Glucocorticoid(GC) steroid hormones are used to treat acute lymphoblastic leukemia(ALL) because of their pro-apoptotic effects in hematopoietic cells.However,not all leukemia cells are sensitive to GC,and no assay to ...Glucocorticoid(GC) steroid hormones are used to treat acute lymphoblastic leukemia(ALL) because of their pro-apoptotic effects in hematopoietic cells.However,not all leukemia cells are sensitive to GC,and no assay to stratify patients is available.In the GC-sensitive T-cell ALL cell line CEM-C7,auto-up-regulation of RNA transcripts for the glucocorticoid receptor(GR) correlates with increased apoptotic response.This study aimed to determine if a facile assay of GR transcript levels might be promising for stratifying ALL patients into hormone-sensitive and hormone-resistant populations.The GR transcript profiles of various lymphoid cell lines and 4 bone marrow samples from patients with T-cell ALL were analyzed using both an optimized branched DNA(bDNA) assay and a real-time quantitative reverse transcription-polymerase chain reaction assay.There were significant correlations between both assay platforms when measuring total GR(exon 5/6) transcripts in various cell lines and patient samples,but not for a probe set that detects a specific,low abundance GR transcript(exon 1A3).Our results suggest that the bDNA platform is reproducible and precise when measuring total GR transcripts and,with further development,may ultimately offer a simple clinical assay to aid in the prediction of GC-sensitivity in ALL patients.展开更多
针对传统的高低阻抗微带低通滤波器和开路端短截线微带低通滤波器体积较大、过渡带较缓且插入损耗较大的问题,采用高阻抗传输线单元加载并联倒T形枝节方法,构成带阻滤波支路,抑制了寄生通带,减小了滤波器面积。以7阶切比雪夫低通滤波器...针对传统的高低阻抗微带低通滤波器和开路端短截线微带低通滤波器体积较大、过渡带较缓且插入损耗较大的问题,采用高阻抗传输线单元加载并联倒T形枝节方法,构成带阻滤波支路,抑制了寄生通带,减小了滤波器面积。以7阶切比雪夫低通滤波器为例进行了设计和测试。实验结果表明,倒T形并联枝节滤波器截止频率为5.06 GHz,通带内最大插入损耗小于0.95 d B,在5.61～13.6 GHz内阻带抑制超过21 d B,而滤波器的面积比传统的开路端短截线微带低通滤波器减小20%。展开更多
基金supported in part by a grant from NCI (No.CA116042) to W.V.Vedeckis
文摘Glucocorticoid(GC) steroid hormones are used to treat acute lymphoblastic leukemia(ALL) because of their pro-apoptotic effects in hematopoietic cells.However,not all leukemia cells are sensitive to GC,and no assay to stratify patients is available.In the GC-sensitive T-cell ALL cell line CEM-C7,auto-up-regulation of RNA transcripts for the glucocorticoid receptor(GR) correlates with increased apoptotic response.This study aimed to determine if a facile assay of GR transcript levels might be promising for stratifying ALL patients into hormone-sensitive and hormone-resistant populations.The GR transcript profiles of various lymphoid cell lines and 4 bone marrow samples from patients with T-cell ALL were analyzed using both an optimized branched DNA(bDNA) assay and a real-time quantitative reverse transcription-polymerase chain reaction assay.There were significant correlations between both assay platforms when measuring total GR(exon 5/6) transcripts in various cell lines and patient samples,but not for a probe set that detects a specific,low abundance GR transcript(exon 1A3).Our results suggest that the bDNA platform is reproducible and precise when measuring total GR transcripts and,with further development,may ultimately offer a simple clinical assay to aid in the prediction of GC-sensitivity in ALL patients.
文摘针对传统的高低阻抗微带低通滤波器和开路端短截线微带低通滤波器体积较大、过渡带较缓且插入损耗较大的问题,采用高阻抗传输线单元加载并联倒T形枝节方法,构成带阻滤波支路,抑制了寄生通带,减小了滤波器面积。以7阶切比雪夫低通滤波器为例进行了设计和测试。实验结果表明,倒T形并联枝节滤波器截止频率为5.06 GHz,通带内最大插入损耗小于0.95 d B,在5.61～13.6 GHz内阻带抑制超过21 d B,而滤波器的面积比传统的开路端短截线微带低通滤波器减小20%。
