目的:探讨早期预见性护理干预在股骨近端钉系统(TFNA)手术中的应用效果。方法:选取广东省清远市中医院2020年1月—2021年1月收治的72例股骨粗隆骨折行TFNA手术患者,根据护理方式不同将其分为观察组(n=36)与对照组(n=36)。对照组实施常...目的:探讨早期预见性护理干预在股骨近端钉系统(TFNA)手术中的应用效果。方法:选取广东省清远市中医院2020年1月—2021年1月收治的72例股骨粗隆骨折行TFNA手术患者,根据护理方式不同将其分为观察组(n=36)与对照组(n=36)。对照组实施常规护理,观察组在常规护理基础上实施早期预见性护理干预。对比两组患者的住院时间、护理满意度、并发症发生率以及护理前后焦虑自评量表(SAS)和抑郁自评量表(SDS)评分情况。结果:观察组患者住院时间与并发症发生率低于对照组,满意度高于对照组,差异有统计学意义(P<0.05);护理后,两组患者SAS和SDS评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05);两组患者护理前Harris评分与VAS评分对比,差异无统计学意义(P>0.05),两组患者护理后Harris评分均升高,且观察组高于对照组,差异有统计学意义(P<0.05);两组护理后V A S评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05)。结论:对股骨TFNA手术患者在术中应用早期预见性护理,能够降低患者术后并发症发生率,减少住院时间,减轻患者焦虑、抑郁等不良情绪,减轻患者术后疼痛程度,提升髋关节功能,提高患者护理满意度,值得临床应用。展开更多
Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery effic...Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.展开更多
Periodontitis is a common disease that causes periodontium defects and tooth loss.Controlling inflammation and tissue regeneration are two key strategies in the treatment of periodontitis.Tetrahedral framework nucleic...Periodontitis is a common disease that causes periodontium defects and tooth loss.Controlling inflammation and tissue regeneration are two key strategies in the treatment of periodontitis.Tetrahedral framework nucleic acids can modulate multiple biological behaviors,and thus,their biological applications have been widely explored.In this study,we investigated the effect of tFNAs on periodontium under inflammatory conditions.Lipopolysaccharide and silk ligature were used to induce inflammation in vivo and in vitro.The results displayed that tFNAs decreased the release of pro-inflammatory cytokines and levels of cellular reactive oxygen species in periodontal ligament stem cells,which promoted osteogenic differentiation.Furthermore,animal experiments showed that tFNAs ameliorated the inflammation of the periodontium and protect periodontal tissue,especially reducing alveolar bone absorption by decreasing inflammatory infiltration and inhibiting osteoclast formation.These findings suggest that tFNAs can significantly improve the therapeutic effect of periodontitis and have the great potential significance in the field of periodontal tissue regeneration.展开更多
文摘目的:探讨早期预见性护理干预在股骨近端钉系统(TFNA)手术中的应用效果。方法:选取广东省清远市中医院2020年1月—2021年1月收治的72例股骨粗隆骨折行TFNA手术患者,根据护理方式不同将其分为观察组(n=36)与对照组(n=36)。对照组实施常规护理,观察组在常规护理基础上实施早期预见性护理干预。对比两组患者的住院时间、护理满意度、并发症发生率以及护理前后焦虑自评量表(SAS)和抑郁自评量表(SDS)评分情况。结果:观察组患者住院时间与并发症发生率低于对照组,满意度高于对照组,差异有统计学意义(P<0.05);护理后,两组患者SAS和SDS评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05);两组患者护理前Harris评分与VAS评分对比,差异无统计学意义(P>0.05),两组患者护理后Harris评分均升高,且观察组高于对照组,差异有统计学意义(P<0.05);两组护理后V A S评分均降低,且观察组低于对照组,差异有统计学意义(P<0.05)。结论:对股骨TFNA手术患者在术中应用早期预见性护理,能够降低患者术后并发症发生率,减少住院时间,减轻患者焦虑、抑郁等不良情绪,减轻患者术后疼痛程度,提升髋关节功能,提高患者护理满意度,值得临床应用。
基金supported by National Key R&D Program of China (No. 2019YFA0110600)National Natural Science Foundation of China (No. 81970916)+2 种基金Sichuan Province Youth Science and Technology Innovation Team (No. 2022JDTD0021)Research Funding from West China School/Hospital of Stomatology Sichuan University (No. RCDWJS2021-20)China Postdoctoral Science Foundation (No. 2022TQ0381)。
文摘Synthetic antigen-encoding mRNA plays an increasingly significant role in tumor vaccine technology owing to its antigen-specific immune-activation. However, its immune efficacy is challenged by inferior delivery efficiency and demand for suitable adjuvants. Here, we develop a novel mRNA nanovaccine based on a multifunctional nanocapsule, which is a dual-adjuvant formulation composed of cytosine-phosphateguanine motifs loaded tetrahedral framework nucleic acid(CpG-tFNA) and an immunopeptide murine β-defensin 2(mDF2β). This m RNA nanovaccine successfully achieves intracellular delivery, antigen expression and presentation of dendritic cells, and proliferation of antigen-specific T cells. In a tumor prophylactic vaccination model, it exerts an excellent inhibitory effect on lymphoma occurrence through cellular immunity. This mRNA nanovaccine has promising prophylactic applications in tumors and many other diseases.
基金This study was supported by National Key R&D Program of China(2019YFA0110600)National Natural Science Foundation of China(81970986,81771125).
文摘Periodontitis is a common disease that causes periodontium defects and tooth loss.Controlling inflammation and tissue regeneration are two key strategies in the treatment of periodontitis.Tetrahedral framework nucleic acids can modulate multiple biological behaviors,and thus,their biological applications have been widely explored.In this study,we investigated the effect of tFNAs on periodontium under inflammatory conditions.Lipopolysaccharide and silk ligature were used to induce inflammation in vivo and in vitro.The results displayed that tFNAs decreased the release of pro-inflammatory cytokines and levels of cellular reactive oxygen species in periodontal ligament stem cells,which promoted osteogenic differentiation.Furthermore,animal experiments showed that tFNAs ameliorated the inflammation of the periodontium and protect periodontal tissue,especially reducing alveolar bone absorption by decreasing inflammatory infiltration and inhibiting osteoclast formation.These findings suggest that tFNAs can significantly improve the therapeutic effect of periodontitis and have the great potential significance in the field of periodontal tissue regeneration.
