Objective: To investigate the distribution and clonality of TCR Vα subfamily T cells in patients with acute promyelocytic leukemia (APL). Methods: The complementary determining region 3 (CDR3) of TCR Vα 29 subfamily...Objective: To investigate the distribution and clonality of TCR Vα subfamily T cells in patients with acute promyelocytic leukemia (APL). Methods: The complementary determining region 3 (CDR3) of TCR Vα 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were amplified using RT-PCR. The positive products were further analyzed to identify the clonality of T cells by GeneScan technique. Results: One to seven of TCR Vα subfamilies could be detected in peripheral blood T cells from 9 cases with APL, the frequent expression of Vα subfamilies predominated in Vα3 and Vα19. Clonal expanded T cells could be detected in 8 APL patients, which predominant used Vα3, Vα26 or Vα27 (3 out of 8 cases). However, almost all Vα subfamilies with polyclonal expansion could be detected in peripheral blood T cells from 10 cases of normal individuals. Conclusion: Remarkable skew distribution and clonal expansion of TCR Vα subfamilies T cells is the common feature in patients with APL. Clonal expansion of T cells might reflect a response in host to APL cell as- sociated antigen, whether these expanded T cells have the ability for specific cytotoxicity against APL cells, remains an open question.展开更多
基金Supported by grants from National Natural Science Foundation of China (No. 39870358)Natural Science Foundation of Guangdong Province (No. 2005B50301016).
文摘Objective: To investigate the distribution and clonality of TCR Vα subfamily T cells in patients with acute promyelocytic leukemia (APL). Methods: The complementary determining region 3 (CDR3) of TCR Vα 29 subfamily genes in peripheral blood mononuclear cells from 9 APL patients were amplified using RT-PCR. The positive products were further analyzed to identify the clonality of T cells by GeneScan technique. Results: One to seven of TCR Vα subfamilies could be detected in peripheral blood T cells from 9 cases with APL, the frequent expression of Vα subfamilies predominated in Vα3 and Vα19. Clonal expanded T cells could be detected in 8 APL patients, which predominant used Vα3, Vα26 or Vα27 (3 out of 8 cases). However, almost all Vα subfamilies with polyclonal expansion could be detected in peripheral blood T cells from 10 cases of normal individuals. Conclusion: Remarkable skew distribution and clonal expansion of TCR Vα subfamilies T cells is the common feature in patients with APL. Clonal expansion of T cells might reflect a response in host to APL cell as- sociated antigen, whether these expanded T cells have the ability for specific cytotoxicity against APL cells, remains an open question.
