目的：优化蟾酥聚合物胶束的制备工艺。方法：采用薄膜分散-微射流法,以普郎尼克F123和脱氧胆酸钠为聚合物胶束的载体材料,以包封率为主要考察指标,通过星点-效应面法,以F123和脱氧胆酸钠质量比、蟾酥和载体的质量比、水化体积、微射流...目的：优化蟾酥聚合物胶束的制备工艺。方法：采用薄膜分散-微射流法,以普郎尼克F123和脱氧胆酸钠为聚合物胶束的载体材料,以包封率为主要考察指标,通过星点-效应面法,以F123和脱氧胆酸钠质量比、蟾酥和载体的质量比、水化体积、微射流压力、循环次数为主要考察因素,优化蟾酥聚合物胶束的制备工艺。结果：蟾酥聚合物胶束制备的最佳工艺为：F123和脱氧胆酸钠质量比为1.5∶1,蟾酥和载体的质量比为45∶1,水化体积为9 m L,微射流压力为120 MPa,循环次数为7次;蟾酥聚合物胶束在透射电镜下观察呈类球形,平均粒径为120 nm,包封率为94.12%,载药量为0.14%。结论：该制备工艺稳定可行,重复性好,可用于蟾酥聚合物胶束的制备。展开更多
1 Introduction Sea anemone toxins are polypeptide neurotoxins. The symptoms of the toxic animals exhibits contracture and paralysis of their limbs. It is evident that the peripheral nerve and muscle are important targ...1 Introduction Sea anemone toxins are polypeptide neurotoxins. The symptoms of the toxic animals exhibits contracture and paralysis of their limbs. It is evident that the peripheral nerve and muscle are important targets of the toxin. Up to now, it is still a controversial topic about which part of the N-M preparation is affected. Shapiro considered that the venom from Condylactic gigantea was active on crayfish nerve but not on muscle membrane. Metezeau et al. used curarized and denervated frog sartofius muscles, and found that ATX-Ⅱ from Anemonia sulcata acted on motor nerve terminals but not directly on muscle fibres. Erxleben et al. proved that ATX-Ⅱ could act on frog skeletal muscle directly展开更多
文摘目的：优化蟾酥聚合物胶束的制备工艺。方法：采用薄膜分散-微射流法,以普郎尼克F123和脱氧胆酸钠为聚合物胶束的载体材料,以包封率为主要考察指标,通过星点-效应面法,以F123和脱氧胆酸钠质量比、蟾酥和载体的质量比、水化体积、微射流压力、循环次数为主要考察因素,优化蟾酥聚合物胶束的制备工艺。结果：蟾酥聚合物胶束制备的最佳工艺为：F123和脱氧胆酸钠质量比为1.5∶1,蟾酥和载体的质量比为45∶1,水化体积为9 m L,微射流压力为120 MPa,循环次数为7次;蟾酥聚合物胶束在透射电镜下观察呈类球形,平均粒径为120 nm,包封率为94.12%,载药量为0.14%。结论：该制备工艺稳定可行,重复性好,可用于蟾酥聚合物胶束的制备。
基金Project supported by the National Natural Science Foundation of China
文摘1 Introduction Sea anemone toxins are polypeptide neurotoxins. The symptoms of the toxic animals exhibits contracture and paralysis of their limbs. It is evident that the peripheral nerve and muscle are important targets of the toxin. Up to now, it is still a controversial topic about which part of the N-M preparation is affected. Shapiro considered that the venom from Condylactic gigantea was active on crayfish nerve but not on muscle membrane. Metezeau et al. used curarized and denervated frog sartofius muscles, and found that ATX-Ⅱ from Anemonia sulcata acted on motor nerve terminals but not directly on muscle fibres. Erxleben et al. proved that ATX-Ⅱ could act on frog skeletal muscle directly
