Background:Primary biliary cholangitis(PBC)is a chronic biliary autoimmune liver disease characterized by intrahepatic cholestasis.Swertia mussotii Franch.(SMF)is a Tibetan medicine with hepatoprotective and anti-infl...Background:Primary biliary cholangitis(PBC)is a chronic biliary autoimmune liver disease characterized by intrahepatic cholestasis.Swertia mussotii Franch.(SMF)is a Tibetan medicine with hepatoprotective and anti-inflammatory activities.In this study,the therapeutic effect and potential mechanisms of SMF on PBC were investigated by bioinformatics analysis and in vitro experimental validation,with the aim of promoting the progress of SMF and PBC research.Methods:We first explored the therapeutic effects and key targets of SMF on PBC using a network pharmacology approach,further screened the core targets using the GSE79850 dataset,and finally validated the results using molecular docking techniques and in vitro experiments.Results:By bioinformatics analysis,we identified core targets of SMF for PBC treatment(STAT3,JAK2,TNF-α,and IL-1β)and important signaling pathways:JAK-STAT,TNF,and PI3K-AKT.The molecular docking results showed that the significant components of SMF had good binding properties to the core targets.In vitro experiments showed that SMF extracts improved the extent of epithelial-mesenchymal transition in human intrahepatic biliary epithelial cells and had a significant reversal effect on epithelial-mesenchymal transition process markers and potential targets in PBC.Conclusion:SMF may exert its therapeutic effects on PBC by acting on important targets such as STAT3,JAK2,TNF-α,IL-1β,Vimentin,and E-cadherin and the pathways in which they are involved.展开更多
Two new C19-diterpenoid alkaloids, davidisines A （1） and B （2） along with thirteen known alkaloids were isolated from the whole herb of Delphinium davidii Franch. Their structures were established by spectral meth...Two new C19-diterpenoid alkaloids, davidisines A （1） and B （2） along with thirteen known alkaloids were isolated from the whole herb of Delphinium davidii Franch. Their structures were established by spectral methods, especially 2D NMR techniques.展开更多
【目的】为了阐明吲哚丁酸(IBA)诱导青榨槭不定根发生的激素调控机制。【方法】依据青榨槭扦插生根过程的解剖学观察,分别于对照期和4个生根关键时期采集插穗生根部位树皮组织进行IBA处理下的比较转录组学研究和生理指标检测。【结果】...【目的】为了阐明吲哚丁酸(IBA)诱导青榨槭不定根发生的激素调控机制。【方法】依据青榨槭扦插生根过程的解剖学观察,分别于对照期和4个生根关键时期采集插穗生根部位树皮组织进行IBA处理下的比较转录组学研究和生理指标检测。【结果】4个对比组分别鉴定到6774、6256、4522、4571个差异基因。GO(Gene ontoloy)富集分析发现,碳水化合物代谢和不定根发育等条目在不定根表达和伸长阶段高度富集。KEGG(Kyoto encyclopedia of genes and genomes)富集分析显示参与玉米素、油菜素内酯生物合成以及植物激素信号转导途径的差异基因发挥着重要作用。激素含量检测结果表明,高浓度的生长素参与了不定根的诱导过程,而相对低水平的生长素有利于其后期发育;细胞分裂素和玉米素均在愈伤组织形成期出现转运峰;油菜素内酯在生根初期被显著诱导。【结论】IBA刺激青榨槭不定根启动与增强生长素运输相关,不定根形成早期需要一定水平的细胞分裂素积累才能促进生根,玉米素和油菜素内酯的生物合成促进了不定根原基的诱导和启动。展开更多
基金supported by the Key project of Chinese Academy of Sciences(Grant No.ZDRW-ZS-2020-2)Innovation Platform Program of Qinghai Province(2021-ZJ-T02),Key Laboratory Project of Qinghai Province(2022-ZJ-Y05)+1 种基金the Natural Science Foundation of China(Grant No.82171863)China Postdoctoral Science Foundation funded project(2021M701642).
文摘Background:Primary biliary cholangitis(PBC)is a chronic biliary autoimmune liver disease characterized by intrahepatic cholestasis.Swertia mussotii Franch.(SMF)is a Tibetan medicine with hepatoprotective and anti-inflammatory activities.In this study,the therapeutic effect and potential mechanisms of SMF on PBC were investigated by bioinformatics analysis and in vitro experimental validation,with the aim of promoting the progress of SMF and PBC research.Methods:We first explored the therapeutic effects and key targets of SMF on PBC using a network pharmacology approach,further screened the core targets using the GSE79850 dataset,and finally validated the results using molecular docking techniques and in vitro experiments.Results:By bioinformatics analysis,we identified core targets of SMF for PBC treatment(STAT3,JAK2,TNF-α,and IL-1β)and important signaling pathways:JAK-STAT,TNF,and PI3K-AKT.The molecular docking results showed that the significant components of SMF had good binding properties to the core targets.In vitro experiments showed that SMF extracts improved the extent of epithelial-mesenchymal transition in human intrahepatic biliary epithelial cells and had a significant reversal effect on epithelial-mesenchymal transition process markers and potential targets in PBC.Conclusion:SMF may exert its therapeutic effects on PBC by acting on important targets such as STAT3,JAK2,TNF-α,IL-1β,Vimentin,and E-cadherin and the pathways in which they are involved.
文摘Two new C19-diterpenoid alkaloids, davidisines A （1） and B （2） along with thirteen known alkaloids were isolated from the whole herb of Delphinium davidii Franch. Their structures were established by spectral methods, especially 2D NMR techniques.
文摘【目的】为了阐明吲哚丁酸(IBA)诱导青榨槭不定根发生的激素调控机制。【方法】依据青榨槭扦插生根过程的解剖学观察,分别于对照期和4个生根关键时期采集插穗生根部位树皮组织进行IBA处理下的比较转录组学研究和生理指标检测。【结果】4个对比组分别鉴定到6774、6256、4522、4571个差异基因。GO(Gene ontoloy)富集分析发现,碳水化合物代谢和不定根发育等条目在不定根表达和伸长阶段高度富集。KEGG(Kyoto encyclopedia of genes and genomes)富集分析显示参与玉米素、油菜素内酯生物合成以及植物激素信号转导途径的差异基因发挥着重要作用。激素含量检测结果表明,高浓度的生长素参与了不定根的诱导过程,而相对低水平的生长素有利于其后期发育;细胞分裂素和玉米素均在愈伤组织形成期出现转运峰;油菜素内酯在生根初期被显著诱导。【结论】IBA刺激青榨槭不定根启动与增强生长素运输相关,不定根形成早期需要一定水平的细胞分裂素积累才能促进生根,玉米素和油菜素内酯的生物合成促进了不定根原基的诱导和启动。
