The Gram-positive model bacterium Bacillus subtilis,has been broadly applied in various fields because of its low pathogenicity and strong protein secretion ability,as well as its well-developed fermentation technolog...The Gram-positive model bacterium Bacillus subtilis,has been broadly applied in various fields because of its low pathogenicity and strong protein secretion ability,as well as its well-developed fermentation technology.B.subtilis is considered as an attractive host in the field of metabolic engineering,in particular for protein expression and secretion,so it has been well studied and applied in genetic engineering.In this review,we discussed why B.subtilis is a good chassis cell for metabolic engineering.We also summarized the latest research progress in systematic biology,synthetic biology and evolution-based engineering of B.subtilis,and showed systemic metabolic engineering expedite the harnessing B.subtilis for bioproduction.展开更多
基金This work was supported by the National Key R&D Program of China(2018YFA0900300,2018YFD0901001)the National Natural Science Foundation of China(NSFC 31800086,31900052)+1 种基金the Tianjin Science Fund for Distinguished Young Scholars(17JCJQJC45300)the Science and Technology Service Network(STS)Initiative of the Chinese Academy of Sciences(CAS)(KFJ-STS-ZDTP-065).
文摘The Gram-positive model bacterium Bacillus subtilis,has been broadly applied in various fields because of its low pathogenicity and strong protein secretion ability,as well as its well-developed fermentation technology.B.subtilis is considered as an attractive host in the field of metabolic engineering,in particular for protein expression and secretion,so it has been well studied and applied in genetic engineering.In this review,we discussed why B.subtilis is a good chassis cell for metabolic engineering.We also summarized the latest research progress in systematic biology,synthetic biology and evolution-based engineering of B.subtilis,and showed systemic metabolic engineering expedite the harnessing B.subtilis for bioproduction.
