期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Detection of Immunoglobulin Heavy China and T Cell Receptorγ Gene Rearrangement in Lymph Node Aspirates with PCR:Diagnostic Significance for Lymphoproliferative Disorders
1
作者 盛瑞兰 《The Journal of Biomedical Research》 CAS 1994年第1期49-52,共4页
Lymph node aspirates of 17 cases with enlarged superficial or isceral lymph nodes were detected for immunoglobulin heavy chain gene rearrangement (IgHRA) and T cell rcceptor γ gene rearrangement (TCRγRA) bypolymeras... Lymph node aspirates of 17 cases with enlarged superficial or isceral lymph nodes were detected for immunoglobulin heavy chain gene rearrangement (IgHRA) and T cell rcceptor γ gene rearrangement (TCRγRA) bypolymerase chain reaction (PCR). Combining with clinical data, pathologic diagnosis and immunophenotapy, we analyse the results as follows: 5 cases with nonlymphoid cancers and 3 cases with reactive lymphadenopathy do not present two kinds of clone gene rearrangements.5 out of 7 cases with NHL show clone gene rearrangements (IgH 3 cases, TCRY 2cases),two kinds of monoclonal band(100-120bp for IgHRA and 170-230bp for TCRγRA) were observed after electrophoresis of amplified DNA products. One case whose clinical sitcaion accorded with features of lymphoma was diagnosed as granulomatous lymphadenitis by pathologist, after gene rearaangement clone TCRγRA was detected,a correct diaguosis as NHL was made then. The significance of detecting the two kinds of gene rearrangement for clinical application and the limitations in diagnosis of lymphoproliferative disorders was discussed. 展开更多
关键词 PCR IgH and tcrγ gene rearrangement lymph node asprate lymphoproliferative Disorders
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部