目的研究转酮醇酶样蛋白1(transketolase-like protein 1,TKTL1)在前列腺癌新辅助内分泌治疗前后组织中的表达及临床意义。方法采用免疫组织化学法检测TKTL1在新辅助内分泌治疗前后前列腺癌组织中的表达.并用单因素方差分析法研究其与...目的研究转酮醇酶样蛋白1(transketolase-like protein 1,TKTL1)在前列腺癌新辅助内分泌治疗前后组织中的表达及临床意义。方法采用免疫组织化学法检测TKTL1在新辅助内分泌治疗前后前列腺癌组织中的表达.并用单因素方差分析法研究其与临床病理因素的关系。结果 TKTL1在新辅助内分泌治疗后的前列腺组织中表达低,差异有统计学意义(P <0.05);TKTL1在前列腺癌中的表达与患者的年龄、肿瘤大小无关(P>0.05),与TNM分期及肿瘤组织分化程度有关(P<0.05)。结论 TKTL1的低表达可以反映新辅助内分泌治疗对前列腺癌的作用,对前列腺癌诊断及判断预后有一定的意义,有可能成为判断前列腺癌预后的一个生物标志物。展开更多
Objective:To study the correlation of Six1, MTDH and TKTL1 expression in laryngeal cancer tissue with serum tumor marker levels and cell proliferation activity.Methods:A total of 48 patients who were diagnosed with pr...Objective:To study the correlation of Six1, MTDH and TKTL1 expression in laryngeal cancer tissue with serum tumor marker levels and cell proliferation activity.Methods:A total of 48 patients who were diagnosed with primary laryngeal cancer in our hospital between June 2013 and December 2016 were collected as observation group, and 50 patients with vocal cord polyp who received laryngoscopy in our hospital during the same period were selected as control group. Fluorescence quantitative PCR was used to determine the expression of Six1, MTDH and TKTL1 as well as and the expression of oncogenes and tumor suppressor genes in lesion tissue of two groups of patients, and the contents of tumor markers in serum were detected. Pearson test was used to evaluate the correlation of Six1, MTDH and TKTL1 expression in laryngeal cancer tissue with serum tumor marker levels and cell proliferation activity.Results: Six1, MTDH, TKTL1, c-myc, STAT3 and ras mRNA expression in lesion tissue as well as tumor markers CA72-4, CA19-9, CYFRA21-1, SCC-Ag and TK1 levels in serum of observation group were higher than those of control group while LKB1, CDKN2 and PTEN mRNA expression were lower than those of control group;Six1, MTDH and TKTL1 expression in laryngeal cancer tissue were positively correlated with serum CA72-4, CA19-9, CYFRA21-1, SCC-Ag and TK1 levels as well as c-myc, STAT3 and ras mRNA expression, and negatively correlated with LKB1, CDKN2 and PTEN mRNA expression.Conclusion:Six1, MTDH and TKTL1 expression increase in laryngeal cancer tissue, and their specific expression are directly correlated with tumor malignancy.展开更多
目的探讨胃癌及胃癌前病变组织中转酮醇酶1(transketolase-like protein 1,TKTL1)表达及其临床意义.方法选取淄博市第一医院2013-2015年消化内科胃镜检查中的正常黏膜(56例)、胃癌前病变(79例)及淄博市第一医院普外科手术并经病理证实...目的探讨胃癌及胃癌前病变组织中转酮醇酶1(transketolase-like protein 1,TKTL1)表达及其临床意义.方法选取淄博市第一医院2013-2015年消化内科胃镜检查中的正常黏膜(56例)、胃癌前病变(79例)及淄博市第一医院普外科手术并经病理证实的胃癌组织(79例),采用免疫组织化学方法检测TKTL1的表达,对TKTL1在各组中的表达水平进行比较,再分析胃癌前病变组中TKTL1的表达与其临床资料的关系,胃癌组中TKTL1的表达与临床病理参数之间的关系.结果在正常黏膜组、胃癌前病变组、胃癌组中的TKTL1表达是逐渐增高的,且差异有统计学意义(P<0.05),其表达与肿瘤大小、患者性别、患者年龄无相关性(P>0.05),而在胃癌组织中,TKTL1的表达与TNM分期及肿瘤浸润深度显著相关(P<0.05).结论T K T L1在胃癌前病变、胃癌组织中有一定高表达率,且胃癌组织中的表达高于癌前病变组,其高表达与胃癌的发生、发展及转移密切相关.展开更多
文摘目的通过检测TKTL1在星形胶质细胞瘤中的表达水平,探讨TKTL1在星形胶质细胞瘤中的意义。方法采用实时荧光定量PCR检测TKTL1在星形胶质细胞瘤中的表达水平,进行免疫组化染色、免疫印迹(Western blot),获得数据进行统计学分析。结果与正常脑组织相比,TKTL1 m RNA表达水平在胶质瘤组织中明显上调,在高级别胶质瘤组织中的表达水平明显高于低级别胶质瘤,差异有统计学意义(P<0.001)。结论 TKTL1在星形胶质细胞瘤组织中比正常脑组织中的表达明显增高,随着肿瘤级别的升高表达增强,推测TKTL1表达与肿瘤细胞的增殖侵袭密切相关。
文摘Objective:To study the correlation of Six1, MTDH and TKTL1 expression in laryngeal cancer tissue with serum tumor marker levels and cell proliferation activity.Methods:A total of 48 patients who were diagnosed with primary laryngeal cancer in our hospital between June 2013 and December 2016 were collected as observation group, and 50 patients with vocal cord polyp who received laryngoscopy in our hospital during the same period were selected as control group. Fluorescence quantitative PCR was used to determine the expression of Six1, MTDH and TKTL1 as well as and the expression of oncogenes and tumor suppressor genes in lesion tissue of two groups of patients, and the contents of tumor markers in serum were detected. Pearson test was used to evaluate the correlation of Six1, MTDH and TKTL1 expression in laryngeal cancer tissue with serum tumor marker levels and cell proliferation activity.Results: Six1, MTDH, TKTL1, c-myc, STAT3 and ras mRNA expression in lesion tissue as well as tumor markers CA72-4, CA19-9, CYFRA21-1, SCC-Ag and TK1 levels in serum of observation group were higher than those of control group while LKB1, CDKN2 and PTEN mRNA expression were lower than those of control group;Six1, MTDH and TKTL1 expression in laryngeal cancer tissue were positively correlated with serum CA72-4, CA19-9, CYFRA21-1, SCC-Ag and TK1 levels as well as c-myc, STAT3 and ras mRNA expression, and negatively correlated with LKB1, CDKN2 and PTEN mRNA expression.Conclusion:Six1, MTDH and TKTL1 expression increase in laryngeal cancer tissue, and their specific expression are directly correlated with tumor malignancy.
文摘目的探讨胃癌及胃癌前病变组织中转酮醇酶1(transketolase-like protein 1,TKTL1)表达及其临床意义.方法选取淄博市第一医院2013-2015年消化内科胃镜检查中的正常黏膜(56例)、胃癌前病变(79例)及淄博市第一医院普外科手术并经病理证实的胃癌组织(79例),采用免疫组织化学方法检测TKTL1的表达,对TKTL1在各组中的表达水平进行比较,再分析胃癌前病变组中TKTL1的表达与其临床资料的关系,胃癌组中TKTL1的表达与临床病理参数之间的关系.结果在正常黏膜组、胃癌前病变组、胃癌组中的TKTL1表达是逐渐增高的,且差异有统计学意义(P<0.05),其表达与肿瘤大小、患者性别、患者年龄无相关性(P>0.05),而在胃癌组织中,TKTL1的表达与TNM分期及肿瘤浸润深度显著相关(P<0.05).结论T K T L1在胃癌前病变、胃癌组织中有一定高表达率,且胃癌组织中的表达高于癌前病变组,其高表达与胃癌的发生、发展及转移密切相关.
