外周血T淋巴细胞亚群、凝血指标与急性白血病患者病情的相关性研究

目的观察外周血T淋巴细胞亚群、凝血指标与急性白血病患者病情的相关性。方法选取2019年3月—2022年3月在商丘市第一人民医院收治的73例急性白血病患者作为观察组,并按1:1配比原则选择同期73名健康体检者作为对照组。比较2组T淋巴细胞亚群、凝血指标[纤维蛋白原(FIB)、D-二聚体、凝血酶原时间(PT)],并分析不同危险度分层及不同预后T淋巴细胞亚群、凝血指标,分析T淋巴细胞亚群与凝血指标相关性。结果观察组CD3^(+)、CD4^(+)、FIB均低于对照组,CD8^(+)、D-二聚体、TT均高于对照组(P<0.05);高危CD3^(+)、CD4^(+)、FIB<中危<低危,高危CD8^(+)、D-二聚体、TT>中危>低危(P<0.05);CD3^(+)、CD4^(+)与D-二聚体、TT呈负相关,与FIB呈正相关(P<0.05);CD8^(+)与D-二聚体、TT呈正相关,与FIB呈负相关(P<0.05);预后不良治疗前后CD3^(+)、CD4^(+)、FIB均低于预后良好,CD8^(+)、D-二聚体、TT均高于预后良好(P<0.05)。结论急性白血病外周血T淋巴细胞亚群、凝血指标与患者病情密切相关,可有助于临床病情监测和预后评估。

Correlation analysis of TCM syndrome types with T lymphocytes and biochemical indices in patients with HBV-related primary liver cancer

Objective:To investigate the correlation between T lymphocytes and biochemical indices in patients with Primary liver cancer(PLC)associated with hepatitis B virus(HBV)and TCM syndrome differentiation.Methods:263 HBV-related PLC patients who were admitted to the First Affiliated Hospital of Henan University of Traditional Chinese Medicine from January 2018 to December 2019 were retrospectively collected.There were 127 cases of liver depression and spleen deficiency syndrome(48.3%),48 cases of spleen deficiency and dampness syndrome(18.3%),31 cases of liver and gallbladder dampness and heat syndrome(11.8%),35 cases of liver and blood stasis syndrome(13.3%),and 22 cases of liver and kidney Yin deficiency syndrome(8.4%).The general data,T cell subsets,oncology and virology indicators,oncology characteristics,biochemical indicators and other data were counted.Epidata and Excel were used to collect and summarize the data,and SPSS26.0 software was used for statistical analysis.Results:There was no significant difference in gender and age distribution among the five syndrome types(χ^(2)=5.462,F=1.979,ALL P>0.05).The differences among T lymphocyte count(χ^(2)=57.785,P<0.001),CD4(+)T cell count(χ^(2)=47.103,P<0.001)and CD8(+)T lymphocyte count(F=12.760,P<0.001)were statistically significant.The T lymphocyte count,CD4(+)T lymphocyte count and CD8(+)T lymphocyte explicit count in patients with liver and kidney Yin deficiency syndrome were significantly lower than those in the other four syndrome types.AFP(χ^(2)=89.986,P<0.001),CEA(χ^(2)=95.501,P<0.001),CA199(χ^(2)=30.044,P<0.001)of the five syndrome types increased successively from the syndrome of liver depression and spleen deficiency to the syndrome of liver and kidney Yin deficiency,and the difference was statistically significant.There were statistically significant differences in the inner diameter of main portal vein,portal vein cancer thrombin and extrahepatic metastasis among the five syndrome types(ALL P<0.001).The main symptoms of portal vein cancer thrombin and extrahepatic metastasis were liver-gallbladder dampness-heat syndrome and liver-blood stasis syndrome.The differences among PLT(χ^(2)=39.234,P<0.001),Alb(χ^(2)=75.171,P<0.001),TBil(χ^(2)=51.140,P<0.001),AST(χ^(2)=55.881,P<0.001),PT(χ^(2)=21.515,P<0.001)were statistically significant.PLT and Alb decreased successively from the syndrome of liver depression and spleen deficiency to the syndrome of liver and kidney Yin deficiency.PLT and Alb of the syndrome of liver depression and spleen deficiency were significantly higher than those of the other four groups,and TBil and AST of the syndrome of liver and gallbladder dampness and heat were significantly higher than those of the other four groups.PT of liver and kidney Yin deficiency was significantly higher than that of the other four groups.The lymphocyte count,CD4(+) lymphocyte count and CD8(+) lymphocyte count were negatively correlated with AFP,PT and TBil(ALL P<0.05),and positively correlated with PLT(P<0.05).T lymphocyte count was positively correlated with AIb(P<0.05).Conclusion:This study found that patients with liver depression and spleen deficiency syndrome have better cellular immune function,liver function and prognosis.Patients with liver and kidney Yin deficiency have lower cellular immunity,worse liver function,and worse prognosis.Portal vein carcinoma embolus and extrahepatic metastasis were mainly characterized by dampness and heat of liver and gallbladder and blood stasis of liver.Patients with lower lymphocyte counts have poorer blood clotting,worse the liver reserve,and the higher the risk of further cancer.

Tectonic geomorphology of Türkiye and its insights into the neotectonic deformation of the Anatolian Plate

Quantitative geomorphic analyses are usually powerful in identifying active tectonics across global orogenic belts.Our present study will focus on the Anatolian Plate which hosts a lot of recent catastrophic earthquakes in Türkiye.Six geomorphic indices for 100 sub-basins around Türkiye have been computed including local relief,slope,normalized steepness index(k_(Sn)),hypsometric curve and integral(HI),transverse topographic symmetry factor(Tf),and the basin asymmetry factor(Af).The averaged kSnand Af values have shown four high-value anomalous zones,suggesting relatively high uplift rates featured by high river incision and regional tilting.The values of 0.35≤HI<0.6 for basins with S-shaped curves imply intensive tectonic activities along the eastern part of the North Anatolian Fault Zone(NAFZ),the Northeast Anatolian Fault Zone(NEAFZ),the East Anatolian Fault Zone(EAFZ),and the Central Anatolian Fault Zone(CAFZ).All results of the geomorphic indices analysis suggest a relatively high degree of tectonic activity in the following four areas,the Isparta Angle,the Eastern Black Sea Mountains,the South-eastern Anatolia Region,and the Central Anatolian fault zone.We further suggest that the eastern part of the NAFZ,NEAFZ,EAFZ,and CAFZ will be more active in tectonic activities,with a greater potential for strong earthquake occurrence.

Geomorphological responses of rivers to active tectonics along the Karahay?t Fault, Western Türkiye

Understanding the topography in active tectonic areas and assessing the rates and models of active deformation in the upper crust are primary objectives in tectonic geomorphology studies. The drainage pattern of river systems is highly sensitive to tectonically induced changes, and it often preserves the records of the formation and progression of most tectono-geomorphic processes within its boundaries. Therefore, the evolution of landforms is a consequence of the evolution of individual drainage basins in which they are formed. Assessing the rates of tectonic deformation using geomorphic data is a traditionally adopted method to characterize the nature of active faults. Globally, the Digital Elevation Model(DEM) is widely used as a crucial tool to analyze the morphotectonic features of drainage basins. In this study, some geomorphic indices were applied to investigate the impact of tectonism on landscape along the Karahay?t Fault and its associated drainage areas. These geomorphic indices are mountain front sinuosity(Smf values between 1.17-1.52), valley floor width-to-height ratio(Vf values between 0.25-1.46), basin asymmetry factor(AF values between 15-72), drainage basin shape(Bs values between 3.18-6.01), hypsometric integral and curve(HI values between 0.32-047), channel sinuosity(S values between 1-1.6), normalized steepness index(Ksn values between 1-390) and Chi integral(χ values between 200-4400). The development of drainage areas on the hanging wall and footwall block of the Karahayit Fault differs depending on the uplift. The drainage areas developed on the hanging wall present different patterns depending on the regional uplift caused by the fault. This reveals that the fault contributed significantly to the development of drainage areas and regional uplift in the region. In addition, the maximum earthquake magnitude that may occur in the future on the Karahayit Fault, whose activity is supported by geomorphic indices, is calculated as 6.23. Since an earthquake of this magnitude may cause loss of life and property in the region, precautions should be taken.

RSSI-Based 3D Wireless Sensor Node Localization Using Hybrid T Cell Immune and Lotus Optimization

Wireless Sensor Network(WSNs)consists of a group of nodes that analyze the information from surrounding regions.The sensor nodes are responsible for accumulating and exchanging information.Generally,node local-ization is the process of identifying the target node’s location.In this research work,a Received Signal Strength Indicator(RSSI)-based optimal node localization approach is proposed to solve the complexities in the conventional node localization models.Initially,the RSSI value is identified using the Deep Neural Network(DNN).The RSSI is conceded as the range-based method and it does not require special hardware for the node localization process,also it consumes a very minimal amount of cost for localizing the nodes in 3D WSN.The position of the anchor nodes is fixed for detecting the location of the target.Further,the optimal position of the target node is identified using Hybrid T cell Immune with Lotus Effect Optimization algorithm(HTCI-LEO).During the node localization process,the average localization error is minimized,which is the objective of the optimal node localization.In the regular and irregular surfaces,this hybrid algorithm effectively performs the localization process.The suggested hybrid algorithm converges very fast in the three-dimensional(3D)environment.The accuracy of the proposed node localization process is 94.25%.

两孔法腹腔镜结直肠癌根治术对结直肠癌患者血清应激指标及T淋巴细胞亚群的影响

目的:探讨两孔法腹腔镜结直肠癌根治术(LRRC)在结直肠癌中的疗效。方法:回顾性分析2021年1月—2024年1月江西省中西医结合医院收治的84例结直肠癌患者临床资料,按手术方法不同分组,将接受常规多孔LRRC的42例设为对照组,将接受两孔法LRRC的42例设为观察组。比较两组手术情况、血清应激指标、T淋巴细胞亚群、肿瘤标志物、并发症。结果:观察组首次下床活动时间、首次排气时间及术后住院时间分别为(26.88±2.42)h、(40.25±4.35)h、(8.41±1.28)d,短于对照组的(34.68±3.57)h、(51.74±5.89)h、(11.05±1.34)d,差异均有统计学意义(P<0.05);观察组术后皮质醇、促肾上腺皮质激素、去甲肾上腺素水平分别为(759.65±55.82)nmol/L、(19.55±1.54)pmol/L、(87.14±5.59)μg/L,低于对照组的(824.28±66.07)nmol/L、(22.06±2.42)pmol/L、(99.52±6.83)μg/L,差异均有统计学意义(P<0.05);观察组术后CD4^(+)、CD4^(+)/CD8^(+)为(35.41±3.25)%、(1.19±0.21),高于对照组的(31.84±3.19)%、(1.00±0.19),CD8^(+)为(29.86±2.57)%,低于对照组的(31.89±2.87)%,差异均有统计学意义(P<0.05);两组术后肿瘤标志物水平、并发症发生率相比,差异均无统计学意义(P>0.05)。结论:两孔法LRRC治疗结直肠癌效果更佳,可降低手术应激反应,缩短术后下床活动、排气时间等,减轻创伤引起的免疫抑制,且安全性尚可。

超声心动图心室重构指标与心电图QRS-T夹角在HFpEF患者中的检测意义

目的:探究超声心动图心室重构指标与心电图QRS-T夹角在射血分数保留的心力衰竭(heart failure with preserved ejection fraction,HFpEF)患者中的检测意义。方法:选择2021年1月至2023年12月收治的62例HFpEF患者进行研究,根据左心室重构发生情况分为重构组(n=21)和未重构组(n=41),选择同期体检的正常人员30例为对照组,均行常规超声心动图检查、十二导联心电图检查。超声心动图检测各组心脏功能和心室重构指标,十二导联心电图测量心电图QRS-T夹角,分析心室重构指标与心电图QRS-T夹角的相关性。结果:重构组左心射血分数、左室短轴缩短率和左室每搏量低于未重构组与对照组(P<0.05),未重构组低于对照组(P<0.05);重构组、未重构组与对照组二尖瓣血流频谱E峰与A峰之比、左室重构指数和平均左室周径向心缩短率水平依次降低(P<0.05),左室质量指数水平依次升高(P<0.05);重构组、未重构组与对照组T轴、QRS-T夹角依次降低(P<0.05),QRS轴依次增大(P<0.05);相关性分析显示,患者超声心动图心室重构指标E/A和LVRI与心电图QRS-T夹角呈高度负相关(P<0.05),MVCF和LVMI与心电图QRS-T夹角呈正相关(P<0.05)。结论:心电图QRS-T夹角与HFpEF患者超声心动图心室重构指标有一定相关性,具有一定临床意义。

Method for pedestrian signal timing at signalized intersections

The pedestrian timing at signalized intersections is studied aiming at the problems of the inconsistency of the vehicular and pedestrian timing requirements and the insufficiency of pedestrian clearance. Based on the formulae of WALK and flashing DON＇T WALK （FDW） in the highway capacity manual （HCM）, the relationship between pedestrian signal indications and vehicular signal indications is discussed using the theory of traffic flow. Then, methods of pedestrian timing for different cases are established, particularly the methods of the pedestrian green adjustment. Ways of pedestrian crossing are analyzed for roadways with different forms and widths of the median island. The sampling values of calculation parameters are studied, and the recommended formulae of pedestrian timing for different conditions are presented.

腹腔镜下腹股沟疝修补术对手术效果及T淋巴细胞百分比的影响

目的探讨腹腔镜下腹股沟疝修补术对手术效果及T淋巴细胞百分比的影响。方法选取2017年1月-2019年1月腹股沟疝患者280例作为研究对象,随机分为对照组(n=140)和观察组(n=140)。对照组采用开放无张力疝修补术,观察组采用腹腔镜下无张力疝修补术,术后7 d对患者治疗效果进行评估,比较两组手术指标、疼痛视觉模拟评分(VAS)、T淋巴细胞百分比及术后并发症。结果观察组与对照组手术时间比较,差异无统计学意义(P>0.05);观察组出血量、术后自主活动时间及VAS评分少于对照组(P<0.05);观察组治疗费用多于对照组(P<0.05);观察组手术后7 d CD3^+、CD4^+和CD4^+/CD8^+百分比高于对照组(P<0.05);观察组手术后7 d CD8^+百分比低于对照组(P<0.05);观察组手术后切口疼痛、尿潴留、阴囊水肿及切口感染发生率与对照组比较,差异均无统计学意义(P>0.05)。结论将腹腔镜手术用于腹股沟疝患者中,手术创伤较小,能改善患者T淋巴细胞百分比,未增加术后并发症发生率,值得推广应用。

HLA-A^＊0201限制性CTL表位肽的三维定量构效关系的研究

运用比较分子力场 (CoMFA)和比较分子相似性指数分析 (CoMSIA)方法研究了 5 0个HLA A 0 2 0 1限制性CTL表位九肽结构与亲和性间的关系 ,另外 15个表位九肽作为预测集用于检验模型的预测能力 .结果表明采用CoMSIA得到的构效关系模型 (q2 =0 .62 8,r2 =0 .997,F =840 .419)要明显优于采用CoMFA得到的构效关系模型 .在CoMSIA计算中 ,当引入疏水场时 ,三维构效关系模型得到明显改善 ,通过该三维构效关系模型 ,可较精确地估算预测集中 15个CTL表位肽与HLA A 0 2 0 1间的亲和力 (r2pred=0 .743 ) .通过分析分子场等值面图在空间的分布 ,可以观察到表位肽分子周围的立体及疏水特征对表位肽与HLA A 0 2 0 1间结合亲和力的影响 ,从而为进一步对CTL表位肽进行结构改造并基于此进行治疗性疫苗分子设计提供理论基础 .

手工MGIT液体培养结核分枝杆菌污染再处理分析

目的分析手工分枝杆菌培养管(MGIT)液体培养法检测结核分枝杆菌中的污染再处理效果。方法收集疑似新发肺结核初诊的1 088例患者的痰标本,所有标本均分别用液体MGIT法、固体改良罗氏培养基L-J法及痰涂片抗酸染色进行检测,统计MGIT法首次培养总污染率和去污染后再次培养的总污染率,并和固体L-J法污染率进行比较。结果 MGIT法的首次污染率涂片阳性(简称涂阳)组为11.24%,涂片阴性(简称涂阴)组为7.29%,总污染率为7.90%;L-J法涂阳组的污染率为2.96%,涂阴组为3.80%,总污染率为3.68%,两者之间差异有统计学意义(P均<0.05)。去污染后涂阳组的再污染率为0.59%,涂阴组为4.68%,总污染率为4.04%,与L-J法相应各组间差异无统计学意义(P均>0.05);去污染后再培养涂阳组污染率低于涂阴组(P<0.05)。结论 MGIT法可去污染再培养,以降低污染率,特别是涂阴标本应调整和增加前处理的消化时间或灭菌剂的浓度,以控制首次MGIT法的污染率,减少再处理去污染次数。建议临床医生应在控制其他细菌感染后再留标本做结核分枝杆菌培养。

基于T-S网络的建筑施工安全预警模型研究

建筑施工安全问题日益凸显,迫切需要进行建筑施工安全预警模型方面的研究。本文结合以往的建筑施工安全事故案例,从人、物、环境、管理四个方面,对建筑施工安全影响因素进行分析,构建建筑施工安全预警指标体系。本文将建筑施工安全预警状态分为5个等级,并采用专家打分法对各预警区间定量划分,并借鉴模糊神经网络预警模型在其他学科领域的应用,建立了基于T-S网络的建筑施工安全预警模型。然后经过大量样本的训练和检测,使网络具有良好的泛化性。本文选取沈阳市某3个在建项目进行预警,发现与传统预警模型得出的结果一致,进一步验证了T-S网络预警模型的准确性和可行性。最终,得出T-S网络预警模型在建筑施工安全预警中具有适用性的结论。

自身免疫性肝炎患者外周血T细胞PD-1的表达及与肝功能指标的相关性

目的研究自身免疫性肝炎(AIH)患者外周血CD4+、CD8+T细胞表面程序性死亡受体1(PD-1)的表达,并探讨其与肝功能指标的相关性。方法收集43例AIH患者(包括活动期31例和缓解期12例),并选择18例健康体检者为对照组。采用流式细胞术检测外周血CD4+、CD8+T细胞表达PD-1的细胞百分比。结果 AIH组外周血CD4+、CD8+T细胞表达PD-1的细胞百分比(分别为7.36±4.45%、6.71±3.84%)显著高于对照组(分别为3.78±2.32%、3.47±2.18%)(P均<0.05);AIH活动期组外周血CD4+、CD8+T细胞表达PD-1的细胞百分比(分别为8.73±6.81%、7.44±2.75%)明显高于缓解期组(分别为5.42±2.14%、4.82±2.04%)和对照组(分别为3.78±2.32%、3.47±2.18%)(P均<0.05),且缓解期组高于对照组(P<0.05)。AIH患者外周血CD4+、CD8+T细胞PD-1表达与TBIL、ALT、AST及Ig G水平均成正相关关系(r分别为0.781、0.887、0.825、0.893,P均<0.05)。结论 AIH患者外周血CD4+、CD8+T细胞表面PD-1表达上调,且与肝功能指标成正相关,提示PD-1可能在AIH的发生及发展中发挥重要作用。

28例胆总管探查术后不放置T管临床分析

目的探讨胆总管探查术后不放置T管的可行性和适应证。方法对28例胆总管探查术后不放置T管患者的临床资料进行回顾性分析。结果全部患者均顺利康复,无胆漏。平均住院时间10d。住院费用减少。随访1年,无胆管狭窄表现。结论适应证:①术中证实胆道无结石残留;②胆管壁无明显炎性反应水肿;③胆总管内径>0·8cm;④胆总管下端通畅。掌握好适应证,胆总管探查术后不放置T管行一期缝合是安全可行性的。不失为肝胆外科医师的良好选择。

滴定分析中固、液体鉻黑T指示剂应用比较

分别采用初配和配制7天后的固、液体鉻黑T指示剂标定EDTA溶液及测定水的总硬度,根据实验结果分析两种形态指示剂的使用效果,结果表明,新配制的EBT固、液指示剂,标定EDTA时有显著性差异,测定水的总硬度时,两种指示剂无显著性差异;配制7天后的EBT固、液指示剂,标定EDTA及测定水的总硬度时,使用两种指示剂均存在显著性差异.经F检验,液体鉻黑T指示剂放置7天后测定精密度低于新配制指示剂,固体鉻黑T指示剂放置前后测定精密度无显著变化.因此得出结论,若实验时新配制指示剂,选用液态为佳,否则,要使用固体鉻黑T指示剂.

铬黑T指示剂稳定体系的研究及应用

对EDTA络合滴定法测定硬度的铬黑T(EBT)指示剂进行了研究.通过铬黑T溶液吸光性的测定,对铬黑T各种液体体系的溶解性、稳定性进行了探讨,找到了0.1%EBT-20%NH3-NH4Cl-0.1%PVP乙醇溶液的稳定体系,并将其应用于自来水中总硬度的测定,终点颜色变色敏锐,平行测定结果好,精密度高.并与固体指示剂进行了对比.

T淋巴细胞占比及DD/Fib与急性髓系白血病患者临床病理指标和预后的关系

目的探讨T淋巴细胞占比及DD/Fib与急性髓系白血病患者临床病理指标和预后的关系。方法选取190例AML患者作为研究组,同时选取80例同期体检的健康人员作为对照组,比较2组患者的T淋巴细胞占比及DD/Fib等差异。结果除CD8^(+)外,研究组的CD4^(+)、CD4^(+)/CD8^(+)均低于对照组,而DD/Fib则显著高于对照组(P<0.05)。研究组中完全缓解组、未缓解组及难治复发组CD8^(+)指标比较无差异(P>0.05),而CD4^(+)、CD4^(+)/CD8^(+)逐渐降低,DD/Fib则逐渐升高(P<0.05)。死亡组CD4^(+)、CD4^(+)/CD8^(+)均低于生存组,而DD/Fib则显著高于生存组(P<0.05);髓外浸润、CD4^(+)、CD4^(+)/CD8^(+)及DD/Fib均为影响AML患者预后不良的独立危险因素(P<0.05)。结论随着AML病情进展,其T淋巴细胞亚群逐渐受到抑制,且高凝及纤溶亢进状态愈发明显,同时可在一定程度上反映患者的预后情况,且发现髓外浸润、CD4^(+)、CD4^(+)/CD8^(+)及DD/Fib均为影响AML患者预后不良的独立危险因素。

铬黑T(EBT)失效分析

针对铬黑T(EBT)在使用中发生终点显色异常,影响测量结果准确率的现状,运用可靠性技术对EBT失效进行原因分析。通过分析失效原因,提出针对性措施并实施;在已有国家标准和行业标准配置的基础上,摒弃显色不灵敏、用量大的盐酸羟胺为介质的配置方式;进一步明确国家标准和行业标准中尚无使用有效期限的固体指示剂和三乙醇胺为介质的液体指示液;调整介质配比、明确加入量及贮存条件等。通过改进措施验证,铬黑T(EBT)终点显色失效问题已解决,达到提高铬黑T(EBT)使用可靠性的目的。

图K_1×m∪t=1C_((2n)_t)的边优美指标集和超边优美标号

对于非负整数k,讨论了图K1×m∪t=1C2nt为k-边优美图的必要条件,给出了构造该图k-边优美的一般步骤,进而利用递归方法构造k-边优美图标号并给出详细证明,从而完全解决了图K1×m∪t=1C2nt的边优美指标集问题。最后,证明了该图是超边优美图。

基于动态T检验的修正最小二乘BFGS定位算法

针对传统基于接收信号强度指示RSSI(Received Sgnal Strength Indicator)的最小二乘定位算法精度有限的问题，从降低信号噪声影响出发，提出一种基于动态T检验的修正最小二乘-BFGS定位算法。在测距阶段，将T检验法动态应用于RSSI值筛选，以消除较大测量误差影响，提高测距精度;定位阶段，先基于平均测算距离对传统的最小二乘法进行修正，提升初步定位精度0.3~2米;在此基础上，以信号强度误差最小为目标优化函数，基于BFGS算法对修正最小二乘法计算的初值进行迭代求精，以进一步提高定位精度。仿真实验表明，该算法有效提升了定位精度，在100mxlOOm范围内可比传统最小二乘定位误差降低4~11米。