In order to obtain the TLR 4 gene sequence of Hainan Wuzhishan pig and analyze its molecular structure characteristics,its genomic sequence was cloned and sequenced by PCR and subjected to related bioinformatics analy...In order to obtain the TLR 4 gene sequence of Hainan Wuzhishan pig and analyze its molecular structure characteristics,its genomic sequence was cloned and sequenced by PCR and subjected to related bioinformatics analysis with the genomic sequence of the TLR 4 gene of pig in GenBank as a reference sequence.The full-length DNA sequence of Wuzhishan pig TLR 4 gene was 10 435 bp in length,of which CDS was 2 526 bp in length and encoded 841 amino acids.Compared with the common pig TLR 4 gene sequence published by GenBank,there were 38 mutations,of which two mutations were in the CDS region.The results of homology analysis showed that the amino acid sequence of the TLR 4 gene of Wuzhishan pig shared 99.9%,80.7%,79.9%,74.8%,74.7%,73.1%,73.0%,72.7%,62.5% and 43.4% homology with those of common pig,cattle,sheep,dog,horse,macaque,human,chimpanzee,mouse and chicken,respectively.The protein encoded by the TLR 4 of Wuzhishan pig belonged to secreted protein and transmembrane protein.It was hydrophilic,and had 8 glycosylation modification sites,17 serines (ser),7 threonines (Thr) and 8 tyrosine (TYR) phosphorylation sites.The amino acid phylogenetic tree analysis showed that the TLR 4 genes of different species are highly conserved during evolution.This result lays a foundation for further study of the function of TLR 4 gene.展开更多
基金Supported by the Innovative Research Team Project of Natural Science Foundation of Hainan Province(2018CXTD345)Local Science Foundation of National Natural Science Foundation(31560696)+1 种基金Natural Science Foundation of Hainan Province(20153080)Special Fund for the Development of Local Science and Technology Guided by Central Government(ZY2019HN01)
文摘In order to obtain the TLR 4 gene sequence of Hainan Wuzhishan pig and analyze its molecular structure characteristics,its genomic sequence was cloned and sequenced by PCR and subjected to related bioinformatics analysis with the genomic sequence of the TLR 4 gene of pig in GenBank as a reference sequence.The full-length DNA sequence of Wuzhishan pig TLR 4 gene was 10 435 bp in length,of which CDS was 2 526 bp in length and encoded 841 amino acids.Compared with the common pig TLR 4 gene sequence published by GenBank,there were 38 mutations,of which two mutations were in the CDS region.The results of homology analysis showed that the amino acid sequence of the TLR 4 gene of Wuzhishan pig shared 99.9%,80.7%,79.9%,74.8%,74.7%,73.1%,73.0%,72.7%,62.5% and 43.4% homology with those of common pig,cattle,sheep,dog,horse,macaque,human,chimpanzee,mouse and chicken,respectively.The protein encoded by the TLR 4 of Wuzhishan pig belonged to secreted protein and transmembrane protein.It was hydrophilic,and had 8 glycosylation modification sites,17 serines (ser),7 threonines (Thr) and 8 tyrosine (TYR) phosphorylation sites.The amino acid phylogenetic tree analysis showed that the TLR 4 genes of different species are highly conserved during evolution.This result lays a foundation for further study of the function of TLR 4 gene.
基金Postgraduate Scientific Research Innovation Key Project of Hunan Province(CX20210680)Key Scientific Research Project of Hunan Provincial Department of Education(21A0224)+1 种基金Scientific Research Project of Hunan Health Commission(202204083613)CHEN Qihua National Studio for Inheritance of TCM Famous Veteran Doctors(2022)。
基金This work was supported by the fifteenth "Five Years Key Programs" for Science and Technology Development of China (No. 2002BA518A14)the National High Science and Technology Foundation of China (863) (No. 2002AA242011).
