Objective:This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common traditional Chinese medicine(TCM)Syndromes—damp heat syndrome and liver depression and spleen de...Objective:This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common traditional Chinese medicine(TCM)Syndromes—damp heat syndrome and liver depression and spleen deficiency syndrome.Methods:According to the diagnostic criteria of Western medicine,TCM and screening exclusion criteria,65 cases of CHB with damp heat syndrome and 28 cases of CHB with liver depression and spleen deficiency syndrome were finally included in the study.All the basic information was gathered and the fresh fecal samples were collected for 16S rDNA sequencing.16S rDNA of gut microbiota was sequenced using Illumina hiseq 2,500 high-throughput sequencing platform.Based on the optimized sequence,Operational Taxonomic Units(OTU)clustering analysis and taxonomic annotation were carried out.Results:The difference in relative abundance of gut microbiota was significant between damp heat syndrome and liver depression and spleen deficiency syndrome in CHB patients.Cyanobacteria was only found in damp heat syndrome.The relative abundance of Erysipelotrichia and Subdoligranulum were higher in liver depression and spleen deficiency syndrome,while the relative abundance of Rhodospirillales,Alphaproteobacteria and Lachnospira were higher in the damp heat syndrome.LDA Effect Size(LEfSe)analysis showed that Lachnospira,Olsenella and Subdoligranulum had significant difference in species among the two TCM syndromes.Conclusion:The different characteristics of gut microbiota in the two TCM syndromes of CHB patients may play an important role in syndrome formation of TCM,which provides a new field of vision for the accurate diagnosis and treatment of TCM.展开更多
目的通过文献研究,分析原发性开角型青光眼(primary open angle glaucoma,POAG)证型分布特点及其证候、证素特点,为临床POAG的诊治提供依据。方法检索CNKI(中国知网数据库)、VIP(维普数据库)、CBM(中国生物医学文献数据库)和Wanfang数据...目的通过文献研究,分析原发性开角型青光眼(primary open angle glaucoma,POAG)证型分布特点及其证候、证素特点,为临床POAG的诊治提供依据。方法检索CNKI(中国知网数据库)、VIP(维普数据库)、CBM(中国生物医学文献数据库)和Wanfang数据库(万方数据库),采用EpiData3.1软件建立POAG证候文献数据库,应用统计软件SPSS 19.0进行统计分析。结果最终纳入文献11篇,包括644例不同证型的患者,规范后的POAG证型11种,最常见的4种证型为:肝肾亏虚证(26.55%)、肝郁化火证(23.45%)、痰湿泛目证(18.94%)、肝郁气滞证(13.20%),与POAG相关的病位证素为肝,病性证素主要与阴虚、血虚、阳亢、气滞相关。结论本研究初步反映了POAG常见证型、证候分布特点,并对其证素特点进行了概括,为临床POAG的诊疗提供依据。展开更多
基金The Sixth Group of National Senior Chinese Medicine Experts’ Academic Experience Inheritance Project [approval:State Administration of Traditional Chinese Medicine (2017) No.29]the Foshan High Level Medical Key Discipline Construction Project and "Peak Climbing Plan" for Foshan High Level Hospital Construction[approval:Foshan Municipal Government Office,No. 2019 (01)]
文摘Objective:This study was designed to explore the characteristics of gut microbiota in CHB patients with two most common traditional Chinese medicine(TCM)Syndromes—damp heat syndrome and liver depression and spleen deficiency syndrome.Methods:According to the diagnostic criteria of Western medicine,TCM and screening exclusion criteria,65 cases of CHB with damp heat syndrome and 28 cases of CHB with liver depression and spleen deficiency syndrome were finally included in the study.All the basic information was gathered and the fresh fecal samples were collected for 16S rDNA sequencing.16S rDNA of gut microbiota was sequenced using Illumina hiseq 2,500 high-throughput sequencing platform.Based on the optimized sequence,Operational Taxonomic Units(OTU)clustering analysis and taxonomic annotation were carried out.Results:The difference in relative abundance of gut microbiota was significant between damp heat syndrome and liver depression and spleen deficiency syndrome in CHB patients.Cyanobacteria was only found in damp heat syndrome.The relative abundance of Erysipelotrichia and Subdoligranulum were higher in liver depression and spleen deficiency syndrome,while the relative abundance of Rhodospirillales,Alphaproteobacteria and Lachnospira were higher in the damp heat syndrome.LDA Effect Size(LEfSe)analysis showed that Lachnospira,Olsenella and Subdoligranulum had significant difference in species among the two TCM syndromes.Conclusion:The different characteristics of gut microbiota in the two TCM syndromes of CHB patients may play an important role in syndrome formation of TCM,which provides a new field of vision for the accurate diagnosis and treatment of TCM.
文摘目的通过文献研究,分析原发性开角型青光眼(primary open angle glaucoma,POAG)证型分布特点及其证候、证素特点,为临床POAG的诊治提供依据。方法检索CNKI(中国知网数据库)、VIP(维普数据库)、CBM(中国生物医学文献数据库)和Wanfang数据库(万方数据库),采用EpiData3.1软件建立POAG证候文献数据库,应用统计软件SPSS 19.0进行统计分析。结果最终纳入文献11篇,包括644例不同证型的患者,规范后的POAG证型11种,最常见的4种证型为:肝肾亏虚证(26.55%)、肝郁化火证(23.45%)、痰湿泛目证(18.94%)、肝郁气滞证(13.20%),与POAG相关的病位证素为肝,病性证素主要与阴虚、血虚、阳亢、气滞相关。结论本研究初步反映了POAG常见证型、证候分布特点,并对其证素特点进行了概括,为临床POAG的诊疗提供依据。
