[Objective]In order to carriy on effective medication and immune prevention and control,pathogen and etiology of a Rana spinosa David disease in a Rana spinosa David breeding company in Zhejiang was researched from 20...[Objective]In order to carriy on effective medication and immune prevention and control,pathogen and etiology of a Rana spinosa David disease in a Rana spinosa David breeding company in Zhejiang was researched from 2009 to 2010. [Methods]Conventional bacteria separation and purification technology was used to purify pathogenic bacteria,regression infection and toxicity test was used to determine its virulence,physiological and biochemical and molecular biology identification was carried out to the the pathogen,k- b paper method was adopted for the susceptibility test,normal paraffin wax flaking technology was used for pathology observations,finally,the inactivation conditions of pathogenic bacteria was explored. [Results]The pathogenic bacteria separated from sick Rana spinosa David body had Median Lethal Concentration（ LC 50） of 5.62 ×105 cfu / ml; the strain was identified as Aeromonas hydrophila by ATB bacteria identification instrument and 16 s rRNA sequence analysis（ GenBank login number： HQ322682）; histopathology observation results showed that the disease had main symptoms of the liver and kidney damage,inflammatory cells increase. Susceptibility test showed that the strain was highly sensitive to gentamicin,norfloxacin,florfenicol,enrofloxacin etc. [Conclusions]1% formaldehyde could well inactivate the pathogenic bacteria at 60 ℃ for 24 h,which would provide technical reference for whole bacteria inactivated vaccine preparation.展开更多
Neuromuscular disorders are commonly encountered in neurological practices. They may lead tosignificant disability or death. The accurate and early diagnosis of a cause is very important for the effective treatment an...Neuromuscular disorders are commonly encountered in neurological practices. They may lead tosignificant disability or death. The accurate and early diagnosis of a cause is very important for the effective treatment and reduction in the disability and improved quality of life. A comprehensive history and physical examination is essential to aid classification of the neuromuscular disorder. In some instances, the diagnosis of a specific neuromuscular condition can be made entirely on clinical grounds, such as a patient with carpal tunnel syndrome, while in many cases the presenting symptoms are nonspecific and could be the result of several possible etiologies, such as a patient with generalized weakness that may be the result of a myopathy, neuromuscular junction disorder, peripheral neuropathy, or motor neuron disease. Therefore,展开更多
基金Supported by Special Funds(NYCYTX-49-17) of Modern Agriculture Industry Technology System ConstructionOpen Issues(BM2007-07) of Aquatic Animal Genetics,Breeding and Breeding Biology Key Open Laboratory,the Ministry of AgricultureScientific Research Project of Ocean Public Welfare Industry of State Oceanic Administration,China(201105007-7)
文摘[Objective]In order to carriy on effective medication and immune prevention and control,pathogen and etiology of a Rana spinosa David disease in a Rana spinosa David breeding company in Zhejiang was researched from 2009 to 2010. [Methods]Conventional bacteria separation and purification technology was used to purify pathogenic bacteria,regression infection and toxicity test was used to determine its virulence,physiological and biochemical and molecular biology identification was carried out to the the pathogen,k- b paper method was adopted for the susceptibility test,normal paraffin wax flaking technology was used for pathology observations,finally,the inactivation conditions of pathogenic bacteria was explored. [Results]The pathogenic bacteria separated from sick Rana spinosa David body had Median Lethal Concentration（ LC 50） of 5.62 ×105 cfu / ml; the strain was identified as Aeromonas hydrophila by ATB bacteria identification instrument and 16 s rRNA sequence analysis（ GenBank login number： HQ322682）; histopathology observation results showed that the disease had main symptoms of the liver and kidney damage,inflammatory cells increase. Susceptibility test showed that the strain was highly sensitive to gentamicin,norfloxacin,florfenicol,enrofloxacin etc. [Conclusions]1% formaldehyde could well inactivate the pathogenic bacteria at 60 ℃ for 24 h,which would provide technical reference for whole bacteria inactivated vaccine preparation.
文摘Neuromuscular disorders are commonly encountered in neurological practices. They may lead tosignificant disability or death. The accurate and early diagnosis of a cause is very important for the effective treatment and reduction in the disability and improved quality of life. A comprehensive history and physical examination is essential to aid classification of the neuromuscular disorder. In some instances, the diagnosis of a specific neuromuscular condition can be made entirely on clinical grounds, such as a patient with carpal tunnel syndrome, while in many cases the presenting symptoms are nonspecific and could be the result of several possible etiologies, such as a patient with generalized weakness that may be the result of a myopathy, neuromuscular junction disorder, peripheral neuropathy, or motor neuron disease. Therefore,
