In present paper, the author established the concept of PMCN-rate arid suggests in complex conditions it is necessary to introduce the another index, i. e. frequency of tetrads with polymicronuclei (PMCN) , into the T...In present paper, the author established the concept of PMCN-rate arid suggests in complex conditions it is necessary to introduce the another index, i. e. frequency of tetrads with polymicronuclei (PMCN) , into the Trad -MCN test.展开更多
In 2007- 2008,a systematic survey,collection and arrangement was carried out for agricultural biological resources and traditional cultural knowledge of Hani People in 8 counties,15 towns,and 23 village committees of ...In 2007- 2008,a systematic survey,collection and arrangement was carried out for agricultural biological resources and traditional cultural knowledge of Hani People in 8 counties,15 towns,and 23 village committees of Yunnan Province. A total of 299 samples were obtained about agricultural biological resources related to production and living of Hani People. According to purpose of utilization,samples were divided into grain crops,medicinal plants,vegetables,fruit trees,and oil crops,taking up 48. 2%,21. 7%,18. 4%,7. 7%,and 2. 0% of the samples respectively. The survey indicated that planting industry and breeding industry take up the dominant role in rural social economy of Hani People,so agricultural biological resources are the fundamental means of production maintaining rural social development of Hani People.The current situation of agricultural biological resources of Hani People in Yunnan,reasons for growth and decline were analyzed,and the utilization,protection and development of agricultural biological resources were discussed.展开更多
Objective To understand the mechanism of invasion by Legionella dumoffii. Methods The L. dumoffii strain Tex-KL was mutated using the Tn903 derivative, Tn903 d IIlac Z. After screening 799 transposon insertion mutants...Objective To understand the mechanism of invasion by Legionella dumoffii. Methods The L. dumoffii strain Tex-KL was mutated using the Tn903 derivative, Tn903 d IIlac Z. After screening 799 transposon insertion mutants, we isolated one defective mutant. We then constructed the gene-disrupted mutant, KL16, and studied its invasion of and intracellular growth in He La and A549 cells, and in A/J mice survival experiments. The structure of traC-traD operon was analyzed by RT-PCR. Results The transposon insertion was in a gene homologous to Salmonella typhi tra C, which is required for the assembly of F pilin into the mature F pilus structure and for conjugal DNA transmission. Results from RT-PCR suggested that the traC-traD region formed an operon. We found that when the tra C gene was disrupted, invasion and intracellular growth of L. dumoffii Tex-KL were impaired in human epithelial cells. When mice were infected by intranasal inoculation with a tra C deficient mutant, their survival significantly increased when compared to mice infected with the wild-type strain. Conclusion Our results indicated that the traC-traD operon is required for the invasion and intracellular growth abilities of L. dumoffii Tex-KL in epithelial cells.展开更多
In the context of China's efforts to establish a global network of free trade areas and diversify its export products, this study explores the impact of trade agreement depth on China's export diversification....In the context of China's efforts to establish a global network of free trade areas and diversify its export products, this study explores the impact of trade agreement depth on China's export diversification. Building upon a trade model with multiproduct firms, we discover that the effect of trade agreement depth on export diversification is multifaceted, depending on the relative magnitude of the “market expansion effect” and the “competition intensification effect.” Through empirical analysis of China's exports to 132 countries (or regions) from 2000 to 2015, we find that the deepening of trade agreements affected China's export diversification negatively. This negative correlation was predominantly due to the similarity in comparative advantages between China and its trade partners, leading to the “competition intensification effect” overshadowing the “market expansion effect.” We also note that “natural” agreements, when deepened, were more likely to affect China's export diversification adversely than their “non-natural” counterparts. Moreover, as export diversification increased, the marginal impact of deepening trade agreements exhibited an inverted U-shaped trajectory.展开更多
To evaluate the efficacy of a novel compound derived from Traditional Chinese Medicine (TCM) for treatment of alveolar echinococcosis in the Kuming mouse Methods The compound was extracted from TCM by serial procedu...To evaluate the efficacy of a novel compound derived from Traditional Chinese Medicine (TCM) for treatment of alveolar echinococcosis in the Kuming mouse Methods The compound was extracted from TCM by serial procedures including amalgamation of TCM drugs, boiling in a beaker, cold condensation using a reflux instrument, evaporation by mild heating and finally, formation of a deep brown powder An animal model of alveolar echinococcosis was established concurrently A bit of alveococcus tissue derived from alveolar echinococcosis in the mouse was transplanted into the peritoneal cavity of the musculus of a Kuming mouse The duration of infection after inoculation was classified as either 1 week or 10 weeks; the animals were again divided into treated groups and control groups Estimation of the alveococcus inhibitory rate and electron microscopic observation served as the main methods for assessment of efficacy The infected mice in the treated groups were intra gastrically administered once per day with the TCM extractive powder at doses of 20 mg·kg 1 for three months continuously All the animals were sacrificed and examined half a month after the end of TCM treatment Results Total wet weight of alveococcus collected from the 9 mice in the treated group after one week of infection was 37 8 g ( ±s , 4 196±2 090 g), which was markedly lower than the amount collected from 10 mice of the control group (121 3 g, ±s , 12 1±4 3 g) Therefore, the inhibitory rate of the alveococcus was 65 7% ( P 【0 01 ) Similarly, the total wet weight of alveococcus from 7 mice of the treated group after 10 weeks of infection was 4 3 g ( ±s , 0 62±1 21 g), which was much lower than the amount collected from 6 mice of the control group (17 85 g, ±s , 2 93±3 28 g) The inhibitory rate of alveococcus was 80 6% ( P 【0 01) Furthermore, the ultrastructural appearances of alveococcus showed obvious differences between the two groups Conclusion TCM extractive powder for treatment of murine alveococcosis is considered to be a hopeful anti echinococcus compound it is a topic for further study展开更多
Irritable bowel syndrome(IBS)is a common,chronic,functional gastrointestinal disorder with a high incidence rate in the general population,and it is common among the Chinese population.The pathophysiology,etiology and...Irritable bowel syndrome(IBS)is a common,chronic,functional gastrointestinal disorder with a high incidence rate in the general population,and it is common among the Chinese population.The pathophysiology,etiology and pathogenesis of IBS are poorly understood,with no evidence of inflammatory,anatomic,metabolic,or neoplastic factors to explain the symptoms.Treatment approaches are mainly focused on symptommanagement to maintain everyday functioning and to improve quality of life for patients with IBS.However,prescribed medications often result in significant side effects,and many IBS sufferers(particularly Chinese)do not improve.Instead of taking a variety of conventional medications,many have turned to taking traditional Chinese medicine or integrated Chinese and Western medicine for remedy.A number of clinical trials have shown that Chinese herbal,acupuncture or integrative therapies presented improved treatment outcomes and reduced side effects in IBS patients.The purpose of this review article is to examine the treatment approaches of IBS that have been published in recent years,especially in China,to assess the possible differences in treating IBS between China and other countries.This would provide some useful information of unique treatment approach in clinical practice for physicians in the management of IBS in China,thus offering more treatment options for IBS patients with potentially better treatment outcomes while reducing the medical cost burden.展开更多
文摘In present paper, the author established the concept of PMCN-rate arid suggests in complex conditions it is necessary to introduce the another index, i. e. frequency of tetrads with polymicronuclei (PMCN) , into the Trad -MCN test.
基金Supported by Special Project of Basic Scientific and Technological Research of the State Ministry of Science and Technology in the Eleventh Five-Year Plan Period(2006FY110700)China Agriculture Research System(CARS-25)National Crop Germplasm Resource Platform
文摘In 2007- 2008,a systematic survey,collection and arrangement was carried out for agricultural biological resources and traditional cultural knowledge of Hani People in 8 counties,15 towns,and 23 village committees of Yunnan Province. A total of 299 samples were obtained about agricultural biological resources related to production and living of Hani People. According to purpose of utilization,samples were divided into grain crops,medicinal plants,vegetables,fruit trees,and oil crops,taking up 48. 2%,21. 7%,18. 4%,7. 7%,and 2. 0% of the samples respectively. The survey indicated that planting industry and breeding industry take up the dominant role in rural social economy of Hani People,so agricultural biological resources are the fundamental means of production maintaining rural social development of Hani People.The current situation of agricultural biological resources of Hani People in Yunnan,reasons for growth and decline were analyzed,and the utilization,protection and development of agricultural biological resources were discussed.
基金supported by the National Natural Scientific Foundation(No.81201251)from the Ministry of Science and Technology of the People’s Republic of Chinathe Priority Project on Infectious Disease Control and Prevention(No.2012ZX10004215 and 2013ZX10004-610-007)from the Ministry of Health and the Ministry of Science and Technology of the People’s Republic of Chinathe Science Foundation for the State Key Laboratory for Infectious Disease Prevention and Control from China(Grant No.2015SKLID508 and 2011SKLID202)
文摘Objective To understand the mechanism of invasion by Legionella dumoffii. Methods The L. dumoffii strain Tex-KL was mutated using the Tn903 derivative, Tn903 d IIlac Z. After screening 799 transposon insertion mutants, we isolated one defective mutant. We then constructed the gene-disrupted mutant, KL16, and studied its invasion of and intracellular growth in He La and A549 cells, and in A/J mice survival experiments. The structure of traC-traD operon was analyzed by RT-PCR. Results The transposon insertion was in a gene homologous to Salmonella typhi tra C, which is required for the assembly of F pilin into the mature F pilus structure and for conjugal DNA transmission. Results from RT-PCR suggested that the traC-traD region formed an operon. We found that when the tra C gene was disrupted, invasion and intracellular growth of L. dumoffii Tex-KL were impaired in human epithelial cells. When mice were infected by intranasal inoculation with a tra C deficient mutant, their survival significantly increased when compared to mice infected with the wild-type strain. Conclusion Our results indicated that the traC-traD operon is required for the invasion and intracellular growth abilities of L. dumoffii Tex-KL in epithelial cells.
基金The authors are grateful for support from the China National Social Science Foundation(Nos.19BJY192 and 23BGL151).
文摘In the context of China's efforts to establish a global network of free trade areas and diversify its export products, this study explores the impact of trade agreement depth on China's export diversification. Building upon a trade model with multiproduct firms, we discover that the effect of trade agreement depth on export diversification is multifaceted, depending on the relative magnitude of the “market expansion effect” and the “competition intensification effect.” Through empirical analysis of China's exports to 132 countries (or regions) from 2000 to 2015, we find that the deepening of trade agreements affected China's export diversification negatively. This negative correlation was predominantly due to the similarity in comparative advantages between China and its trade partners, leading to the “competition intensification effect” overshadowing the “market expansion effect.” We also note that “natural” agreements, when deepened, were more likely to affect China's export diversification adversely than their “non-natural” counterparts. Moreover, as export diversification increased, the marginal impact of deepening trade agreements exhibited an inverted U-shaped trajectory.
基金ThisworkwassupportedbyNaturalScienceFoundationofGansuProvincialScienceandTechnicalBureau (NO ZR 94 86)
文摘To evaluate the efficacy of a novel compound derived from Traditional Chinese Medicine (TCM) for treatment of alveolar echinococcosis in the Kuming mouse Methods The compound was extracted from TCM by serial procedures including amalgamation of TCM drugs, boiling in a beaker, cold condensation using a reflux instrument, evaporation by mild heating and finally, formation of a deep brown powder An animal model of alveolar echinococcosis was established concurrently A bit of alveococcus tissue derived from alveolar echinococcosis in the mouse was transplanted into the peritoneal cavity of the musculus of a Kuming mouse The duration of infection after inoculation was classified as either 1 week or 10 weeks; the animals were again divided into treated groups and control groups Estimation of the alveococcus inhibitory rate and electron microscopic observation served as the main methods for assessment of efficacy The infected mice in the treated groups were intra gastrically administered once per day with the TCM extractive powder at doses of 20 mg·kg 1 for three months continuously All the animals were sacrificed and examined half a month after the end of TCM treatment Results Total wet weight of alveococcus collected from the 9 mice in the treated group after one week of infection was 37 8 g ( ±s , 4 196±2 090 g), which was markedly lower than the amount collected from 10 mice of the control group (121 3 g, ±s , 12 1±4 3 g) Therefore, the inhibitory rate of the alveococcus was 65 7% ( P 【0 01 ) Similarly, the total wet weight of alveococcus from 7 mice of the treated group after 10 weeks of infection was 4 3 g ( ±s , 0 62±1 21 g), which was much lower than the amount collected from 6 mice of the control group (17 85 g, ±s , 2 93±3 28 g) The inhibitory rate of alveococcus was 80 6% ( P 【0 01) Furthermore, the ultrastructural appearances of alveococcus showed obvious differences between the two groups Conclusion TCM extractive powder for treatment of murine alveococcosis is considered to be a hopeful anti echinococcus compound it is a topic for further study
文摘Irritable bowel syndrome(IBS)is a common,chronic,functional gastrointestinal disorder with a high incidence rate in the general population,and it is common among the Chinese population.The pathophysiology,etiology and pathogenesis of IBS are poorly understood,with no evidence of inflammatory,anatomic,metabolic,or neoplastic factors to explain the symptoms.Treatment approaches are mainly focused on symptommanagement to maintain everyday functioning and to improve quality of life for patients with IBS.However,prescribed medications often result in significant side effects,and many IBS sufferers(particularly Chinese)do not improve.Instead of taking a variety of conventional medications,many have turned to taking traditional Chinese medicine or integrated Chinese and Western medicine for remedy.A number of clinical trials have shown that Chinese herbal,acupuncture or integrative therapies presented improved treatment outcomes and reduced side effects in IBS patients.The purpose of this review article is to examine the treatment approaches of IBS that have been published in recent years,especially in China,to assess the possible differences in treating IBS between China and other countries.This would provide some useful information of unique treatment approach in clinical practice for physicians in the management of IBS in China,thus offering more treatment options for IBS patients with potentially better treatment outcomes while reducing the medical cost burden.
