目的:探讨SPOC+TBIM合Sim Man 3G在急诊科住院医师规范化培训中的应用效果。方法:选取轮转至急诊科接受住院医师规范化培训的40人,随机分为实验组和对照组,每组20人,实验组采用SPOC+TBLM合Sim Man 3G教学,对照组采用传统教学。教学结束...目的:探讨SPOC+TBIM合Sim Man 3G在急诊科住院医师规范化培训中的应用效果。方法:选取轮转至急诊科接受住院医师规范化培训的40人,随机分为实验组和对照组,每组20人,实验组采用SPOC+TBLM合Sim Man 3G教学,对照组采用传统教学。教学结束后,比较两组学生的出科考核成绩及问卷调查满意度,评价不同教学模式的教学效果。结果:实验组学员的疾病诊治能力、临床技能的出科考核及教学评价均优于采取传统规培教学模式培养的学员(P<0.05)。结论:SPOC+TBIM合Sim Man 3G教学法引入急诊科住院医师规范化培训中可以激发学员的学习兴趣,显著提高了学员临床的思维能力,规培学员满意度较高,这为急诊科住院医师规范化培训实践提供了新的思路和模式。展开更多
Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard D...Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard Diagnostics) Bio line, South Korea was evaluated. The ICT is a rapid, reliable and cheaper method that can be used instead of conventional biochemical tests for confirming MTB in culture isolates in resource limited laboratories. The study also evaluated the ability of ICT to detect MPT64-Antigen before the micro MGIT could signal positive. Material/Methods: A total of 450 sputum samples of individual patients were used for the study. 152 isolates of Mycobacteria were recovered from solid and liquid media. These strains were tested for the detection of MPT64-antigen. H37Rv strain was served as the positive reference control and also used for early detection of Antigen experiment. Findings: The development of bands on both test and sample region when H37Rv strain was tested were seen (MPT64 antigen positive). When 138 MTB isolates were tested, it showed a similar banding pattern indicating 100% sensitivity. MPT64 band formation was not detected in any of the 14 isolates indicating 100% specificity. Both PPV & NPV were 100%. All the isolates negative for MPT64 Ag were confirmed as MOTT by conventional bio-chemical PNBA. The H37Rv strain showed a faint band from the 2nd day onwards from inoculation till 3rd day in the earlier Antigen detection experiment. Conclusion: Rapid identification of MTB culture isolate is a pressing need for diagnosis and proceeding to perform drug susceptibility testing. MPT64 TB Ag detection ICT kit is a rapid, reliable method, good substitute for molecular identification methods, and conventional biochemical test which is time-consuming and technically demanding. The early detection of Antigen can be used as an effective tool in diagnosis.展开更多
文摘目的:探讨SPOC+TBIM合Sim Man 3G在急诊科住院医师规范化培训中的应用效果。方法:选取轮转至急诊科接受住院医师规范化培训的40人,随机分为实验组和对照组,每组20人,实验组采用SPOC+TBLM合Sim Man 3G教学,对照组采用传统教学。教学结束后,比较两组学生的出科考核成绩及问卷调查满意度,评价不同教学模式的教学效果。结果:实验组学员的疾病诊治能力、临床技能的出科考核及教学评价均优于采取传统规培教学模式培养的学员(P<0.05)。结论:SPOC+TBIM合Sim Man 3G教学法引入急诊科住院医师规范化培训中可以激发学员的学习兴趣,显著提高了学员临床的思维能力,规培学员满意度较高,这为急诊科住院医师规范化培训实践提供了新的思路和模式。
文摘Background: A new rapid Immunochromatographic test (ICT) kit (MPT64 TB Ag Kit) for detection of MPT64 Antigen in M. tuberculosis (MTB) isolates used for rapid identification of MTB isolates developed by SD (Standard Diagnostics) Bio line, South Korea was evaluated. The ICT is a rapid, reliable and cheaper method that can be used instead of conventional biochemical tests for confirming MTB in culture isolates in resource limited laboratories. The study also evaluated the ability of ICT to detect MPT64-Antigen before the micro MGIT could signal positive. Material/Methods: A total of 450 sputum samples of individual patients were used for the study. 152 isolates of Mycobacteria were recovered from solid and liquid media. These strains were tested for the detection of MPT64-antigen. H37Rv strain was served as the positive reference control and also used for early detection of Antigen experiment. Findings: The development of bands on both test and sample region when H37Rv strain was tested were seen (MPT64 antigen positive). When 138 MTB isolates were tested, it showed a similar banding pattern indicating 100% sensitivity. MPT64 band formation was not detected in any of the 14 isolates indicating 100% specificity. Both PPV & NPV were 100%. All the isolates negative for MPT64 Ag were confirmed as MOTT by conventional bio-chemical PNBA. The H37Rv strain showed a faint band from the 2nd day onwards from inoculation till 3rd day in the earlier Antigen detection experiment. Conclusion: Rapid identification of MTB culture isolate is a pressing need for diagnosis and proceeding to perform drug susceptibility testing. MPT64 TB Ag detection ICT kit is a rapid, reliable method, good substitute for molecular identification methods, and conventional biochemical test which is time-consuming and technically demanding. The early detection of Antigen can be used as an effective tool in diagnosis.
