Distribution of natural killer cells and T-lymphocyte subsets in peripheral blood,gallbladder cancer and surrounding tissue

BACKGROUND: The patient with malignant tumor always show immunologic function drawback and ingravescent with tumor development, especially in the aspect of cell-mediated immunity. This study was undertaken to define the relationship between the immune function of local cells and cancer development by investigating the distribution of natural killer (NK) cells and T-lymphocyte subsets in peripheral blood, the cancer tissue and the tissue surrounding gallbladder carcinoma. METHODS: The numbers of CD4(+) and CD8(+) T-lymphocytes and NK cells were measured by flow cytometry in samples taken from gallbladder cancer tissue, the surrounding tissues and peripheral blood of 38 patients, and compared with the numbers in the peripheral blood and gallbladder tissue of 30 patients with cholecystitis as controls. RESULTS: The numbers of CD4(+) and CD8(+) T-cells and NK cells in gallbladder cancer tissues were significantly higher than those in the surrounding tissue and gallbladder with gallstone. However, the ratio of CD4(+)/CD8(+) was lower in the cancer tissue than that in the surrounding tissue and tissue from gallbladders with gallstones. The distribution of CD4(+) and CD8(+) T-cells and NK cells in mucous membrane of cholecystitis gallbladder and that in the tissue surrounding gallbladder cancer were significantly different. CONCLUSIONS: Disproportionate and imbalanced distribution of NK cells and subsets of T-lymphocytes occurs in the mucous membrane proper of gallbladder cancer and surrounding tissue. Although gallbladder cancer tissue has higher expressions of CD4(+), CD8(+) and NK cells, the immune function is low or in an inhibited state. In gallbladder cancer immunization therapy, local cellular immunological function should be enhanced and the protective barrier improved.

Effects of Intraoperative Administration of Dexmedetomidine on the Percentage of T-Lymphocyte Subsets and Natural Killer Cells in Patients with Colorectal Cancer

Study Objective: To observe the effect of dexmedetomidine (DEX) on T-lymphocyte subsets and natural killer (NK) cells in the peripheral blood of perioperative patients with colorectal cancer. Design: A random double-blind control clinical study. Setting: A university hospital. Patients: Forty patients with colorectal cancer, ASA I-П. Interventions: All patients were randomly divided into a DEX group (n = 20) and a control group (n = 20). Before induction of anesthesia, epidural catheters were placed in the L1-L2 or T12-L1 intervertebral spaces. The DEX group received 1 μg/kg of DEX (200 μg/50 ml) intravenously for 15 min prior to the surgery, which was then infused at a rate of 0.5 μg/kg/h until 30 min before the end of the surgery. The control group received an intravenous infusion of saline (50 ml) instead of DEX during the same periods as the DEX group. All patients received routine anesthesia and postoperative analgesia. Measurements: Blood samples from all patients were collected at the following time points: before anesthesia (T0), 24 h after surgery (T1), 48 h after surgery (T2) and 72 h after surgery (T3). Changes in T-lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) and NK cells were determined by flow cytometry. Main Results: Compared with the control group, the percentages of CD3+ and CD4+ cells and the CD4+/CD8+ ratio in the DEX group increased significantly from T1 to T3

The peri-operative changes of T-lymphocyte subsets in the elder esophageal carcinoma patients

Objective:The aim of the study was to observe and compare the trend of T-lymphocyte subsets in the elder and adult esophageal carcinoma patients postoperatively. Methods: Forty-four esophageal carcinoma patients, classified as ASA physical status I-II, were divided into two groups. Twenty-two patients aged than 65 years were grouped as elder patient group, and the rest twenty-two patients, served as adult patient group, were younger than 60 years old. The jugular venous blood samples were collected before induction of anesthesia (T1) as baseline, after completion of surgery (T2), on the first, second and fifth postoperative days (T3, T4 and T5), respectively. The blood counts of CD3+, CD4+ and CD8+T-lymphocyte were measured by flow cytometer. Results: Compared with the baseline level, the blood CD3+, CD4+ and CD4/CD8 levels were significantly decreased immediately after surgery, on the first and second postoperative days in the two groups (P<0.01), which returned to baseline values on the fifth postoperative day (P>0.05). On the fifth postoperative day, CD3+, CD4+ and CD4/CD8 levels in adult group were significantly higher than those in elder group (P<0.05). Conclusion: The postoperative immune function in elder patients recovered more slowly than that in adult.

Effects of Heat Clearing and Stasis Resolving Method on PTS,Inflammatory Factors and T-lymphocyte Subsets in Peripheral Blood of URM Patients with Suppressed Internal Heat

[Objectives]To explore the effects of heat clearing and stasis resolving method on prethrombotic state,inflammatory factors and T-lymphocyte subsets in peripheral blood of unexplained recurrent miscarriage(URM)patients with suppressed internal heat.[Methods]Thirty cases of URM patients with suppressed internal heat and 30 normal women were collected,and characteristics of changes in peripheral serum D-dimer(D-D),fibrin degradation product(FDP),fibrinogen(FIB),IL-6,IL-10 and TNF-α,CD,CD,CD,CD,CDlevels were detected.URM patients were treated with traditional Chinese medicine for clearing heat and resolving blood stasis for 3 menstrual cycles,and the changes of indicators before and after treatment were observed.[Results]Compared with normal women,the peripheral serum levels of D-D,IL-6,TNF-αand CDin URM patients with suppressed internal heat were increased(P<0.05),while the IL-10 lymphocyte level was significantly decreased(P<0.05);compared with that before treatment,the contents of D-D,IL-6,TNF-αand CDdecreased after 3 menstrual cycles(P<0.05),while the contents of IL-10 and CDT lymphocytes increased significantly(P<0.05).[Conclusions]The heat clearing heat and stasis resolving method can effectively improve the prethrombotic state of URM,and the action mechanism may be related to the regulation of immune and peripheral blood inflammatory factors.

Effects of Radiofrequency Ablation on Lymphocyte Subsets and Type 1/Type 2 T Cell Subpopulations in Patients with Hepatocellular Carcinoma

Objective： To evaluate whether radiofrequency ablation （RFA） might have an influence on immune status in hepatocellular carcinoma （HCC） patients. Methods： We measured the T lymphocytes, B lymphocyte and NK cells, and determined the population of Thl, Th2, Tcl and Tc2 of peripheral blood samples taken from 26 HCC patients before and after RFA. Results： The proportion of Typel cells （Thl and Tcl） and NK cells were significantly increased after RFA, especially in patients of the following subgroups： male, age〉55 years, pathological grade Ⅰ-Ⅱ tumor, clinical stage Ⅰ-Ⅱ or Child-Pugh A and B. Conclusion： TypeⅠ cells and NK cells in HCC patients were increased in a short period after RFA.

mIL-2R,T cell subsets & hepatitis C

AIM: To study the levels of membrane interleukin-2 receptor (mIL-2R) and T cell subsets in peripheral blood mononuclear cells (PBMC) from patients with hepatitis C and their role in the pathogenesis of hepatitis C. METHODS: The levels of mIL-2R and T cells subsets in PBMC were detected by biotin- streptatividin (BSA) technique before and after stimulation with PHA in 203 patients with hepatitis C with HCV-RNA(+), anti-HCV(+), anti-HCV(-). RESULTS: The total expressive levels of mIL-2R before and after stimulation with PHA(0.03+/-0.01, 0.03+/-0.02, 0.04+/-0.02, 0.36+/-0.03), and T cell subsets in PBMC (0.62+/-0.06, 0.37+/-0.05, 0.35+/-0.07) were all lower in patients with hepatitis C than those in normal controls (0.66+/-0.07, 0.41+/-0.06, 0.31+/-0.05, P【0.01). Among the patients, the levels of mIL-2R were lower in silence than those in situation of PHA inducting (P【0.01). However, the levels of mIL-2R were similar in acute hepatitis C to that in chronic hepatitis C (P】0.05). The levels of CD(3)(+), CD(4)(+), CD(4)(+)/CD(8)(+) were lower and CD(8)(+) was higher in patients with acute and chronic hepatitis C with anti-HCV(+) than those in normal controls (0.62+/-0.06, 0.37+/-0.05, 0.35+/-0.07, 1.18+/-0.30, 0.61+/-0.07, 0.37+/-0.05, 1.39+/-0.33, 0.31+/-0.05, P【0.05-P【0.01). CONCLUSION: The cellular immunity is obviously changed in patients with hepatitis C. The levels of mIL-2R and activation of T cells are closely associated with chronicity of hepatitis C.

Changes in T lymphocyte subsets after severe traumatic brain injury

BACKGROUND： Besides local changes of cranial parenchymal cells, hemorrhage, etc., severe traumatic brain injuries also cause the changes of total body fluid and various functions, and the changes of lymphocytes and T lymphocyte subsets should be paid more attention to. OBJECTIVE： To reveal the changing laws of T lymphocyte subsets after severe traumatic brain injury, and compare with mild to moderate brain injury. DESIGN： A comparative observation. SETTINGS： Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City; Central Laboratory of Shenzhen Hospital of Prevention and Cure for Chronic Disease. PARTICIPANTS： All the subjects were selected from the Department of Neurosurgery, Longgang District Buji People＇s Hospital of Shenzhen City from August 2002 to August 2005. Thirty patients with severe brain injury, whose Glasgow coma score （GCS） was ≤ 8 points, were taken as the experimental group, including 21 males and 9 females, aging 16 - 62 years. Meanwhile, 30 patients with mild traumatic brain injury were taken as the control group （GCS ranged 14- 15 points）, including 18 males and 12 females, aging 15 -58 years. All the subjects were in admission at 6 hours after injury, without disease of major organs before injury Informed consents were obtained from all the patients or their relatives. METHODS： （1） The T lymphocytes and the subsets in peripheral blood were detected with immunofluorescent tricolor flow cytometry at l, 3, 7 and 14 days after injury in both groups. （2） The conditions of pulmonary infections were observed at 4 days after injury. The differences of measurement data were compared with the t test. MAIN OUTCOME MEASURES： Changes of T lymphocytes subsets at 1 - 14 days after severe and mild or moderate traumatic injury. RESULTS： Finally, 28 and 25 patients with mild to moderate traumatic brain injury, whereas 25 and 21 patients with severe traumatic brain injury were analyzed at 7 and 14 days respectively, and the missed ones died due to the development of disease. （1） Changes of T lymphocyte subsets： At 1 and 3 days after injury, CD3, CD4, CD8, CD4/CD8 began to decrease, whereas CD8 increased in the experimental group, which were very significantly different from those in the control group （t =2.77 - 3.26, P 〈 0.01）, and began to recover at 7 days, which were significantly different from those in the control group （t = 2.06 - 2.24, P 〈 0.05）, and generally recovered to the normal levels at 14 days （P 〉 0.05）. （2） Conditions of pulmonary infections： At 4 days after injury, the rate of pulmonary infection was significantly different between the experimental group and control group [73% （22/30）, 0, x2=37.29, P 〈 0.01]. CONCLUSION： Patients with severe traumatic brain injury suffer from damages of cellular immune function at early period （within 7 days）, and they are easily to be accompanied by pulmonary infections.

STUDY OF T－LYMPHOCYTE SUBSETS AND INTERLEUKIN－2 AND INTERLEUKIN－2 RECEPTOR OF RESPIRATORY SYNCYTIAL VIRUS PNEUMONIA

T-Lymphocyte subsets and humoral immune and the activity of IL-2 and IL-2R in respiratory syncytial virus (RSV) pneumonia in 26 cases were tested. The result showed in the patients with RSV pneumonia the averages of T3 and T4 were 37.56±1.46% and 27. 15±8. 02% respectively,They were significantly lower than 53.4 ±9.2% and 35.5±7.7% of averages of T3 and T4 in healthy controlled group (both. P< 0. 001 ), the average of T3 was 22. 73±7.06%, it was lower an 26. 7±6. 3 % of T8 in controlled group (P<0. 02 );the ratio of T4/T8 was 1. 245±0. 399 ,there was no significant difference from 1. 35 ±0. 17 of the ratio in controlled group (P > 0. 1). The mean value of IgG was 1. 177± 0. 3685g/L, it was significantly lower than 1. 427± 0. 498g/L of that in controlled group(P < 0. 005). The mean values of IgA and IgM were 0. 1136±0.0393g/L and 0. 768±0. 353g/L respectively, they were significantly lower than 0. 2706 ±0. 876g/L and 0. 122±0. 061g/L of IgA and IgM in controlled group. The activity of IL-2 and IL-2R were 17. 46 ±5. 79%, and 28. 32 ±5. 924% respectively, they were significantly lower than 30. 22 ±14. 55% and 39. 53±8. 61 % of those in healthy group (both P < 0. 001). The severe the pneuumonia, the greater the lowering of IL-2 and IL-2R. These about results suggested that RSV could greatly suppress the immune function of the patients, inducing secondary immunodeficiency, leading to repeated breather and asthma.

Endothelial function and T-lymphocyte subsets in patients with overlap syndrome of chronic obstructive pulmonary disease and obstructive sleep apnea

Background:The coexistence of chronic obstructive pulmonary disease (COPD) and obstructive sleep apnea (OSA) is termed overlap syndrome (OS).COPD and OSA both have increased risks of developing cardiovascular diseases.This study aimed to explore if patients with OS exhibited a higher prevalence of cardiovascular complications,and if patients with OS exhibited vascular endothelial dysfunction and abnormalities in the cellular immune function of T lymphocytes.Methods:Totally 25 patients with stable COPD (COPD group),25 patients with OSA (OSA group),25 patients with OS (OS group),and 20 healthy adults (control group) were enrolled between January 2017 and December 2017 from the Respiratory Department of Tianjin Medical University General Hospital.The clinical characteristics of the four groups were collected and the expression levels of soluble vascular cell adhesion molecule-1 (sVCAM-1),tumor necrosis factor-α(TNF-α),and T-lymphocyte subsets were detected.One-way analysis of variance,x^2 test and Pearson correlation were used to manage the data.Results:The prevalence of hypertension and coronary heart disease was significantly higher in the OS group than in the control,OSA,and COPD groups (x^2 =20.69,P < 0.05 and x^2 =11.03,P < 0.05,respectively).The levels of sVCAM-1 and TNF-α were significantly higher in the OS group than in other groups (F =127.40,P < 0.05 and F =846.77,P < 0.05,respectively).The percentage of CD4+ lymphocytes and CD4+/CD8+ were both significantly lower in the OS group than in any other group (F =25.40,P < 0.05 and F =75.08,P < 0.05,respectively).There were significantly negative correlations in the levels of sVCAM-1 and TNF-α with CD4^+/CD8^+ lymphocytes (r =-0.77,P < 0.05 and r =-0.83,P < 0.05,respectively).Conclusions:The prevalence of hypertension and coronary heart disease was higher in patients with OS than in patients with either OSA or COPD alone.Patients with OS exhibited more severe vascular endothelial injury,stronger inflammatory response,and lower cellular immune function.

Regulatory Effect of Langchuang Serial Recipes on T-Lymphocyte Subsets Th and Tc in Patients with Systemic Lupus Erythematosus

Objective： To study the principle of clearing Fei （肺）, cooling blood, and detoxification as well as nourishing yin and moisening Fei （abbr. as CCD-NM） in regulating the levels of peripheral T-lymphocyte subsets Th and Tc cells to explore its mechanism for lowering the incidence of infection in patients with systemic lupus erythematosus iSLE）. Methods： Sixty SLE patients without complicated infection were assigned to the treatment group and the control group, 30 in each group. The control group was treated with Western medicine alone, while the treatment group was treated with the same program of Western medicine, but additionally administered with either Langchuang No.1 （狼疮 Ⅰ号） or 2 （狼疮 Ⅱ 号）, serial concerted Chinese recipes, applied respectively in patients in the active stage or in the resting stage. The total time of treatment for both groups was 1 year. Further, a healthy control group was set up with 20 healthy subjects. The expressions of Thl, Th2, and Tcl and Tc2 cells in peripheral blood were detected and compared with those in the healthy control group. Results： （1） As compared with the healthy control group, ratios of Thl/Th2 and Tcl/Tc2 in SLE patients, whether complicated with infection or not, were significantly lower （P〈0.05 or P〈0.01）. （2） Comparison between patients with complications and those uncomplicated with infection showed that the two ratios and Thl expression were lower and Tc2 was higher in the former than those in the latter （all P〈0.05）. （3） Ratios of Thl/Th2 and Tcl/Tc2 increased after treatment in patients of both the treatment group and the control group （P〈0.05 and P〈0.01）, but the changes in the treatment group were more significant （P〈0.05）. Conclusion： The principle of CCD-NM could regulate the Th and Tc subsets toward equilibrium in SLE patients, which might be one of the mechanisms of action for alleviating complicated infection.

Clinical characteristics and T-lymphocyte subsets in 48 acquired immune deficiency syndrome patients with cytomegalovirus infections

Objective To investigate the clinical features and Tlymphocytes subsets in patients with acquired immunedeficiency syndrome ( AIDS ) and cytomegalovirus(CMV) infection. Methods A total of 48 hospitalizedpatients with human immunodeficiency virus (HIV)-1 /AIDS and CMV infections were recruited at Peking UnionMedical College Hospital from Jan 2010 to Aug 2017.Their clinical features and immune function were retrospectivelyanalyzed. Patients with only HIV/AIDS inprevious study were recruited as controls. Results All 48patients were at C3 stage, including 36 men and12 women. Five of them were younger than 30 years old,33 cases within 31 - 50 years old,and 10 cases olderthan 50 years old.

腺样体肥大患儿机体免疫功能变化研究

<正>腺样体也称咽扁桃体或增殖体,属于淋巴组织,表面呈桔瓣样。正常生理情况下,儿童6~7岁时腺样体发育至最大,青春期后逐渐萎缩,成人后基本消失~[1]。腺样体位于鼻咽顶后壁处,病理性肥大易引起鼻堵、张口呼吸、耳闷、听力下降等症状,最终造成慢性鼻-鼻窦炎、阻塞性睡眠呼吸暂停低通气综合征及分泌性中耳炎等疾病,给儿童的颌面骨发育、智力发育造成严重影响。

腹泻型肠易激综合征患者外周血T淋巴细胞亚群及血清微量元素含量变化研究

目的:通过测定腹泻型肠易激综合征(D-IBS)患者外周血T淋巴细胞亚群及血清Zn、Se、Fe、Cu含量变化,旨在探讨IBS的可能病因及发病新机制。方法:随机抽取30例D-IBS患者和30例健康志愿者的外周血标本,采用流式细胞技术,分别测定CD3、CD4、CD8T淋巴细胞亚群百分比,并计算CD4/CD8比值;采用原子吸收光谱法,测定血清Zn、Fe、Cu水平,用原子荧光分析法,测定血清Se水平。结果:D-IBS组外周血CD4T淋巴细胞水平及CD4/CD8比值较正常对照组明显降低(P<0.01);D-IBS组与正常组血清Zn、Se、Fe、Cu水平差异无统计学意义(P>0.05)。结论:外周血T淋巴细胞CD4水平及CD4/CD8比值降低提示D-IBS患者可能存在细胞免疫异常;D-IBS患者血清Zn、Se、Fe、Cu水平较正常对照组无统计学意义。

肝癌合并肝硬化患者脾脏T细胞亚群免疫状态的研究

目的 探讨肝癌患者脾脏T细胞亚群免疫状态。方法 采用流式细胞技术 (FCM )检测 3 1例肝癌患者外周及脾静脉血T细胞亚群CD 4+ ,CD 8+ ,CD4+ /CD8+ (以下简称CD 4,CD 8,CD 4/CD 8)的变化 ,并以 13例肝硬化患者作为对照组。结果 6例Ⅰ期肝癌患者脾静脉血CD 4高于肝硬化组 ,外周血CD 8低于肝硬化组 (P <0 .0 5 ) ,外周及脾静脉血CD 4/CD8高于肝硬化组 (P <0 .0 1)。 12例Ⅱ期、13例Ⅲ期肝癌患者外周及脾静脉血CD4,CD 4/CD8低于肝硬化组 ,CD 8高于肝硬化组 (P <0 .0 1或 P <0 .0 5 ) ,且脾静脉血CD4,CD 4/CD 8低于外周血 ,CD 8高于外周血 (P <0 .0 5 )。结论 早期肝癌患者 ,T细胞亚群处于良好抗肿瘤免疫状态 ,脾脏在维持机体T细胞亚群平衡中起重要作用。随肿瘤进展 ,机体免疫功能逐渐受损 ;中晚期肝癌患者T细胞亚群紊乱 ,机体处于免疫抑制状态 ,尤其以脾脏T细胞亚群紊乱更为明显 ,出现了负性抗肿瘤免疫作用 ,加重了机体免疫抑制。

自血穴注与捏脊疗法对恶性肿瘤患者细胞免疫的影响

对放化疗中患者的细胞免疫进行了分组观察 ,同时设增强免疫药物为对照组。结果表明 :两种方法均可提高 T细胞的总数。CD3和 E-RFC治疗后明显提高 ( P<0 .0 5) ;CD4 / CD8的比例较对照组显著改善 ( P<0 .0 1 ) ;捏脊组的淋巴细胞转变率明显提高 ( P<0 .0 1 )。

手术对淋巴细胞亚群细胞周期的影响

目的:观察实验动物围手术期脾脏淋巴细胞亚群细胞周期改变,分析手术对机体免疫功能的影响及其意义。方法:对Balb/c小鼠行截肢术,分别于术后第1、2、3、4d处死,分离脾脏淋巴细胞,单克隆荧光抗体标记,流式细胞检测各淋巴细胞亚群的细胞周期。结果:术后第1天CD3+、CD4+细胞的(S+G2)期明显增高,分别为43.5±3.4%和46.5±4.1%,维持至术后第4天。CD8+(S+G2)期术后第2天62.7±5.7%较对照组41.3±1.9%明显升高。sIG(膜表面免疫球蛋白)术后第2天(S+G2)期明显升高。

HCV侵染患者外周血单个核细胞后对细胞免疫功能的影响

目的探讨丙型病毒性肝炎（丙肝）患者外周血单个核细胞（ＰＢＭＣ）ＨＣＶＲＮＡ（＋）后对其细胞免疫功能的影响及其在丙肝发病机制中的作用。方法以ＲＴＰＣＲ法检测１３５例丙肝患者ＰＢＭＣ内ＨＣＶＲＮＡ，同时用生物素—链霉亲和素（ＢＳＡ）系统检测其Ｔ细胞亚群及经ＰＨＡ诱导前后膜白介素２受体（ｍＩＬ２Ｒ）的表达水平。结果丙肝患者与正常对照相比，ＣＤ＋３、ＣＤ＋４及ｍＩＬ２Ｒ表达水平降低，ＣＤ＋８水平增高，差异均有显著性（Ｐ＜００１）。ＰＢＭＣ内ＨＣＶＲＮＡ（＋）组与ＨＣＶＲＮＡ（－）组相比较，前者ＣＤ＋３、ＣＤ＋４百分率降低，ＣＤ＋８百分率增高，ＣＤ＋４／ＣＤ＋８比值下降均大于后者，差异有显著性（Ｐ＜００５）。ＰＨＡ诱导前后ＨＣＶＲＮＡ（＋）者明显低于ＨＣＶＲＮＡ（－）者，ｍＩＬ２Ｒ表达水平亦以患者低于正常对照（Ｐ＜００５）。结论丙肝患者细胞免疫功能低下，病毒侵染ＰＢＭＣ后可加重患者的细胞免疫功能紊乱，抑制ｍＩＬ２Ｒ表达，使病情迁延反复。检测丙肝患者ＰＢＭＣ内ＨＣＶＲＮＡ对揭示丙肝发病机制及其防治均有重要意义。

BSA系统检测结核病患者T细胞亚群和mIL-2R

目的研究各类结核病患者的细胞免疫功能表达。方法用生物素—链霉亲和素方法检测９６例肺结核、结核性胸膜炎、肾结核患者Ｔ细胞亚群ＣＤ３＋、ＣＤ４＋、ＣＤ８＋、ｍＩＬ－２Ｒ（膜白细胞介素－２受体）水平；另选取２６例正常人群为对照组。结果结核病患者与正常人相比，ＣＤ８＋明显升高，而ＣＤ３＋、ＣＤ４＋／ＣＤ８＋及ｍＩＬ－２Ｒ均显著降低（Ｐ＜０．０１），其中肺结核患者最为明显，有空洞者较无空洞者更明显（Ｐ＜０．０１）。无空洞型肺结核与结核性胸膜炎、肾结核的Ｔ细胞亚群、ｍＩＬ－２Ｒ表达水平相似，相互间差异无显著性（Ｐ＞０．０５）。结论结核病患者体内存在明显细胞免疫功能低下，病变部位、程度及预后与ＣＤ３＋、ＣＤ４＋、ＣＤ８＋、ｍＩＬ－２Ｒ水平有一定关系。

ITP患儿血清可溶性IL-2受体水平和T淋巴细胞亚群变化

探讨特发性血小板减少性紫癜（ＩＴＰ）患儿免疫功能变化。方法采用双抗体夹心ＥＬＩＳＡ法对ＩＴＰ患儿进行了血清可溶性白细胞介素２受体（ｓＩＬ－２Ｒ）测定和ＡＰＡＡＰ法检测Ｔ淋巴细胞亚群。结果ＩＴＰ患儿血清ｓＩＬ－２Ｒ、ＣＤ８显著升高，ＣＤ４／ＣＤ８显著下降（Ｐ＜０．０１），且ｓＩＬ－２Ｒ和ＣＤ４／ＣＤ８呈负相关（ｒ＝－０．５８１，Ｐ＜０．０１）；缓解期ｓＩＬ－２Ｒ明显下降，基本接近正常水平，而ＣＤ８、ＣＤ４／ＣＤ８比值较急性期变化不明显。

中药复感灵对环磷酰胺造型小鼠免疫功能的影响

观察了由党参、黄芪、白术、丹参、防风、贯众、大青叶、金银花等组成的自拟中药复感灵对小鼠免疫功能的影响。结果表明，复感灵能提高环磷酰胺造型小鼠Ｔ淋巴细胞和Ｂ淋巴细胞的转化率；对溶血空斑生成的影响。