Enhancement of the Human Immunodeficiency Virus (HIV) specific cytotoxic T-cells mechanisms in an HIV-1 and Mycobacterium tuberculosis (Mtb) co-infected individual seems to improve the clinical picture of an individua...Enhancement of the Human Immunodeficiency Virus (HIV) specific cytotoxic T-cells mechanisms in an HIV-1 and Mycobacterium tuberculosis (Mtb) co-infected individual seems to improve the clinical picture of an individual by reducing Acquired Immuno Deficiency Syndrome (AIDS) state progression rate. In this paper, we develop a system of deterministic differential equations representing the immune cells involved in an HIV-1 and Mtb co-infected individual. Results show that although the non-lytic arm of the HIV-1 cytotoxic T-cells affects the co-infection dynamics more than the lytic factors, a combination of both factors results in a more positive reduced progression to the AIDS state. This is due to the increased protection of the CD4<sup>+</sup> T-cells by the CTL mechanisms by further reducing infections and replications by the HIV. Thus, HIV-1 specific CTLs mechanisms’ involvement is here recommended to be part of a solution to the HIV and Mtb co-infection problems.展开更多
Objective: To investigate the correlation of CD4+T cell and tuberculosis test in AIDS patients with different types of tuberculosis. Methods: A total of 127 cases of AIDS complicated with tuberculosis were selected fr...Objective: To investigate the correlation of CD4+T cell and tuberculosis test in AIDS patients with different types of tuberculosis. Methods: A total of 127 cases of AIDS complicated with tuberculosis were selected from our hospital between June 2014 and June 2017 as observation group;50 cases with non-tuberculosis respiratory system who were admitted to our hospital during the same period were selected as control group. The positive rate of TB was examined by T.spotTB test and TST test, and the level of CD4+T lymphocyte was measured by flow cytometry. Results: The positive rate of the observed group After T.spotTB test and TST test was higher than the control group;observation group CD4+T lymphocyte level of 0-100/mm3 was significantly higher than that of the control group;different types of tuberculosis with AIDS showed no significant difference between the T.spotTB test and the TST test in terms of positive rate;There was also no significant difference between the different types of tuberculosis with HIV CD4+T lymphocyte level;the levels of 100-200/mm3 and >200/mm3 CD4+T lymphocytes and T.spotTB test positive and TST positive test were negatively correlated, while the levels of CD4+T lymphocyte 0-100/mm3 and positive T.spotTB test and TST test were positively correlated. Conclusions: the lower level of CD4+T cells in AIDS patients with different types of tuberculosis is positively related to the positive T.spotTB test and TST test.展开更多
Background The health-care workers （HCWs） are at high risk of acquiring infection with Mycobacterium tuberculosis. The objectives of this study were to compare the performance of the T-SPOT.TB and tuberculin skin te...Background The health-care workers （HCWs） are at high risk of acquiring infection with Mycobacterium tuberculosis. The objectives of this study were to compare the performance of the T-SPOT.TB and tuberculin skin test （TST） for latent tuberculosis infection （LTBI）, evaluate diagnostic concordance and risk factors for LTBI, and observe the progression to active tuberculosis （TB） disease among HCWs in a general hospital in Beijing. Methods The prospective cohort study enrolled HCWs in a tertiary general hospital in Beijing, China, to evaluate LTBI with T-SPOT.TB and TST. The subjects were evaluated every 12 months during the 60-month follow-up. Results Of 101 participating HCWs, 96 and 101 had valid TST and T-SPOT.TB results, respectively. Twenty-nine （28.7%, 95% confidence interval （C/）, 19.9%-37.5%） were defined as positive by T-SPOT.TB and 53 （55.2%, 95% CI, 45.2%-64.9%） were defined as positive by TST （using a ≥10 mm cutoff）. An agreement between the two tests was poor （57.3%, K=0.18, 95% CI, 0.01%-0.52%）. In multJvariate analysis, direct exposure to sputum smear-positive TB patients was a significant risk factor for a positive T-SPOT.TB （OR 5.76; 95% CI 1.38-24.00）. Pooled frequency of antigen- specific IFN-y secreting T-cells for subjects who reported direct contact with sputum smear-positive TB patients was significantly higher than that for participants without direct contact （P=0.045）. One of 20 participants with positive result of T-SPOT.TB and TST developed active TB at 24-month follow-up.Conclusion T-SPOT.TB is a more accurate, targeted method of diagnosing LTBI than TST.展开更多
文摘Enhancement of the Human Immunodeficiency Virus (HIV) specific cytotoxic T-cells mechanisms in an HIV-1 and Mycobacterium tuberculosis (Mtb) co-infected individual seems to improve the clinical picture of an individual by reducing Acquired Immuno Deficiency Syndrome (AIDS) state progression rate. In this paper, we develop a system of deterministic differential equations representing the immune cells involved in an HIV-1 and Mtb co-infected individual. Results show that although the non-lytic arm of the HIV-1 cytotoxic T-cells affects the co-infection dynamics more than the lytic factors, a combination of both factors results in a more positive reduced progression to the AIDS state. This is due to the increased protection of the CD4<sup>+</sup> T-cells by the CTL mechanisms by further reducing infections and replications by the HIV. Thus, HIV-1 specific CTLs mechanisms’ involvement is here recommended to be part of a solution to the HIV and Mtb co-infection problems.
文摘Objective: To investigate the correlation of CD4+T cell and tuberculosis test in AIDS patients with different types of tuberculosis. Methods: A total of 127 cases of AIDS complicated with tuberculosis were selected from our hospital between June 2014 and June 2017 as observation group;50 cases with non-tuberculosis respiratory system who were admitted to our hospital during the same period were selected as control group. The positive rate of TB was examined by T.spotTB test and TST test, and the level of CD4+T lymphocyte was measured by flow cytometry. Results: The positive rate of the observed group After T.spotTB test and TST test was higher than the control group;observation group CD4+T lymphocyte level of 0-100/mm3 was significantly higher than that of the control group;different types of tuberculosis with AIDS showed no significant difference between the T.spotTB test and the TST test in terms of positive rate;There was also no significant difference between the different types of tuberculosis with HIV CD4+T lymphocyte level;the levels of 100-200/mm3 and >200/mm3 CD4+T lymphocytes and T.spotTB test positive and TST positive test were negatively correlated, while the levels of CD4+T lymphocyte 0-100/mm3 and positive T.spotTB test and TST test were positively correlated. Conclusions: the lower level of CD4+T cells in AIDS patients with different types of tuberculosis is positively related to the positive T.spotTB test and TST test.
文摘Background The health-care workers （HCWs） are at high risk of acquiring infection with Mycobacterium tuberculosis. The objectives of this study were to compare the performance of the T-SPOT.TB and tuberculin skin test （TST） for latent tuberculosis infection （LTBI）, evaluate diagnostic concordance and risk factors for LTBI, and observe the progression to active tuberculosis （TB） disease among HCWs in a general hospital in Beijing. Methods The prospective cohort study enrolled HCWs in a tertiary general hospital in Beijing, China, to evaluate LTBI with T-SPOT.TB and TST. The subjects were evaluated every 12 months during the 60-month follow-up. Results Of 101 participating HCWs, 96 and 101 had valid TST and T-SPOT.TB results, respectively. Twenty-nine （28.7%, 95% confidence interval （C/）, 19.9%-37.5%） were defined as positive by T-SPOT.TB and 53 （55.2%, 95% CI, 45.2%-64.9%） were defined as positive by TST （using a ≥10 mm cutoff）. An agreement between the two tests was poor （57.3%, K=0.18, 95% CI, 0.01%-0.52%）. In multJvariate analysis, direct exposure to sputum smear-positive TB patients was a significant risk factor for a positive T-SPOT.TB （OR 5.76; 95% CI 1.38-24.00）. Pooled frequency of antigen- specific IFN-y secreting T-cells for subjects who reported direct contact with sputum smear-positive TB patients was significantly higher than that for participants without direct contact （P=0.045）. One of 20 participants with positive result of T-SPOT.TB and TST developed active TB at 24-month follow-up.Conclusion T-SPOT.TB is a more accurate, targeted method of diagnosing LTBI than TST.
