MicroRNAs(miRNAs)play a significant role in axon regeneration following spinal cord injury.However,the functions of numerous miRNAs in axon regeneration within the central nervous system(CNS)remain largely unexplored....MicroRNAs(miRNAs)play a significant role in axon regeneration following spinal cord injury.However,the functions of numerous miRNAs in axon regeneration within the central nervous system(CNS)remain largely unexplored.Here,we elucidate the positive role of microRNA-2184(miR-2184)in axon regeneration within zebrafish Mauthner cells(M-cells).The upregulation of miR-2184 in a single M-cell can facilitate axon regeneration,while the specific sponge-induced silencing of miR-2184 leads to impeded regeneration.We show that syt3,a downstream target of miR-2184,negatively regulates axon regeneration,and the regeneration suppression modulated by syt3 depends on its binding to Ca2+.Furthermore,pharmacological stimulation of the cAMP/PKA pathway suggests that changes in the readily releasable pool may affect axon regeneration.Our data indicate that miR-2184 promotes axon regeneration of M-cells within the CNS by modulating the downstream target syt3,providing valuable insights into potential therapeutic strategies.展开更多
Synaptotagmins （Syts） are a large family of integral membrane proteins that regulate synaptic function and membrane traffcking. Emerging evidences show involvement of Syts in human diseases. Here, we review the rece...Synaptotagmins （Syts） are a large family of integral membrane proteins that regulate synaptic function and membrane traffcking. Emerging evidences show involvement of Syts in human diseases. Here, we review the recent studies of several Syts （Syt1, 2, 7, 11, and 14） in the pathophysiological mechanisms of neurodegeneration disorders such as Alzheimer’s disease, Parkinson’s disease, and attention-defcit/hyperactivity disorder etc. A better understanding of the diverse physiological and pathological functions of different Syt isoforms is needed for potential therapeutic interventions in the future.展开更多
基金supported by the Research Funds of the Center for Advanced Interdisciplinary Science and Biomedicine of IHM(QYZD20220002)the National Natural Science Foundation of China(82071357)the Ministry of Science and Technology of China(2019YFA0405600 to B.H.).
文摘MicroRNAs(miRNAs)play a significant role in axon regeneration following spinal cord injury.However,the functions of numerous miRNAs in axon regeneration within the central nervous system(CNS)remain largely unexplored.Here,we elucidate the positive role of microRNA-2184(miR-2184)in axon regeneration within zebrafish Mauthner cells(M-cells).The upregulation of miR-2184 in a single M-cell can facilitate axon regeneration,while the specific sponge-induced silencing of miR-2184 leads to impeded regeneration.We show that syt3,a downstream target of miR-2184,negatively regulates axon regeneration,and the regeneration suppression modulated by syt3 depends on its binding to Ca2+.Furthermore,pharmacological stimulation of the cAMP/PKA pathway suggests that changes in the readily releasable pool may affect axon regeneration.Our data indicate that miR-2184 promotes axon regeneration of M-cells within the CNS by modulating the downstream target syt3,providing valuable insights into potential therapeutic strategies.
基金The author was supported by grants from the Beijing Natural Science Foundation Program and the Scientifc Research Key Program of Beijing Municipal Commission of Education （KZ201510025023）, the National Natural Science Foundation of China （31471085）.
文摘Synaptotagmins （Syts） are a large family of integral membrane proteins that regulate synaptic function and membrane traffcking. Emerging evidences show involvement of Syts in human diseases. Here, we review the recent studies of several Syts （Syt1, 2, 7, 11, and 14） in the pathophysiological mechanisms of neurodegeneration disorders such as Alzheimer’s disease, Parkinson’s disease, and attention-defcit/hyperactivity disorder etc. A better understanding of the diverse physiological and pathological functions of different Syt isoforms is needed for potential therapeutic interventions in the future.
