Background Hemorrhagic shock induces immune dysfunction. Regulatory T cells （Tregs）, T-helper （Th） cells, and cytotoxic T-lymphocytes （CTLs） can execute many crucial actions in immune and inflammatory responses....Background Hemorrhagic shock induces immune dysfunction. Regulatory T cells （Tregs）, T-helper （Th） cells, and cytotoxic T-lymphocytes （CTLs） can execute many crucial actions in immune and inflammatory responses. This study was conducted to investigate the early pathophysiological changes of CD4＋CD25＋Foxp3＋ Treg and Th1/Th2, Tc1/Tc2 profiles in the peripheral blood of rats with controlled hemorrhagic shock and no fluid resuscitation. Methods A rat model of controlled hemorrhagic shock with no fluid resuscitation was established. Peripheral blood samples were taken before and four hours after hemorrhagic shock with no fluid resuscitation. Three color flow cytometry was used to detect Tregs, Thl, Th2, Tcl and Tc2 ceils in the samples. Results In the peripheral blood of rats, the percentage of Tregs four hours after hemorrhagic shock was significantly lower than before hemorrhagic shock （P=0.001）. The ratios of Th1/Th2 and Tc1/Tc2 were changed from （23.08±8.98）% to （23.91±15.36）%, and from （40.40±21.56）% to （65.48±23.88）%, respectively. Conclusions At an early stage, the advent of hemorrhagic shock is related to an early decrease of Tregs, and a mild shift in the Th1/Th2, Tc1/Tc2 balance toward Thl and Tcl dominance. These changes are part of a hyper-inflammatory state of the host, and will deteriorate the maintenance of immune balance. Further influences and detailed mechanisms need to be investigated.展开更多
OBJECTIVE:To evaluate the effectiveness of Xiaoyin Jiedu(XYJD)granules in the treatment of psoriasis vulgaris(PSV)in patients with a blood-heat pattern(BHP)in terms of Traditional Chinese Medicine(TCM).We also aimed t...OBJECTIVE:To evaluate the effectiveness of Xiaoyin Jiedu(XYJD)granules in the treatment of psoriasis vulgaris(PSV)in patients with a blood-heat pattern(BHP)in terms of Traditional Chinese Medicine(TCM).We also aimed to identify the possible underlying immunological mechanism.METHODS:Twenty-five PSV patients with BHP and ten normal controls were enrolled from January 1,2015 to December 31,2016.Patients were randomly assigned to either the XYJD group(15 cases)or the placebo group(10 cases),in which patients were treated with XYJD granules or a placebo,respectively.Additionally,albolene was used to relieve skin dryness in these two groups.The psoriasis area and severity indexes,dermatology life quality indexes and itching scores were assessed at the end of the 2 nd,4 th and 8 th week of treatment.The number of peripheral blood T helper(Th)9,Th17 and regulatory T cells(Tregs)and the m RNA and protein expression levels of PU.1,RAR-related orphan receptor(ROR)-γt,forkhead box protein 3(Foxp3),interleukin(IL)-9,IL-17,IL-23 and IL-10 in the control and experimental groups were compared before and after treatment.RESULTS:Psoriasis area and severity indexes and itching scores of patients in the XYJD group were significantly lower than those in the placebo group,whereas dermatology life quality indexes were significantly higher.In comparison with the placebo group,XYJD granules significantly reduced the number of Th17 cells and the m RNA and protein expression levels of Th17-related ROR-γt,IL-17,IL-22 and IL-23 in the peripheral blood and reduced the number of Th9 cells and the m RNA and protein expression levels of Th9-related PU.1 and IL-9.CONCLUSION:XYJD granules were effective against PSV in patients with BHP by reducing the number of Th9 and Th17 cells and the levels of their related cytokines.展开更多
Graft-versus-host disease （GVHD） is a severe complication and a major source of morbidity and mortality after allogeneic hematopoietic stem cell transplantation （HSCT）. Although T cell depletion of the allogeneic ...Graft-versus-host disease （GVHD） is a severe complication and a major source of morbidity and mortality after allogeneic hematopoietic stem cell transplantation （HSCT）. Although T cell depletion of the allogeneic HSCT can efficiently prevent GVHD, it is associated with increased graft rejection and relapse of the malignant disease. To preserve the beneficial effects of donor T cells and avoid their GVHD effects, some approaches have been explored. One of them is to transfer a special suicide gene into the donor T lymphocytes so that they become more sensitive to a specific drug that is ordinarily not toxic. The most commonly used suicide gene is the thymidine kinase-encoding gene of herpes simplex virus （HSV-tk）.展开更多
基金This work was supported by a grant from the Medical and Health Science Foundation of Zhejiang Province, China （No. 2009B052）.
文摘Background Hemorrhagic shock induces immune dysfunction. Regulatory T cells （Tregs）, T-helper （Th） cells, and cytotoxic T-lymphocytes （CTLs） can execute many crucial actions in immune and inflammatory responses. This study was conducted to investigate the early pathophysiological changes of CD4＋CD25＋Foxp3＋ Treg and Th1/Th2, Tc1/Tc2 profiles in the peripheral blood of rats with controlled hemorrhagic shock and no fluid resuscitation. Methods A rat model of controlled hemorrhagic shock with no fluid resuscitation was established. Peripheral blood samples were taken before and four hours after hemorrhagic shock with no fluid resuscitation. Three color flow cytometry was used to detect Tregs, Thl, Th2, Tcl and Tc2 ceils in the samples. Results In the peripheral blood of rats, the percentage of Tregs four hours after hemorrhagic shock was significantly lower than before hemorrhagic shock （P=0.001）. The ratios of Th1/Th2 and Tc1/Tc2 were changed from （23.08±8.98）% to （23.91±15.36）%, and from （40.40±21.56）% to （65.48±23.88）%, respectively. Conclusions At an early stage, the advent of hemorrhagic shock is related to an early decrease of Tregs, and a mild shift in the Th1/Th2, Tc1/Tc2 balance toward Thl and Tcl dominance. These changes are part of a hyper-inflammatory state of the host, and will deteriorate the maintenance of immune balance. Further influences and detailed mechanisms need to be investigated.
基金Supported by grants from the Chinese National Natural Science Foundation Effect and Action of Cooling Blood and Removing Toxins Traditional Chinese Medicine on New T Cells Subsets and Related Cytokines in Patients with Psoriasis Vulgaris(No.81403403)Immunological Mechanisms Research of Psoriasis Discuss of Traditional Chinese Medicine Based on Imbalance Theory of Helper T Cell(Th)1/Th2,Th17/Regulatory T Cells(Tregs)(No.81403404)+2 种基金The Study on the Relationship Between New T Helper Cells Subsets Th17,Th22,Effect Factor Interleukin(IL)-22 and Syndrome Differentiation Treatment of Psoriasis(No.81273755)2016 Beijing University of Chinese Medicine Foundation for Distinguished Young Scholars Study on The Mechanism of Cooling Blood Detoxification on Different Histopathological Levels of Psoriasis(No.2016-JYB-XJQ005)Young Scientist Development Program 2016,Dong Zhimen Hospital,Affiliated to Beijing University of Chinese Medicine(No.DZMYS-201603)
文摘OBJECTIVE:To evaluate the effectiveness of Xiaoyin Jiedu(XYJD)granules in the treatment of psoriasis vulgaris(PSV)in patients with a blood-heat pattern(BHP)in terms of Traditional Chinese Medicine(TCM).We also aimed to identify the possible underlying immunological mechanism.METHODS:Twenty-five PSV patients with BHP and ten normal controls were enrolled from January 1,2015 to December 31,2016.Patients were randomly assigned to either the XYJD group(15 cases)or the placebo group(10 cases),in which patients were treated with XYJD granules or a placebo,respectively.Additionally,albolene was used to relieve skin dryness in these two groups.The psoriasis area and severity indexes,dermatology life quality indexes and itching scores were assessed at the end of the 2 nd,4 th and 8 th week of treatment.The number of peripheral blood T helper(Th)9,Th17 and regulatory T cells(Tregs)and the m RNA and protein expression levels of PU.1,RAR-related orphan receptor(ROR)-γt,forkhead box protein 3(Foxp3),interleukin(IL)-9,IL-17,IL-23 and IL-10 in the control and experimental groups were compared before and after treatment.RESULTS:Psoriasis area and severity indexes and itching scores of patients in the XYJD group were significantly lower than those in the placebo group,whereas dermatology life quality indexes were significantly higher.In comparison with the placebo group,XYJD granules significantly reduced the number of Th17 cells and the m RNA and protein expression levels of Th17-related ROR-γt,IL-17,IL-22 and IL-23 in the peripheral blood and reduced the number of Th9 cells and the m RNA and protein expression levels of Th9-related PU.1 and IL-9.CONCLUSION:XYJD granules were effective against PSV in patients with BHP by reducing the number of Th9 and Th17 cells and the levels of their related cytokines.
文摘Graft-versus-host disease （GVHD） is a severe complication and a major source of morbidity and mortality after allogeneic hematopoietic stem cell transplantation （HSCT）. Although T cell depletion of the allogeneic HSCT can efficiently prevent GVHD, it is associated with increased graft rejection and relapse of the malignant disease. To preserve the beneficial effects of donor T cells and avoid their GVHD effects, some approaches have been explored. One of them is to transfer a special suicide gene into the donor T lymphocytes so that they become more sensitive to a specific drug that is ordinarily not toxic. The most commonly used suicide gene is the thymidine kinase-encoding gene of herpes simplex virus （HSV-tk）.
