Relationship of serum neurotransmitters with anxiety depression, T lymphocyte subsets and NK cells in patients with lung cancer chemotherapy

Objective:To study the relationship of serum neurotransmitters with anxiety depression, T lymphocyte subsets and NK cells in patients with lung cancer chemotherapy.Methods: 56 cases of patients with advanced lung cancer who received chemotherapy in the First Affiliated Hospital of Chengdu Medical College between July 2013 and August 2016 were collected as observation group, and 50 healthy subjects who received physical examination in our hospital during the same period were selected as normal control group. Serum neurotransmitter, negative emotions and immune index levels were compared between the two groups of subjects. Pearson test was used to evaluate the relationship of serum neurotransmitter contents with negative emotions and immune index levels in patients with lung cancer chemotherapy. Results: Serum neurotransmitters DA, 5-HT and NE contents in observation group were lower than those in normal control group;SAS and SDS scores were higher than those of normal control group;peripheral blood CD4+T lymphocyte level, CD4+/CD8+ ratio and NK cell level were lower than those in normal control group while CD8+ T lymphocyte level was higher than that in normal control group. Pearson test showed that serum neurotransmitters DA, 5-HT and NE contents in patients with lung cancer chemotherapy were directly correlated with anxiety depression, T lymphocyte subset and NK cell levels.Conclusion: Serum neurotransmitter expression decrease in patients with lung cancer chemotherapy, and this is one of the important causes of anxiety depression and immune dysfunction in patients.

Study on T lymphocyte subsets and NK cells in patients with Graves' disease combined with type 2 diabetes

Objective To investigate changes in T lymphocyte subsets and NK cells in patients with simple Graves’ disease(GD)and Graves’ disease combined with type 2 diabetes mellitus(GD/T2DM).Methods Fifteen cases of GD/T2DM were selected from our hospital from November 2001 to November 2004.Before and after therapy thyroid function,thyroglobulin antibody(TGA),thyroid microsomal antibody(TMA)and blood glucose level were measured,and T lymphocyte subsets(CD3,CD4,CD8,CD4/CD8)and NK cells(CD56)were measured by immunofluorescence double labeling monoclonal antibody and flow cytometry,respectively.At the same time,comparison was made with simple GD(15 cases),T2DM(15 cases)and healthy control(20 cases).Results Before therapy,CD4/CD8,CD4 and NK cells in GD/T2DM were less than normal,and there was no significant difference in comparison with simple GD(P<0.05).In T2DM group,only CD4/CD8 and CD4 were less than those of healthy controls(P<0.05).When thyroid function recovered after 1 to 3 months of methimazole treatment in both GD/T2DM and simple GD groups,various indexes recovered,which were more obvious in simple GD.Conclusion Immune hypofunction of GD may be the key to the immune abnormality of GD/T2DM,which is more significant than that of simple GD or T2DM.The recovery of thyroid function and immune abnormality is not consistent,and the recovery of GD is more significant than that of GD/T2DM.

鼻型结外NK/T细胞淋巴瘤误诊为鼻窦炎眶周蜂窝织炎1例

1临床资料患者,男,34岁,因“鼻堵脓涕1月余伴右眼红肿2周”于门诊就诊。患者1个月前在劳累饮酒后出现双侧持续性鼻堵、黄脓涕,伴双侧额部头痛、右眼眶重度胀痛,需口服止痛药缓解疼痛。2周前右眼疼痛加重,伴视物稍模糊,无复视,伴发热,体温最高38.2℃。门诊初步疑诊“急性鼻窦炎眶周蜂窝织炎”收治入院。发病以来患者神清,精神可,食欲尚可,近6个月体重下降约30斤,夜间睡眠差,伴乏力。

Distribution of natural killer cells and T-lymphocyte subsets in peripheral blood,gallbladder cancer and surrounding tissue

BACKGROUND: The patient with malignant tumor always show immunologic function drawback and ingravescent with tumor development, especially in the aspect of cell-mediated immunity. This study was undertaken to define the relationship between the immune function of local cells and cancer development by investigating the distribution of natural killer (NK) cells and T-lymphocyte subsets in peripheral blood, the cancer tissue and the tissue surrounding gallbladder carcinoma. METHODS: The numbers of CD4(+) and CD8(+) T-lymphocytes and NK cells were measured by flow cytometry in samples taken from gallbladder cancer tissue, the surrounding tissues and peripheral blood of 38 patients, and compared with the numbers in the peripheral blood and gallbladder tissue of 30 patients with cholecystitis as controls. RESULTS: The numbers of CD4(+) and CD8(+) T-cells and NK cells in gallbladder cancer tissues were significantly higher than those in the surrounding tissue and gallbladder with gallstone. However, the ratio of CD4(+)/CD8(+) was lower in the cancer tissue than that in the surrounding tissue and tissue from gallbladders with gallstones. The distribution of CD4(+) and CD8(+) T-cells and NK cells in mucous membrane of cholecystitis gallbladder and that in the tissue surrounding gallbladder cancer were significantly different. CONCLUSIONS: Disproportionate and imbalanced distribution of NK cells and subsets of T-lymphocytes occurs in the mucous membrane proper of gallbladder cancer and surrounding tissue. Although gallbladder cancer tissue has higher expressions of CD4(+), CD8(+) and NK cells, the immune function is low or in an inhibited state. In gallbladder cancer immunization therapy, local cellular immunological function should be enhanced and the protective barrier improved.

Effects of Intraoperative Administration of Dexmedetomidine on the Percentage of T-Lymphocyte Subsets and Natural Killer Cells in Patients with Colorectal Cancer

Study Objective: To observe the effect of dexmedetomidine (DEX) on T-lymphocyte subsets and natural killer (NK) cells in the peripheral blood of perioperative patients with colorectal cancer. Design: A random double-blind control clinical study. Setting: A university hospital. Patients: Forty patients with colorectal cancer, ASA I-П. Interventions: All patients were randomly divided into a DEX group (n = 20) and a control group (n = 20). Before induction of anesthesia, epidural catheters were placed in the L1-L2 or T12-L1 intervertebral spaces. The DEX group received 1 μg/kg of DEX (200 μg/50 ml) intravenously for 15 min prior to the surgery, which was then infused at a rate of 0.5 μg/kg/h until 30 min before the end of the surgery. The control group received an intravenous infusion of saline (50 ml) instead of DEX during the same periods as the DEX group. All patients received routine anesthesia and postoperative analgesia. Measurements: Blood samples from all patients were collected at the following time points: before anesthesia (T0), 24 h after surgery (T1), 48 h after surgery (T2) and 72 h after surgery (T3). Changes in T-lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) and NK cells were determined by flow cytometry. Main Results: Compared with the control group, the percentages of CD3+ and CD4+ cells and the CD4+/CD8+ ratio in the DEX group increased significantly from T1 to T3

异常表达CD20的结外NK/T细胞淋巴瘤病理特点分析并文献复习

目的探索异常表达CD20的结外NK/T细胞淋巴瘤的病理形态特点、诊断要点及鉴别诊断。方法分析3例异常表达CD20的结外NK/T细胞淋巴瘤,结合病理形态特点、免疫表型及与其他形态学特点和免疫表型类似的淋巴瘤的鉴别诊断,并进行文献复习。结果异常表达CD20的结外NK/T细胞淋巴瘤形态学特点与普通型结外NK/T细胞淋巴瘤类似,免疫表型表现为部分T细胞标记阳性、CD56、细胞毒标记物、EBER ISH阳性、CD20弱-中等阳性,其余B细胞标记物CD79a、PAX-5均为阴性。其中2例肿瘤细胞阳性表达CD8。结论异常表达CD20的结外NK/T细胞淋巴瘤是一种罕见免疫表型的NK细胞源性淋巴瘤,需要综合分析抗体表达情况及和其他淋巴瘤进行鉴别诊断,免疫组织化学染色阳性模式以及多抗体联合应用对于明确诊断有重要作用。

PD-1/PD-L1抑制剂治疗结外NK/T细胞淋巴瘤研究进展

结外NK/T细胞淋巴瘤(extranodal NK/T-cell lymphoma,ENKTCL)是一种罕见的且具有高度侵袭性的非霍奇金淋巴瘤,与EB病毒感染密切相关。目前,ENKTCL治疗手段包含化疗、放疗、造血干细胞移植以及免疫治疗等。近年来,随着ENKTCL发病机制的深入研究,针对PD-1/PD-L1的免疫检查点治疗被认为具有重要的临床价值。本文综述了PD-1/PD-L1抑制剂的作用机制,总结了其在ENKTCL治疗领域的临床研究进展以及预测疗效的标志物的选择,并概述了PD-1/PD-L1抑制剂治疗ENKTCL所面临的限制因素以及未来的发展前景。

Effect on T cell subsets and function of isletβ cells of levemir combined with acarbose in elder patients with early-onset type 2 Diabetes Mellitus

Objective:To discuss the effect of the combined therapy of levemir and acarbose on T cell subsets and function of isletβ cells in elder patients with early-onset type 2 Diabetes Mellitus. Methods:According to the number parity of entry sequence, 100 cases of elder patients with early-onset type 2 Diabetes Mellitus are divided into the control group and the observation group of 50 cases. The control group was treated with novolin and acarbose, the observation group was given subcutaneous injection of levemir and acarbose treatment. Compare the T cell subsets and function of isletβ cells in two group of patients before the treatment (T0), treatment for 4 weeks (T1) ,treatment for 8 weeks (T2).Results:(1) The levels of T0, T1, T2CD3+, CD4+, CD4+/CD8+ were increased in both groups, and CD8+ decreased. Among them, the levels of T1, T2CD3+, CD4+, CD4+/CD8+ of the observation group were obviously higher than the control group, the level of CD8+ was lowly than the control group, the difference was statistically significant;(2) In the stage of T0, T1, T2, the levels of FPG, HbA1c, HOMA-IR were showed a downward trend, the levels of FIns, HOMA-B were increased. In these two groups, the levels of T1, T2FPG, HbA1c, HOMA-IR of the observation group were lower than the control group, and the levels of FIns, HOMA-B were higher than the control group, the difference was statistically significant;(3) In the control group occurred 3 cases of hypoglycemia, and the incidence of adverse reactions was 6%. However, in the observation group no occurred adverse reactions, the difference was statistically significant.Conclusions:The combined therapy of levemir and acarbose in elder patients with early-onset type 2 Diabetes Mellitus, It helps to improve immune function, protect the isletβ-cell function.

Detection of lymphocyte subsets and regulatory T cells in peripheral blood of patients with acute leukemia and its clinical significance

Objective To study the changes of lymphocyte subsets and regulatory T cells in peripheral blood of patients with acute leukemia(AL) and its clinical significance.Methods The different levels of peripheral blood lymphocyte subsets and regulatory T cells of 60 AL patients and 40 normal controls were detected with flow cytometry.Results Compared with the normal controls,the percentages of CD3+ T cells,CD4+ T cells,CD16+CD56+ NK cells and the ratio of CD4+ /CD8+ obviously decreased in newly diagnosed AL group(P <0.05),while their percentages of CD8+ T cells and CD19+ B cells significantly increased(P <0.01).The percentage of CD4+ T cells and the ratio of CD4+ /CD8+ in acute lymphoblastic leukemia(ALL) group were much lower than those in acute myelogenous leukemia(AML) group(P <0.01).Compared with these in control group,the proportions of CD4+ CD25high Treg cells and CD4+ CD25+ T cells in newly diagnosed AL group were significantly increased(P <0.01).Conclusion Cellular immune function is significantly abnormal in patients with AL.Compared with AML patients,ALL patients had poorer cellular immune function.The increased CD4 + CD25high Treg cells might be one of the important reasons of immunosuppression in AL.Detection of lymphocyte subsets and regulatory T cells is of clinical value on the evaluation of therapeutic effect and prognosis in AL patients.

误诊为丹毒的结外NK/T细胞淋巴瘤一例

结外NK/T细胞淋巴瘤(ENKTL)是一种罕见的侵袭性非霍奇金淋巴瘤亚型。本文报道以面部肿胀为表现的结外NKT细胞淋巴瘤一例,患者曾多次被误诊为“丹毒、蜂窝组织炎”等,最终通过病理、免疫组化等检查确诊,给予DDGP方案(培门冬酶、吉西他滨、顺铂、地塞米松)化疗,随访9个月患者恢复良好。

^(18)F-FDG PET/CT影像组学预测结外NK/T细胞淋巴瘤预后的价值

目的探讨^(18)F-FDG PET/CT影像组学模型对结外NK/T细胞淋巴瘤(ENKTL)无事件生存期的预测价值。资料与方法回顾性收集2013年1月—2021年1月于河南省人民医院治疗前行^(18)F-FDG PET/CT检查的ENKTL患者90例,随机分为训练组63例和验证组27例。从基线PET和CT图像上提取特征,应用最小绝对收缩和选择算子算法结合Cox生存分析筛选特征并构建临床模型、影像组学模型和临床+影像组学复合模型,以模型风险评分的中位数作为截断值将患者分为高危组和低危组。使用C指数和受试者工作特征曲线评价3个模型的预测性能。基于最优模型构建列线图,采用校准曲线描述最优模型预测ENKTL患者生存概率与实际概率的一致性,使用Kaplan-Meier分析和对数秩检验评估最优模型的预后价值。结果复合模型在训练组(C指数0.791,95%CI 0.702~0.879,曲线下面积为0.882)和验证组(C指数0.770,95%CI 0.650~0.889,曲线下面积为0.720)中比单独的临床模型和影像组学模型的预后表现更好。校准曲线表明复合模型在预测3年无事件生存期概率与实际结果的一致性较好,生存曲线显示高危组的无事件生存期显著低于低危组。结论基于影像组学和临床参数构建的复合模型可以提供更全面的预后信息并提高诊断准确性。列线图为ENKTL患者的风险分层提供了一种无创的诊断工具,有助于个体化治疗。

Differential Expressions of Selected Activating and Inhibitory Receptors on K562-Stimulated Natural Killer (NK) Cells in HIV-1 and HIV-2 Infections

Context: The functional activity of NK cells depends on the balance between the engagement of activating and inhibitory receptors on the cell surface with their ligands, which enables them to kill infected cells. Objectives: The aim of this study was to evaluate and compare expressions of selected activating and inhibitory receptors on stimulated NK cells in HIV-1 and HIV-2 infections. Methods: PBMCs were analysed for activating (NKp30, NKp44, NKp46) and inhibitory (CD158a, CD158b, p70) receptor expressions in 30 HIV-1, 30 HIV-2 and 30 HIV uninfected healthy control (HC) subjects by flow cytometry after stimulating with K562 cells. Results: There was an expression of other receptors following an already in vitro engagement of NK cells with K562 cells. Higher expression of the activating receptors, NKp44 (p = 0.029) and NKp46 (p = 0.032) on NK cells from HIV-2 compared to HIV-1 infected individuals but similar NKp30 expression (p = 0.980). The levels of expression of inhibitory receptor CD158a were similar between HIV-1 and HIV-2 infected subjects (p = 0.309) but there was significant up-regulation of inhibitory receptors p70 (p = 0.010) and CD158b (p = 0.05) in HIV-1 compared to HIV-2 subjects. Conclusion: Despite the in vitro engagement of NK cells with stimulating K562 cells, our data showed differential expressions of other selected activating and inhibitory receptors in HIV-1 and HIV-2 infected subjects.

以口腔黏膜溃疡为首发表现的结外鼻型NK/T细胞淋巴瘤的多学科诊疗1例

结外鼻型NK/T细胞淋巴瘤(extranodal nasal type NK/T-cell lymphoma,ENKTL)是一种以破坏面部中份结构为主的NK/T细胞来源的非霍奇金淋巴瘤,临床少见。以口腔黏膜溃疡为首发症状的病例罕见且易与其他疾病相混淆,在诊断上极其困难。本文报道1例以颊黏膜及牙龈溃疡为首发表现的ENKTL的多学科诊疗,并分析该疾病的临床病理学特征、诊断、鉴别诊断和治疗,以期为临床诊治相关病例提供参考。

Effect of Mg^(2+)level on the functions of CD8^(+)T lymphocytes and NK cells in patients with COVID-19

Objective:To investigate the changes of Mg^(2+) levels in serum and peripheral blood mononuclear cells(PBMCs)of patients with COVID-19 and its effects on the functions of CD8^(+)T lymphocytes and NK cells.Methods:A total of 165 COVID-19 patients hospitalized in Ezhou Central Hospital from January 20 to February 20,2020 were divided into mild/common group(98 cases)and severe/critical group(67 cases).At the same time,34 healthy persons were selected as the control group.Peripheral blood was collected and PBMCs were isolated,the level of Mg^(2+) in serum and PBMCs was detected.The subsets of CD8^(+)T lymphocytes and NK cell and the expression levels of their surface inhibitory molecular PD-1 and activator molecular NKG2D were detected by flow cytometry.The correlation between Mg^(2+) concentration and the expression levels of PD-1 and NKG2D was also analyzed.Results:Compared with the control group,the concentration of Mg^(2+) in serum and PBMCs,the counts of CD8^(+)T lymphocytes and NK cell in patients with mild/common and severe/critical groups were significantly reduced(P<0.05),while the expression level of surface inhibitory molecular PD-1 were significantly increased(P<0.05),while the expression level of the activation molecule NKG2D were significantly decreased(P<0.05).However,the changes of the above indicators in patients with severe/critical group were greater than those in the mild/common group(P<0.05).In addition,the Mg^(2+) concentration in COVID-19 patients was negatively correlated with the expression level of PD-1 on CD8^(+)T lymphocytes and NK cells(P<0.05),and positively correlated with the expression levels of NKG2D(P<0.05).Conclusion:The concentration of Mg^(2+) in the serum and PBMCs of COVID-19 patients is significantly reduced,which may cause the function of CD8^(+)T lymphocytes and NK cells to be inhibited.

外周T细胞淋巴瘤和结外NK/T细胞淋巴瘤研究进展

近年来,得益于新型药物的快速发展,外周T细胞淋巴瘤和结外NK/T细胞淋巴瘤作为中国发病率相对较高的淋巴瘤亚型,相关研究进展备受该领域学者的关注。本文主要对2023年美国临床肿瘤学会(ASCO)、欧洲血液学协会(EHA)年会报道的外周T细胞淋巴瘤和结外NK/T细胞淋巴瘤的研究进展及其对临床实践的重要意义进行综述。

原发性宫颈NK/T细胞淋巴瘤继发周围神经淋巴瘤病1例

目的探讨原发性宫颈NK/T细胞淋巴瘤(NKTCL)继发周围神经淋巴瘤病的影像学特点,以提高对该病的认识,为诊疗决策提供参考。方法回顾性分析1例原发性宫颈NKTCL病人诊治9个月期间的临床资料及CT、MRI、^(18)F-FDG PET/CT影像表现,并复习相关文献。结果病人以阴道流液为首发症状入院。治疗前CT检查发现宫颈明显增大,呈不规则软组织密度肿块影,大小约8.4 cm×6.8 cm×8.3 cm,密度均匀;MRI显示宫颈肿物T_(1)WI呈等信号、FS-T_(2)WI呈高信号、DWI呈高信号、相应ADC图呈低信号,增强扫描病灶呈明显均匀强化,累及阴道上段,双侧髂血管旁见多发淋巴结影;PET/CT显示宫颈肿物及左侧髂血管旁淋巴结代谢异常增高。治疗中PET/CT显示病灶代谢明显降低,范围明显缩小,疗效评价为完全缓解。治疗后继发周围神经淋巴瘤病,PET/CT显示颈椎C2/3水平右侧神经根和大腿左侧坐骨神经走行区域可见新发高代谢病灶,颈椎C2/3神经根处病灶穿刺病理结果为NKTCL浸润。结论原发性宫颈NKTCL的典型影像表现为病灶体积较大、代谢较高,但质地均匀,宫颈黏膜完整。影像学检查在治疗前评价、精准分期、疗效评估、复发监测及指导穿刺部位的选择中发挥着重要作用。

白细胞滤器中的NK及T细胞回收数量及其相关因素研究

目的为了探索白细胞滤器中NK及T细胞回收数量及其相关因素,本项目将分析来源于白细胞滤器及全血的NK细胞及T细胞的数量,并进一步分析白细胞滤器来源的与全血来源的细胞数量是否与年龄、性别等因素相关。方法2023年7月—2024年6月于深圳市血液中心共收集51名重复献血者的全血样本及白细胞滤器(400 mL)。通过PBS缓冲液冲洗白细胞滤器得到白细胞,通过血细胞分析仪分别对来源全血及白细胞滤器来源的细胞进行计数,密度梯度离心法分离得到单个核细胞,流式细胞术分别检测两组来源的CD3-CD56+NK、CD3+CD4+T、CD3+CD8+T细胞的比例,GraphPad Prism 9.0软件进行统计分析。结果细胞计数结果显示,在一定量的冲洗液范围内,白细胞滤器来源的总淋巴细胞数量随着冲洗液体积的增多而增多,但细胞密度随着冲洗液的增多而降低。且白细胞滤器中的总淋巴细胞数量在不同年龄和不同性别的献血者中并未体现出明显的统计学差异(P>0.05)。细胞数量与细胞比例的结果通过计算可知,来源于白细胞滤器的CD3-CD56+NK细胞数量在不同年龄组间存在差异(P<0.05),而在性别分组中未见明显差异(P>0.05)。而来源全血的男性CD3-CD56+NK细胞数量要明显高于女性(P>0.05)。无论是来源白细胞滤器还是全血的CD3+CD4+T细胞、CD3+CD8+T细胞数量在不同年龄组和性别组中都未见明显统计学差异(P>0.05)。结论在一定量的冲洗液范围内,来源白细胞滤器的总淋巴细胞数量与冲洗液的体积相关,CD3+CD4+T细胞、CD3+CD8+T细胞的数量与献血者年龄、性别无明显相关,而CD3-CD56+NK细胞数量与献血者年龄相关。

结外NK/T细胞淋巴瘤伴B淋巴细胞增生2例的临床病理学研究

目的探讨结外NK/T细胞淋巴瘤(extranodal NK/T cell lymphoma,ENKTL)伴B淋巴细胞增生病例的临床病理学特征。方法收集陕西省人民医院2023年6~9月确诊的2例ENKTL伴B淋巴细胞增生病例,采用HE染色、免疫组织化学和原位杂交EB病毒编码的小RNA(Epstein-barr virus encoded small RNA,EBER)检测,观察其组织学特征、免疫表型及原位杂交EBER检测结果,并进行相关文献复习。结果2例老年男性患者,病变部位均为右侧鼻腔,组织学特征为肿瘤细胞弥漫分布,细胞大中小不等,以中大细胞为主,胞核不规则,胞质淡染或透明,核椭圆形,染色质呈颗粒状,核仁不明显,核分裂象较多见,凝固性坏死及凋亡明显;背景见小淋巴细胞灶状聚集,淋巴滤泡散在分布。免疫组织化学CD2,CD3,CD56,TIA-1和颗粒酶B(GrB)阳性表达;CD20,CD79a和PAX-5局灶阳性表达;CD21,CD23及CD35见残存FDC网;CD5阴性表达;Ki-67增殖指数约30%。原位杂交检测EBER肿瘤细胞阳性。病理诊断:ENKTL伴B淋巴细胞增生。结论结外NK/T细胞淋巴瘤伴B淋巴细胞增生少见,尤其是B淋巴细胞增生形成淋巴滤泡时,经验不足很容易造成诊断困扰,需结合临床表现、组织学形态、免疫表型综合分析和诊断。

伴嗜酸性粒细胞增多的结外NK/T细胞淋巴瘤一例

患者,男,53岁。反复双下肢结节红斑1年余,发热1个月余。入院前查血常规嗜酸性粒细胞4.92×109/L(55.3%),首诊为“嗜酸性粒细胞增多症”。入院后查EB病毒DNA阳性,下肢皮损病理检查:以血管为中心较致密的单一核细胞浸润,有异型性改变,及数量较多的嗜酸性粒细胞浸润。免疫组化:CD3(+),Ki67(80%),TIA1(+),Granzyme B(+),Perforin(+),EBER(+)。鼻黏膜及喉肿物活检见异型细胞。诊断:结外NK/T细胞淋巴瘤。

岭南调神助孕针法联合芬吗通对早发性卵巢功能不全患者性激素及NK细胞、T淋巴细胞的影响

目的探讨岭南调神助孕针法联合芬吗通对早发性卵巢功能不全(Premature ovarian insufficiency,POI)患者性激素、自然杀伤细胞(Natural killer cell,NK细胞)和T淋巴细胞的影响。方法选取2022年2月—2023年2月期间就诊于广东医科大学顺德妇女儿童医院门诊的POI患者102例,采用电脑随机数字表法随机分为针刺组和西医组,每组各51例。西医组予以芬吗通口服,针刺组予以岭南调神助孕针法联合芬吗通治疗。3个疗程(3个月经周期)后观察比较两组患者临床疗效及不良反应,评价两组患者治疗前后中医证候积分、改良Kupperman量表评分变化,检测血清性激素指标雌二醇(Estradiol,E2)、卵泡刺激素(Follicle-stimulating hormone,FSH)、黄体生成素(Luteinizing hormone,LH)、抗苗勒管激素(Anti-mullerian hormone,AMH),检测外周血NK细胞与T淋巴细胞亚群(CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+))变化。结果治疗后两组患者中医各项证候评分及总积分均较治疗前明显降低,差异有统计学意义(P<0.05);且针刺组中医各项积分及总积分低于西医组,差异有统计学意义(P<0.05)。治疗后两组患者改良Kupperman评分较治疗前降低,差异有统计学意义(P<0.05);且针刺组改良Kupperman评分明显低于西医组,差异有统计学意义(P<0.05)。治疗后两组患者血清E2、AMH水平较治疗前明显升高,FSH、LH水平较治疗前明显降低,差异有统计学意义(P<0.05);且针刺组E2、AMH水平明显高于西医组,FSH、LH水平明显低于西医组,差异有统计学意义(P<0.05)。治疗后两组患者NK细胞、CD8^(+)较治疗前明显降低,CD4^(+)、CD4^(+)/CD8^(+)较治疗前明显升高,差异有统计学意义(P<0.05);且针刺组NK细胞、CD8^(+)明显低于西医组,CD4^(+)、CD4^(+)/CD8^(+)明显高于西医组,差异有统计学意义(P<0.05)。治疗后针刺组临床总有效率98.04%(50/51)明显高于西医组86.27%(44/51),差异有统计学意义(χ2=8.022,P<0.05)。治疗期间,两组患者不良反应发生率比较,差异有统计学意义(χ2=4.173,P<0.05)。结论岭南调神助孕针法联合芬吗通治疗POI能够改善患者的临床症状及性激素紊乱,调节NK细胞和T淋巴细胞亚群,改善免疫功能,从而保护患者的卵巢功能。