Distribution of natural killer cells and T-lymphocyte subsets in peripheral blood,gallbladder cancer and surrounding tissue

BACKGROUND: The patient with malignant tumor always show immunologic function drawback and ingravescent with tumor development, especially in the aspect of cell-mediated immunity. This study was undertaken to define the relationship between the immune function of local cells and cancer development by investigating the distribution of natural killer (NK) cells and T-lymphocyte subsets in peripheral blood, the cancer tissue and the tissue surrounding gallbladder carcinoma. METHODS: The numbers of CD4(+) and CD8(+) T-lymphocytes and NK cells were measured by flow cytometry in samples taken from gallbladder cancer tissue, the surrounding tissues and peripheral blood of 38 patients, and compared with the numbers in the peripheral blood and gallbladder tissue of 30 patients with cholecystitis as controls. RESULTS: The numbers of CD4(+) and CD8(+) T-cells and NK cells in gallbladder cancer tissues were significantly higher than those in the surrounding tissue and gallbladder with gallstone. However, the ratio of CD4(+)/CD8(+) was lower in the cancer tissue than that in the surrounding tissue and tissue from gallbladders with gallstones. The distribution of CD4(+) and CD8(+) T-cells and NK cells in mucous membrane of cholecystitis gallbladder and that in the tissue surrounding gallbladder cancer were significantly different. CONCLUSIONS: Disproportionate and imbalanced distribution of NK cells and subsets of T-lymphocytes occurs in the mucous membrane proper of gallbladder cancer and surrounding tissue. Although gallbladder cancer tissue has higher expressions of CD4(+), CD8(+) and NK cells, the immune function is low or in an inhibited state. In gallbladder cancer immunization therapy, local cellular immunological function should be enhanced and the protective barrier improved.

Effects of Intraoperative Administration of Dexmedetomidine on the Percentage of T-Lymphocyte Subsets and Natural Killer Cells in Patients with Colorectal Cancer

Study Objective: To observe the effect of dexmedetomidine (DEX) on T-lymphocyte subsets and natural killer (NK) cells in the peripheral blood of perioperative patients with colorectal cancer. Design: A random double-blind control clinical study. Setting: A university hospital. Patients: Forty patients with colorectal cancer, ASA I-П. Interventions: All patients were randomly divided into a DEX group (n = 20) and a control group (n = 20). Before induction of anesthesia, epidural catheters were placed in the L1-L2 or T12-L1 intervertebral spaces. The DEX group received 1 μg/kg of DEX (200 μg/50 ml) intravenously for 15 min prior to the surgery, which was then infused at a rate of 0.5 μg/kg/h until 30 min before the end of the surgery. The control group received an intravenous infusion of saline (50 ml) instead of DEX during the same periods as the DEX group. All patients received routine anesthesia and postoperative analgesia. Measurements: Blood samples from all patients were collected at the following time points: before anesthesia (T0), 24 h after surgery (T1), 48 h after surgery (T2) and 72 h after surgery (T3). Changes in T-lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) and NK cells were determined by flow cytometry. Main Results: Compared with the control group, the percentages of CD3+ and CD4+ cells and the CD4+/CD8+ ratio in the DEX group increased significantly from T1 to T3

Effects of Heat Clearing and Stasis Resolving Method on PTS,Inflammatory Factors and T-lymphocyte Subsets in Peripheral Blood of URM Patients with Suppressed Internal Heat

[Objectives]To explore the effects of heat clearing and stasis resolving method on prethrombotic state,inflammatory factors and T-lymphocyte subsets in peripheral blood of unexplained recurrent miscarriage(URM)patients with suppressed internal heat.[Methods]Thirty cases of URM patients with suppressed internal heat and 30 normal women were collected,and characteristics of changes in peripheral serum D-dimer(D-D),fibrin degradation product(FDP),fibrinogen(FIB),IL-6,IL-10 and TNF-α,CD,CD,CD,CD,CDlevels were detected.URM patients were treated with traditional Chinese medicine for clearing heat and resolving blood stasis for 3 menstrual cycles,and the changes of indicators before and after treatment were observed.[Results]Compared with normal women,the peripheral serum levels of D-D,IL-6,TNF-αand CDin URM patients with suppressed internal heat were increased(P<0.05),while the IL-10 lymphocyte level was significantly decreased(P<0.05);compared with that before treatment,the contents of D-D,IL-6,TNF-αand CDdecreased after 3 menstrual cycles(P<0.05),while the contents of IL-10 and CDT lymphocytes increased significantly(P<0.05).[Conclusions]The heat clearing heat and stasis resolving method can effectively improve the prethrombotic state of URM,and the action mechanism may be related to the regulation of immune and peripheral blood inflammatory factors.

Effects of Radiofrequency Ablation on Lymphocyte Subsets and Type 1/Type 2 T Cell Subpopulations in Patients with Hepatocellular Carcinoma

Objective： To evaluate whether radiofrequency ablation （RFA） might have an influence on immune status in hepatocellular carcinoma （HCC） patients. Methods： We measured the T lymphocytes, B lymphocyte and NK cells, and determined the population of Thl, Th2, Tcl and Tc2 of peripheral blood samples taken from 26 HCC patients before and after RFA. Results： The proportion of Typel cells （Thl and Tcl） and NK cells were significantly increased after RFA, especially in patients of the following subgroups： male, age〉55 years, pathological grade Ⅰ-Ⅱ tumor, clinical stage Ⅰ-Ⅱ or Child-Pugh A and B. Conclusion： TypeⅠ cells and NK cells in HCC patients were increased in a short period after RFA.

Changes in T lymphocyte subsets after severe traumatic brain injury

BACKGROUND： Besides local changes of cranial parenchymal cells, hemorrhage, etc., severe traumatic brain injuries also cause the changes of total body fluid and various functions, and the changes of lymphocytes and T lymphocyte subsets should be paid more attention to. OBJECTIVE： To reveal the changing laws of T lymphocyte subsets after severe traumatic brain injury, and compare with mild to moderate brain injury. DESIGN： A comparative observation. SETTINGS： Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City; Central Laboratory of Shenzhen Hospital of Prevention and Cure for Chronic Disease. PARTICIPANTS： All the subjects were selected from the Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City from August 2002 to August 2005. Thirty patients with severe brain injury, whose Glasgow coma score （GCS） was ≤ 8 points, were taken as the experimental group, including 21 males and 9 females, aging 16 - 62 years. Meanwhile, 30 patients with mild traumatic brain injury were taken as the control group （GCS ranged 14- 15 points）, including 18 males and 12 females, aging 15 -58 years. All the subjects were in admission at 6 hours after injury, without disease of major organs before injury Informed consents were obtained from all the patients or their relatives. METHODS： （1） The T lymphocytes and the subsets in peripheral blood were detected with immunofluorescent tricolor flow cytometry at l, 3, 7 and 14 days after injury in both groups. （2） The conditions of pulmonary infections were observed at 4 days after injury. The differences of measurement data were compared with the t test. MAIN OUTCOME MEASURES： Changes of T lymphocytes subsets at 1 - 14 days after severe and mild or moderate traumatic injury. RESULTS： Finally, 28 and 25 patients with mild to moderate traumatic brain injury, whereas 25 and 21 patients with severe traumatic brain injury were analyzed at 7 and 14 days respectively, and the missed ones died due to the development of disease. （1） Changes of T lymphocyte subsets： At 1 and 3 days after injury, CD3, CD4, CD8, CD4/CD8 began to decrease, whereas CD8 increased in the experimental group, which were very significantly different from those in the control group （t =2.77 - 3.26, P 〈 0.01）, and began to recover at 7 days, which were significantly different from those in the control group （t = 2.06 - 2.24, P 〈 0.05）, and generally recovered to the normal levels at 14 days （P 〉 0.05）. （2） Conditions of pulmonary infections： At 4 days after injury, the rate of pulmonary infection was significantly different between the experimental group and control group [73% （22/30）, 0, x2=37.29, P 〈 0.01]. CONCLUSION： Patients with severe traumatic brain injury suffer from damages of cellular immune function at early period （within 7 days）, and they are easily to be accompanied by pulmonary infections.

STUDY OF T－LYMPHOCYTE SUBSETS AND INTERLEUKIN－2 AND INTERLEUKIN－2 RECEPTOR OF RESPIRATORY SYNCYTIAL VIRUS PNEUMONIA

T-Lymphocyte subsets and humoral immune and the activity of IL-2 and IL-2R in respiratory syncytial virus (RSV) pneumonia in 26 cases were tested. The result showed in the patients with RSV pneumonia the averages of T3 and T4 were 37.56±1.46% and 27. 15±8. 02% respectively,They were significantly lower than 53.4 ±9.2% and 35.5±7.7% of averages of T3 and T4 in healthy controlled group (both. P< 0. 001 ), the average of T3 was 22. 73±7.06%, it was lower an 26. 7±6. 3 % of T8 in controlled group (P<0. 02 );the ratio of T4/T8 was 1. 245±0. 399 ,there was no significant difference from 1. 35 ±0. 17 of the ratio in controlled group (P > 0. 1). The mean value of IgG was 1. 177± 0. 3685g/L, it was significantly lower than 1. 427± 0. 498g/L of that in controlled group(P < 0. 005). The mean values of IgA and IgM were 0. 1136±0.0393g/L and 0. 768±0. 353g/L respectively, they were significantly lower than 0. 2706 ±0. 876g/L and 0. 122±0. 061g/L of IgA and IgM in controlled group. The activity of IL-2 and IL-2R were 17. 46 ±5. 79%, and 28. 32 ±5. 924% respectively, they were significantly lower than 30. 22 ±14. 55% and 39. 53±8. 61 % of those in healthy group (both P < 0. 001). The severe the pneuumonia, the greater the lowering of IL-2 and IL-2R. These about results suggested that RSV could greatly suppress the immune function of the patients, inducing secondary immunodeficiency, leading to repeated breather and asthma.

Clinical characteristics and T-lymphocyte subsets in 48 acquired immune deficiency syndrome patients with cytomegalovirus infections

Objective To investigate the clinical features and Tlymphocytes subsets in patients with acquired immunedeficiency syndrome ( AIDS ) and cytomegalovirus(CMV) infection. Methods A total of 48 hospitalizedpatients with human immunodeficiency virus (HIV)-1 /AIDS and CMV infections were recruited at Peking UnionMedical College Hospital from Jan 2010 to Aug 2017.Their clinical features and immune function were retrospectivelyanalyzed. Patients with only HIV/AIDS inprevious study were recruited as controls. Results All 48patients were at C3 stage, including 36 men and12 women. Five of them were younger than 30 years old,33 cases within 31 - 50 years old,and 10 cases olderthan 50 years old.

Endothelial function and T-lymphocyte subsets in patients with overlap syndrome of chronic obstructive pulmonary disease and obstructive sleep apnea

Background:The coexistence of chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea (OSA) is termed overlap syndrome (OS).COPD and OSA both have increased risks of developing cardiovascular diseases.This study aimed to explore if patients with OS exhibited a higher prevalence of cardiovascular complications,and if patients with OS exhibited vascular endothelial dysfunction and abnormalities in the cellular immune function of T lymphocytes.Methods:Totally 25 patients with stable COPD (COPD group),25 patients with OSA (OSA group),25 patients with OS (OS group),and 20 healthy adults (control group) were enrolled between January 2017 and December 2017 from the Respiratory Department of Tianjin Medical University General Hospital.The clinical characteristics of the four groups were collected and the expression levels of soluble vascular cell adhesion molecule-1 (sVCAM-1),tumor necrosis factor-α(TNF-α),and T-lymphocyte subsets were detected.One-way analysis of variance,x^2 test and Pearson correlation were used to manage the data.Results:The prevalence of hypertension and coronary heart disease was significantly higher in the OS group than in the control,OSA,and COPD groups (x^2 =20.69,P < 0.05 and x^2 =11.03,P < 0.05,respectively).The levels of sVCAM-1 and TNF-α were significantly higher in the OS group than in other groups (F =127.40,P < 0.05 and F =846.77,P < 0.05,respectively).The percentage of CD4+ lymphocytes and CD4+/CD8+ were both significantly lower in the OS group than in any other group (F =25.40,P < 0.05 and F =75.08,P < 0.05,respectively).There were significantly negative correlations in the levels of sVCAM-1 and TNF-α with CD4^+/CD8^+ lymphocytes (r =-0.77,P < 0.05 and r =-0.83,P < 0.05,respectively).Conclusions:The prevalence of hypertension and coronary heart disease was higher in patients with OS than in patients with either OSA or COPD alone.Patients with OS exhibited more severe vascular endothelial injury,stronger inflammatory response,and lower cellular immune function.

Regulatory Effect of Langchuang Serial Recipes on T-Lymphocyte Subsets Th and Tc in Patients with Systemic Lupus Erythematosus

Objective： To study the principle of clearing Fei （肺）, cooling blood, and detoxification as well as nourishing yin and moisening Fei （abbr. as CCD-NM） in regulating the levels of peripheral T-lymphocyte subsets Th and Tc cells to explore its mechanism for lowering the incidence of infection in patients with systemic lupus erythematosus iSLE）. Methods： Sixty SLE patients without complicated infection were assigned to the treatment group and the control group, 30 in each group. The control group was treated with Western medicine alone, while the treatment group was treated with the same program of Western medicine, but additionally administered with either Langchuang No.1 （狼疮 Ⅰ号） or 2 （狼疮 Ⅱ 号）, serial concerted Chinese recipes, applied respectively in patients in the active stage or in the resting stage. The total time of treatment for both groups was 1 year. Further, a healthy control group was set up with 20 healthy subjects. The expressions of Thl, Th2, and Tcl and Tc2 cells in peripheral blood were detected and compared with those in the healthy control group. Results： （1） As compared with the healthy control group, ratios of Thl/Th2 and Tcl/Tc2 in SLE patients, whether complicated with infection or not, were significantly lower （P〈0.05 or P〈0.01）. （2） Comparison between patients with complications and those uncomplicated with infection showed that the two ratios and Thl expression were lower and Tc2 was higher in the former than those in the latter （all P〈0.05）. （3） Ratios of Thl/Th2 and Tcl/Tc2 increased after treatment in patients of both the treatment group and the control group （P〈0.05 and P〈0.01）, but the changes in the treatment group were more significant （P〈0.05）. Conclusion： The principle of CCD-NM could regulate the Th and Tc subsets toward equilibrium in SLE patients, which might be one of the mechanisms of action for alleviating complicated infection.

IFNα-1b联合阿昔洛韦治疗对IM患儿EBV-DNA拷贝数转阴率及T淋巴细胞亚群的影响

目的研究干扰素α-1b(Interferonα-1b,IFNα-1b)联合阿昔洛韦治疗对传染性单核细胞增多症(Infectious mononucleosis,IM)患儿EBV-DNA拷贝数转阴率及T淋巴细胞亚群的影响。方法收集2018年5月至2021年5月在本院进行治疗的103例IM患儿的临床资料进行本次研究。按照不同治疗方式列为研究组(n=52,IFNα-1b联合阿昔洛韦治疗)和对照组(n=51,阿昔洛韦治疗)。比较两组EBV-DNA拷贝数转阴率、T淋巴细胞亚群水平及不良反应情况。结果(1)研究组患者EBV-DNA拷贝数转阴率(96.15%)显著高于对照组患者EBV-DNA拷贝数转阴率(78.43%),差异具有统计学意义(P<0.05)。(2)治疗后,两组CD^(3+)、CD^(8+)较治疗前降低,CD^(4+)、CD^(4+)/CD^(8+)较治疗前升高,且以研究组变化最为明显,差异具有统计学意义(P<0.05)。(3)研究组不良反应率为11.54%,对照组不良反应率为7.84%,两组对比差异不具有统计学意义(P>0.05)。结论?IFNα-1b联合阿昔洛韦治疗IM患儿的效率高,能够有效改善患者的T淋巴细胞亚群水平,且具有一定安全性,值得推广使用。

外周血CD8^(+)T淋巴细胞亚群检测对过敏性鼻炎临床疗效的评估价值

目的探讨外周血CD8^(+)T淋巴细胞亚群检测对过敏性鼻炎(AR)临床疗效的评估价值。方法前瞻性选取2021年3月~2022年2月温州市中西医结合医院耳鼻咽喉科门诊治疗的120例AR患者和40例健康志愿者,分别设为研究组和对照组,比较两组外周血CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率;研究组接受鼻用激素联合口服抗组胺药物治疗方案,根据治疗效果分为有效组(n=107)和无效组(n=13),比较有效组和无效组治疗前CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率;Pearson相关性分析法分析外周血CD8^(+)T淋巴细胞亚群水平与鼻部症状总评分(totalnose symptomscore,TNSS)的相关性;以受试者工作特征(ROC)曲线分析治疗前CD8^(+)T淋巴细胞亚群对AR临床疗效的预测价值。结果研究组治疗前外周血CD8^(+)CD28^(+)T细胞比率高于对照组,CD8^(+)CD28-T细胞比率低于对照组(P<0.05)。研究组总有效率为89.17%;有效组治疗前CD8^(+)CD28^(+)T细胞比率低于无效组,CD8^(+)CD28-T细胞比率高于无效组(P<0.05);Pearson相关性分析显示,CD8^(+)CD28^(+)T细胞比率与TNSS评分呈正相关(r=0.324,P=0.006),CD8^(+)CD28-T细胞比率与TNSS评分呈负相关(r=-0.541,P=0.000)。ROC曲线结果显示,CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞、CD8^(+)CD28-T细胞比率评估AR治疗无效的曲线下面积(AUC)(95%CI)分别为0.647(0.555~0.732)、0.683(0.592~0.765)、0.911(0.845~0.955),其中CD8^(+)CD28-T细胞的评估效能优于CD8^(+)T细胞、CD8^(+)CD28^(+)T细胞比率(P<0.05)。结论AR患者外周血CD8^(+)CD28^(+)T细胞比率升高,CD8^(+)CD28-T细胞比率降低,CD8^(+)T细胞比率相对稳定,治疗前CD8^(+)CD28-T细胞比率可作为预测AR患者的临床疗效的参考指标。

腺样体肥大患儿机体免疫功能变化研究

<正>腺样体也称咽扁桃体或增殖体,属于淋巴组织,表面呈桔瓣样。正常生理情况下,儿童6~7岁时腺样体发育至最大,青春期后逐渐萎缩,成人后基本消失~[1]。腺样体位于鼻咽顶后壁处,病理性肥大易引起鼻堵、张口呼吸、耳闷、听力下降等症状,最终造成慢性鼻-鼻窦炎、阻塞性睡眠呼吸暂停低通气综合征及分泌性中耳炎等疾病,给儿童的颌面骨发育、智力发育造成严重影响。

稀释式自体输血与少白细胞红细胞输注对肿瘤患者围术期T淋巴细胞亚群、NK细胞影响的比较

目的 探讨输入异体全血、异体少白细胞红细胞和等容血液稀释自体输血对围术期 T淋巴细胞亚群、NK细胞的影响。方法 选择胃癌、结肠癌或直肠癌根治手术患者 60例 ,随机均分为 3组。1H1组 :术中输异体全血 40 0～ 60 0 m l;2 H2 组 :术中输异体少白细胞红细胞 2～ 3单元 ;3 A组 :于麻醉后手术切皮前经桡动脉放血 40 0～ 60 0 ml,同时经静脉输入相当容量的羟乙基淀粉 ,手术后半阶段将自体血回输。分别于术前、手术后即刻、术后 1天、术后 5天抽取静脉血 ,用流式细胞仪测定 T细胞亚群和 NK细胞的数量。结果 三组术后第 1天 CD3、CD4、 CD4/ CD8、 NK细胞较术前显著减少 (P <0 .0 5或 P <0 .0 1) ,其中 H1组和 H2 组较 A组减少更明显 (P <0 .0 5)。术后第 5天异体输血组 CD3、 CD4、 CD4/ CD8、 NK细胞仍较术前显著减少 ,而 H2 组和 A组均基本恢复正常 ,且 A组 CD3、 CD4、高于 H2 组 (P <0 .0 5)。结论 围术期异体输血严重抑制患者免疫功能 ,少白细胞红细胞和等容血液稀释自体输血对患者的免疫功能抑制轻 ,且术后恢复较快 。

肠道易激综合征患者临床免疫功能研究

本文对30例肠道易激综合征患者外周血T细胞亚群、NK活性、IL-2R表达及血清免疫球蛋白和补体C_3含量进行检测,并与30例正常对照比较。结果表明患者外周血CD_8细胞减少、CD_4/CD_8比值升高、NK活性降低、IL-2R表达阳性率降低及血清IgG含量升高,提示肠道易激综合征患者机体免疫功能较正常降低。

急性肠炎患儿自身免疫功能的监测与分析

【目的】分析和监测急性肠炎患儿细胞及体液免疫功能的变化。【方法】选取2008年1月至2013年12月本院收治的急性肠炎患儿50例作为观察组,选取同期50例经本院门诊体检显示为健康儿童作为对照组,测定两组儿童的 T 淋巴细胞亚群、血清免疫球蛋白及红细胞免疫功能,并进行比较。【结果】T 淋巴细胞亚群的检测结果比较显示,观察组患儿病发期间 CD3＋、CD4＋百分率较对照组明显降低（P ＜0.05）；血清IgG、IgA、IgM 比较显示,观察组患儿病发期间血清 IgG、IgA、IgM 含量均明显低于对照组（P ＜0.05）；红细胞免疫功能测定结果比较显示,观察组患儿病发期间 RBC-CR1R 明显低于对照组（P ＜0.05）,RBC-ICR 两组之间比较无显著性差异（P ＞0.05）。【结论】急性肠炎患儿细胞免疫和体液免疫功能发生了明显紊乱,其可能参与了急性肠炎的感染过程；治疗时应维持患儿的免疫功能稳定,以期提高患儿抗感染能力。

传染性单核细胞增多症患儿T细胞亚群变化及临床意义

目的:通过检测传染性单核细胞增多症(IM)患儿外周血T细胞亚群,探讨其免疫功能与疾病的关系。早期了解疾病的发生并加以干预以减少不良的预后。方法:采用流式细胞仪对观察组18例IM患儿进行T细胞亚群测定,同时随机抽取20例健康儿童作为对照组。另外对观察组18例患儿中的10例进行治疗2周后T细胞亚群的测定。结果:观察组与对照组CD4,CD4/CD8均明显下降(P<0.001),CD8明显升高(P<0.001)。观察组的10例治疗2周后T细胞亚群的测定值与入院时比较有显著差异(P<0.001)。结论:EB病毒感染引起机体免疫功能改变是IM发病的关键,早期诊断治疗、长期随访都有积极的意义。

鼻咽癌患者外周血NK细胞及T细胞亚群的变化

目的了解和分析鼻咽癌患者机体免疫功能。方法采用LDH释放法和免疫花环法对 6 9例鼻咽癌患者外周血NK细胞和T细胞亚群进行观察。结果与正常人相比 ,鼻咽癌患者NK细胞活性、T3 、T4 、T4 /T8细胞比值明显低于正常人 (P <0 .0 1) ,T8与正常人相比稍有下降 (P >0 .0 5 )。结论鼻咽癌患者免疫系统的平衡制约失调 。

弯套针旋转多向注射法注射臭氧治疗腰椎间盘突出症临床观察

目的探讨椎间盘内注射臭氧治疗腰椎间盘突出症(LDH)的机理及临床疗效。方法 120例LDH患者随机分成2组,除外5例脱落病例,观察组57例,对照组58例。观察组采用弯套针旋转多向注射法对突出椎间盘内注射臭氧治疗;对照组采用改良侧隐窝注药治疗。治疗后3个月评定疗效;同时于治疗前及治疗后3个月时检测LDH患者血清免疫球蛋白和T淋巴细胞亚群水平,并对15例健康体检者进行上述指标检测作为正常组进行对比。结果观察组和对照组的痊愈显效率分别为84.2%和63.8%,2组间比较具有显著性差异(x^2=6.22,P<0.05)。治疗前2组Ig G和Ig M水平均明显高于正常组(P<0.01),Ig A水平与正常组比较无明显差异(P>0.05),CD_3^+、CD_4^+、CD_8^+、CD_4^+/CD_8^+水平均明显低于正常组(P<0.01)。治疗后2组Ig G和Ig M水平呈下降趋势,但观察组下降更明显;T淋巴细胞亚群各项指标均呈上升趋势,但观察组上升更明显;且观察组各项指标均接近正常水平,与对照组比较有显著性差异(P<0.01)。结论弯套针旋转多向注射法注射臭氧治疗LDH具有比侧隐藏窝注射曲安奈德更好的调节患者的免疫功能的作用,因而能更好地改善神经根免疫性炎症反应,缓解腰腿痛症状,取得较好的疗效。

穴位注射黄芪注射液对XELOX方案化疗胃癌患者免疫功能的影响

目的:探讨穴位注射黄芪注射液对XELOX方案对胃癌患者免疫功能的影响。方法:将67例胃癌患者分为2组,观察组采用穴位注射黄芪注射液联合XELOX化疗方案治疗;对照组仅采用XELOX化疗方案治疗;对比2组患者治疗前后免疫指标变化及药物毒副作用发生情况。结果:观察组治疗后CD_3^+、CD_4^+、CD_8^+、CD_4^+/CD_8^+、CD_(16)^+/CD_(56)^+水平均得到显著改善(P<0.05);效果显著优于对照组,差异有统计学意义(P<0.05));对照组治疗后上述指标无显著性改善,差异无显著性意义(P>0.05);观察组治疗后IgA、IgM、IgG水平与治疗前比较差异无显著性意义(P>0.05);对照组治疗后IgM、IgA水平显著低于治疗前且显著低于观察组治疗后,经统计学处理,差异均具有显著性意义(P<0.05)。观察组白细胞抑制、血红蛋白抑制情况显著低于对照组,经统计学处理,差异有显著性意义(P<0.05)。结论:穴位注射黄芪注射液能显著改善XELOX方案化疗胃癌患者的免疫功能減轻药物毒副作用。

幼年特发性关节炎和系统性红斑狼疮患儿的免疫特征

目的:分析幼年特发性关节炎(JIA)和系统性红斑狼疮(SLE)患儿细胞及体液免疫特征。方法:选择JIA患儿66例(JIA组)、SLE患儿50例(SLE组)及健康儿童50例(对照组),检测JIA组及SLE组儿童血常规(Hb、WBC、PLT)及CRP水平,比较3组儿童淋巴细胞亚群和免疫球蛋白及补体水平。结果:JIA组CRP及PLT升高率明显高于SLE组(P<0.05),JIA组CD4^+比例高于对照组(P<0.05),NK比例低于对照组(P<0.05),SLE组CD8^+高于对照组(P<0.05),NK比例低于对照组(P<0.05);JIA组IgG、IgM、lg A及C_3均高于对照组(P<0.05),SLE组IgG、IgM、lg A均高于对照组(P<0.05),C_3、C_4均低于对照组(P<0.05),SLE组C_3、C_4均低于JIA组(P<0.05);JIA组CD4^+与JADAS-27正相关(P<0.05),SLE组CD8^+与SLEDAI正相关(P<0.05),CD8^+在判断SLE疾病活动中的ROC曲线下面积达0.858。结论:SLE和JIAT均存在T细胞亚群失衡和B细胞异常活化以及NK细胞功能降低,但两种疾病在主导失衡的T细胞亚群、NK功能不足以及补体紊乱方面有明显的不同。