<i>ADRBD1</i>(GRK2), <i>TBXA2R</i>and <i>VEGFA</i>rSNPs in KLF4 and SP1 TFBS Exhibit Linkage Disequilibrium

The adrenergic receptor kinase 1 (ADRBK1), thromboxane A2 receptor (TBXA2R) and vascular endothelial growth factor (VEGFA) regulatory (r) single nucleotide polymorphisms (SNPs) found in the potential stimulating protein-1 (SP1) and Kruppel-like factor-4 (KLF4) transcriptional factor binding sites (TFBS) within these genes are in linkage disequilibrium (LD). The LD may result from rSNP alleles that create TFBS for the KLF4 and SP1 transcriptional factors (TF) since the alternate rSNP alleles do not create these TFBS. Consequently, haplotypes carrying the rSNP alleles that create KLF4 and SP1 TFBS are essential for ADRBK1, TBXA2R and VEGFA gene regulation by these TFs.

SNPs and TFBS Associated with High Altitude Sickness

The rSNPs for the genes AKT3 (rs4590656), EGLN1 (rs480902), eNOS3 (rs1007311), and VEGFA (rs699947, rs13207311, rs1570360, rs2010963) have been significantly associated with the physiological parameters in high altitude sickness Han or Tibetan Chinese patients at the Qinghai-Tibetan plateau. The alleles of each rSNP have been found to create unique transcriptional factor binding sites for transcription factors that affect the process of hypoxia gene expression in this high altitude hypoxia environment.

基于碱基关联二联体位置权重矩阵预测酵母转录因子结合位点

基于已知的酵母转录因子结合位点数据资料,构建转录因子结合位点碱基关联二联体位置权重矩阵,整合碱基关联二联体位置权重矩阵和碱基保守性参量M2i,提出一种新的预测转录因子结合位点的方法(PWMSA).利用self-consistency和cross-validation两种方法对此算法进行检验,均获得了较高的预测成功率,结果表明9种转录因子结合位点的总体预测成功率超过81%,明显高于单碱基位置权重矩阵,同时与已有预测转录因子结合位点的软件进行比较,核苷酸水平上的关联系数和结合位点水平上的关联系数分别达到0.42和0.52,优于现有预测方法.

基于位点保守性参量和位置权重矩阵预测酵母转录因子结合位点

目的:改进转录因子结合位点的理论预测方法。方法:构建转录因子结合位点位置权重矩阵,以转录因子结合位点每一位置的碱基保守性指数Mi为参量,利用位置权重打分函数算法(PWMSA)对酵母五种转录因子结合位点进行预测。结果:利用self-consistency和cross-validation两种方法对此算法进行检验,均获得了较高的预测成功率,结果表明5种转录因子结合位点的预测成功率均超过80%。结论:与已有的三种预测转录因子结合位点的软件进行比较,PWMSA算法明显优于其他三种算法,核苷酸水平上的关联系数和结合位点水平上的关联系数分别提高了0.25和0.22。

遗传算法在转录因子结合位点识别中的应用

遗传算法是模拟生物进化过程的计算模型,是一种全局优化搜索算法。将遗传算法与转录因子结合位点识别问题相结合的新方法,以一致性序列模型作为保守motif的描述模型,通过对motif序列与待测序列的比对问题进行编码,将其转化成搜索空间中的优化问题,利用遗传算法来搜索最优解,预测转录因子的结合位点。实验结果表明,这种新的方法是有效的,它在占用少量内存的情况下能够准确地识别出待测转录因子结合位点。

不同类型生态浮床对富营养河水脱氮效果及微生物菌群的影响

通过设施大棚内容积为1.5 m3的人工模拟池试验,研究了框式与传统旱伞草浮床对富营养河水氮素转化及微生物菌群的影响.传统浮床是以聚乙烯泡沫板为载体,栽植陆生植物来削减水体氮磷和有机物质等,从而达到净化水质的效果.框式浮床是以塑料镂空支架为载体,除种植陆生植物外还添加填料等组件的新型浮床.结果表明:①2种浮床对水体中TN,NH4+-N和NO3--N均有显著的去除效果,其中框式浮床和传统浮床的NH4+-N去除率分别高达91%和86%,TN去除率也分别达到74%和64%,NO3--N去除率分别为49%和31%.②2种浮床系统有效地提高了水体中微生物和氮循环细菌总数和种群数量,尤其是框式浮床不同时期均比空白对照高出2～3个数量级.③氮循环细菌的数量跟水体氮素去除有显著相关性.其中水体ρ(NH4+-N)和氨化菌数量呈显著正相关,ρ(NH4+-N)和硝化菌数量呈极显著负相关,ρ(NO3--N),ρ(TN)和反硝化菌数量之间呈极显著负相关.④框式浮床的独特结构使之比传统浮床的去氮能力更强.其中,填料系统吸附贡献率为8%,植物吸收的去氮贡献为16.5%,微生物系统脱氮则为75.5%;而传统浮床植物系统吸收贡献率为31.8%,微生物系统脱氮贡献率为68.2%.说明浮床系统中植物吸收只是系统去氮的一种途径,微生物脱氮在2种浮床脱氮途径中占主导作用.

全固态薄膜锂电池研究进展

同锂离子电池相比,全固态锂电池不仅安全性好,而且在提高比能量、比功率密度以及循环性能方面也有更大的空间,从而得到广泛关注。在现有全固态锂电池中全固态薄膜锂电池(TFB)的制备工艺成熟,电池性能优异,已率先实现了商品化生产。同传统的锂离子电池不同,TFB的主要制备方法是物理成膜形成致密正极、电解质和负极薄膜,各层薄膜采用原位叠加方式形成。本文总结了近十年来TFB的研究工作,力图囊括全固态薄膜电池的完整制备过程以及各制备环节的技术进展和存在的科学技术问题。本文首先分述了固态电解质薄膜、正极薄膜、负极薄膜等三个主要构成部分的研究进展和关键问题,在此基础上,归纳了电极/电解质界面的设计、制备以及TFB制备过程及其关键问题和技术的研究进展,最后还介绍了基底、集流体、封装三个辅助部分的制备过程以及最近报道的新型特殊结构TFB。

新霉素致损小鼠听觉上皮后线粒体转录因子B1与毛细胞凋亡的关系

目的研究新霉素致损小鼠听觉上皮后线粒体转录因子B1（mitochodnrial transcription factor B1,TFB1M）与听觉上皮毛细胞凋亡的关系。方法每次解剖出20只出生1~2 d的新生ICR小鼠40个耳蜗,使用体外无血清培养法培养听觉上皮,培养后分为正常对照组和新霉素致损组（n=20）,然后在新霉素致损4、12、24 h及24 h洗脱后2 d检测TFB1M在听觉上皮细胞核和线粒体中的表达,以及听觉上皮线粒体膜电位的变化和毛细胞的凋亡。结果新霉素致损4 h后,听觉上皮中圈毛细胞形态完整,其线粒体膜电位开始降低;致损12 h,耳蜗听觉上皮线粒体以及细胞核内的TFB1M表达上调,听觉上皮出现毛细胞底圈、中圈大量毛细胞缺失,未缺失处毛细胞出现线粒体膜电位明显降低;致损24 h中圈、底圈毛细胞基本消失,未检测到线粒体膜电位。洗脱后2 d,TFB1M表达较正常对照组降低,基底膜毛细胞未见进一步缺失。而正常对照组在新霉素致损组相对应的时间点上,离体培养的听觉上皮的毛细胞形态完整,排列整齐,线粒体膜电位无改变。新霉素致损12 h,TFB1M在线粒体和细胞核内的表达较4、24 h,洗脱后2 d都明显（P〈0.01）。结论 TFB1M蛋白质水平的表达上调可能导致线粒体功能异常和毛细胞凋亡。

基于聚类和WOA的并行支持向量机算法

针对并行SVM在大数据环境下对冗余数据敏感、参数寻优能力差以及并行过程中出现的负载不均衡等问题,提出了一种基于聚类算法和鲸鱼优化算法的并行支持向量机算法MR-KWSVM。首先,该算法提出KF策略来删减冗余数据,利用删减冗余数据后的数据集训练SVM,降低SVM对冗余数据的敏感性;其次,提出了基于非线性收敛因子和自适应惯性权重的鲸鱼智能优化算法IW-BNAW,利用“IW-BNAW”算法获取SVM的最优参数,提高支持向量机的参数寻优能力;最后,在利用MapReduce构造并行SVM的过程中,提出时间反馈策略用于reduce节点的负载调度,提高了集群的并行效率,实现了高并行的SVM。实验结果表明,所提算法不仅保证了SVM在大数据环境下的高并行计算能力,SVM的分类准确度也有明显提高,并且具有更好的泛化性能。

磷矿石浮选精矿沉降试验研究

通过对磷矿石浮选精矿在清水和回水中进行沉降试验,研究了产品沉降的影响因素,结果表明,添加絮凝剂TFB能有效提高沉降效果,矿浆pH值及水质对沉降也有一定影响。

Z曲线在基因组中应用的研究与展望

随着后基因组时代的到来,大规模分子数据的处理和分析的需求变得越来越重要,生物数据的处理方法也层出不穷。这里介绍了一种新的表示DNA序列的模型—Z曲线,并详细的介绍了它的数学原理、和它在识别基因、基因起始位点以及识别Isochore结构上的应用,最后展望了Z曲线在识别TFBS上的应用。

ATF3 rSNPs, transcriptional factor binding sites and human etiology

Three rSNPs (rs3125289, rs1877474 and rs11119982) in one intron of the activating transcription factor 3 (ATF3) gene have been significantly associated with the human etiology of hypospadias and may be associated with human disease. These rSNP alleles alter the DNA landscape for potential transcriptional factors (TFs) to attach resulting in changes in transcriptional factor binding sites (TFBS). These TFBS changes are examined with respect to the human etiology of hypospadias which has been found to be significantly associated with the rSNPs.

TBXA2R rSNPs, Transcriptional Factor Binding Sites and Asthma in Asians

Four regulatory single nucleotide polymorphisms (rSNPs) (rs2238631, rs2238632, rs2238633 and rs2238634) in intron one, two rSNPs (rs1131882 and rs4523) in exon 3 and one rSNP (rs5756) in the 3’UTR of the thromboxane A2 receptor (TBXA2R) gene have been associated with childhood-onset asthma in Asians. These rSNP alleles alter the DNA landscape for potential transcriptional factors (TFs) to attach resulting in changes in transcriptional factor binding sites (TFBS). These TFBS changes are examined with respect to asthma which has been found to be significantly associated with the rSNPs.

红外收发模块在家用水表中的应用

为了实现远程获取水表系统中的信息,提出了使用红外收发模块远程通信的设计方案,并完成系统的软硬件设计。硬件中红外部分采用串行红外收发模块TFBS6614及其调制解调模块TOIM4232,软件采用ICC AVR编程,能够得到正确的信号波形。实际应用表明,该系统具有操作简便、通信准确的特点,达到了设计要求。

AKT3 rSNPs, Transcriptional Factor Binding Sites and Human Disease

Seven rSNPs (rs10157763, rs10927067, rs12031994, rs2125230, rs2345994, rs4132509 and rs4590646) in intron one of thev-akt murine thymoma viral oncogene homolog 3 (AKT3) gene have been significantly associated with either Chronic Mountain Sickness, Renal Cell Carcinoma risk or Aggressive Prostate Cancer. These rSNP alleles alter the DNA landscape for potential transcriptional factors (TFs)?to attach, resulting in changes in transcriptional factor binding sites (TFBS). The alleles of each rSNP were found to produce unique TFBS resulting in potential changes in TF AKT3 regulation. These regulatory changes are discussed with respect to the three diseases.

Identifying Changes in Punitive Transcriptional Factor Binding Sites Created by PPAR<i>α/δ/γ</i>SNPs Associated with Disease

Single nucleotide polymorphisms (SNPs) located in the PPARα/δ/γ genes that have been previously found to be significantly associated with human disease or a condition were also found to alter the genes punitive transcriptional factor binding sites (TFBS). Two alleles (C/G) of the PPARα SNP (rs1800206) were found to generate 7 common and 8 unique punitive TFBS. One of the unique TFBS created by the minor G (0.02) allele is for the T-Box 4 (TBX4) transcription factor which is associated with heritable pulmonary arterial hypertension. Two alleles (A/G) of the PPARδ SNP (rs2016520) were found to generate 20 unique punitive TFBS while the two alleles (C/G) of the PPARδ SNP (rs9794) were found to generate 11 common and 11unique punitive TFBS. The alleles of the PPARγ SNPs (rs10865710, rs12629751, rs709158, rs1805192 and rs3856806) were found to generate 15, 12, 16, 2 and 21 common and 9, 4, 12, 4 and 7 unique punitive TFBS, respectively. These changes in TFBS are discussed with relation to alterations in gene expression that may result in disease or change in human condition.

线粒体DNA的转录机制

真核生物的基因由核基因和线粒体基因(mt DNA)共同组成,mt DNA的生物合成是一个非常复杂的过程,需要核基因和mt DNA共同协同调节完成,大多线粒体蛋白在由核基因编码好后经特殊通道输送到线粒体内参与线粒体的各项生物合成。线粒体DNA转录需要核基因编码的几种蛋白,包括一个线粒体RNA聚合酶(POLRMT),线粒体转录因子A(TFAM),线粒体转录因子B1(TFB1M)或者线粒体转录因子B2(TFB2M)两者之一。本文对哺乳动物线粒体转录各因子的功能、各因子间的相互作用以及线粒体转录的调节进行了综述。

新品之窗·光电器件

LTC5100:激光驱动器 凌特公司推出高度集成的3.2Gbps垂直腔表面发射激光器二极管驱动器,用于中短距离光通信。LTC5100具有清洁、低噪声的传输眼图。LTC5100专为千兆位以太网与光纤通道光链路、多速率中距离(小于20公里)和短距离光纤数据链路、短距离SO-NET链路以及短途背板连接系统而设计。 LTC5100的数字控制器能够容纳很宽的VCSEL激光操作特性。用户可以设定所需的激光偏置电流、调制电流、激光功率、峰值、特定激光二极管的一阶和二阶温度系数等。

The key residues of OsTFIIAγ5/Xa5 protein captured by the argininerich TFB domain of TALEs compromising rice susceptibility and bacterial pathogenicity

Xanthomonas bacteria secrete transcription activator-like effector(TALE)proteins into host cells to activate plant disease susceptibility genes to cause disease,and the process is dependent on interaction between bacteria TFB domain of TALEs and host plant basal transcription factor IIA gamma subunit(TFIIAγ).The key domain or residues of plant TFIIAγand core residues of bacteria TFB domain that are indispensable for TFIIAγ-TALEs interaction in the process of TALE-carrying Xanthomonas invasion plants are unknown.Here,we showed that the thirdα-helix domain of OsTFIIAγ5/Xa5,especially the 38th,39th,40th and 42th residues were key sites for capturing by TALEs of Xanthomonas oryzae pv.oryzae(Xoo),the causal agent of rice bacterial blight disease.The latter segment of Xoo TFB domain harboring seventy-two amino acid residues was vital for TALE specific binding with host plant OsTFIIAγ5/Xa5.Substitution of some residues in this core region of TFB domain completely compromised capacity of TALEs capturing rice OsTFIIAγ5/Xa5.The rich and conserved arginine residues in this core region of TFB domain were responsible for TALE-dependent plant susceptibility gene activation and virulence of Xoo.These results provide a potential strategy for improving resistance to TALE-carrying pathogens in plants by site-specific modification of key residues of host plant TFIIAγ.

TFB1M在结直肠癌中的表达及意义

目的探讨线粒体转录因子B1(mitochondrial transcription factor B1,TFB1M)在结直肠癌组织中的表达及其意义。方法收集112例结直肠癌患者手术切除样本及临床信息,采用免疫组化法检测结直肠癌组织及相应癌旁正常组织中TFB1M的表达,并下载癌症基因组图谱(the Cancer Genome Atlas,TCGA)公共数据库中结直肠癌患者转录组数据及临床信息,分析TFB1M表达与患者临床病理特征及预后的关系。在结直肠癌SW620和HCT116细胞株中干涉及过表达TFB1M后,采用MTS检测细胞增殖能力。结果TFB1M在结直肠癌组织中阳性表达评分显著低于癌旁正常组织[(3.79±1.66)分vs(8.14±1.82)分,P<0.01]。TCGA数据和临床样本信息分析结果显示,TFB1M在Ⅲ+Ⅳ期患者中的表达量均较Ⅰ+Ⅱ期患者显著降低(P<0.01),TFB1M高表达的结直肠癌患者总生存期均优于低表达患者(P<0.05),且TFB1M是影响结直肠癌患者总生存期的独立因素。干涉TFB1M表达后,结直肠癌SW620和HCT116细胞增殖能力均较对照组显著增强(P<0.001);而过表达TFB1M细胞增殖能力均下降P<0.001。结论TFB1M低表达患者预后较差,干涉TFB1M可促进结直肠癌细胞增殖,TFB1M可能作为结直肠癌患者预后预测的生物标志物。