sweeping methods use the Gauss-Seidel iterations and alternating sweeping strategy to cover characteristics of hyperbolic PDEs in a certain direction simultaneously in each sweeping order.The resulting iterative schemes have a fast convergence rate to steady-state solutions.Moreover,an advantage of fixed-point fast sweeping methods over other types of fast sweeping methods is that they are explicit and do not involve the inverse operation of any nonlinear local system.Hence,they are robust and flexible,and have been combined with high-order accurate weighted essentially non-oscillatory(WENO)schemes to solve various hyperbolic PDEs in the literature.For multidimensional nonlinear problems,high-order fixed-point fast sweeping WENO methods still require quite a large amount of computational costs.In this technical note,we apply sparse-grid techniques,an effective approximation tool for multidimensional problems,to fixed-point fast sweeping WENO methods for reducing their computational costs.Here,we focus on fixed-point fast sweeping WENO schemes with third-order accuracy(Zhang et al.2006[41]),for solving Eikonal equations,an important class of static Hamilton-Jacobi(H-J)equations.Numerical experiments on solving multidimensional Eikonal equations and a more general static H-J equation are performed to show that the sparse-grid computations of the fixed-point fast sweeping WENO schemes achieve large savings of CPU times on refined meshes,and at the same time maintain comparable accuracy and resolution with those on corresponding regular single grids.展开更多
In the heterogeneous reservoirs,CO_(2) flooding easily leads to CO_(2) gas channeling,which can seriously affect sweeping efficiency and reduce oil recovery.After thoroughly investigating the advantages and shortcomin...In the heterogeneous reservoirs,CO_(2) flooding easily leads to CO_(2) gas channeling,which can seriously affect sweeping efficiency and reduce oil recovery.After thoroughly investigating the advantages and shortcomings of various CO_(2) plugging technologies,this paper focuses on the feasibility of improving conventional water-alternating gas(WAG)through CO_(2)-responsive gel materials.Based on the different chemical reaction mechanisms between the unique chemical structure and CO_(2),changes in the material’s physical and chemical properties can respond to CO_(2).The feasibility of utilizing these property changes for CO_(2)-responsive plugging is explored.Various CO_(2)-responsive gels and gel nanoparticles have been extensively researched in different fields,such as energy,medicine,and biology.This paper surveys the molecular structures,chemical compositions,response mechanisms,and changes of these CO_(2)-responsive gels,aiming to draw insights into the carbon dioxide-enhanced oil recovery(CO_(2)-EOR)field.Finally,the key issues and future development direction of CO_(2)-responsive plugging gels were analyzed.展开更多
This review presents our perspective on the factors that have brought polymer flooding to its current state. Insights are provided on why HPAM is the dominant polymer used as well as what is needed to make alternative...This review presents our perspective on the factors that have brought polymer flooding to its current state. Insights are provided on why HPAM is the dominant polymer used as well as what is needed to make alternative polymers and mobility-control methods viable. Explanation is given for why large polymer banks are needed for polymer flooding, and design of the injected polymer viscosity is detailed for cases with/without crossflow. The role of fractures and horizontal wells are discussed for improving injectivity and extending polymer flooding to recover oils with viscosities as high as 10,000 cP. Operational improvements are described to minimize mechanical and oxidative stability to allow HPAM polymers to be viable to 70 °C and ATBS polymers to 120 °C. Key factors affecting polymer retention are summarized. The paper points out unresolved issues and future directions for polymer flooding.展开更多
In addition to the temperature and pressure conditions,the pore fluid composition and migration behavior are also crucial to control hydrate decomposition in the exploitation process.In this work,to investigate the ef...In addition to the temperature and pressure conditions,the pore fluid composition and migration behavior are also crucial to control hydrate decomposition in the exploitation process.In this work,to investigate the effects of these factors,a series of depressurization experiments were carried out in a visible one-dimensional reactor,using hydrate reservoir samples with water saturations ranging from 20%to 65%.The results showed a linear relationship between gas production rates and gas saturations of the reservoir,suggesting that a larger gas-phase space was conducive to hydrate decomposition and gas outflow.Therefore,the rapid water production in the early stage of hydrate exploitation could release more gas-phase space in the water-rich reservoir,which in turn improved the gas production efficiency.Meanwhile,the spatiotemporal evolution of pore fluids could lead to partial accelerated decomposition or secondary formation of hydrates.In the unsealed reservoir,the peripheral water infiltration kept reservoir at a high water saturation,which hindered the overall production process and caused higher water production.Importantly,depressurization assisted with the N2 sweep could displace the pore water rapidly.According to the results,it is recommended that using the short-term N2 sweep as an auxiliary means in the early stage of depressurization to expand the gas-phase space in order to achieve the highest production efficiency.展开更多
Background The genomes of worldwide poultry breeds divergently selected for performance and other phenotypic traits may also be affected by,and formed due to,past and current admixture events.Adaptation to diverse env...Background The genomes of worldwide poultry breeds divergently selected for performance and other phenotypic traits may also be affected by,and formed due to,past and current admixture events.Adaptation to diverse environments,including acclimation to harsh climatic conditions,has also left selection footprints in breed genomes.Results Using the Chicken 50K_CobbCons SNP chip,we genotyped four divergently selected breeds:two aboriginal,cold tolerant Ushanka and Orloff Mille Fleur,one egg-type Russian White subjected to artificial selection for cold tolerance,and one meat-type White Cornish.Signals of selective sweeps were determined in the studied breeds using three methods:(1)assessment of runs of homozygosity islands,(2)F_(ST) based population differential analysis,and(3)haplotype differentiation analysis.Genomic regions of true selection signatures were identified by two or more methods or in two or more breeds.In these regions,we detected 540 prioritized candidate genes supplemented them with those that occurred in one breed using one statistic and were suggested in other studies.Amongst them,SOX5,ME3,ZNF536,WWP1,RIPK2,OSGIN2,DECR1,TPO,PPARGC1A,BDNF,MSTN,and beta-keratin genes can be especially mentioned as candidates for cold adaptation.Epigenetic factors may be involved in regulating some of these important genes(e.g.,TPO and BDNF).Conclusion Based on a genome-wide scan,our findings can help dissect the genetic architecture underlying various phenotypic traits in chicken breeds.These include genes representing the sine qua non for adaptation to harsh environments.Cold tolerance in acclimated chicken breeds may be developed following one of few specific gene expression mechanisms or more than one overlapping response known in cold-exposed individuals,and this warrants further investigation.展开更多
Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wid...Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wide association analyses combined with meta-analyses,Bayesian analyses,and selective sweep analyses were performed to screen single nucleotide polymorphisms(SNPs)and other genetic loci that were significantly associated with egg number traits in 11,279 chickens from seven material lines.Results:Yellow-feathered meat-type chickens laid 115 eggs at 43 weeks of age and white-feathered chickens laid 143 eggs at 60 weeks of age,with heritability ranging from 0.034–0.258.Based on meta-analyses and selective sweep analyses,one region(10.81–13.05 Mb)on chromosome Z was associated with egg number in all lines.Further analyses using the W2 line was also associated with the same region,and 29 SNPs were identified that significantly affected estimation of breeding value of egg numbers.The 29 SNPs were identified as having a significant effect on the egg number EBV in 3194 birds in line W2.There are 36 genes in the region,with glial cell derived neurotrophic factor,DAB adaptor protein 2,protein kinase AMP-activated catalytic subunit alpha 1,NAD kinase 2,mitochondrial,WD repeat domain 70,leukemia inhibitory factor receptor alpha,complement C6,and complement C7 identified as being potentially affecting to egg number.In addition,three SNPs(rs318154184,rs13769886,and rs313325646)associated with egg number were located on or near the prolactin receptor gene.Conclusion:Our study used genomic information from different chicken lines and populations to identify a genomic region(spanning 2.24 Mb)associated with egg number.Nine genes and 29 SNPs were identified as the most likely candidate genes and variations for egg production.These results contribute to the identification of candidate genes and variants for egg traits in poultry.展开更多
Enhancing host immunity is an effective way to reduce morbidity in chickens.Heterophil to lymphocyte ratio(H/L)is associated with host disease resistance in birds.Chickens with different H/L levels show different dise...Enhancing host immunity is an effective way to reduce morbidity in chickens.Heterophil to lymphocyte ratio(H/L)is associated with host disease resistance in birds.Chickens with different H/L levels show different disease resistances.However,the utility of the H/L as an indicator of immune function needs to be further analyzed.In this study,a H/L directional breeding chicken line(Jingxing yellow chicken)was constructed,which has been bred for 12 generations.We compared the function of heterophils,and combined statistical analysis to explore the candidate genes and pathways related to H/L.The oxidative burst function of the heterophils isolated from the H/L selection line(G12)was increased(P=0.044)compared to the non-selection line(NS).The 22.44 Mb genomic regions which annotated 300 protein-coding genes were selected in the genome of G9(n=92)compared to NS(n=92)based on a genome-wide selective sweep.Several selective regions were identified containing genes like interferon induced with helicase C domain 1(IFIH1)and moesin(MSN)associated with the intracellular receptor signaling pathway,C–C motif chemokine receptor 6(CCR6),dipeptidyl peptidase 4(DPP4)and hemolytic complement(HC)associated with the negative regulation of leukocyte chemotaxis and tight junction protein 1(TJP1)associated with actin cytoskeleton organization.In addition,45 genome-wide significant indels containing 29 protein-coding genes were also identified as associated with the H/L based on genome-wide association study(GWAS).The expression of protein tyrosine phosphatase non-receptor type 5(PTPN5)(r=0.75,P=0.033)and oxysterol binding protein like 5(OSBPL5)(r=0.89,P=0.0027)were positively correlated with H/L.Compared to the high H/L group,the expressions of PTPN5 and OSBPL5 were decreased(P<0.05)in the low H/L group of Beijing you chicken.The A/A allelic frequency of indel 5_13108985(P=3.85E–06)within OSBPL5 gradually increased from the NS to G5 and G9,and the individuals with A/A exhibited lower H/L than individuals with heterozygote A/ATCT(P=4.28E–04)and homozygous ATCT/ATCT(P=3.40E–05).Above results indicated oxidative burst function of heterophils were enhanced,and 22.44 Mb genomic regions were selected with the directional selection of H/L.In addition,PTPN5 and OSBPL5 genes were identified as H/L-related candidate genes.These findings revealed the complex genetic mechanism of H/L related to immunity and will allow selection for improving chicken immunity based on the H/L.展开更多
Background Sharply increased beef consumption is propelling the genetic improvement projects of beef cattle in China.Three-dimensional genome structure is confirmed to be an important layer of transcription regulation...Background Sharply increased beef consumption is propelling the genetic improvement projects of beef cattle in China.Three-dimensional genome structure is confirmed to be an important layer of transcription regulation.Although genome-wide interaction data of several livestock species have already been produced,the genome structure states and its regulatory rules in cattle muscle are still limited.Results Here we present the first 3D genome data in Longissimus dorsi muscle of fetal and adult cattle(Bos taurus).We showed that compartments,topologically associating domains(TADs),and loop undergo re-organization and the structure dynamics were consistent with transcriptomic divergence during muscle development.Furthermore,we annotated cis-regulatory elements in cattle genome during myogenesis and demonstrated the enrichments of promoter and enhancer in selection sweeps.We further validated the regulatory function of one HMGA2 intronic enhancer near a strong sweep region on primary bovine myoblast proliferation.Conclusions Our data provide key insights of the regulatory function of high order chromatin structure and cattle myogenic biology,which will benefit the progress of genetic improvement of beef cattle.展开更多
The 60-meter band range is tremendously useful in telecommunication,military and governmental applications.The I.T.U.(International Telecommunication Union)required isolationism to former radio frequency services beca...The 60-meter band range is tremendously useful in telecommunication,military and governmental applications.The I.T.U.(International Telecommunication Union)required isolationism to former radio frequency services because the various frequency bands are extremely overloaded.The allocation of new frequency bands are a lengthy procedure as well as time taking.As a result,the researchers use bidirectional,amateur radio frequency communication for 60-meter band,usually the frequency slot of 5250-5450 KHz,although the entire band is not essentially obtainable for all countries.For transmission and reception of these frequencies,a local oscillator is used in the mixer unit to generate the local signal for mixing the input and reference signals.For this function different type of oscillators are used.In this paper,a three-stage ring oscillator is designed with 1 V supply.Ring oscillators(RO)is the base to explore like to identifying,specify with modelling resources in the disparity in behaviour of the circuit in terms of industrialized design and layout parameters.This type of oscillators are free from noise as inductor is not used to the circuit as in LC oscillator,Heartly oscillator,Colpitt and tuned oscillators.The present approach of circuit designing,the scaling of CMOS(Complementary Metal Oxide Semiconductor)transistor will moderate,the procedure variability.In the forthcoming article,a ring oscillator with fixed capacitor(1 pF)and with variable capacitors(1 to 100 pF)is analysed.The frequency analysis with different capacitor is performed.The total delay of 3-stage oscillator is 4.82 ns with 5.2 MHz oscillation frequency.The overall Power dissipation of the circuit is 1.852μWat 1 V supply.The simulation analysis is performed on 45 nm CMOS technology with both transistor width are 278 and 420 nm.展开更多
Fixed-point fast sweeping WENO methods are a class of efficient high-order numerical methods to solve steady-state solutions of hyperbolic partial differential equations(PDEs).The Gauss-Seidel iterations and alternati...Fixed-point fast sweeping WENO methods are a class of efficient high-order numerical methods to solve steady-state solutions of hyperbolic partial differential equations(PDEs).The Gauss-Seidel iterations and alternating sweeping strategy are used to cover characteristics of hyperbolic PDEs in each sweeping order to achieve fast convergence rate to steady-state solutions.A nice property of fixed-point fast sweeping WENO methods which distinguishes them from other fast sweeping methods is that they are explicit and do not require inverse operation of nonlinear local systems.Hence,they are easy to be applied to a general hyperbolic system.To deal with the difficulties associated with numerical boundary treatment when high-order finite difference methods on a Cartesian mesh are used to solve hyperbolic PDEs on complex domains,inverse Lax-Wendroff(ILW)procedures were developed as a very effective approach in the literature.In this paper,we combine a fifthorder fixed-point fast sweeping WENO method with an ILW procedure to solve steadystate solution of hyperbolic conservation laws on complex computing regions.Numerical experiments are performed to test the method in solving various problems including the cases with the physical boundary not aligned with the grids.Numerical results show highorder accuracy and good performance of the method.Furthermore,the method is compared with the popular third-order total variation diminishing Runge-Kutta(TVD-RK3)time-marching method for steady-state computations.Numerical examples show that for most of examples,the fixed-point fast sweeping method saves more than half CPU time costs than TVD-RK3 to converge to steady-state solutions.展开更多
As an active flow control technology and with the advantages of no moving components, the Sweeping jet actuator has become a hotspot in the field of flow control. However, the linear relationship between oscillation f...As an active flow control technology and with the advantages of no moving components, the Sweeping jet actuator has become a hotspot in the field of flow control. However, the linear relationship between oscillation frequency and momentum coefficient in a sweeping jet actuator makes it difficult to determine the dominant factors that affect control effectiveness. Decoupling the oscillation frequency and momentum coefficient, as well as determining the control mechanism, is the focus of studying the sweeping jet actuator. In this study, a novel sweeping jet actuator is designed using synthetic jets instead of feedback channels and applied to the flow separation control of NACA0018 airfoil. This article studies the control effect under three oscillation frequencies of F<sup>+</sup> = f × c/U<sub>∞</sub> = 1, 10, 100 and three momentum coefficients of C<sub>μ</sub> = 0.45%, 0.625%, 0.9%. The numerical results indicate that all three oscillation frequencies have good control effects on flow separation, and the control effect is best when F<sup>+</sup> = 1, with the maximum lift coefficient increasing by approximately 14% compared to the other two cases. And the sweeping jet actuator has a better ability to control flow separation as the momentum coefficient increases. By decoupling the characteristics of the sweeping jet actuator and conducting numerical analysis of the flow control effect, it will promote its better engineering application in the field of flow control. .展开更多
采用熔融石英毛细管,以含有50mm o l/L十二烷基硫酸钠的50mm o l/L硼酸盐缓冲液为电极缓冲液,以10mm o l/L硼酸盐缓冲液为上样缓冲液,经过对分离条件的优化,成功地建立了胶束电动毛细管色谱结合在线sw eep ing(推扫)富集技术检测中性脂...采用熔融石英毛细管,以含有50mm o l/L十二烷基硫酸钠的50mm o l/L硼酸盐缓冲液为电极缓冲液,以10mm o l/L硼酸盐缓冲液为上样缓冲液,经过对分离条件的优化,成功地建立了胶束电动毛细管色谱结合在线sw eep ing(推扫)富集技术检测中性脂溶性物质旋覆花内酯(acety lb ritann ilac tone,ABL)的实验方法。所建方法的批内、批间测定值的相对标准偏差均小于5%,灵敏度为0.005g/L,回收率大于92%;被检测样品的含量与峰面积呈良好的线性关系,相关系数为0.997 5。用所建立的方法检测了旋覆花素中ABL的含量及其在体内的动态变化,结果表明胶束电动毛细管色谱结合在线sw eep ing样品富集技术可显著提高检测的灵敏度。该方法具有操作简单、进样量小(nL级)、检测速度快等特点,弥补了毛细管电泳在测定痕量组分方面的不足。展开更多
基金supported by National Key Research and Development Program of China(2022YFF1001400)the National Natural Science Foundation of China(31830062 and 32172071)+1 种基金Innovation and Application of Superior Crop Germplasm Resources of Shihezi(2021NY01)Breeding of New Cotton Varieties and Application of Transgenic Breeding Technology(2022NY01)。
文摘Cotton fiber is one of the main raw materials for the textile industry.In recent years,many cotton fiber quality QTL have been identified,but few were applied in breeding.In this study,a genome wide association study(GWAS)of fiber-quality traits in 265 upland cotton breeding intermediate lines(GhBreeding),combined with genome-wide selective sweep analysis(GSSA)and genomic selection(GS),revealed 25 QTL.Most of these QTL were ignored by only using GWAS.The CRISPR/Cas9 mutants of GhMYB_D13 had shorter fiber,which indicates the credibility of QTL to a certain extent.Then these QTL were verified in other cotton natural populations,5 stable QTL were found having broad potential for application in breeding.Additionally,among these 5 stable QTL,superior genotypes of 4 showed an enrichment in most improved new varieties widely cultivated currently.These findings provide insights for how to identify more QTL through combined multiple genomic analysis to apply in breeding.
基金The authors thank the funds supported by the China National Nuclear Corporation under Grants Nos.WUQNYC2101 and WUHTLM2101-04National Natural Science Foundation of China(42074132,42274154).
文摘3D eikonal equation is a partial differential equation for the calculation of first-arrival traveltimes and has been widely applied in many scopes such as ray tracing,source localization,reflection migration,seismic monitoring and tomographic imaging.In recent years,many advanced methods have been developed to solve the 3D eikonal equation in heterogeneous media.However,there are still challenges for the stable and accurate calculation of first-arrival traveltimes in 3D strongly inhomogeneous media.In this paper,we propose an adaptive finite-difference(AFD)method to numerically solve the 3D eikonal equation.The novel method makes full use of the advantages of different local operators characterizing different seismic wave types to calculate factors and traveltimes,and then the most accurate factor and traveltime are adaptively selected for the convergent updating based on the Fermat principle.Combined with global fast sweeping describing seismic waves propagating along eight directions in 3D media,our novel method can achieve the robust calculation of first-arrival traveltimes with high precision at grid points either near source point or far away from source point even in a velocity model with large and sharp contrasts.Several numerical examples show the good performance of the AFD method,which will be beneficial to many scientific applications.
文摘Fixed-point fast sweeping methods are a class of explicit iterative methods developed in the literature to efficiently solve steady-state solutions of hyperbolic partial differential equations(PDEs).As other types of fast sweeping schemes,fixed-point fast sweeping methods use the Gauss-Seidel iterations and alternating sweeping strategy to cover characteristics of hyperbolic PDEs in a certain direction simultaneously in each sweeping order.The resulting iterative schemes have a fast convergence rate to steady-state solutions.Moreover,an advantage of fixed-point fast sweeping methods over other types of fast sweeping methods is that they are explicit and do not involve the inverse operation of any nonlinear local system.Hence,they are robust and flexible,and have been combined with high-order accurate weighted essentially non-oscillatory(WENO)schemes to solve various hyperbolic PDEs in the literature.For multidimensional nonlinear problems,high-order fixed-point fast sweeping WENO methods still require quite a large amount of computational costs.In this technical note,we apply sparse-grid techniques,an effective approximation tool for multidimensional problems,to fixed-point fast sweeping WENO methods for reducing their computational costs.Here,we focus on fixed-point fast sweeping WENO schemes with third-order accuracy(Zhang et al.2006[41]),for solving Eikonal equations,an important class of static Hamilton-Jacobi(H-J)equations.Numerical experiments on solving multidimensional Eikonal equations and a more general static H-J equation are performed to show that the sparse-grid computations of the fixed-point fast sweeping WENO schemes achieve large savings of CPU times on refined meshes,and at the same time maintain comparable accuracy and resolution with those on corresponding regular single grids.
基金Supported by the Open Fund Project of Hubei Key Laboratory of Oil and Gas Drilling and Production Engineering(YQZC202105)Yangtze University Student Innovation Program(Yz2022018).
文摘In the heterogeneous reservoirs,CO_(2) flooding easily leads to CO_(2) gas channeling,which can seriously affect sweeping efficiency and reduce oil recovery.After thoroughly investigating the advantages and shortcomings of various CO_(2) plugging technologies,this paper focuses on the feasibility of improving conventional water-alternating gas(WAG)through CO_(2)-responsive gel materials.Based on the different chemical reaction mechanisms between the unique chemical structure and CO_(2),changes in the material’s physical and chemical properties can respond to CO_(2).The feasibility of utilizing these property changes for CO_(2)-responsive plugging is explored.Various CO_(2)-responsive gels and gel nanoparticles have been extensively researched in different fields,such as energy,medicine,and biology.This paper surveys the molecular structures,chemical compositions,response mechanisms,and changes of these CO_(2)-responsive gels,aiming to draw insights into the carbon dioxide-enhanced oil recovery(CO_(2)-EOR)field.Finally,the key issues and future development direction of CO_(2)-responsive plugging gels were analyzed.
文摘This review presents our perspective on the factors that have brought polymer flooding to its current state. Insights are provided on why HPAM is the dominant polymer used as well as what is needed to make alternative polymers and mobility-control methods viable. Explanation is given for why large polymer banks are needed for polymer flooding, and design of the injected polymer viscosity is detailed for cases with/without crossflow. The role of fractures and horizontal wells are discussed for improving injectivity and extending polymer flooding to recover oils with viscosities as high as 10,000 cP. Operational improvements are described to minimize mechanical and oxidative stability to allow HPAM polymers to be viable to 70 °C and ATBS polymers to 120 °C. Key factors affecting polymer retention are summarized. The paper points out unresolved issues and future directions for polymer flooding.
基金financially supported by the National Natural Science Foundation of China,China(Nos.52004136,22127812,U20B6005)China Postdoctoral Science Foundation,China(Nos.2020M670347,2021T140382)Guangdong MEPP Fund(No.GDNRC[2021]055).
文摘In addition to the temperature and pressure conditions,the pore fluid composition and migration behavior are also crucial to control hydrate decomposition in the exploitation process.In this work,to investigate the effects of these factors,a series of depressurization experiments were carried out in a visible one-dimensional reactor,using hydrate reservoir samples with water saturations ranging from 20%to 65%.The results showed a linear relationship between gas production rates and gas saturations of the reservoir,suggesting that a larger gas-phase space was conducive to hydrate decomposition and gas outflow.Therefore,the rapid water production in the early stage of hydrate exploitation could release more gas-phase space in the water-rich reservoir,which in turn improved the gas production efficiency.Meanwhile,the spatiotemporal evolution of pore fluids could lead to partial accelerated decomposition or secondary formation of hydrates.In the unsealed reservoir,the peripheral water infiltration kept reservoir at a high water saturation,which hindered the overall production process and caused higher water production.Importantly,depressurization assisted with the N2 sweep could displace the pore water rapidly.According to the results,it is recommended that using the short-term N2 sweep as an auxiliary means in the early stage of depressurization to expand the gas-phase space in order to achieve the highest production efficiency.
基金supported by the Russian Science Foundation within the Project No.21-66-00007support of the Russian Ministry of Science and Higher Education。
文摘Background The genomes of worldwide poultry breeds divergently selected for performance and other phenotypic traits may also be affected by,and formed due to,past and current admixture events.Adaptation to diverse environments,including acclimation to harsh climatic conditions,has also left selection footprints in breed genomes.Results Using the Chicken 50K_CobbCons SNP chip,we genotyped four divergently selected breeds:two aboriginal,cold tolerant Ushanka and Orloff Mille Fleur,one egg-type Russian White subjected to artificial selection for cold tolerance,and one meat-type White Cornish.Signals of selective sweeps were determined in the studied breeds using three methods:(1)assessment of runs of homozygosity islands,(2)F_(ST) based population differential analysis,and(3)haplotype differentiation analysis.Genomic regions of true selection signatures were identified by two or more methods or in two or more breeds.In these regions,we detected 540 prioritized candidate genes supplemented them with those that occurred in one breed using one statistic and were suggested in other studies.Amongst them,SOX5,ME3,ZNF536,WWP1,RIPK2,OSGIN2,DECR1,TPO,PPARGC1A,BDNF,MSTN,and beta-keratin genes can be especially mentioned as candidates for cold adaptation.Epigenetic factors may be involved in regulating some of these important genes(e.g.,TPO and BDNF).Conclusion Based on a genome-wide scan,our findings can help dissect the genetic architecture underlying various phenotypic traits in chicken breeds.These include genes representing the sine qua non for adaptation to harsh environments.Cold tolerance in acclimated chicken breeds may be developed following one of few specific gene expression mechanisms or more than one overlapping response known in cold-exposed individuals,and this warrants further investigation.
基金the Guangdong Provincial Key Research and Development Program(2020B02022002)the Shandong Agricultural Seed Improvement Project(2020LZGC013)the Technological Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202005)。
文摘Background:Egg production is economically important in the meat-type chicken industry.To better understand the molecular genetic mechanism of egg production in meat-type chicken,genetic parameter estimation,genome-wide association analyses combined with meta-analyses,Bayesian analyses,and selective sweep analyses were performed to screen single nucleotide polymorphisms(SNPs)and other genetic loci that were significantly associated with egg number traits in 11,279 chickens from seven material lines.Results:Yellow-feathered meat-type chickens laid 115 eggs at 43 weeks of age and white-feathered chickens laid 143 eggs at 60 weeks of age,with heritability ranging from 0.034–0.258.Based on meta-analyses and selective sweep analyses,one region(10.81–13.05 Mb)on chromosome Z was associated with egg number in all lines.Further analyses using the W2 line was also associated with the same region,and 29 SNPs were identified that significantly affected estimation of breeding value of egg numbers.The 29 SNPs were identified as having a significant effect on the egg number EBV in 3194 birds in line W2.There are 36 genes in the region,with glial cell derived neurotrophic factor,DAB adaptor protein 2,protein kinase AMP-activated catalytic subunit alpha 1,NAD kinase 2,mitochondrial,WD repeat domain 70,leukemia inhibitory factor receptor alpha,complement C6,and complement C7 identified as being potentially affecting to egg number.In addition,three SNPs(rs318154184,rs13769886,and rs313325646)associated with egg number were located on or near the prolactin receptor gene.Conclusion:Our study used genomic information from different chicken lines and populations to identify a genomic region(spanning 2.24 Mb)associated with egg number.Nine genes and 29 SNPs were identified as the most likely candidate genes and variations for egg production.These results contribute to the identification of candidate genes and variants for egg traits in poultry.
基金supported by the grants from the National Natural Science Foundation of China(32072708)the National Key R&D Program of China(2018YFE0128000)the Major Scientific Research Projects of Chinese Academy of Agricultural Sciences(CAAS-ZDRW202005).
文摘Enhancing host immunity is an effective way to reduce morbidity in chickens.Heterophil to lymphocyte ratio(H/L)is associated with host disease resistance in birds.Chickens with different H/L levels show different disease resistances.However,the utility of the H/L as an indicator of immune function needs to be further analyzed.In this study,a H/L directional breeding chicken line(Jingxing yellow chicken)was constructed,which has been bred for 12 generations.We compared the function of heterophils,and combined statistical analysis to explore the candidate genes and pathways related to H/L.The oxidative burst function of the heterophils isolated from the H/L selection line(G12)was increased(P=0.044)compared to the non-selection line(NS).The 22.44 Mb genomic regions which annotated 300 protein-coding genes were selected in the genome of G9(n=92)compared to NS(n=92)based on a genome-wide selective sweep.Several selective regions were identified containing genes like interferon induced with helicase C domain 1(IFIH1)and moesin(MSN)associated with the intracellular receptor signaling pathway,C–C motif chemokine receptor 6(CCR6),dipeptidyl peptidase 4(DPP4)and hemolytic complement(HC)associated with the negative regulation of leukocyte chemotaxis and tight junction protein 1(TJP1)associated with actin cytoskeleton organization.In addition,45 genome-wide significant indels containing 29 protein-coding genes were also identified as associated with the H/L based on genome-wide association study(GWAS).The expression of protein tyrosine phosphatase non-receptor type 5(PTPN5)(r=0.75,P=0.033)and oxysterol binding protein like 5(OSBPL5)(r=0.89,P=0.0027)were positively correlated with H/L.Compared to the high H/L group,the expressions of PTPN5 and OSBPL5 were decreased(P<0.05)in the low H/L group of Beijing you chicken.The A/A allelic frequency of indel 5_13108985(P=3.85E–06)within OSBPL5 gradually increased from the NS to G5 and G9,and the individuals with A/A exhibited lower H/L than individuals with heterozygote A/ATCT(P=4.28E–04)and homozygous ATCT/ATCT(P=3.40E–05).Above results indicated oxidative burst function of heterophils were enhanced,and 22.44 Mb genomic regions were selected with the directional selection of H/L.In addition,PTPN5 and OSBPL5 genes were identified as H/L-related candidate genes.These findings revealed the complex genetic mechanism of H/L related to immunity and will allow selection for improving chicken immunity based on the H/L.
基金supported by the National Natural Science Foundation of China[Grant No.31972558]the Agricultural Improved Seed Project of Shandong Province[Grant No.2020LZGC014-03]。
文摘Background Sharply increased beef consumption is propelling the genetic improvement projects of beef cattle in China.Three-dimensional genome structure is confirmed to be an important layer of transcription regulation.Although genome-wide interaction data of several livestock species have already been produced,the genome structure states and its regulatory rules in cattle muscle are still limited.Results Here we present the first 3D genome data in Longissimus dorsi muscle of fetal and adult cattle(Bos taurus).We showed that compartments,topologically associating domains(TADs),and loop undergo re-organization and the structure dynamics were consistent with transcriptomic divergence during muscle development.Furthermore,we annotated cis-regulatory elements in cattle genome during myogenesis and demonstrated the enrichments of promoter and enhancer in selection sweeps.We further validated the regulatory function of one HMGA2 intronic enhancer near a strong sweep region on primary bovine myoblast proliferation.Conclusions Our data provide key insights of the regulatory function of high order chromatin structure and cattle myogenic biology,which will benefit the progress of genetic improvement of beef cattle.
文摘The 60-meter band range is tremendously useful in telecommunication,military and governmental applications.The I.T.U.(International Telecommunication Union)required isolationism to former radio frequency services because the various frequency bands are extremely overloaded.The allocation of new frequency bands are a lengthy procedure as well as time taking.As a result,the researchers use bidirectional,amateur radio frequency communication for 60-meter band,usually the frequency slot of 5250-5450 KHz,although the entire band is not essentially obtainable for all countries.For transmission and reception of these frequencies,a local oscillator is used in the mixer unit to generate the local signal for mixing the input and reference signals.For this function different type of oscillators are used.In this paper,a three-stage ring oscillator is designed with 1 V supply.Ring oscillators(RO)is the base to explore like to identifying,specify with modelling resources in the disparity in behaviour of the circuit in terms of industrialized design and layout parameters.This type of oscillators are free from noise as inductor is not used to the circuit as in LC oscillator,Heartly oscillator,Colpitt and tuned oscillators.The present approach of circuit designing,the scaling of CMOS(Complementary Metal Oxide Semiconductor)transistor will moderate,the procedure variability.In the forthcoming article,a ring oscillator with fixed capacitor(1 pF)and with variable capacitors(1 to 100 pF)is analysed.The frequency analysis with different capacitor is performed.The total delay of 3-stage oscillator is 4.82 ns with 5.2 MHz oscillation frequency.The overall Power dissipation of the circuit is 1.852μWat 1 V supply.The simulation analysis is performed on 45 nm CMOS technology with both transistor width are 278 and 420 nm.
基金Research was supported by the NSFC Grant 11872210Research was supported by the NSFC Grant 11872210 and Grant No.MCMS-I-0120G01+1 种基金Research supported in part by the AFOSR Grant FA9550-20-1-0055NSF Grant DMS-2010107.
文摘Fixed-point fast sweeping WENO methods are a class of efficient high-order numerical methods to solve steady-state solutions of hyperbolic partial differential equations(PDEs).The Gauss-Seidel iterations and alternating sweeping strategy are used to cover characteristics of hyperbolic PDEs in each sweeping order to achieve fast convergence rate to steady-state solutions.A nice property of fixed-point fast sweeping WENO methods which distinguishes them from other fast sweeping methods is that they are explicit and do not require inverse operation of nonlinear local systems.Hence,they are easy to be applied to a general hyperbolic system.To deal with the difficulties associated with numerical boundary treatment when high-order finite difference methods on a Cartesian mesh are used to solve hyperbolic PDEs on complex domains,inverse Lax-Wendroff(ILW)procedures were developed as a very effective approach in the literature.In this paper,we combine a fifthorder fixed-point fast sweeping WENO method with an ILW procedure to solve steadystate solution of hyperbolic conservation laws on complex computing regions.Numerical experiments are performed to test the method in solving various problems including the cases with the physical boundary not aligned with the grids.Numerical results show highorder accuracy and good performance of the method.Furthermore,the method is compared with the popular third-order total variation diminishing Runge-Kutta(TVD-RK3)time-marching method for steady-state computations.Numerical examples show that for most of examples,the fixed-point fast sweeping method saves more than half CPU time costs than TVD-RK3 to converge to steady-state solutions.
文摘As an active flow control technology and with the advantages of no moving components, the Sweeping jet actuator has become a hotspot in the field of flow control. However, the linear relationship between oscillation frequency and momentum coefficient in a sweeping jet actuator makes it difficult to determine the dominant factors that affect control effectiveness. Decoupling the oscillation frequency and momentum coefficient, as well as determining the control mechanism, is the focus of studying the sweeping jet actuator. In this study, a novel sweeping jet actuator is designed using synthetic jets instead of feedback channels and applied to the flow separation control of NACA0018 airfoil. This article studies the control effect under three oscillation frequencies of F<sup>+</sup> = f × c/U<sub>∞</sub> = 1, 10, 100 and three momentum coefficients of C<sub>μ</sub> = 0.45%, 0.625%, 0.9%. The numerical results indicate that all three oscillation frequencies have good control effects on flow separation, and the control effect is best when F<sup>+</sup> = 1, with the maximum lift coefficient increasing by approximately 14% compared to the other two cases. And the sweeping jet actuator has a better ability to control flow separation as the momentum coefficient increases. By decoupling the characteristics of the sweeping jet actuator and conducting numerical analysis of the flow control effect, it will promote its better engineering application in the field of flow control. .